P79106 (PAFA2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Platelet-activating factor acetylhydrolase 2, cytoplasmic
EC=3.1.1.47

Alternative name(s):
Serine-dependent phospholipase A2
Short name=SD-PLA2

Gene names

Name:

PAFAH2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme.
Catalytic activity	1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed at highest levels in liver and at lower levels in other tissues.
Sequence similarities	Belongs to the serine esterase family.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-alkyl-2-acetylglycerophosphocholine esterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Platelet-activating factor acetylhydrolase 2, cytoplasmic		PRO_0000090382
Sites
	Active site	236	1	Nucleophile By similarity
	Active site	259	1	Charge relay system By similarity
	Active site	314	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	P79106 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 1DEACA2ADFA4CEA8	FASTA	392	43,865
	10 20 30 40 50 60 MGVNQSVSFP PVTGPHLVGC GDVMEGQSLQ GSFFRLFYPC QEAEETSEQP LWIPRYEYCA 70 80 90 100 110 120 GLAEYLKFNK RWGGLLFNLG VGSCRLPVSW NGPFKTKDSG YPLIIFSHGM GAFRTVYSAF 130 140 150 160 170 180 CMELASRGFV VAVPEHRDGS AAATCFCKQT PEENQPDNEA LKEEWIPHRQ IEEGEKEFYV 190 200 210 220 230 240 RNYQVHQRVS ECVRVLKILQ EVTAGQAVLN ILPGGLDLMT LKGGIDVSRV AVMGHSFGGA 250 260 270 280 290 300 TAILALAKEM QFRCAVALDA WMFPLEHDFY PTARGPIFFI NAEKFQTVET VNLMKKICDQ 310 320 330 340 350 360 HHQSRIITVL GSVHRSLTDF VFVAGNWISK FFSSHTRGSL DPYEGQETVV RAMLAFLQKH 370 380 390 LDLKEDYDQW NNFIEGIGPS LTPGAPHHLS SL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and expression of intracellular platelet-activating factor (PAF) acetylhydrolase II. Its homology with plasma PAF acetylhydrolase." Hattori K., Adachi H., Matsuzawa A., Yamamoto K., Tsujimoto M., Aoki J., Hattori M., Arai H., Inoue K. J. Biol. Chem. 271:33032-33038(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Kidney.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[4]	"Platelet-activating factor acetylhydrolases." Stafforini D.M., McIntyre T.M., Zimmerman G.A., Prescott S.M. J. Biol. Chem. 272:17895-17898(1997) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87559 mRNA. Translation: BAA13419.1. BT020849 mRNA. Translation: AAX08866.1. BC105148 mRNA. Translation: AAI05149.1.
IPI	IPI00710343.
RefSeq	NP_776995.1. NM_174570.2.
UniGene	Bt.103232. Bt.4013.
3D structure databases
ProteinModelPortal	P79106.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000006727.
Protocols and materials databases
DNASU	282299.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000006727; ENSBTAP00000006727; ENSBTAG00000005105. ENSBTAT00000065543; ENSBTAP00000056542; ENSBTAG00000005105.
GeneID	282299.
KEGG	bta:282299.
Organism-specific databases
CTD	5051.
Phylogenomic databases
eggNOG	COG4188.
GeneTree	ENSGT00390000005233.
HOGENOM	HOG000008053.
HOVERGEN	HBG001322.
InParanoid	P79106.
KO	K01062.
OMA	CMELASR.
OrthoDB	EOG45DWPG.
Enzyme and pathway databases
BioCyc	CATTLE:282299-MONOMER.
Family and domain databases
InterPro	IPR016715. Ac_Ohase_PAF. IPR005065. PAF_acetylhydro. [Graphical view]
PANTHER	PTHR10272. PTHR10272. 1 hit.
Pfam	PF03403. PAF-AH_p_II. 1 hit. [Graphical view]
PIRSF	PIRSF018169. PAF_acetylhydrolase. 1 hit.
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806100.

Entry information

Entry name

PAFA2_BOVIN

Accession

Primary (citable) accession number: P79106
Secondary accession number(s): Q5E9S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program