P79105 (S10AC_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A12

Alternative name(s):
Calcium-binding protein in amniotic fluid 1
Short name=CAAF1
Calgranulin-C
Short name=CAGC
Cornea-associated antigen
Short name=CO-AG
Extracellular newly identified RAGE-binding protein
Short name=EN-RAGE
RAGE-binding protein
S100 calcium-binding protein A12

Gene names

Name:	S100A12
Synonyms:	CAAF1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds calcium, zinc and copper. Presence of zinc increases the affinity for calcium. Plays an important role in the inflammatory response. Interaction with AGER on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators By similarity.
Subunit structure	Homodimer. Homooligomer (tetramer or hexamer) in the presence of calcium and zinc ions. Interacts with AGER and both calcium and zinc are essential for the interaction By similarity. Ref.2
Tissue specificity	Up-regulated in stimulated inflammatory effector cells. Ref.2
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Biological process	Inflammatory response
Domain	Repeat
Ligand	Calcium Copper Metal-binding Zinc
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW mast cell degranulation Non-traceable author statement. Source: UniProtKB
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro drug binding Inferred from direct assay. Source: UniProtKB protein binding Inferred from physical interaction Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 92	91	Protein S100-A12		PRO_0000144015
Regions
	Domain	13 – 48	36	EF-hand 1
	Domain	49 – 84	36	EF-hand 2
	Calcium binding	19 – 32	14	1; low affinity By similarity
	Calcium binding	62 – 73	12	2; high affinity By similarity
Experimental info
	Sequence conflict	31	1	R → Y AA sequence Ref.2
	Sequence conflict	37	1	I → G AA sequence Ref.2
	Sequence conflict	66	1	D → K AA sequence Ref.4
	Sequence conflict	70	1	S → V AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P79105 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 060233882D7C901F	FASTA	92	10,685
	10 20 30 40 50 60 MTKLEDHLEG IINIFHQYSV RVGHFDTLNK RELKQLITKE LPKTLQNTKD QPTIDKIFQD 70 80 90 LDADKDGAVS FEEFVVLVSR VLKTAHIDIH KE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel calcium-binding protein in amniotic fluid, CAAF1: its molecular cloning and tissue distribution." Hitomi J., Yamaguchi K., Kikuchi Y., Kimura T., Maruyama K., Nagasaki K. J. Cell Sci. 109:805-815(1996) [PubMed: 8718672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Oesophagus.
[2]	"RAGE mediates a novel proinflammatory axis: a central cell surface receptor for S100/calgranulin polypeptides." Hofmann M.A., Drury S., Fu C., Qu W., Taguchi A., Lu Y., Avila C., Kambham N., Bierhaus A., Nawroth P., Neurath M.F., Slattery T., Beach D., McClary J., Nagashima M., Morser J., Stern D., Schmidt A.M. Cell 97:889-901(1999) [PubMed: 10399917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-51 AND 66-83, INTERACTION WITH AGER, TISSUE SPECIFICITY. Tissue: Lung.
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.
[4]	"Amino acid sequence of an immunogenic corneal stromal protein." Liu S.H., Gottsch J.D. Invest. Ophthalmol. Vis. Sci. 37:944-948(1996) [PubMed: 8603881] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-71. Tissue: Corneal stroma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D49548 mRNA. Translation: BAA08496.1. AF011757 mRNA. Translation: AAB65423.1. BC102542 mRNA. Translation: AAI02543.1.
IPI	IPI00713229.
RefSeq	NP_777076.1. NM_174651.2.
UniGene	Bt.101361. Bt.357.
3D structure databases
ProteinModelPortal	P79105.
SMR	P79105. Positions 2-91.
ModBase	Search...
Protein-protein interaction databases
STRING	P79105.
Proteomic databases
PRIDE	P79105.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000034108; ENSBTAP00000034009; ENSBTAG00000012638.
GeneID	282467.
KEGG	bta:282467.
Organism-specific databases
CTD	6283.
Phylogenomic databases
eggNOG	maNOG21154.
GeneTree	ENSGT00600000084283.
HOVERGEN	HBG001479.
InParanoid	P79105.
OMA	QDLDADK.
OrthoDB	EOG4FFD3C.
PhylomeDB	P79105.
Family and domain databases
InterPro	IPR018248. EF-hand. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR001751. S100/CaBP-9k_CS. IPR013787. S100_Ca-bd_sub. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00036. efhand. 1 hit. PF01023. S_100. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. PS00303. S100_CABP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

S10AC_BOVIN

Accession

Primary (citable) accession number: P79105
Secondary accession number(s): Q3T070, Q9TR16

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program