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Protein

Cleavage and polyadenylation specificity factor subunit 3

Gene

CPSF3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi71 – 711Zinc 1By similarity
Metal bindingi73 – 731Zinc 1By similarity
Metal bindingi75 – 751Zinc 2By similarity
Metal bindingi76 – 761Zinc 2By similarity
Metal bindingi158 – 1581Zinc 1By similarity
Metal bindingi179 – 1791Zinc 1By similarity
Metal bindingi179 – 1791Zinc 2By similarity
Active sitei396 – 3961Proton donorSequence analysis
Metal bindingi418 – 4181Zinc 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Ribonucleoprotein

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-109688. Cleavage of Growing Transcript in the Termination Region.
R-BTA-72163. mRNA Splicing - Major Pathway.
R-BTA-72187. mRNA 3'-end processing.
R-BTA-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage and polyadenylation specificity factor subunit 3 (EC:3.1.27.-By similarity)
Alternative name(s):
Cleavage and polyadenylation specificity factor 73 kDa subunit
Short name:
CPSF 73 kDa subunit
mRNA 3'-end-processing endonuclease CPSF-73
Gene namesi
Name:CPSF3
Synonyms:CPSF73
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 684683Cleavage and polyadenylation specificity factor subunit 3PRO_0000074399Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Cross-linki462 – 462Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki465 – 465Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki545 – 545Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei659 – 6591PhosphoserineBy similarity
Modified residuei681 – 6811PhosphothreonineBy similarity

Post-translational modificationi

Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP79101.
PRIDEiP79101.

Expressioni

Gene expression databases

BgeeiENSBTAG00000019735.

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Interacts with CPSF2, CSTF2 and SYMPK. Interacts with TUT1; the interaction is direct and mediates the recruitment of the CPSF complex on the 3'UTR of pre-mRNAs. Interacts with WDR33. Interacts with ZC3H3.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
E2P031202EBI-7894416,EBI-1779322From a different organism.

Protein-protein interaction databases

IntActiP79101. 1 interaction.
MINTiMINT-8393774.
STRINGi9913.ENSBTAP00000026303.

Structurei

3D structure databases

ProteinModelPortaliP79101.
SMRiP79101. Positions 7-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1137. Eukaryota.
COG1236. LUCA.
GeneTreeiENSGT00840000129795.
HOGENOMiHOG000203394.
HOVERGENiHBG051107.
InParanoidiP79101.
KOiK14403.
OMAiHTMNSNI.
OrthoDBiEOG091G03UN.
TreeFamiTF105643.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR022712. Beta_Casp.
IPR021718. CPSF73-100_C.
IPR001279. Metallo-B-lactamas.
IPR011108. RMMBL.
[Graphical view]
PfamiPF10996. Beta-Casp. 1 hit.
PF11718. CPSF73-100_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PF07521. RMMBL. 1 hit.
[Graphical view]
SMARTiSM01027. Beta-Casp. 1 hit.
SM01098. CPSF73-100_C. 1 hit.
SM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P79101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIPAEESD QLLIRPLGAG QEVGRSCIIL EFKGRKIMLD CGIHPGLEGM
60 70 80 90 100
DALPYIDLID PAEIDLLLIS HFHLDHCGAL PWFLQKTSFK GRTFMTHATK
110 120 130 140 150
AIYRWLLSDY VKVSNISADD MLYTETDLEE SMDKIETINF HEVKEVAGIK
160 170 180 190 200
FWCYHAGHVL GAAMFMIEIA GVKLLYTGDF SRQEDRHLMA AEIPNIKPDI
210 220 230 240 250
LIIESTYGTH IHEKREEREA RFCNTVHDIV NRGGRGLIPV FALGRAQELL
260 270 280 290 300
LILDEYWQNH PELHDIPIYY ASSLAKKCMA VYQTYVNAMN DKIRKQININ
310 320 330 340 350
NPFVFKHISN LKSMDHFDDI GPSVVMASPG MMQSGLSREL FESWCTDKRN
360 370 380 390 400
GVIIAGYCVE GTLAKHIMSE PEEITTMSGQ KLPLKMSVDY ISFSAHTDYQ
410 420 430 440 450
QTSEFIRALK PPHVILVHGE QNEMARLKAA LIREYEDNDE VHIEVHNPRN
460 470 480 490 500
TEAVTLNFRG EKLAKVMGFL ADKKPEQGQR VSGILVKRNF NYHILSPCDL
510 520 530 540 550
SNYTDLAMST VKQTQAIPYT GPFNLLYYQL QKLTGDVEEL EIQEKPALKV
560 570 580 590 600
FKNITVIQEP GMVVLEWLAN PSNDMYADTV TTVILEVQSN PKIRKGAVQK
610 620 630 640 650
VSKKLEMHVY SKRLEIMLQD IFGEDCVSVK DGSILSVTVD GKTANINLET
660 670 680
RTVECEEGSE DDESLREMVE LAAQRLYEAL TPVH
Length:684
Mass (Da):77,488
Last modified:May 1, 1997 - v1
Checksum:i17A0C4FED2B8172E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95906 mRNA. Translation: CAA65151.1.
BC104553 mRNA. Translation: AAI04554.1.
RefSeqiNP_776709.1. NM_174284.2.
UniGeneiBt.5045.

Genome annotation databases

EnsembliENSBTAT00000026303; ENSBTAP00000026303; ENSBTAG00000019735.
GeneIDi281712.
KEGGibta:281712.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95906 mRNA. Translation: CAA65151.1.
BC104553 mRNA. Translation: AAI04554.1.
RefSeqiNP_776709.1. NM_174284.2.
UniGeneiBt.5045.

3D structure databases

ProteinModelPortaliP79101.
SMRiP79101. Positions 7-459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP79101. 1 interaction.
MINTiMINT-8393774.
STRINGi9913.ENSBTAP00000026303.

Proteomic databases

PaxDbiP79101.
PRIDEiP79101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026303; ENSBTAP00000026303; ENSBTAG00000019735.
GeneIDi281712.
KEGGibta:281712.

Organism-specific databases

CTDi51692.

Phylogenomic databases

eggNOGiKOG1137. Eukaryota.
COG1236. LUCA.
GeneTreeiENSGT00840000129795.
HOGENOMiHOG000203394.
HOVERGENiHBG051107.
InParanoidiP79101.
KOiK14403.
OMAiHTMNSNI.
OrthoDBiEOG091G03UN.
TreeFamiTF105643.

Enzyme and pathway databases

ReactomeiR-BTA-109688. Cleavage of Growing Transcript in the Termination Region.
R-BTA-72163. mRNA Splicing - Major Pathway.
R-BTA-72187. mRNA 3'-end processing.
R-BTA-77595. Processing of Intronless Pre-mRNAs.

Gene expression databases

BgeeiENSBTAG00000019735.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR022712. Beta_Casp.
IPR021718. CPSF73-100_C.
IPR001279. Metallo-B-lactamas.
IPR011108. RMMBL.
[Graphical view]
PfamiPF10996. Beta-Casp. 1 hit.
PF11718. CPSF73-100_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PF07521. RMMBL. 1 hit.
[Graphical view]
SMARTiSM01027. Beta-Casp. 1 hit.
SM01098. CPSF73-100_C. 1 hit.
SM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCPSF3_BOVIN
AccessioniPrimary (citable) accession number: P79101
Secondary accession number(s): Q3MHX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.