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Protein

Short transient receptor potential channel 4

Gene

TRPC4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion. Acts as a cell-cell contact-dependent endothelial calcium entry channel (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-BTA-3295583. TRP channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Short transient receptor potential channel 4
Short name:
TrpC4
Alternative name(s):
Capacitative calcium entry channel 1
Short name:
CCE1
Gene namesi
Name:TRPC4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 12

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 329329CytoplasmicSequence analysisAdd
BLAST
Transmembranei330 – 35021HelicalSequence analysisAdd
BLAST
Topological domaini351 – 36212ExtracellularSequence analysisAdd
BLAST
Transmembranei363 – 38321HelicalSequence analysisAdd
BLAST
Topological domaini384 – 43653CytoplasmicSequence analysisAdd
BLAST
Transmembranei437 – 45721HelicalSequence analysisAdd
BLAST
Topological domaini458 – 46912ExtracellularSequence analysisAdd
BLAST
Transmembranei470 – 49021HelicalSequence analysisAdd
BLAST
Topological domaini491 – 51121CytoplasmicSequence analysisAdd
BLAST
Transmembranei512 – 53221HelicalSequence analysisAdd
BLAST
Topological domaini533 – 59967ExtracellularSequence analysisAdd
BLAST
Transmembranei600 – 62021HelicalSequence analysisAdd
BLAST
Topological domaini621 – 979359CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 979979Short transient receptor potential channel 4PRO_0000215313Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei961 – 9611Phosphotyrosine; by FYNBy similarity
Modified residuei974 – 9741Phosphotyrosine; by FYNBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP79100.
PRIDEiP79100.

Expressioni

Tissue specificityi

Expressed in adrenal gland. Lower expression in heart and retina. Also expressed in testis. The short isoform is specifically expressed in the adrenal gland.

Interactioni

Subunit structurei

Homotetramer and heterotetramer with TRPC1 and/or TRPC5 (By similarity). Isoform alpha associates with inositol 1,4,5-triphosphate receptor (ITPR). Interacts with (via the PDZ-binding domain) with NHERF (By similarity). Interacts with MX1 and RNF24 (By similarity). Interacts (via CIRB domain) with SESTD1 (via the spectrin 1 repeat) (By similarity). Interacts with CDH5 and CTNNB1 (By similarity). Interacts (via protein 4.1-binding domain) with EPB41L2 (By similarity). Interacts with TRPC4AP (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012378.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati31 – 6030ANK 1Add
BLAST
Repeati69 – 9729ANK 2Add
BLAST
Repeati98 – 12427ANK 3Add
BLAST
Repeati141 – 17030ANK 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 17286Multimerization domainBy similarityAdd
BLAST
Regioni254 – 30451Multimerization domainBy similarityAdd
BLAST
Regioni615 – 979365Binds to ITPR1, ITPR2 and ITPR3By similarityAdd
BLAST
Regioni977 – 9793PDZ-binding domainBy similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili223 – 26038Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3609. Eukaryota.
ENOG410XQ0Y. LUCA.
GeneTreeiENSGT00760000119180.
HOGENOMiHOG000020589.
HOVERGENiHBG068337.
InParanoidiP79100.
KOiK04967.
OrthoDBiEOG72G17P.
TreeFamiTF313147.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005460. TRPC4_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERiPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF25. PTHR10117:SF25. 1 hit.
PfamiPF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSiPR01097. TRNSRECEPTRP.
PR01645. TRPCHANNEL4.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: P79100-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK
60 70 80 90 100
SLEEAEIYFK ININCIDPLG RTALLIAIEN ENLELIELLL SFNVYVGDAL
110 120 130 140 150
LHAIRKEVVG AVELLLNHKK PSGEKQVPPI LLDKQFSEFT PDITPIILAA
160 170 180 190 200
HTNNYEIIKL LVQKGVSVPR PHEVRCNCVE CVSSSDVDSL RHSRSRLNIY
210 220 230 240 250
KALASPSLIA LSSEDPFLTA FQLSWELQEL SKVENEFKSE YEELSRQCKQ
260 270 280 290 300
FAKDLLDQTR SSRELEIILN YRDDNSLLEE QSGNDLARLK LAIKYRQKEF
310 320 330 340 350
VAQPNCQQLL ASRWYDEFPG WRRRHWAVKM VTCFIVGLLF PVFSVCYLIA
360 370 380 390 400
PKSPLGLFIR KPFIKFICHT ASYLTFLFLL LLASQHIDRS DLNRQGPPPT
410 420 430 440 450
IVEWMILPWV LGFIWGEIKQ MWDGGLQDYI HDWWNLMDFV MNSLYLATIS
460 470 480 490 500
LKIVAFVKYS ALNPRESWDM WHPTLVAEAL FAIANIFSSL RLISLFTANS
510 520 530 540 550
HLGPLQISLG RMLLDILKFL FIYCLVLLAF ANGLNQLYFY YEETKGLSCK
560 570 580 590 600
GIRCEKQNNA FSTLFETLQS LFWSIFGLIN LYVTNVKAQH EFTEFVGATM
610 620 630 640 650
FGTYNVISLV VLLNMLIAMM NNSYQLIADH ADIEWKFART KLWMSYFEEG
660 670 680 690 700
GTLPTPFNVI PSPKSLWYLI KWIWTHLCKK KMRRKPESFG TIGRRAADNL
710 720 730 740 750
RRHHQYQEVM RNLVKRYVAA MIRDAKTEEG LTEENFKELK QDISSFRFEV
760 770 780 790 800
LGLLRGSKLS TVQSAQGTKE SSNSADSDEK SDNEGSSKDK KKNFSLFDLT
810 820 830 840 850
TLIHPRSAAI AAERHTISNG SALVVQEPPR EKQRKVNFVT DIRHFGLFHR
860 870 880 890 900
RSKQHAAAEQ NANQIFSVSE GVARQQAEGP LERSIQLESR TLASRGDLNI
910 920 930 940 950
PGLSEQCILV DHRERNTDSL GVQVSKRVCS FKSEKVVVED TVPIIPKEKK
960 970
HAKEEDSSAD YDANLTDTIT HEDYVTTRL
Length:979
Mass (Da):112,287
Last modified:May 3, 2011 - v2
Checksum:iE9365645BE640E09
GO
Isoform Beta (identifier: P79100-2) [UniParc]FASTAAdd to basket

Also known as: Delta 514

The sequence of this isoform differs from the canonical sequence as follows:
     1-511: Missing.

Show »
Length:468
Mass (Da):53,503
Checksum:i1B666EC6AC46A34F
GO

Sequence cautioni

The sequence CAA68125.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 511511Missing in isoform Beta. 1 PublicationVSP_006566Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99792 mRNA. Translation: CAA68125.1. Different initiation.
AJ224862 mRNA. Translation: CAA12161.1.
RefSeqiNP_776903.1. NM_174478.2.
XP_005213611.1. XM_005213554.3.
XP_010808964.1. XM_010810662.2.
XP_015329236.1. XM_015473750.1.
XP_015329237.1. XM_015473751.1.
XP_015329238.1. XM_015473752.1.
UniGeneiBt.4556.

Genome annotation databases

EnsembliENSBTAT00000065392; ENSBTAP00000055610; ENSBTAG00000009405. [P79100-2]
GeneIDi282102.
KEGGibta:282102.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99792 mRNA. Translation: CAA68125.1. Different initiation.
AJ224862 mRNA. Translation: CAA12161.1.
RefSeqiNP_776903.1. NM_174478.2.
XP_005213611.1. XM_005213554.3.
XP_010808964.1. XM_010810662.2.
XP_015329236.1. XM_015473750.1.
XP_015329237.1. XM_015473751.1.
XP_015329238.1. XM_015473752.1.
UniGeneiBt.4556.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012378.

Proteomic databases

PaxDbiP79100.
PRIDEiP79100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000065392; ENSBTAP00000055610; ENSBTAG00000009405. [P79100-2]
GeneIDi282102.
KEGGibta:282102.

Organism-specific databases

CTDi7223.

Phylogenomic databases

eggNOGiKOG3609. Eukaryota.
ENOG410XQ0Y. LUCA.
GeneTreeiENSGT00760000119180.
HOGENOMiHOG000020589.
HOVERGENiHBG068337.
InParanoidiP79100.
KOiK04967.
OrthoDBiEOG72G17P.
TreeFamiTF313147.

Enzyme and pathway databases

ReactomeiR-BTA-3295583. TRP channels.

Miscellaneous databases

NextBioi20805945.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005460. TRPC4_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERiPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF25. PTHR10117:SF25. 1 hit.
PfamiPF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSiPR01097. TRNSRECEPTRP.
PR01645. TRPCHANNEL4.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A mammalian capacitative calcium entry channel homologous to Drosophila TRP and TRPL."
    Philipp S., Cavalie A., Freichel M., Wissenbach U., Zimmer S., Trost C., Marquart A., Murakami M., Flockerzi V.
    EMBO J. 15:6166-6171(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
    Tissue: Adrenal gland and Retina.
  2. "Alternative splicing and tissue specific expression of the 5' truncated bCCE 1 variant bCCE 1delta514."
    Freichel M., Wissenbach U., Philipp S., Flockerzi V.
    FEBS Lett. 422:354-358(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
    Tissue: Adrenal gland.

Entry informationi

Entry nameiTRPC4_BOVIN
AccessioniPrimary (citable) accession number: P79100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 3, 2011
Last modified: May 11, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.