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P79098 (AMPN_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Aminopeptidase N

Short name=AP-N
Short name=bAPN
EC=3.4.11.2
Alternative name(s):
Alanyl aminopeptidase
Aminopeptidase M
Short name=AP-M
Microsomal aminopeptidase
CD_antigen=CD13
Gene names
Name:ANPEP
Synonyms:APN
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length965 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines and involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis By similarity. May serve as a receptor for bovine coronavirus (BCV) in a species-specific manner. Ref.2

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein.

Domain

Amino acids 647-837 are essential to mediate susceptibility to infection with TGEV (in human/bovine chimeric studies). Ref.2

Sequence similarities

Belongs to the peptidase M1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 965964Aminopeptidase N
PRO_0000095078

Regions

Topological domain2 – 87Cytoplasmic By similarity
Transmembrane9 – 3224Helical; Signal-anchor for type II membrane protein; Potential
Topological domain33 – 965933Extracellular By similarity
Region33 – 6533Cytosolic Ser/Thr-rich junction
Region66 – 965900Metalloprotease
Region349 – 3535Substrate binding By similarity

Sites

Active site3861Proton acceptor By similarity
Metal binding3851Zinc; catalytic By similarity
Metal binding3891Zinc; catalytic By similarity
Metal binding4081Zinc; catalytic By similarity
Site4741Transition state stabilizer By similarity

Amino acid modifications

Modified residue1731Sulfotyrosine Potential
Modified residue4161Sulfotyrosine Potential
Glycosylation1251N-linked (GlcNAc...) Potential
Glycosylation2311N-linked (GlcNAc...) Potential
Glycosylation2601N-linked (GlcNAc...) Potential
Glycosylation3161N-linked (GlcNAc...) Potential
Glycosylation5081N-linked (GlcNAc...) Potential
Glycosylation5691N-linked (GlcNAc...) Potential
Glycosylation6241N-linked (GlcNAc...) Potential
Glycosylation6801N-linked (GlcNAc...) Potential
Glycosylation7341N-linked (GlcNAc...) Potential
Glycosylation7381N-linked (GlcNAc...) Potential
Disulfide bond760 ↔ 767 By similarity
Disulfide bond797 ↔ 833 By similarity

Sequences

Sequence LengthMass (Da)Tools
P79098 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: C730910D173879A8

FASTA965109,276
        10         20         30         40         50         60 
MAKGFYISKA LGILAILLGV AAVATIIALS VVYAQEKNKN AERGTAAPTS PTGPTTTSAT 

        70         80         90        100        110        120 
TLDQSKPWNR YRLPTTLLPD SYRVTLRPYL TPNNNGLYIF TGSSTVRFTC KEPTDVIIIH 

       130        140        150        160        170        180 
SKKLNYTQHS GHLAALKGVG DTQAPEIDRT ELVLLTEYLV VHLKSSLEAG KTYEMETTFQ 

       190        200        210        220        230        240 
GELADDLAGF YRSEYMDGNV KKVLATTQMQ STDARKSFPC FDEPAMKATF NITLIHPKDL 

       250        260        270        280        290        300 
TALSNMPPKG PSVPFDGDSN WSVTEFETTP VMSTYLLAYI VSEFTSVESV APNDVQIRIW 

       310        320        330        340        350        360 
ARPKATADNH GLYALNVTGP ILNFFANHYN TAYPLPKSDQ IALPDFNAGA MENWGLVTYR 

       370        380        390        400        410        420 
ENALLYDPQS SSSSNKERVV TVIAHELAHQ WFGNLVTLAW WNDLWLNEGF ASYVEYLGAD 

       430        440        450        460        470        480 
YAEPTWNLKD LMVPNDVYSV MAVDALVTSH PLTTPANEVN TPAQISEMFD TISYSKGASV 

       490        500        510        520        530        540 
IRMLSNFLTE DLFKKGLASY LQTFAYQNTT YLNLWEHLQM AVENQLSIRL PDTVSAIMDR 

       550        560        570        580        590        600 
WTLQMGFPVI TVDTNTGTIS QKHFLLDPNS TVTRPSQFNY LWIVPISSIR NGQPQEHYWL 

       610        620        630        640        650        660 
RGEERNQNEL FKAAADDWVL LNINVTGYYQ VNYDENNWKK IQNQLMSRRE NIPVINRAQV 

       670        680        690        700        710        720 
IYDSFNLASA HMVPVTLALN NTLFLKNEME YMPWQAAVSS LNYFKLMFDR TEVYGPMQNY 

       730        740        750        760        770        780 
LKNQVEPIFL YFENLTKNWT EIPENLMDQY SEINAISTAC SNGLPKCEEL AKTLFNQWMN 

       790        800        810        820        830        840 
NPNVNPIDPN LRSTIYCNAI AQGGQEEWDF AWNQLQQAEL VNEADKLRSA LACTNHVWLL 

       850        860        870        880        890        900 
NRYLSYTLNP DLIRKQDATS TITSIASNVI GQSLAWDFIR SNWKKLFEDY GGGSFSFSNL 

       910        920        930        940        950        960 
IQGVTRRFST EFELQQLEEF KENNMDVGFG SGTRALEQAL EKTKANINWV KENKEVVLNW 


FKDHS 

« Hide

References

« Hide 'large scale' references
[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Ileum.
[2]"Interspecies aminopeptidase-N chimeras reveal species-specific receptor recognition by canine coronavirus, feline infectious peritonitis virus, and transmissible gastroenteritis virus."
Benbacer L., Kut E., Besnardeau L., Laude H., Delmas B.
J. Virol. 71:734-737(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 648-838, IDENTIFICATION OF RECEPTOR FUNCTIONAL DOMAINS FOR BCV INFECTION.
Tissue: Intestine.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC105142 mRNA. Translation: AAI05143.1.
X98240 mRNA. Translation: CAA66896.1.
RefSeqNP_001068612.1. NM_001075144.1.
UniGeneBt.27994.

3D structure databases

ProteinModelPortalP79098.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL2010632.

Protein family/group databases

MEROPSM01.001.

Proteomic databases

PRIDEP79098.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID404191.
KEGGbta:404191.

Organism-specific databases

CTD290.

Phylogenomic databases

eggNOGCOG0308.
HOGENOMHOG000106482.
HOVERGENHBG006616.
InParanoidP79098.
KOK11140.

Family and domain databases

InterProIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERPTHR11533. PTHR11533. 1 hit.
PfamPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSPR00756. ALADIPTASE.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20817619.

Entry information

Entry nameAMPN_BOVIN
AccessionPrimary (citable) accession number: P79098
Secondary accession number(s): Q3MHR1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 102 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries