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Protein

Aminopeptidase N

Gene

ANPEP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization.By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi385Zinc; catalyticPROSITE-ProRule annotation1
Active sitei386Proton acceptorPROSITE-ProRule annotation1
Metal bindingi389Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi408Zinc; catalyticPROSITE-ProRule annotation1
Sitei474Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Developmental protein, Host cell receptor for virus entry, Hydrolase, Metalloprotease, Protease, Receptor
Biological processAngiogenesis, Differentiation
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM01.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase NCurated (EC:3.4.11.2By similarity)
Short name:
AP-N
Short name:
bAPN
Alternative name(s):
Alanyl aminopeptidase
Aminopeptidase M
Short name:
AP-M
Microsomal aminopeptidase
CD_antigen: CD13
Gene namesi
Name:ANPEP
Synonyms:APN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicBy similarity8
Transmembranei9 – 32Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST24
Topological domaini33 – 965ExtracellularBy similarityAdd BLAST933

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2010632.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950781 – 965Aminopeptidase NAdd BLAST965

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi125N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei173SulfotyrosineSequence analysis1
Glycosylationi231N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi260N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi316N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei416SulfotyrosineSequence analysis1
Glycosylationi508N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi569N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi624N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi680N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi734N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi738N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi760 ↔ 767By similarity
Disulfide bondi797 ↔ 833By similarity

Post-translational modificationi

Sulfated.By similarity
N- and O-glycosylated.By similarity
May undergo proteolysis and give rise to a soluble form.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PaxDbiP79098.
PRIDEiP79098.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022456.

Structurei

3D structure databases

ProteinModelPortaliP79098.
SMRiP79098.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 65Cytosolic Ser/Thr-rich junctionAdd BLAST33
Regioni66 – 965MetalloproteaseAdd BLAST900
Regioni349 – 353Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
HOGENOMiHOG000106482.
HOVERGENiHBG006616.
InParanoidiP79098.
KOiK11140.

Family and domain databases

CDDicd09601. M1_APN_2. 1 hit.
InterProiView protein in InterPro
IPR024571. ERAP1-like_C_dom.
IPR034016. M1_APN-typ.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiView protein in Pfam
PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
PRINTSiPR00756. ALADIPTASE.
PROSITEiView protein in PROSITE
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P79098-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKGFYISKA LGILAILLGV AAVATIIALS VVYAQEKNKN AERGTAAPTS
60 70 80 90 100
PTGPTTTSAT TLDQSKPWNR YRLPTTLLPD SYRVTLRPYL TPNNNGLYIF
110 120 130 140 150
TGSSTVRFTC KEPTDVIIIH SKKLNYTQHS GHLAALKGVG DTQAPEIDRT
160 170 180 190 200
ELVLLTEYLV VHLKSSLEAG KTYEMETTFQ GELADDLAGF YRSEYMDGNV
210 220 230 240 250
KKVLATTQMQ STDARKSFPC FDEPAMKATF NITLIHPKDL TALSNMPPKG
260 270 280 290 300
PSVPFDGDSN WSVTEFETTP VMSTYLLAYI VSEFTSVESV APNDVQIRIW
310 320 330 340 350
ARPKATADNH GLYALNVTGP ILNFFANHYN TAYPLPKSDQ IALPDFNAGA
360 370 380 390 400
MENWGLVTYR ENALLYDPQS SSSSNKERVV TVIAHELAHQ WFGNLVTLAW
410 420 430 440 450
WNDLWLNEGF ASYVEYLGAD YAEPTWNLKD LMVPNDVYSV MAVDALVTSH
460 470 480 490 500
PLTTPANEVN TPAQISEMFD TISYSKGASV IRMLSNFLTE DLFKKGLASY
510 520 530 540 550
LQTFAYQNTT YLNLWEHLQM AVENQLSIRL PDTVSAIMDR WTLQMGFPVI
560 570 580 590 600
TVDTNTGTIS QKHFLLDPNS TVTRPSQFNY LWIVPISSIR NGQPQEHYWL
610 620 630 640 650
RGEERNQNEL FKAAADDWVL LNINVTGYYQ VNYDENNWKK IQNQLMSRRE
660 670 680 690 700
NIPVINRAQV IYDSFNLASA HMVPVTLALN NTLFLKNEME YMPWQAAVSS
710 720 730 740 750
LNYFKLMFDR TEVYGPMQNY LKNQVEPIFL YFENLTKNWT EIPENLMDQY
760 770 780 790 800
SEINAISTAC SNGLPKCEEL AKTLFNQWMN NPNVNPIDPN LRSTIYCNAI
810 820 830 840 850
AQGGQEEWDF AWNQLQQAEL VNEADKLRSA LACTNHVWLL NRYLSYTLNP
860 870 880 890 900
DLIRKQDATS TITSIASNVI GQSLAWDFIR SNWKKLFEDY GGGSFSFSNL
910 920 930 940 950
IQGVTRRFST EFELQQLEEF KENNMDVGFG SGTRALEQAL EKTKANINWV
960
KENKEVVLNW FKDHS
Length:965
Mass (Da):109,276
Last modified:January 23, 2007 - v4
Checksum:iC730910D173879A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105142 mRNA. Translation: AAI05143.1.
X98240 mRNA. Translation: CAA66896.1.
RefSeqiNP_001068612.1. NM_001075144.1.
UniGeneiBt.27994.

Genome annotation databases

GeneIDi404191.
KEGGibta:404191.

Entry informationi

Entry nameiAMPN_BOVIN
AccessioniPrimary (citable) accession number: P79098
Secondary accession number(s): Q3MHR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 119 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families