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P79098

- AMPN_BOVIN

UniProt

P79098 - AMPN_BOVIN

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Protein

Aminopeptidase N

Gene
ANPEP, APN
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines and involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis By similarity. May serve as a receptor for bovine coronavirus (BCV) in a species-specific manner.1 Publication

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi385 – 3851Zinc; catalytic By similarity
Active sitei386 – 3861Proton acceptor By similarity
Metal bindingi389 – 3891Zinc; catalytic By similarity
Metal bindingi408 – 4081Zinc; catalytic By similarity
Sitei474 – 4741Transition state stabilizer By similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. virus receptor activity Source: UniProtKB-KW
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. cell differentiation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Developmental protein, Host cell receptor for virus entry, Hydrolase, Metalloprotease, Protease, Receptor

Keywords - Biological processi

Angiogenesis, Differentiation

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Short name:
AP-N
Short name:
bAPN
Alternative name(s):
Alanyl aminopeptidase
Aminopeptidase M
Short name:
AP-M
Microsomal aminopeptidase
CD_antigen: CD13
Gene namesi
Name:ANPEP
Synonyms:APN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 87Cytoplasmic By similarity
Transmembranei9 – 3224Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini33 – 965933Extracellular By similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 965964Aminopeptidase NPRO_0000095078Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi125 – 1251N-linked (GlcNAc...) Reviewed prediction
Modified residuei173 – 1731Sulfotyrosine Reviewed prediction
Glycosylationi231 – 2311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi260 – 2601N-linked (GlcNAc...) Reviewed prediction
Glycosylationi316 – 3161N-linked (GlcNAc...) Reviewed prediction
Modified residuei416 – 4161Sulfotyrosine Reviewed prediction
Glycosylationi508 – 5081N-linked (GlcNAc...) Reviewed prediction
Glycosylationi569 – 5691N-linked (GlcNAc...) Reviewed prediction
Glycosylationi624 – 6241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi680 – 6801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi734 – 7341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi738 – 7381N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi760 ↔ 767 By similarity
Disulfide bondi797 ↔ 833 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PRIDEiP79098.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP79098.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 6533Cytosolic Ser/Thr-rich junctionAdd
BLAST
Regioni66 – 965900MetalloproteaseAdd
BLAST
Regioni349 – 3535Substrate binding By similarity

Domaini

Amino acids 647-837 are essential to mediate susceptibility to infection with TGEV (in human/bovine chimeric studies).1 Publication

Sequence similaritiesi

Belongs to the peptidase M1 family.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0308.
HOGENOMiHOG000106482.
HOVERGENiHBG006616.
InParanoidiP79098.
KOiK11140.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P79098-1 [UniParc]FASTAAdd to Basket

« Hide

MAKGFYISKA LGILAILLGV AAVATIIALS VVYAQEKNKN AERGTAAPTS    50
PTGPTTTSAT TLDQSKPWNR YRLPTTLLPD SYRVTLRPYL TPNNNGLYIF 100
TGSSTVRFTC KEPTDVIIIH SKKLNYTQHS GHLAALKGVG DTQAPEIDRT 150
ELVLLTEYLV VHLKSSLEAG KTYEMETTFQ GELADDLAGF YRSEYMDGNV 200
KKVLATTQMQ STDARKSFPC FDEPAMKATF NITLIHPKDL TALSNMPPKG 250
PSVPFDGDSN WSVTEFETTP VMSTYLLAYI VSEFTSVESV APNDVQIRIW 300
ARPKATADNH GLYALNVTGP ILNFFANHYN TAYPLPKSDQ IALPDFNAGA 350
MENWGLVTYR ENALLYDPQS SSSSNKERVV TVIAHELAHQ WFGNLVTLAW 400
WNDLWLNEGF ASYVEYLGAD YAEPTWNLKD LMVPNDVYSV MAVDALVTSH 450
PLTTPANEVN TPAQISEMFD TISYSKGASV IRMLSNFLTE DLFKKGLASY 500
LQTFAYQNTT YLNLWEHLQM AVENQLSIRL PDTVSAIMDR WTLQMGFPVI 550
TVDTNTGTIS QKHFLLDPNS TVTRPSQFNY LWIVPISSIR NGQPQEHYWL 600
RGEERNQNEL FKAAADDWVL LNINVTGYYQ VNYDENNWKK IQNQLMSRRE 650
NIPVINRAQV IYDSFNLASA HMVPVTLALN NTLFLKNEME YMPWQAAVSS 700
LNYFKLMFDR TEVYGPMQNY LKNQVEPIFL YFENLTKNWT EIPENLMDQY 750
SEINAISTAC SNGLPKCEEL AKTLFNQWMN NPNVNPIDPN LRSTIYCNAI 800
AQGGQEEWDF AWNQLQQAEL VNEADKLRSA LACTNHVWLL NRYLSYTLNP 850
DLIRKQDATS TITSIASNVI GQSLAWDFIR SNWKKLFEDY GGGSFSFSNL 900
IQGVTRRFST EFELQQLEEF KENNMDVGFG SGTRALEQAL EKTKANINWV 950
KENKEVVLNW FKDHS 965
Length:965
Mass (Da):109,276
Last modified:January 23, 2007 - v4
Checksum:iC730910D173879A8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC105142 mRNA. Translation: AAI05143.1.
X98240 mRNA. Translation: CAA66896.1.
RefSeqiNP_001068612.1. NM_001075144.1.
UniGeneiBt.27994.

Genome annotation databases

GeneIDi404191.
KEGGibta:404191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC105142 mRNA. Translation: AAI05143.1 .
X98240 mRNA. Translation: CAA66896.1 .
RefSeqi NP_001068612.1. NM_001075144.1.
UniGenei Bt.27994.

3D structure databases

ProteinModelPortali P79098.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL2010632.

Protein family/group databases

MEROPSi M01.001.

Proteomic databases

PRIDEi P79098.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 404191.
KEGGi bta:404191.

Organism-specific databases

CTDi 290.

Phylogenomic databases

eggNOGi COG0308.
HOGENOMi HOG000106482.
HOVERGENi HBG006616.
InParanoidi P79098.
KOi K11140.

Miscellaneous databases

NextBioi 20817619.

Family and domain databases

InterProi IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.
  2. "Interspecies aminopeptidase-N chimeras reveal species-specific receptor recognition by canine coronavirus, feline infectious peritonitis virus, and transmissible gastroenteritis virus."
    Benbacer L., Kut E., Besnardeau L., Laude H., Delmas B.
    J. Virol. 71:734-737(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 648-838, IDENTIFICATION OF RECEPTOR FUNCTIONAL DOMAINS FOR BCV INFECTION.
    Tissue: Intestine.

Entry informationi

Entry nameiAMPN_BOVIN
AccessioniPrimary (citable) accession number: P79098
Secondary accession number(s): Q3MHR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 102 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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