Reviewed, UniProtKB/Swiss-Prot P79089 (IDHP_ASPNG)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP], mitochondrial
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP

Gene names

Name:

icdA

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 498		Isocitrate dehydrogenase [NADP], mitochondrial		PRO_0000014423
Regions
	Nucleotide binding	164 – 166	3	NADP By similarity
	Nucleotide binding	397 – 402	6	NADP By similarity
	Region	183 – 189	7	Substrate binding By similarity
Sites
	Metal binding	339	1	Magnesium or manganese By similarity
	Metal binding	362	1	Magnesium or manganese By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	171	1	NADP By similarity
	Binding site	198	1	Substrate By similarity
	Binding site	221	1	Substrate By similarity
	Binding site	347	1	NADP By similarity
	Binding site	415	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	228	1	Critical for catalysis By similarity
	Site	299	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P79089-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 60FAB99B0A315CF0	FASTA	498	55,559
	10 20 30 40 50 60 MSSVRFSSAL ARRSFAVASP PLSAPLSSSA RRFLSSSSST ISSSSSSVST RSPRSLTSAS 70 80 90 100 110 120 SLLSSRTASA RWTGLSSLNL TQSRTMATEI PKIKVKNPVV ELDGDEMTRI IWQEIREKLI 130 140 150 160 170 180 LPYLDVDLKY YDLGLEYRDQ TDDQVTVEAA EAIKKYGVGV KCATITPDEA RVEEFKLKKM 190 200 210 220 230 240 WLSPNGTIRN ILGGTVFREP IIIPAIPRLV PGWNKPIIIG RHAFGDQYRA TDRVIPGPGK 250 260 270 280 290 300 LELVYTPVNG EPETVKVYDF QGGGIAQTQY NTDESIRGFA HASFQMALLK GLPLYMSTKN 310 320 330 340 350 360 TILKRYDGRF KDIFQEIYES TYQKDFEAKN LWYEHRLIDD MVAQMIKSEG GFVMALKNYD 370 380 390 400 410 420 GDVQSDIVAQ GFGSLGLMTS TLVTPTGEAF ESEAAHGTVT RHYREHQKGR ETSTNPIASI 430 440 450 460 470 480 FAWTRGLIQR GKLDETPDVV TFAEELERAC IEVVNDEGIM TKDLALACGR KEREAWVTTR 490 EYMAAVERRL KANLKSRL

References

[1]

"Cloning and expression of Aspergillus niger icdA gene encoding mitochondrial NADP+-specific isocitrate dehydrogenase." Kirimura K., Yoda M., Kumagai M., Ishii Y., Kino K., Usami S. J. Biosci. Bioeng. 93:136-144(2002) [PubMed: 16233178] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: WU-2223L.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB000262 Genomic DNA. Translation: BAA19074.1. AB000261 mRNA. Translation: BAA19073.1.
3D structure databases
HSSP	HSSP built from PDB template 1LWD based on UniProtKB P33198.
SMR	P79089. Positions 92-494.
ModBase	Search...
Family and domain databases
InterPro	IPR004790. IsoCit_DHase_NADP-dep_euk. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHP_ASPNG

Accession

Primary (citable) accession number: P79089

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)