Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acyl-CoA desaturase

Gene

Ole 1

Organism
Cryptococcus curvatus
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Stearyl-CoA desaturase that utilizes O2 and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates.UniRule annotation

Catalytic activityi

Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Expected to bind 2 Fe2+ ions per subunit.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotationImported

Keywords - Biological processi

Electron transportUniRule annotation, Fatty acid biosynthesisUniRule annotation, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport

Keywords - Ligandi

HemeUniRule annotationSAAS annotation, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-CoA desaturaseUniRule annotation (EC:1.14.19.1UniRule annotation)
Gene namesi
Name:Ole 1Imported
OrganismiCryptococcus curvatusImported
Taxonomic identifieri57679 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTrichosporonaceaeCutaneotrichosporon

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei77 – 9923HelicalSequence analysisAdd
BLAST
Transmembranei106 – 12722HelicalSequence analysisAdd
BLAST
Transmembranei139 – 15820HelicalSequence analysisAdd
BLAST
Transmembranei219 – 24022HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Structurei

3D structure databases

ProteinModelPortaliP79077.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini367 – 44983Cytochrome b5 heme-bindingInterPro annotationAdd
BLAST

Domaini

The histidine box domains are involved in binding the catalytic metal ions.UniRule annotation

Sequence similaritiesi

Belongs to the fatty acid desaturase type 1 family.UniRule annotation
Contains cytochrome b5 heme-binding domain.SAAS annotation

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysis

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR009160. Acyl-CoA_deSatase_haem/ster-bd.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR015876. Fatty_acid_desaturase-1_core.
IPR005804. Fatty_acid_desaturase_dom.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
[Graphical view]
PIRSFiPIRSF000345. OLE1. 1 hit.
PRINTSiPR00363. CYTOCHROMEB5.
PR00075. FACDDSATRASE.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P79077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASTATAPP ATAPAVANPT PAAASAAAAA PAATKDKAET IDPESEHFVV
60 70 80 90 100
SQNYVTRTVE NMTMLPPVTW SNLLQNIQWI SFTALTVPPA MAIYGLCTLE
110 120 130 140 150
LQRKTVIWAI VYYFMTGLGI TAGYHRLWAH RAYNASAPLQ YFLALCGAGS
160 170 180 190 200
VQGSIKWWSR GHRAHHRYTD TKLDPYSAHE GFWWAHVGWM LVKPRGKIGV
210 220 230 240 250
ADISDLSRNP VVKWQHNNYV MLMVLMGLVF PTLVAGLGWG DWKGGLLFAG
260 270 280 290 300
AARLVFVHHS TFCVNSLAHW LGETPFDNKH TPKDHFITAL VTVGEGYHNF
310 320 330 340 350
HHQFPMDFRN AIKWYQYDPT KWFIWTMSNV GLASHLKKFP DNEIKKGQYT
360 370 380 390 400
MKLQMLQEQS GSIQWPKHSN DLPVISWEDF QAEAKERSLV AIHGFIHDCS
410 420 430 440 450
SFLEDHPGGI HLIKKAIGTD ATTAFFGGVY DHSNAAHNLL AMMRVGILDG
460 470 480 490
GMEVESLKLE NLQRSMSVSS MESDAASSAS SVSVSSISPR WPH
Length:493
Mass (Da):54,718
Last modified:May 1, 1997 - v1
Checksum:i852620CC2CB32D1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10422 Genomic DNA. Translation: CAA71449.1.
PIRiS71634.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10422 Genomic DNA. Translation: CAA71449.1.
PIRiS71634.

3D structure databases

ProteinModelPortaliP79077.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR009160. Acyl-CoA_deSatase_haem/ster-bd.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR015876. Fatty_acid_desaturase-1_core.
IPR005804. Fatty_acid_desaturase_dom.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
[Graphical view]
PIRSFiPIRSF000345. OLE1. 1 hit.
PRINTSiPR00363. CYTOCHROMEB5.
PR00075. FACDDSATRASE.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of a delta-9 fatty acid desaturase gene from the oleaginous yeast Cryptococcus curvatus CBS 570."
    Meesters P.A., Eggink G.
    Yeast 12:723-730(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: CBS 570Imported.
  2. Springer J.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: CBS 570Imported.

Entry informationi

Entry nameiP79077_CRYCU
AccessioniPrimary (citable) accession number: P79077
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 1997
Last sequence update: May 1, 1997
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.