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Protein
Submitted name:

Endopolygalacturonase

Gene

cppg1

Organism
Chondrostereum purpureum
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei115 – 1151GalacturonateCombined sources
Binding sitei197 – 1971GalacturonateCombined sources
Binding sitei219 – 2191GalacturonateCombined sources
Binding sitei286 – 2861GalacturonateCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationSAAS annotationImported, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradationSAAS annotation

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.
mycoCLAPiPGA28A_CHOPU.

Names & Taxonomyi

Protein namesi
Submitted name:
EndopolygalacturonaseImported (EC:3.2.1.15Imported)
Gene namesi
Name:cppg1Imported
OrganismiChondrostereum purpureumImported
Taxonomic identifieri58369 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesSchizophyllaceaeChondrostereum

Subcellular locationi

  • Secreted SAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

SecretedSAAS annotation

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4249.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 403384Sequence analysisPRO_5007697358Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 41Combined sources
Glycosylationi116 – 1161N-linked (GlcNAc...)Combined sourcesCAR_5007364469
Glycosylationi185 – 1851N-linked (GlcNAc...)Combined sourcesCAR_5007364468
Disulfide bondi199 ↔ 215Combined sources
Disulfide bondi324 ↔ 327Combined sources

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5CX-ray0.96A25-359[»]
1KCCX-ray1.00A25-359[»]
1KCDX-ray1.15A25-359[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni174 – 1752Galacturonate bindingCombined sources
Regioni250 – 2523Galacturonate bindingCombined sources

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.UniRule annotationSAAS annotation

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P79074-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSFSSLFKL LILASATAAA PSKRATCTVK SVDDAKDIAG CSAVTLNGFT
60 70 80 90 100
VPAGNTLVLN PDKGATVTMA GDITFAKTTL DGPLFTIDGT GINFVGADHI
110 120 130 140 150
FDGNGALYWD GKGTNNGTHK PHPFLKIKGS GTYKKFEVLN SPAQAISVGP
160 170 180 190 200
TDAHLTLDGI TVDDFAGDTK NLGHNTDGFD VSANNVTIQN CIVKNQDDCI
210 220 230 240 250
AINDGNNIRF ENNQCSGGHG ISIGSIATGK HVSNVVIKGN TVTRSMYGVR
260 270 280 290 300
IKAQRTATSA SVSGVTYDAN TISGIAKYGV LISQSYPDDV GNPGTGAPFS
310 320 330 340 350
DVNFTGGATT IKVNNAATRV TVECGNCSGN WNWSQLTVTG GKAGTIKSDK
360 370 380 390 400
AKITGGQYLA DQPASNDIEE MPAQDPNDPE DPDTAMQEAE AEEAAAGNST

TSG
Length:403
Mass (Da):41,614
Last modified:May 1, 1997 - v1
Checksum:i7C43390AA5CB1F62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45072 mRNA. Translation: BAA08102.1.
AB043873 Genomic DNA. Translation: BAA96351.1.
PIRiJC5462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45072 mRNA. Translation: BAA08102.1.
AB043873 Genomic DNA. Translation: BAA96351.1.
PIRiJC5462.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5CX-ray0.96A25-359[»]
1KCCX-ray1.00A25-359[»]
1KCDX-ray1.15A25-359[»]
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL4249.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.
mycoCLAPiPGA28A_CHOPU.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence analysis of cDNA encoding endopolygalacturonase I from Stereum purpureum."
    Miyairi K., Senda M., Watanabe M., Okuno T.
    Biosci. Biotechnol. Biochem. 61:655-659(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ASP-4BImported.
  2. "Characterization of an endopolygalacturonase gene cppg1 from phytopathogenic fungus Chondrostereum purpureum."
    Senda M., Narita T., Akada S., Okuno T., Miyairi K.
    J. Gen. Plant Pathol. 67:41-44(2001)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ASP-4BImported.
  3. "Active-site architecture of endopolygalacturonase I from Stereum purpureum revealed by crystal structures in native and ligand-bound forms at atomic resolution."
    Shimizu T., Nakatsu T., Miyairi K., Okuno T., Kato H.
    Biochemistry 41:6651-6659(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (0.96 ANGSTROMS) OF 25-359 IN COMPLEX WITH GALACTURONATE, GLYCOSYLATION AT ASN-116 AND ASN-185, DISULFIDE BONDS.

Entry informationi

Entry nameiP79074_9AGAR
AccessioniPrimary (citable) accession number: P79074
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 1997
Last sequence update: May 1, 1997
Last modified: July 6, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.