P79074 (P79074_9AGAR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Endopolygalacturonase EMBL BAA08102.1
EC=3.2.1.15 EMBL BAA08102.1

Gene names

Name:

cppg1 EMBL BAA96351.1

Organism

Chondrostereum purpureum EMBL BAA08102.1

Taxonomic identifier

58369 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Schizophyllaceae › Chondrostereum

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the glycosyl hydrolase 28 family. RuleBase RU000404

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation SAAS SAAS000743 RuleBase RU000404
Cellular component	Secreted SAAS SAAS000743
Molecular function	Glycosidase SAAS SAAS000743 RuleBase RU000404 EMBL BAA08102.1 Hydrolase
Technical term	3D-structure PDB 1K5C PDB 1KCC PDB 1KCD
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	25 – 403	379	Potential EMBL BAA08102.1		PRO_5000139892
Amino acid modifications
	Glycosylation	116	1	N-linked (GlcNAc...) PDB 1K5C PDB 1KCC PDB 1KCD
	Glycosylation	185	1	N-linked (GlcNAc...) PDB 1K5C PDB 1KCC PDB 1KCD

Sequences

	Sequence		Length	Mass (Da)
	P79074 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 7C43390AA5CB1F62	FASTA	403	41,614
	10 20 30 40 50 60 MTSFSSLFKL LILASATAAA PSKRATCTVK SVDDAKDIAG CSAVTLNGFT VPAGNTLVLN 70 80 90 100 110 120 PDKGATVTMA GDITFAKTTL DGPLFTIDGT GINFVGADHI FDGNGALYWD GKGTNNGTHK 130 140 150 160 170 180 PHPFLKIKGS GTYKKFEVLN SPAQAISVGP TDAHLTLDGI TVDDFAGDTK NLGHNTDGFD 190 200 210 220 230 240 VSANNVTIQN CIVKNQDDCI AINDGNNIRF ENNQCSGGHG ISIGSIATGK HVSNVVIKGN 250 260 270 280 290 300 TVTRSMYGVR IKAQRTATSA SVSGVTYDAN TISGIAKYGV LISQSYPDDV GNPGTGAPFS 310 320 330 340 350 360 DVNFTGGATT IKVNNAATRV TVECGNCSGN WNWSQLTVTG GKAGTIKSDK AKITGGQYLA 370 380 390 400 DQPASNDIEE MPAQDPNDPE DPDTAMQEAE AEEAAAGNST TSG

References

[1]	"Characterization of an endopolygalacturonase gene cppg1 from phytopathogenic fungus Chondrostereum purpureum." Senda M., Narita T., Akada S., Okuno T., Miyairi K. J. Gen. Plant Pathol. 67:41-44(2001) Cited for: NUCLEOTIDE SEQUENCE. Strain: ASP-4B EMBL BAA96351.1.
[2]	"Cloning and sequence analysis of cDNA encoding endopolygalacturonase I from Stereum purpureum." Miyairi K., Senda M., Watanabe M., Hasui Y., Okuno T. Biosci. Biotechnol. Biochem. 61:655-659(1997) [PubMed: 9145525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ASP-4B EMBL BAA08102.1.
[3]	"Active-site architecture of endopolygalacturonase I from Stereum purpureum revealed by crystal structures in native and ligand-bound forms at atomic resolution." Shimizu T., Nakatsu T., Miyairi K., Okuno T., Kato H. Biochemistry 41:6651-6659(2002) [PubMed: 12022868] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.96 ANGSTROMS) OF 25-359, GLYCOSYLATION AT ASN-116 AND ASN-185.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D45072 mRNA. Translation: BAA08102.1.
AB043873 Genomic DNA. Translation: BAA96351.1.

PIR

JC5462.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5C	X-ray	0.96	A	25-359	[»]
1KCC	X-ray	1.00	A	25-359	[»]
1KCD	X-ray	1.15	A	25-359	[»]

ProteinModelPortal

P79074.

SMR

P79074. Positions 27-359.

ModBase

Search...

Protein family/group databases

CAZy

GH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]

Gene3D

G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.

Pfam

PF00295. Glyco_hydro_28. 1 hit.
[Graphical view]

SMART

SM00710. PbH1. 6 hits.
[Graphical view]

SUPFAM

SSF51126. Pectin_lyas_like. 1 hit.

PROSITE

PS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

P79074_9AGAR

Accession

Primary (citable) accession number: P79074

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 1997
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)