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P78985

- PFKA_ASPNG

UniProt

P78985 - PFKA_ASPNG

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA
Organism
Aspergillus niger
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231ATP; via amide nitrogen By similarity
Metal bindingi117 – 1171Magnesium; catalytic By similarity
Active sitei164 – 1641Proton acceptor By similarity
Binding sitei199 – 1991Substrate; shared with dimeric partner By similarity
Binding sitei263 – 2631Substrate By similarity
Binding sitei291 – 2911Substrate; shared with dimeric partner By similarity
Binding sitei480 – 4801Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei575 – 5751Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei642 – 6421Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei668 – 6681Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei749 – 7491Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 872ATP By similarity
Nucleotide bindingi116 – 1194ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP78985.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
OrganismiAspergillus niger
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 783783ATP-dependent 6-phosphofructokinaseUniRule annotation
PRO_0000112035Add
BLAST

Proteomic databases

PRIDEiP78985.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi5061.CADANGAP00013544.

Structurei

3D structure databases

ProteinModelPortaliP78985.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 389389N-terminal catalytic PFK domain 1UniRule annotation
Add
BLAST
Regioni162 – 1643Substrate binding By similarity
Regioni206 – 2083Substrate binding By similarity
Regioni297 – 3004Substrate binding By similarity
Regioni390 – 40314Interdomain linkerUniRule annotation
Add
BLAST
Regioni404 – 783380C-terminal regulatory PFK domain 2UniRule annotation
Add
BLAST
Regioni537 – 5415Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni582 – 5843Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni674 – 6774Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78985-1 [UniParc]FASTAAdd to Basket

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MAPPQAPVQP PKRRRIGVLT SGGDAPGMNG VVRAVVRMAI HSDCEAFAVY    50
EGYEGLVNGG DMIRQLHWED VRGWLSRGGT LIGSARCMTF RERPGRLRAA 100
KNMVLRGIDA LVVCGGDGSL TGADVFRSEW PGLLKELVET GELTEEQVKP 150
YQILNIVGLV GSIDNDMSGT DATIGCYSSL TRICDAVDDV FDTAFSHQRG 200
FVIEVMGRHC GWLALMSAIS TGADWLFVPE MPPKDGWEDD MCAIITKNRK 250
ERGKRRTIVI VAEGAQDRHL NKISSSKIKD ILTERLNLDT RVTVLGHTQR 300
GGAACAYDRW LSTLQGVEAV RAVLDMKPEA PSPVITIREN KILRMPLMDA 350
VQHTKTVTKH IQNKEFAEAM ALRDSEFKEY HFSYINTSTP DHPKLLLPEN 400
KRMRIGIIHV GAPAGGMNQA TRAAVAYCLT RGHTPLAIHN GFPGLCRHYD 450
DTPICSVREV AWQESDAWVN EGGSDIGTNR GLPGDDLATT AKSFKKFGFD 500
ALFVVGGFEA FTAVSQLRQA REKYPEFKIP MTVLPATISN NVPGTEYSLG 550
SDTCLNTLID FCDAIRQSAS SSRRRVFVIE TQGGKSGYIA TTAGLSVGAV 600
AVYIPEEGID IKMLARDIDF LRDNFARDKG ANRAGKIILR NECASSTYTT 650
QVVADMIKEE AKGRFESRAA VPGHFQQGGK PSPMDRIRAL RMATKCMLHL 700
ESYAGKSADE IAADELSASV IGIKGSQVLF SPMGGETGLE ATETDWARRR 750
PKTEFWLELQ DTVNILSGRA SVNNATWSCY ENA 783
Length:783
Mass (Da):85,760
Last modified:May 1, 1997 - v1
Checksum:i7BE3F6B553F06304
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z79690 Genomic DNA. Translation: CAB01923.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z79690 Genomic DNA. Translation: CAB01923.1 .

3D structure databases

ProteinModelPortali P78985.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5061.CADANGAP00013544.

Proteomic databases

PRIDEi P78985.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0205.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
SABIO-RK P78985.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. de Graaff L.H., Everse S.J., van den Broeck H.C., Bussink R., Visser J.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Entry informationi

Entry nameiPFKA_ASPNG
AccessioniPrimary (citable) accession number: P78985
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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