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P78974

- HEM2_SCHPO

UniProt

P78974 - HEM2_SCHPO

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Protein
Delta-aminolevulinic acid dehydratase
Gene
hem2, SPAC1805.06c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 1 zinc ion per monomer By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Zinc; catalytic By similarity
Metal bindingi124 – 1241Zinc; catalytic By similarity
Metal bindingi132 – 1321Zinc; catalytic By similarity
Active sitei199 – 1991Schiff-base intermediate with substrate By similarity
Binding sitei209 – 2091Substrate 1 By similarity
Binding sitei221 – 2211Substrate 1 By similarity
Active sitei252 – 2521Schiff-base intermediate with substrate By similarity
Binding sitei279 – 2791Substrate 2 By similarity
Binding sitei318 – 3181Substrate 2 By similarity

GO - Molecular functioni

  1. lead ion binding Source: UniProtKB
  2. porphobilinogen synthase activity Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. heme biosynthetic process Source: UniProtKB
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hem2
ORF Names:SPAC1805.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC1805.06c.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Delta-aminolevulinic acid dehydratase
PRO_0000140533Add
BLAST

Proteomic databases

MaxQBiP78974.
PaxDbiP78974.

Interactioni

Subunit structurei

Homooctamer By similarity.

Protein-protein interaction databases

BioGridi278837. 7 interactions.
MINTiMINT-4691801.
STRINGi4896.SPAC1805.06c-1.

Structurei

3D structure databases

ProteinModelPortaliP78974.
SMRiP78974. Positions 4-328.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALADH family.

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
KOiK01698.
OMAiVSRAMSY.
OrthoDBiEOG779P7W.
PhylomeDBiP78974.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78974-1 [UniParc]FASTAAdd to Basket

« Hide

MPVDISSLLH AGYSNPLLRE WQGVRPITKS SLMYPIFISE IDDAKEAIDS    50
MPNQFRWGVN RLEEFLGPLV KKGLRSVILF GVIESKKDYC GSMADSENGP 100
VIKAVKEIRH LFPELVVACD VCLCEYTDHG HCGLLYEDGT INNAKSVERI 150
AEVSGNYALA GAQIISPSDC MDGRVKAIKQ KLVELELSHK VCVISYSAKF 200
ASGFFGPFRA AANGAPKFGD RSCYQLPCNA RGLAKRAILR DVREGADGIM 250
VKPGTPYLDI LAMASKLADD LPIATYQVSG EFAIIHAAAA AGVFELKRHV 300
METMDGFMRA GANIVLTYFT PELLEWLEY 329
Length:329
Mass (Da):36,121
Last modified:December 1, 2000 - v2
Checksum:iA4BFA966C3C83F60
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601N → H in AAB41236. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAB55847.1.
U83568 mRNA. Translation: AAB41236.1.
PIRiT37891.
RefSeqiNP_593917.1. NM_001019346.2.

Genome annotation databases

EnsemblFungiiSPAC1805.06c.1; SPAC1805.06c.1:pep; SPAC1805.06c.
GeneIDi2542373.
KEGGispo:SPAC1805.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAB55847.1 .
U83568 mRNA. Translation: AAB41236.1 .
PIRi T37891.
RefSeqi NP_593917.1. NM_001019346.2.

3D structure databases

ProteinModelPortali P78974.
SMRi P78974. Positions 4-328.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 278837. 7 interactions.
MINTi MINT-4691801.
STRINGi 4896.SPAC1805.06c-1.

Proteomic databases

MaxQBi P78974.
PaxDbi P78974.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC1805.06c.1 ; SPAC1805.06c.1:pep ; SPAC1805.06c .
GeneIDi 2542373.
KEGGi spo:SPAC1805.06c.

Organism-specific databases

PomBasei SPAC1805.06c.

Phylogenomic databases

eggNOGi COG0113.
HOGENOMi HOG000020323.
KOi K01698.
OMAi VSRAMSY.
OrthoDBi EOG779P7W.
PhylomeDBi P78974.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Miscellaneous databases

NextBioi 20803432.
PROi P78974.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. Choi J.K., Levin H.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 60-329.

Entry informationi

Entry nameiHEM2_SCHPO
AccessioniPrimary (citable) accession number: P78974
Secondary accession number(s): Q9UTG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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