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Protein

WD repeat-containing protein slp1

Gene

slp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for mad2-dependent spindle checkpoint activation. Promotes ubiquitin-dependent degradation of cdc13 by the anaphase promoting complex/cyclosome (APC/C).3 Publications

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • cellular protein localization Source: PomBase
  • mitotic sister chromatid segregation Source: PomBase
  • positive regulation of ubiquitin protein ligase activity Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein slp1
Gene namesi
Name:slp1
ORF Names:SPAC821.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC821.08c.
PomBaseiSPAC821.08c. slp1.

Subcellular locationi

GO - Cellular componenti

  • cell division site Source: PomBase
  • mitotic checkpoint complex Source: PomBase
  • mitotic spindle Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi131 – 132AF → PY: Abrogates binding to mad2 and overrides activation of the spindle checkpoint by mad2. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512201 – 488WD repeat-containing protein slp1Add BLAST488

Proteomic databases

PRIDEiP78972.

Interactioni

Subunit structurei

Interacts with cdc13, mad3 and mes1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mad2O144173EBI-1252744,EBI-1269310
mes1P410052EBI-1252744,EBI-1553555

GO - Molecular functioni

  • anaphase-promoting complex binding Source: PomBase

Protein-protein interaction databases

BioGridi279650. 25 interactors.
DIPiDIP-38036N.
IntActiP78972. 4 interactors.
MINTiMINT-4691780.

Structurei

Secondary structure

1488
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi127 – 132Combined sources6
Beta strandi169 – 173Combined sources5
Beta strandi186 – 188Combined sources3
Beta strandi192 – 198Combined sources7
Beta strandi201 – 206Combined sources6
Turni207 – 209Combined sources3
Beta strandi212 – 217Combined sources6
Beta strandi224 – 229Combined sources6
Beta strandi233 – 240Combined sources8
Beta strandi245 – 249Combined sources5
Turni250 – 252Combined sources3
Beta strandi255 – 259Combined sources5
Beta strandi266 – 272Combined sources7
Beta strandi275 – 280Combined sources6
Beta strandi283 – 289Combined sources7
Beta strandi292 – 294Combined sources3
Beta strandi296 – 301Combined sources6
Beta strandi307 – 312Combined sources6
Beta strandi316 – 323Combined sources8
Beta strandi328 – 332Combined sources5
Beta strandi336 – 342Combined sources7
Beta strandi351 – 354Combined sources4
Beta strandi361 – 365Combined sources5
Turni368 – 370Combined sources3
Beta strandi372 – 377Combined sources6
Turni378 – 380Combined sources3
Beta strandi383 – 388Combined sources6
Beta strandi393 – 398Combined sources6
Beta strandi400 – 409Combined sources10
Turni411 – 413Combined sources3
Beta strandi415 – 422Combined sources8
Beta strandi425 – 433Combined sources9
Beta strandi441 – 444Combined sources4
Beta strandi448 – 454Combined sources7
Beta strandi458 – 464Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30A/D/G88-488[»]
ProteinModelPortaliP78972.
SMRiP78972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati178 – 215WD 1Add BLAST38
Repeati219 – 258WD 2Add BLAST40
Repeati261 – 298WD 3Add BLAST38
Repeati302 – 341WD 4Add BLAST40
Repeati344 – 386WD 5Add BLAST43
Repeati388 – 429WD 6Add BLAST42
Repeati434 – 473WD 7Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000195514.
KOiK03363.
OMAiEPRYREQ.
OrthoDBiEOG092C2B79.
PhylomeDBiP78972.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR033010. Cdc20/Fizzy.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19918. PTHR19918. 1 hit.
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIAGNSSTI SPTFSTPTKK RNLVFPNSPI TPLHQQALLG RNGRSSKRCS
60 70 80 90 100
PKSSFIRNSP KIDVVNTDWS IPLCGSPRNK SRPASRSDRF IPSRPNTANA
110 120 130 140 150
FVNSISSDVP FDYSESVAEA CGFDLNTRVL AFKLDAPEAK KPVDLRTQHN
160 170 180 190 200
RPQRPVVTPA KRRFNTTPER VLDAPGIIDD YYLNLLDWSN LNVVAVALER
210 220 230 240 250
NVYVWNADSG SVSALAETDE STYVASVKWS HDGSFLSVGL GNGLVDIYDV
260 270 280 290 300
ESQTKLRTMA GHQARVGCLS WNRHVLSSGS RSGAIHHHDV RIANHQIGTL
310 320 330 340 350
QGHSSEVCGL AWRSDGLQLA SGGNDNVVQI WDARSSIPKF TKTNHNAAVK
360 370 380 390 400
AVAWCPWQSN LLATGGGTMD KQIHFWNAAT GARVNTVDAG SQVTSLIWSP
410 420 430 440 450
HSKEIMSTHG FPDNNLSIWS YSSSGLTKQV DIPAHDTRVL YSALSPDGRI
460 470 480
LSTAASDENL KFWRVYDGDH VKRPIPITKT PSSSITIR
Length:488
Mass (Da):53,418
Last modified:May 1, 1997 - v1
Checksum:i52E021FD4EECE5DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77983 Genomic DNA. Translation: AAC49621.1.
CU329670 Genomic DNA. Translation: CAB57442.1.
PIRiT41719.
RefSeqiNP_593161.1. NM_001018559.2.

Genome annotation databases

EnsemblFungiiSPAC821.08c.1; SPAC821.08c.1:pep; SPAC821.08c.
GeneIDi2543222.
KEGGispo:SPAC821.08c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77983 Genomic DNA. Translation: AAC49621.1.
CU329670 Genomic DNA. Translation: CAB57442.1.
PIRiT41719.
RefSeqiNP_593161.1. NM_001018559.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30A/D/G88-488[»]
ProteinModelPortaliP78972.
SMRiP78972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279650. 25 interactors.
DIPiDIP-38036N.
IntActiP78972. 4 interactors.
MINTiMINT-4691780.

Proteomic databases

PRIDEiP78972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC821.08c.1; SPAC821.08c.1:pep; SPAC821.08c.
GeneIDi2543222.
KEGGispo:SPAC821.08c.

Organism-specific databases

EuPathDBiFungiDB:SPAC821.08c.
PomBaseiSPAC821.08c. slp1.

Phylogenomic databases

HOGENOMiHOG000195514.
KOiK03363.
OMAiEPRYREQ.
OrthoDBiEOG092C2B79.
PhylomeDBiP78972.

Miscellaneous databases

PROiP78972.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR033010. Cdc20/Fizzy.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19918. PTHR19918. 1 hit.
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSLP1_SCHPO
AccessioniPrimary (citable) accession number: P78972
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.