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P78972

- SLP1_SCHPO

UniProt

P78972 - SLP1_SCHPO

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Protein

WD repeat-containing protein slp1

Gene
slp1, SPAC821.08c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for mad2-dependent spindle checkpoint activation. Promotes ubiquitin-dependent degradation of cdc13 by the anaphase promoting complex/cyclosome (APC/C).3 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. activation of mitotic anaphase-promoting complex activity Source: PomBase
  2. cellular protein localization Source: PomBase
  3. mitotic sister chromatid segregation Source: PomBase
  4. mitotic spindle assembly checkpoint Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein slp1
Gene namesi
Name:slp1
ORF Names:SPAC821.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC821.08c.

Subcellular locationi

GO - Cellular componenti

  1. cell division site Source: PomBase
  2. condensed nuclear chromosome kinetochore Source: PomBase
  3. mitotic checkpoint complex Source: PomBase
  4. mitotic spindle pole body Source: PomBase
  5. nucleus Source: PomBase
  6. spindle Source: PomBase
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi131 – 1322AF → PY: Abrogates binding to mad2 and overrides activation of the spindle checkpoint by mad2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488WD repeat-containing protein slp1PRO_0000051220Add
BLAST

Interactioni

Subunit structurei

Interacts with cdc13, mad3 and mes1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mad2O144173EBI-1252744,EBI-1269310
mes1P410052EBI-1252744,EBI-1553555

Protein-protein interaction databases

BioGridi279650. 25 interactions.
DIPiDIP-38036N.
IntActiP78972. 4 interactions.
MINTiMINT-4691780.
STRINGi4896.SPAC821.08c-1.

Structurei

Secondary structure

1
488
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi127 – 1326
Beta strandi169 – 1735
Beta strandi186 – 1883
Beta strandi192 – 1987
Beta strandi201 – 2066
Turni207 – 2093
Beta strandi212 – 2176
Beta strandi224 – 2296
Beta strandi233 – 2408
Beta strandi245 – 2495
Turni250 – 2523
Beta strandi255 – 2595
Beta strandi266 – 2727
Beta strandi275 – 2806
Beta strandi283 – 2897
Beta strandi292 – 2943
Beta strandi296 – 3016
Beta strandi307 – 3126
Beta strandi316 – 3238
Beta strandi328 – 3325
Beta strandi336 – 3427
Beta strandi351 – 3544
Beta strandi361 – 3655
Turni368 – 3703
Beta strandi372 – 3776
Turni378 – 3803
Beta strandi383 – 3886
Beta strandi393 – 3986
Beta strandi400 – 40910
Turni411 – 4133
Beta strandi415 – 4228
Beta strandi425 – 4339
Beta strandi441 – 4444
Beta strandi448 – 4547
Beta strandi458 – 4647

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30A/D/G88-488[»]
ProteinModelPortaliP78972.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati178 – 21538WD 1Add
BLAST
Repeati219 – 25840WD 2Add
BLAST
Repeati261 – 29838WD 3Add
BLAST
Repeati302 – 34140WD 4Add
BLAST
Repeati344 – 38643WD 5Add
BLAST
Repeati388 – 42942WD 6Add
BLAST
Repeati434 – 47340WD 7Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
HOGENOMiHOG000195514.
KOiK03363.
OMAiHRTRIEY.
PhylomeDBiP78972.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78972-1 [UniParc]FASTAAdd to Basket

« Hide

MEIAGNSSTI SPTFSTPTKK RNLVFPNSPI TPLHQQALLG RNGRSSKRCS    50
PKSSFIRNSP KIDVVNTDWS IPLCGSPRNK SRPASRSDRF IPSRPNTANA 100
FVNSISSDVP FDYSESVAEA CGFDLNTRVL AFKLDAPEAK KPVDLRTQHN 150
RPQRPVVTPA KRRFNTTPER VLDAPGIIDD YYLNLLDWSN LNVVAVALER 200
NVYVWNADSG SVSALAETDE STYVASVKWS HDGSFLSVGL GNGLVDIYDV 250
ESQTKLRTMA GHQARVGCLS WNRHVLSSGS RSGAIHHHDV RIANHQIGTL 300
QGHSSEVCGL AWRSDGLQLA SGGNDNVVQI WDARSSIPKF TKTNHNAAVK 350
AVAWCPWQSN LLATGGGTMD KQIHFWNAAT GARVNTVDAG SQVTSLIWSP 400
HSKEIMSTHG FPDNNLSIWS YSSSGLTKQV DIPAHDTRVL YSALSPDGRI 450
LSTAASDENL KFWRVYDGDH VKRPIPITKT PSSSITIR 488
Length:488
Mass (Da):53,418
Last modified:May 1, 1997 - v1
Checksum:i52E021FD4EECE5DE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U77983 Genomic DNA. Translation: AAC49621.1.
CU329670 Genomic DNA. Translation: CAB57442.1.
PIRiT41719.
RefSeqiNP_593161.1. NM_001018559.2.

Genome annotation databases

EnsemblFungiiSPAC821.08c.1; SPAC821.08c.1:pep; SPAC821.08c.
GeneIDi2543222.
KEGGispo:SPAC821.08c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U77983 Genomic DNA. Translation: AAC49621.1 .
CU329670 Genomic DNA. Translation: CAB57442.1 .
PIRi T41719.
RefSeqi NP_593161.1. NM_001018559.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4AEZ X-ray 2.30 A/D/G 88-488 [» ]
ProteinModelPortali P78972.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279650. 25 interactions.
DIPi DIP-38036N.
IntActi P78972. 4 interactions.
MINTi MINT-4691780.
STRINGi 4896.SPAC821.08c-1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC821.08c.1 ; SPAC821.08c.1:pep ; SPAC821.08c .
GeneIDi 2543222.
KEGGi spo:SPAC821.08c.

Organism-specific databases

PomBasei SPAC821.08c.

Phylogenomic databases

eggNOGi COG2319.
HOGENOMi HOG000195514.
KOi K03363.
OMAi HRTRIEY.
PhylomeDBi P78972.

Miscellaneous databases

NextBioi 20804244.
PROi P78972.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 3 hits.
[Graphical view ]
SMARTi SM00320. WD40. 6 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A fission yeast homolog of CDC20/p55CDC/Fizzy is required for recovery from DNA damage and genetically interacts with p34cdc2."
    Matsumoto T.
    Mol. Cell. Biol. 17:742-750(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint."
    Kim S.H., Lin D.P., Matsumoto S., Kitazono A., Matsumoto T.
    Science 279:1045-1047(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF 131-ALA-PHE-132.
  4. "Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner."
    Millband D.N., Hardwick K.G.
    Mol. Cell. Biol. 22:2728-2742(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAD3.
  5. "Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p."
    Izawa D., Goto M., Yamashita A., Yamano H., Yamamoto M.
    Nature 434:529-533(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MES1 AND CDC13.

Entry informationi

Entry nameiSLP1_SCHPO
AccessioniPrimary (citable) accession number: P78972
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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