Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

WD repeat-containing protein slp1

Gene

slp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for mad2-dependent spindle checkpoint activation. Promotes ubiquitin-dependent degradation of cdc13 by the anaphase promoting complex/cyclosome (APC/C).3 Publications

GO - Biological processi

  1. activation of APC-Cdc20 complex activity Source: PomBase
  2. cell division Source: UniProtKB-KW
  3. cellular protein localization Source: PomBase
  4. mitotic sister chromatid segregation Source: PomBase
  5. mitotic spindle assembly checkpoint Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein slp1
Gene namesi
Name:slp1
ORF Names:SPAC821.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

PomBaseiSPAC821.08c.

Subcellular locationi

GO - Cellular componenti

  1. cell division site Source: PomBase
  2. condensed nuclear chromosome kinetochore Source: PomBase
  3. mitotic checkpoint complex Source: PomBase
  4. mitotic spindle Source: PomBase
  5. mitotic spindle pole body Source: PomBase
  6. nucleus Source: PomBase
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi131 – 1322AF → PY: Abrogates binding to mad2 and overrides activation of the spindle checkpoint by mad2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488WD repeat-containing protein slp1PRO_0000051220Add
BLAST

Interactioni

Subunit structurei

Interacts with cdc13, mad3 and mes1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mad2O144173EBI-1252744,EBI-1269310
mes1P410052EBI-1252744,EBI-1553555

Protein-protein interaction databases

BioGridi279650. 25 interactions.
DIPiDIP-38036N.
IntActiP78972. 4 interactions.
MINTiMINT-4691780.
STRINGi4896.SPAC821.08c-1.

Structurei

Secondary structure

1
488
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi127 – 1326Combined sources
Beta strandi169 – 1735Combined sources
Beta strandi186 – 1883Combined sources
Beta strandi192 – 1987Combined sources
Beta strandi201 – 2066Combined sources
Turni207 – 2093Combined sources
Beta strandi212 – 2176Combined sources
Beta strandi224 – 2296Combined sources
Beta strandi233 – 2408Combined sources
Beta strandi245 – 2495Combined sources
Turni250 – 2523Combined sources
Beta strandi255 – 2595Combined sources
Beta strandi266 – 2727Combined sources
Beta strandi275 – 2806Combined sources
Beta strandi283 – 2897Combined sources
Beta strandi292 – 2943Combined sources
Beta strandi296 – 3016Combined sources
Beta strandi307 – 3126Combined sources
Beta strandi316 – 3238Combined sources
Beta strandi328 – 3325Combined sources
Beta strandi336 – 3427Combined sources
Beta strandi351 – 3544Combined sources
Beta strandi361 – 3655Combined sources
Turni368 – 3703Combined sources
Beta strandi372 – 3776Combined sources
Turni378 – 3803Combined sources
Beta strandi383 – 3886Combined sources
Beta strandi393 – 3986Combined sources
Beta strandi400 – 40910Combined sources
Turni411 – 4133Combined sources
Beta strandi415 – 4228Combined sources
Beta strandi425 – 4339Combined sources
Beta strandi441 – 4444Combined sources
Beta strandi448 – 4547Combined sources
Beta strandi458 – 4647Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30A/D/G88-488[»]
ProteinModelPortaliP78972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati178 – 21538WD 1Add
BLAST
Repeati219 – 25840WD 2Add
BLAST
Repeati261 – 29838WD 3Add
BLAST
Repeati302 – 34140WD 4Add
BLAST
Repeati344 – 38643WD 5Add
BLAST
Repeati388 – 42942WD 6Add
BLAST
Repeati434 – 47340WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
HOGENOMiHOG000195514.
KOiK03363.
OMAiKEIMSTH.
PhylomeDBiP78972.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIAGNSSTI SPTFSTPTKK RNLVFPNSPI TPLHQQALLG RNGRSSKRCS
60 70 80 90 100
PKSSFIRNSP KIDVVNTDWS IPLCGSPRNK SRPASRSDRF IPSRPNTANA
110 120 130 140 150
FVNSISSDVP FDYSESVAEA CGFDLNTRVL AFKLDAPEAK KPVDLRTQHN
160 170 180 190 200
RPQRPVVTPA KRRFNTTPER VLDAPGIIDD YYLNLLDWSN LNVVAVALER
210 220 230 240 250
NVYVWNADSG SVSALAETDE STYVASVKWS HDGSFLSVGL GNGLVDIYDV
260 270 280 290 300
ESQTKLRTMA GHQARVGCLS WNRHVLSSGS RSGAIHHHDV RIANHQIGTL
310 320 330 340 350
QGHSSEVCGL AWRSDGLQLA SGGNDNVVQI WDARSSIPKF TKTNHNAAVK
360 370 380 390 400
AVAWCPWQSN LLATGGGTMD KQIHFWNAAT GARVNTVDAG SQVTSLIWSP
410 420 430 440 450
HSKEIMSTHG FPDNNLSIWS YSSSGLTKQV DIPAHDTRVL YSALSPDGRI
460 470 480
LSTAASDENL KFWRVYDGDH VKRPIPITKT PSSSITIR
Length:488
Mass (Da):53,418
Last modified:May 1, 1997 - v1
Checksum:i52E021FD4EECE5DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77983 Genomic DNA. Translation: AAC49621.1.
CU329670 Genomic DNA. Translation: CAB57442.1.
PIRiT41719.
RefSeqiNP_593161.1. NM_001018559.2.

Genome annotation databases

EnsemblFungiiSPAC821.08c.1; SPAC821.08c.1:pep; SPAC821.08c.
GeneIDi2543222.
KEGGispo:SPAC821.08c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77983 Genomic DNA. Translation: AAC49621.1.
CU329670 Genomic DNA. Translation: CAB57442.1.
PIRiT41719.
RefSeqiNP_593161.1. NM_001018559.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30A/D/G88-488[»]
ProteinModelPortaliP78972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279650. 25 interactions.
DIPiDIP-38036N.
IntActiP78972. 4 interactions.
MINTiMINT-4691780.
STRINGi4896.SPAC821.08c-1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC821.08c.1; SPAC821.08c.1:pep; SPAC821.08c.
GeneIDi2543222.
KEGGispo:SPAC821.08c.

Organism-specific databases

PomBaseiSPAC821.08c.

Phylogenomic databases

eggNOGiCOG2319.
HOGENOMiHOG000195514.
KOiK03363.
OMAiKEIMSTH.
PhylomeDBiP78972.

Miscellaneous databases

NextBioi20804244.
PROiP78972.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A fission yeast homolog of CDC20/p55CDC/Fizzy is required for recovery from DNA damage and genetically interacts with p34cdc2."
    Matsumoto T.
    Mol. Cell. Biol. 17:742-750(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint."
    Kim S.H., Lin D.P., Matsumoto S., Kitazono A., Matsumoto T.
    Science 279:1045-1047(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF 131-ALA-PHE-132.
  4. "Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner."
    Millband D.N., Hardwick K.G.
    Mol. Cell. Biol. 22:2728-2742(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAD3.
  5. "Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p."
    Izawa D., Goto M., Yamashita A., Yamano H., Yamamoto M.
    Nature 434:529-533(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MES1 AND CDC13.

Entry informationi

Entry nameiSLP1_SCHPO
AccessioniPrimary (citable) accession number: P78972
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: May 1, 1997
Last modified: March 4, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.