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P78972

- SLP1_SCHPO

UniProt

P78972 - SLP1_SCHPO

Protein

WD repeat-containing protein slp1

Gene

slp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Required for mad2-dependent spindle checkpoint activation. Promotes ubiquitin-dependent degradation of cdc13 by the anaphase promoting complex/cyclosome (APC/C).3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. activation of mitotic anaphase-promoting complex activity Source: PomBase
    2. cellular protein localization Source: PomBase
    3. mitotic sister chromatid segregation Source: PomBase
    4. mitotic spindle assembly checkpoint Source: PomBase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    WD repeat-containing protein slp1
    Gene namesi
    Name:slp1
    ORF Names:SPAC821.08c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC821.08c.

    Subcellular locationi

    GO - Cellular componenti

    1. cell division site Source: PomBase
    2. condensed nuclear chromosome kinetochore Source: PomBase
    3. mitotic checkpoint complex Source: PomBase
    4. mitotic spindle pole body Source: PomBase
    5. nucleus Source: PomBase
    6. spindle Source: PomBase

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi131 – 1322AF → PY: Abrogates binding to mad2 and overrides activation of the spindle checkpoint by mad2. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488WD repeat-containing protein slp1PRO_0000051220Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with cdc13, mad3 and mes1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    mad2O144173EBI-1252744,EBI-1269310
    mes1P410052EBI-1252744,EBI-1553555

    Protein-protein interaction databases

    BioGridi279650. 25 interactions.
    DIPiDIP-38036N.
    IntActiP78972. 4 interactions.
    MINTiMINT-4691780.
    STRINGi4896.SPAC821.08c-1.

    Structurei

    Secondary structure

    1
    488
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi127 – 1326
    Beta strandi169 – 1735
    Beta strandi186 – 1883
    Beta strandi192 – 1987
    Beta strandi201 – 2066
    Turni207 – 2093
    Beta strandi212 – 2176
    Beta strandi224 – 2296
    Beta strandi233 – 2408
    Beta strandi245 – 2495
    Turni250 – 2523
    Beta strandi255 – 2595
    Beta strandi266 – 2727
    Beta strandi275 – 2806
    Beta strandi283 – 2897
    Beta strandi292 – 2943
    Beta strandi296 – 3016
    Beta strandi307 – 3126
    Beta strandi316 – 3238
    Beta strandi328 – 3325
    Beta strandi336 – 3427
    Beta strandi351 – 3544
    Beta strandi361 – 3655
    Turni368 – 3703
    Beta strandi372 – 3776
    Turni378 – 3803
    Beta strandi383 – 3886
    Beta strandi393 – 3986
    Beta strandi400 – 40910
    Turni411 – 4133
    Beta strandi415 – 4228
    Beta strandi425 – 4339
    Beta strandi441 – 4444
    Beta strandi448 – 4547
    Beta strandi458 – 4647

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4AEZX-ray2.30A/D/G88-488[»]
    ProteinModelPortaliP78972.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati178 – 21538WD 1Add
    BLAST
    Repeati219 – 25840WD 2Add
    BLAST
    Repeati261 – 29838WD 3Add
    BLAST
    Repeati302 – 34140WD 4Add
    BLAST
    Repeati344 – 38643WD 5Add
    BLAST
    Repeati388 – 42942WD 6Add
    BLAST
    Repeati434 – 47340WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat CDC20/Fizzy family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000195514.
    KOiK03363.
    OMAiHRTRIEY.
    PhylomeDBiP78972.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 3 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 6 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P78972-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEIAGNSSTI SPTFSTPTKK RNLVFPNSPI TPLHQQALLG RNGRSSKRCS    50
    PKSSFIRNSP KIDVVNTDWS IPLCGSPRNK SRPASRSDRF IPSRPNTANA 100
    FVNSISSDVP FDYSESVAEA CGFDLNTRVL AFKLDAPEAK KPVDLRTQHN 150
    RPQRPVVTPA KRRFNTTPER VLDAPGIIDD YYLNLLDWSN LNVVAVALER 200
    NVYVWNADSG SVSALAETDE STYVASVKWS HDGSFLSVGL GNGLVDIYDV 250
    ESQTKLRTMA GHQARVGCLS WNRHVLSSGS RSGAIHHHDV RIANHQIGTL 300
    QGHSSEVCGL AWRSDGLQLA SGGNDNVVQI WDARSSIPKF TKTNHNAAVK 350
    AVAWCPWQSN LLATGGGTMD KQIHFWNAAT GARVNTVDAG SQVTSLIWSP 400
    HSKEIMSTHG FPDNNLSIWS YSSSGLTKQV DIPAHDTRVL YSALSPDGRI 450
    LSTAASDENL KFWRVYDGDH VKRPIPITKT PSSSITIR 488
    Length:488
    Mass (Da):53,418
    Last modified:May 1, 1997 - v1
    Checksum:i52E021FD4EECE5DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77983 Genomic DNA. Translation: AAC49621.1.
    CU329670 Genomic DNA. Translation: CAB57442.1.
    PIRiT41719.
    RefSeqiNP_593161.1. NM_001018559.2.

    Genome annotation databases

    EnsemblFungiiSPAC821.08c.1; SPAC821.08c.1:pep; SPAC821.08c.
    GeneIDi2543222.
    KEGGispo:SPAC821.08c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77983 Genomic DNA. Translation: AAC49621.1 .
    CU329670 Genomic DNA. Translation: CAB57442.1 .
    PIRi T41719.
    RefSeqi NP_593161.1. NM_001018559.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4AEZ X-ray 2.30 A/D/G 88-488 [» ]
    ProteinModelPortali P78972.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279650. 25 interactions.
    DIPi DIP-38036N.
    IntActi P78972. 4 interactions.
    MINTi MINT-4691780.
    STRINGi 4896.SPAC821.08c-1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC821.08c.1 ; SPAC821.08c.1:pep ; SPAC821.08c .
    GeneIDi 2543222.
    KEGGi spo:SPAC821.08c.

    Organism-specific databases

    PomBasei SPAC821.08c.

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000195514.
    KOi K03363.
    OMAi HRTRIEY.
    PhylomeDBi P78972.

    Miscellaneous databases

    NextBioi 20804244.
    PROi P78972.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 3 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 6 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A fission yeast homolog of CDC20/p55CDC/Fizzy is required for recovery from DNA damage and genetically interacts with p34cdc2."
      Matsumoto T.
      Mol. Cell. Biol. 17:742-750(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint."
      Kim S.H., Lin D.P., Matsumoto S., Kitazono A., Matsumoto T.
      Science 279:1045-1047(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF 131-ALA-PHE-132.
    4. "Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner."
      Millband D.N., Hardwick K.G.
      Mol. Cell. Biol. 22:2728-2742(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAD3.
    5. "Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p."
      Izawa D., Goto M., Yamashita A., Yamano H., Yamamoto M.
      Nature 434:529-533(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MES1 AND CDC13.

    Entry informationi

    Entry nameiSLP1_SCHPO
    AccessioniPrimary (citable) accession number: P78972
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2002
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3