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P78972 (SLP1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
WD repeat-containing protein slp1
Gene names
Name:slp1
ORF Names:SPAC821.08c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length488 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for mad2-dependent spindle checkpoint activation. Promotes ubiquitin-dependent degradation of cdc13 by the anaphase promoting complex/cyclosome (APC/C). Ref.1 Ref.3 Ref.5

Subunit structure

Interacts with cdc13, mad3 and mes1. Ref.4 Ref.5

Sequence similarities

Belongs to the WD repeat CDC20/Fizzy family.

Contains 7 WD repeats.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 488488WD repeat-containing protein slp1
PRO_0000051220

Regions

Repeat178 – 21538WD 1
Repeat219 – 25840WD 2
Repeat261 – 29838WD 3
Repeat302 – 34140WD 4
Repeat344 – 38643WD 5
Repeat388 – 42942WD 6
Repeat434 – 47340WD 7

Experimental info

Mutagenesis131 – 1322AF → PY: Abrogates binding to mad2 and overrides activation of the spindle checkpoint by mad2. Ref.3

Secondary structure

.................................................................... 488
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P78972 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 52E021FD4EECE5DE

FASTA48853,418
        10         20         30         40         50         60 
MEIAGNSSTI SPTFSTPTKK RNLVFPNSPI TPLHQQALLG RNGRSSKRCS PKSSFIRNSP 

        70         80         90        100        110        120 
KIDVVNTDWS IPLCGSPRNK SRPASRSDRF IPSRPNTANA FVNSISSDVP FDYSESVAEA 

       130        140        150        160        170        180 
CGFDLNTRVL AFKLDAPEAK KPVDLRTQHN RPQRPVVTPA KRRFNTTPER VLDAPGIIDD 

       190        200        210        220        230        240 
YYLNLLDWSN LNVVAVALER NVYVWNADSG SVSALAETDE STYVASVKWS HDGSFLSVGL 

       250        260        270        280        290        300 
GNGLVDIYDV ESQTKLRTMA GHQARVGCLS WNRHVLSSGS RSGAIHHHDV RIANHQIGTL 

       310        320        330        340        350        360 
QGHSSEVCGL AWRSDGLQLA SGGNDNVVQI WDARSSIPKF TKTNHNAAVK AVAWCPWQSN 

       370        380        390        400        410        420 
LLATGGGTMD KQIHFWNAAT GARVNTVDAG SQVTSLIWSP HSKEIMSTHG FPDNNLSIWS 

       430        440        450        460        470        480 
YSSSGLTKQV DIPAHDTRVL YSALSPDGRI LSTAASDENL KFWRVYDGDH VKRPIPITKT 


PSSSITIR 

« Hide

References

« Hide 'large scale' references
[1]"A fission yeast homolog of CDC20/p55CDC/Fizzy is required for recovery from DNA damage and genetically interacts with p34cdc2."
Matsumoto T.
Mol. Cell. Biol. 17:742-750(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: 972 / ATCC 24843.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint."
Kim S.H., Lin D.P., Matsumoto S., Kitazono A., Matsumoto T.
Science 279:1045-1047(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF 131-ALA-PHE-132.
[4]"Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner."
Millband D.N., Hardwick K.G.
Mol. Cell. Biol. 22:2728-2742(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAD3.
[5]"Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p."
Izawa D., Goto M., Yamashita A., Yamano H., Yamamoto M.
Nature 434:529-533(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MES1 AND CDC13.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U77983 Genomic DNA. Translation: AAC49621.1.
CU329670 Genomic DNA. Translation: CAB57442.1.
PIRT41719.
RefSeqNP_593161.1. NM_001018559.2.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30A/D/G88-488[»]
ProteinModelPortalP78972.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid279650. 25 interactions.
DIPDIP-38036N.
IntActP78972. 4 interactions.
MINTMINT-4691780.
STRING4896.SPAC821.08c-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC821.08c.1; SPAC821.08c.1:pep; SPAC821.08c.
GeneID2543222.
KEGGspo:SPAC821.08c.

Organism-specific databases

PomBaseSPAC821.08c.

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000195514.
KOK03363.
OMAHRTRIEY.
PhylomeDBP78972.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 3 hits.
[Graphical view]
SMARTSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20804244.
PROP78972.

Entry information

Entry nameSLP1_SCHPO
AccessionPrimary (citable) accession number: P78972
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references