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Protein

Sphingosine N-acyltransferase lag1

Gene

lag1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ceramide synthase complex required for C26-CoA-dependent ceramide synthesis. Facilitates ER-to-Golgi transport of GPI-anchored proteins (By similarity). Involved in the aging process (By similarity).By similarity

Catalytic activityi

Acyl-CoA + sphingosine = CoA + N-acylsphingosine.

GO - Molecular functioni

GO - Biological processi

  • ceramide biosynthetic process Source: PomBase
  • vacuolar acidification Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-SPO-1660661. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine N-acyltransferase lag1 (EC:2.3.1.24)
Alternative name(s):
Longevity assurance factor 1
Longevity assurance protein 1
Gene namesi
Name:lag1
ORF Names:SPAC1A6.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1A6.09c.
PomBaseiSPAC1A6.09c. lag1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei63 – 83HelicalSequence analysisAdd BLAST21
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21
Transmembranei231 – 251HelicalSequence analysisAdd BLAST21
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001855261 – 390Sphingosine N-acyltransferase lag1Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi26N-linked (GlcNAc...)Sequence analysis1
Modified residuei372Phosphoserine1 Publication1
Modified residuei374Phosphoserine1 Publication1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP78970.
PRIDEiP78970.

PTM databases

iPTMnetiP78970.

Interactioni

Protein-protein interaction databases

BioGridi278925. 34 interactors.
MINTiMINT-4691767.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 365TLCPROSITE-ProRule annotationAdd BLAST213

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 16Poly-Ser4

Sequence similaritiesi

Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiP78970.
KOiK04709.
OMAiGAHIMAT.
OrthoDBiEOG092C45Y7.
PhylomeDBiP78970.

Family and domain databases

InterProiIPR016439. Lag1/Lac1.
IPR006634. TLC-dom.
IPR013599. TRAM1.
[Graphical view]
PANTHERiPTHR12560. PTHR12560. 1 hit.
PfamiPF08390. TRAM1. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78970-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNRKADEKH HMSSSSLTND RSYIRNLSNR KTSISRKVPI TRTLEDPSNF
60 70 80 90 100
VAKDGTKLVQ APLFLLVWQK EICLSIIAIC FACLLSPSLR PYAEPFIFLS
110 120 130 140 150
YKQPDGSYGK GPKDACFPIF WVIVFTAFRV IVMDYVFRPF VLNWGVRNRK
160 170 180 190 200
VIIRFCEQGY SFFYYLCFWF LGLYIYRSSN YWSNEEKLFE DYPQYYMSPL
210 220 230 240 250
FKAYYLIQLG FWLQQILVLH LEQRRADHWQ MFAHHIVTCA LIILSYGFNF
260 270 280 290 300
LRVGNAILYI FDLSDYILSG GKMLKYLGFG KICDYLFGIF VASWVYSRHY
310 320 330 340 350
LFSKILRVVV TNAPEIIGGF HLDVPNGYIF NKPIYIAFII LLFTLQLLIY
360 370 380 390
IWFGMIVKVA YRVFSGEEAT DSRSDDEGED EEASSTNEDK
Length:390
Mass (Da):45,668
Last modified:December 15, 1998 - v2
Checksum:iDC00FB5C2D2F22CC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti379 – 390EDEEA…TNEDK → GRRGGEFNE in AAB19113 (Ref. 1) CuratedAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76608 Genomic DNA. Translation: AAB19113.1.
CU329670 Genomic DNA. Translation: CAB16359.1.
PIRiT38012.
RefSeqiNP_593201.1. NM_001018597.2.

Genome annotation databases

EnsemblFungiiSPAC1A6.09c.1; SPAC1A6.09c.1:pep; SPAC1A6.09c.
GeneIDi2542464.
KEGGispo:SPAC1A6.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76608 Genomic DNA. Translation: AAB19113.1.
CU329670 Genomic DNA. Translation: CAB16359.1.
PIRiT38012.
RefSeqiNP_593201.1. NM_001018597.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278925. 34 interactors.
MINTiMINT-4691767.

PTM databases

iPTMnetiP78970.

Proteomic databases

MaxQBiP78970.
PRIDEiP78970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1A6.09c.1; SPAC1A6.09c.1:pep; SPAC1A6.09c.
GeneIDi2542464.
KEGGispo:SPAC1A6.09c.

Organism-specific databases

EuPathDBiFungiDB:SPAC1A6.09c.
PomBaseiSPAC1A6.09c. lag1.

Phylogenomic databases

InParanoidiP78970.
KOiK04709.
OMAiGAHIMAT.
OrthoDBiEOG092C45Y7.
PhylomeDBiP78970.

Enzyme and pathway databases

ReactomeiR-SPO-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

PROiP78970.

Family and domain databases

InterProiIPR016439. Lag1/Lac1.
IPR006634. TLC-dom.
IPR013599. TRAM1.
[Graphical view]
PANTHERiPTHR12560. PTHR12560. 1 hit.
PfamiPF08390. TRAM1. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLAG1_SCHPO
AccessioniPrimary (citable) accession number: P78970
Secondary accession number(s): O13860
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: November 30, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.