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Protein

Protein arginine N-methyltransferase skb1

Gene

skb1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that can catalyze both the mono- and symmetric (type II) dimethylation of the guanidino nitrogens of arginine residues in target proteins (By similarity). Positively modulates the shk1 kinase function. May be a mediator of hyperosmotic stress response (PubMed:11278267).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei316 – 3161S-adenosyl-L-methionineBy similarity
Sitei319 – 3191Critical for specifying symmetric addition of methyl groupsBy similarity
Binding sitei379 – 3791S-adenosyl-L-methionineBy similarity
Active sitei422 – 4221Proton donor/acceptorBy similarity
Active sitei431 – 4311Proton donor/acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellular protein localization Source: PomBase
  • cellular response to osmotic stress Source: PomBase
  • establishment or maintenance of bipolar cell polarity regulating cell shape Source: PomBase
  • lateral cortical node assembly Source: PomBase
  • peptidyl-arginine N-methylation Source: PomBase
  • protein localization to lateral cortical node Source: PomBase
  • regulation of establishment or maintenance of bipolar cell polarity regulating cell shape Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein arginine N-methyltransferase skb11 Publication (EC:2.1.1.-By similarity)
Alternative name(s):
Shk1 kinase-binding protein 11 Publication
Type II protein arginine N-methyltransferaseBy similarity
Short name:
Type II PRMTBy similarity
Gene namesi
Name:skb11 Publication
Synonyms:rmt5Imported
ORF Names:SPBC16H5.11cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC16H5.11c.
PomBaseiSPBC16H5.11c. skb1.

Subcellular locationi

GO - Cellular componenti

  • Cdc42 GTPase complex Source: PomBase
  • cell cortex Source: PomBase
  • cell tip Source: PomBase
  • lateral cortical node Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 645645Protein arginine N-methyltransferase skb1PRO_0000212346Add
BLAST

Proteomic databases

MaxQBiP78963.

Interactioni

Subunit structurei

Interacts with the N-terminal regulatory domain of shk1. Shk1, cdc42 and skb1 are able to form a ternary complex in vivo.1 Publication

Protein-protein interaction databases

BioGridi276637. 37 interactions.
IntActiP78963. 1 interaction.
MINTiMINT-4691688.

Structurei

3D structure databases

ProteinModelPortaliP78963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini300 – 608309SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni325 – 3262S-adenosyl-L-methionine bindingBy similarity
Regioni406 – 4072S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase PRMT-type domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000175933.
InParanoidiP78963.
KOiK02516.
OMAiCANAYLV.
OrthoDBiEOG7KSXMV.
PhylomeDBiP78963.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025799. Arg_MeTrfase.
IPR007857. Arg_MeTrfase_PRMT5.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10738. PTHR10738. 1 hit.
PfamiPF05185. PRMT5. 1 hit.
[Graphical view]
PIRSFiPIRSF015894. Skb1_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51678. SAM_MT_PRMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRDGRIPQ YSIYPLPVPP MNSKTPTLGI VCSEGTISLS LEEGFEFVGV
60 70 80 90 100
PLSGEGLKLR VEALAPSERL QEFLDDEVAY HPEENVHKVV GLSSAWLELD
110 120 130 140 150
SEDTLIADRS EEVLLKEASY ASYCGLSSII LNGPTSPMNV MRYARAVSSA
160 170 180 190 200
LNSTMNLKFL VQLAIESGHE DYFETWKMWD TIRSACGYHP RLKVALELPP
210 220 230 240 250
ACSPPIELVN RWYAEPIEMI TMSCMAFVPN PNGYPVLGRK LRAIYALYLR
260 270 280 290 300
LNPRILLWDN DAPEKIGDSP DYSIYMKHLF DSQPPAPLVE DLADSYKDYL
310 320 330 340 350
QVPLQPLSYN LENITYEIFE RDPVKYAQYE QAIFSALMDR DESSVTRIAV
360 370 380 390 400
VGAGRGPLVD CALRAAISSS RTVDMIALEK NPNAFSMLLM RNRQDWAGKV
410 420 430 440 450
TLVFGDMRTW NPDYKIDILV SELLGSMGDN ELSPECLDGV QHVLDEETGI
460 470 480 490 500
CIPSSYISYV TPIMSPKLWS EARNMNDPNA FERQYVVLMN SFDFLAADDE
510 520 530 540 550
FRFQSLWSFH HPNKDSEVYT KNLHNKRFAS VRFQASSPGI LHGFAGYFEA
560 570 580 590 600
TLYKDISLSI MPATMEAKSP DMFSWFPIYM PIKKPMYVPE NSQLEFHMWR
610 620 630 640
LTDGMRVWFE WCANAYLVLR NGSQIKLSST EVHNISGKAF SCNMY
Length:645
Mass (Da):73,167
Last modified:January 11, 2001 - v2
Checksum:i288FEE7BDF61558D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451F → L in AAC49571 (PubMed:8943016).Curated
Sequence conflicti134 – 1385PTSPM → SNFTQC in AAC49571 (PubMed:8943016).Curated
Sequence conflicti195 – 1951A → G in AAC49571 (PubMed:8943016).Curated
Sequence conflicti398 – 3981G → A in AAC49571 (PubMed:8943016).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59684 Genomic DNA. Translation: AAC49571.1.
CU329671 Genomic DNA. Translation: CAA17909.1.
PIRiT39614.
T48644.
RefSeqiNP_595936.1. NM_001021844.2.

Genome annotation databases

EnsemblFungiiSPBC16H5.11c.1; SPBC16H5.11c.1:pep; SPBC16H5.11c.
GeneIDi2540099.
KEGGispo:SPBC16H5.11c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59684 Genomic DNA. Translation: AAC49571.1.
CU329671 Genomic DNA. Translation: CAA17909.1.
PIRiT39614.
T48644.
RefSeqiNP_595936.1. NM_001021844.2.

3D structure databases

ProteinModelPortaliP78963.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276637. 37 interactions.
IntActiP78963. 1 interaction.
MINTiMINT-4691688.

Proteomic databases

MaxQBiP78963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC16H5.11c.1; SPBC16H5.11c.1:pep; SPBC16H5.11c.
GeneIDi2540099.
KEGGispo:SPBC16H5.11c.

Organism-specific databases

EuPathDBiFungiDB:SPBC16H5.11c.
PomBaseiSPBC16H5.11c. skb1.

Phylogenomic databases

HOGENOMiHOG000175933.
InParanoidiP78963.
KOiK02516.
OMAiCANAYLV.
OrthoDBiEOG7KSXMV.
PhylomeDBiP78963.

Miscellaneous databases

PROiP78963.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025799. Arg_MeTrfase.
IPR007857. Arg_MeTrfase_PRMT5.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10738. PTHR10738. 1 hit.
PfamiPF05185. PRMT5. 1 hit.
[Graphical view]
PIRSFiPIRSF015894. Skb1_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51678. SAM_MT_PRMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The highly conserved skb1 gene encodes a protein that interacts with Shk1, a fission yeast Ste20/PAK homolog."
    Gilbreth M., Yang P., Wang D., Frost J., Polverino A., Cobb M.H., Marcus S.
    Proc. Natl. Acad. Sci. U.S.A. 93:13802-13807(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBUNIT.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "The highly conserved protein methyltransferase, Skb1, is a mediator of hyperosmotic stress response in the fission yeast Schizosaccharomyces pombe."
    Bao S., Qyang Y., Yang P., Kim H., Du H., Bartholomeusz G., Henkel J., Pimental R.A., Verde F., Marcus S.
    J. Biol. Chem. 276:14549-14552(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSKB1_SCHPO
AccessioniPrimary (citable) accession number: P78963
Secondary accession number(s): O42946
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 11, 2001
Last modified: June 8, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.