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Protein

Protein arginine N-methyltransferase skb1

Gene

skb1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that can catalyze both the mono- and symmetric (type II) dimethylation of the guanidino nitrogens of arginine residues in target proteins (By similarity). Positively modulates the shk1 kinase function. May be a mediator of hyperosmotic stress response (PubMed:11278267).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei316S-adenosyl-L-methionineBy similarity1
Sitei319Critical for specifying symmetric addition of methyl groupsBy similarity1
Binding sitei379S-adenosyl-L-methionineBy similarity1
Active sitei422Proton donor/acceptorBy similarity1
Active sitei431Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to osmotic stress Source: PomBase
  • establishment or maintenance of bipolar cell polarity regulating cell shape Source: PomBase
  • lateral cortical node assembly Source: PomBase
  • peptidyl-arginine methylation, to symmetrical-dimethyl arginine Source: GO_Central
  • peptidyl-arginine N-methylation Source: PomBase
  • protein localization to lateral cortical node Source: PomBase
  • regulation of establishment or maintenance of bipolar cell polarity regulating cell shape Source: PomBase
  • regulation of transcription, DNA-templated Source: GO_Central

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein arginine N-methyltransferase skb11 Publication (EC:2.1.1.-By similarity)
Alternative name(s):
Shk1 kinase-binding protein 11 Publication
Type II protein arginine N-methyltransferaseBy similarity
Short name:
Type II PRMTBy similarity
Gene namesi
Name:skb11 Publication
Synonyms:rmt5Imported
ORF Names:SPBC16H5.11cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC16H5.11c
PomBaseiSPBC16H5.11c skb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002123461 – 645Protein arginine N-methyltransferase skb1Add BLAST645

Proteomic databases

MaxQBiP78963
PaxDbiP78963
PRIDEiP78963

Interactioni

Subunit structurei

Interacts with the N-terminal regulatory domain of shk1. Shk1, cdc42 and skb1 are able to form a ternary complex in vivo.1 Publication

Protein-protein interaction databases

BioGridi27663737 interactors.
IntActiP78963 1 interactor.
STRINGi4896.SPBC16H5.11c.1

Structurei

3D structure databases

ProteinModelPortaliP78963
SMRiP78963
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini300 – 608SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd BLAST309

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni325 – 326S-adenosyl-L-methionine bindingBy similarity2
Regioni406 – 407S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000175933
InParanoidiP78963
KOiK02516
OMAiFPMFFPT
OrthoDBiEOG092C0SQ8
PhylomeDBiP78963

Family and domain databases

InterProiView protein in InterPro
IPR025799 Arg_MeTrfase
IPR007857 Arg_MeTrfase_PRMT5
IPR035075 PRMT5
IPR035248 PRMT5_C
IPR035247 PRMT5_TIM
IPR029063 SAM-dependent_MTases
PANTHERiPTHR10738 PTHR10738, 1 hit
PfamiView protein in Pfam
PF05185 PRMT5, 1 hit
PF17286 PRMT5_C, 1 hit
PF17285 PRMT5_TIM, 1 hit
PIRSFiPIRSF015894 Skb1_MeTrfase, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51678 SAM_MT_PRMT, 1 hit

Sequencei

Sequence statusi: Complete.

P78963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRDGRIPQ YSIYPLPVPP MNSKTPTLGI VCSEGTISLS LEEGFEFVGV
60 70 80 90 100
PLSGEGLKLR VEALAPSERL QEFLDDEVAY HPEENVHKVV GLSSAWLELD
110 120 130 140 150
SEDTLIADRS EEVLLKEASY ASYCGLSSII LNGPTSPMNV MRYARAVSSA
160 170 180 190 200
LNSTMNLKFL VQLAIESGHE DYFETWKMWD TIRSACGYHP RLKVALELPP
210 220 230 240 250
ACSPPIELVN RWYAEPIEMI TMSCMAFVPN PNGYPVLGRK LRAIYALYLR
260 270 280 290 300
LNPRILLWDN DAPEKIGDSP DYSIYMKHLF DSQPPAPLVE DLADSYKDYL
310 320 330 340 350
QVPLQPLSYN LENITYEIFE RDPVKYAQYE QAIFSALMDR DESSVTRIAV
360 370 380 390 400
VGAGRGPLVD CALRAAISSS RTVDMIALEK NPNAFSMLLM RNRQDWAGKV
410 420 430 440 450
TLVFGDMRTW NPDYKIDILV SELLGSMGDN ELSPECLDGV QHVLDEETGI
460 470 480 490 500
CIPSSYISYV TPIMSPKLWS EARNMNDPNA FERQYVVLMN SFDFLAADDE
510 520 530 540 550
FRFQSLWSFH HPNKDSEVYT KNLHNKRFAS VRFQASSPGI LHGFAGYFEA
560 570 580 590 600
TLYKDISLSI MPATMEAKSP DMFSWFPIYM PIKKPMYVPE NSQLEFHMWR
610 620 630 640
LTDGMRVWFE WCANAYLVLR NGSQIKLSST EVHNISGKAF SCNMY
Length:645
Mass (Da):73,167
Last modified:January 11, 2001 - v2
Checksum:i288FEE7BDF61558D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45F → L in AAC49571 (PubMed:8943016).Curated1
Sequence conflicti134 – 138PTSPM → SNFTQC in AAC49571 (PubMed:8943016).Curated5
Sequence conflicti195A → G in AAC49571 (PubMed:8943016).Curated1
Sequence conflicti398G → A in AAC49571 (PubMed:8943016).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59684 Genomic DNA Translation: AAC49571.1
CU329671 Genomic DNA Translation: CAA17909.1
PIRiT39614
T48644
RefSeqiNP_595936.1, NM_001021844.2

Genome annotation databases

EnsemblFungiiSPBC16H5.11c.1; SPBC16H5.11c.1:pep; SPBC16H5.11c
GeneIDi2540099
KEGGispo:SPBC16H5.11c

Similar proteinsi

Entry informationi

Entry nameiSKB1_SCHPO
AccessioniPrimary (citable) accession number: P78963
Secondary accession number(s): O42946
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 11, 2001
Last modified: March 28, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome