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Protein

Glyceraldehyde-3-phosphate dehydrogenase 1

Gene

tdh1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2 (gpd3), Glyceraldehyde-3-phosphate dehydrogenase 1 (tdh1)
  2. Phosphoglycerate kinase (pgk1)
  3. Phosphoglycerate mutase (gpm1)
  4. Enolase 1-2 (eno102), Enolase 1-1 (eno101)
  5. Pyruvate kinase (pyk1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADBy similarity1
Binding sitei80NAD; via carbonyl oxygenBy similarity1
Active sitei152NucleophilePROSITE-ProRule annotation1
Sitei179Activates thiol group during catalysisBy similarity1
Binding sitei182Glyceraldehyde 3-phosphateBy similarity1
Binding sitei234Glyceraldehyde 3-phosphateBy similarity1
Binding sitei316NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 14NADBy similarity2

GO - Molecular functioni

GO - Biological processi

  • detection of oxidative stress Source: PomBase
  • glucose metabolic process Source: InterPro
  • glycolytic process Source: UniProtKB-UniPathway
  • positive regulation of phosphorelay signal transduction system involved in hydrogen peroxide mediated signaling pathway Source: PomBase
  • positive regulation of stress-activated MAPK cascade Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-SPO-70171. Glycolysis.
R-SPO-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 1 (EC:1.2.1.12)
Short name:
GAPDH 1
Gene namesi
Name:tdh1
Synonyms:gpd1
ORF Names:SPBC32F12.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC32F12.11.
PomBaseiSPBC32F12.11. tdh1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • fungal-type cell wall Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001455801 – 336Glyceraldehyde-3-phosphate dehydrogenase 1Add BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59Phosphoserine1 Publication1
Modified residuei125Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP78958.
PRIDEiP78958.

PTM databases

iPTMnetiP78958.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi277074. 22 interactors.
IntActiP78958. 5 interactors.
MINTiMINT-1213925.

Structurei

3D structure databases

ProteinModelPortaliP78958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni151 – 153Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni211 – 212Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000071678.
InParanoidiP78958.
KOiK00134.
OMAiAYKPEYQ.
OrthoDBiEOG092C2MZH.
PhylomeDBiP78958.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPKVGING FGRIGRIVLR NALVAKTIQV VAINDPFIDL EYMAYMFKYD
60 70 80 90 100
STHGRFDGSV EIKDGKLVID GNAIDVHNER DPADIKWSTS GADYVIESTG
110 120 130 140 150
VFTTQETASA HLKGGAKRVI ISAPSKDAPM YVVGVNEEKF NPSEKVISNA
160 170 180 190 200
SCTTNCLAPL AKVINDTFGI EEGLMTTVHA TTATQKTVDG PSKKDWRGGR
210 220 230 240 250
GASANIIPSS TGAAKAVGKV IPALNGKLTG MAFRVPTPDV SVVDLTVKLA
260 270 280 290 300
KPTNYEDIKA AIKAASEGPM KGVLGYTEDA VVSTDFCGDN HSSIFDASAG
310 320 330
IQLSPQFVKL VSWYDNEWGY SRRVVDLVAY TAAKDN
Length:336
Mass (Da):35,870
Last modified:May 1, 1997 - v1
Checksum:i38E58E194FF4747A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85332 mRNA. Translation: CAA59681.1.
CU329671 Genomic DNA. Translation: CAA19372.1.
PIRiT40235.
RefSeqiNP_596154.1. NM_001022073.2.

Genome annotation databases

EnsemblFungiiSPBC32F12.11.1; SPBC32F12.11.1:pep; SPBC32F12.11.
GeneIDi2540547.
KEGGispo:SPBC32F12.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85332 mRNA. Translation: CAA59681.1.
CU329671 Genomic DNA. Translation: CAA19372.1.
PIRiT40235.
RefSeqiNP_596154.1. NM_001022073.2.

3D structure databases

ProteinModelPortaliP78958.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277074. 22 interactors.
IntActiP78958. 5 interactors.
MINTiMINT-1213925.

PTM databases

iPTMnetiP78958.

Proteomic databases

MaxQBiP78958.
PRIDEiP78958.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC32F12.11.1; SPBC32F12.11.1:pep; SPBC32F12.11.
GeneIDi2540547.
KEGGispo:SPBC32F12.11.

Organism-specific databases

EuPathDBiFungiDB:SPBC32F12.11.
PomBaseiSPBC32F12.11. tdh1.

Phylogenomic databases

HOGENOMiHOG000071678.
InParanoidiP78958.
KOiK00134.
OMAiAYKPEYQ.
OrthoDBiEOG092C2MZH.
PhylomeDBiP78958.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
ReactomeiR-SPO-70171. Glycolysis.
R-SPO-70263. Gluconeogenesis.

Miscellaneous databases

PROiP78958.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P1_SCHPO
AccessioniPrimary (citable) accession number: P78958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.