Reviewed, UniProtKB/Swiss-Prot P78827 (ILV5_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable ketol-acid reductoisomerase, mitochondrial
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid reductoisomerase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilv5
ORF Names:	SPBC56F2.12

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Subcellular location	MitochondrionBy similarity.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 404		Probable ketol-acid reductoisomerase, mitochondrial		PRO_0000015633
Sites
	Active site	178	1	Potential
Amino acid modifications
	Modified residue	261	1	Phosphoserine
Experimental info
	Sequence conflict	38	1	S → R in BAA13837. Ref.1
	Sequence conflict	92	1	Y → S in BAA13837. Ref.1
	Sequence conflict	275	1	N → P in BAA13837. Ref.1
	Sequence conflict	297	1	N → P in BAA13837. Ref.1
	Sequence conflict	394	1	V → G in BAA13837. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P78827-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 9AB3674C71AD6FEB	FASTA	404	45,189
	10 20 30 40 50 60 MSFRNSSRMA MKALRTMGSR RLATRSMSVM ARTIAAPSMR FAPRMTAPLM QTRGMRVMDF 70 80 90 100 110 120 AGTKENVWER SDWPREKLVD YFKNDTLAII GYGSQGHGQG LNARDQGLNV IVGVRKDGAS 130 140 150 160 170 180 WKQAIEDGWV PGKTLFPVEE AIKKGSIIMN LLSDAAQTET WPKIAPLITK GKTLYFSHGF 190 200 210 220 230 240 SVIFKDQTKI HPPKDVDVIL VAPKGSGRTV RTLFKEGRGI NSSFAVYQDV TGKAQEKAIG 250 260 270 280 290 300 LAVAVGSGFI YQTTFKKEVI SDLVGERGCL MGGINGLFLA QYQVLRERGH SPAEAFNETV 310 320 330 340 350 360 EEATQSLYPL IGKYGLDYMF AACSTTARRG AIDWTPRFLE ANKKVLNELY DNVENGNEAK 370 380 390 400 RSLEYNSAPN YRELYDKELE EIRNLEIWKA GEVVRSLRPE HNKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: PR745.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"S.pombe ILV5 homolog." Kawamukai M. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 262-404.
[4]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-261, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D89175 mRNA. Translation: BAA13837.1. CU329671 Genomic DNA. Translation: CAA18891.1. AB009603 mRNA. Translation: BAA24000.1.
PIR	T40532.
RefSeq	NP_001018845.2.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Genome annotation databases
GeneID	3361354.
KEGG	spo:SPBC56F2.12.
Organism-specific databases
GeneDB_Spombe	SPBC56F2.12.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004692-MON.
Gene expression databases
ArrayExpress	P78827.
Family and domain databases
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DHase_multihelical. IPR013116. IlvN. IPR016207. KetolA_reductoisomerase_fun. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000119. Ilv5_fungal. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILV5_SCHPO

Accession

Primary (citable) accession number: P78827
Secondary accession number(s): O42619

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 11, 2001
Last modified:	November 25, 2008
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)