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P78812

- 6PGD_SCHPO

UniProt

P78812 - 6PGD_SCHPO

Protein

6-phosphogluconate dehydrogenase, decarboxylating

Gene

SPBC660.16

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (11 Jan 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH.By similarity

    Catalytic activityi

    6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei106 – 1061NADPBy similarity
    Binding sitei106 – 1061SubstrateBy similarity
    Active sitei187 – 1871Proton acceptorBy similarity
    Active sitei194 – 1941Proton donorBy similarity
    Binding sitei195 – 1951SubstrateBy similarity
    Binding sitei264 – 2641Substrate; via amide nitrogenBy similarity
    Binding sitei291 – 2911SubstrateBy similarity
    Binding sitei449 – 4491Substrate; shared with dimeric partnerBy similarity
    Binding sitei455 – 4551Substrate; shared with dimeric partnerBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 186NADPBy similarity
    Nucleotide bindingi36 – 383NADPBy similarity
    Nucleotide bindingi78 – 803NADPBy similarity

    GO - Molecular functioni

    1. NADP binding Source: PomBase
    2. phosphogluconate dehydrogenase (decarboxylating) activity Source: PomBase

    GO - Biological processi

    1. D-gluconate metabolic process Source: UniProtKB-KW
    2. pentose-phosphate shunt, oxidative branch Source: PomBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Gluconate utilization, Pentose shunt

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    ReactomeiREACT_212835. Pentose phosphate pathway (hexose monophosphate shunt).
    UniPathwayiUPA00115; UER00410.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phosphogluconate dehydrogenase, decarboxylating (EC:1.1.1.44)
    Gene namesi
    ORF Names:SPBC660.16
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC660.16.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. mitochondrion Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4924926-phosphogluconate dehydrogenase, decarboxylatingPRO_0000090074Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei107 – 1071Phosphoserine1 Publication
    Modified residuei215 – 2151Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP78812.
    PaxDbiP78812.
    PRIDEiP78812.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi277661. 6 interactions.
    MINTiMINT-4691318.
    STRINGi4896.SPBC660.16-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP78812.
    SMRiP78812. Positions 7-479.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni132 – 1343Substrate bindingBy similarity
    Regioni190 – 1912Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0362.
    HOGENOMiHOG000255147.
    KOiK00033.
    OMAiRVASENY.
    OrthoDBiEOG7F252Z.
    PhylomeDBiP78812.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    1.20.5.320. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR006114. 6PGDH_C.
    IPR006113. 6PGDH_decarbox.
    IPR006115. 6PGDH_NADP-bd.
    IPR006184. 6PGdom_BS.
    IPR013328. DH_multihelical.
    IPR012284. Fibritin/6PGD_C-extension.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00393. 6PGD. 1 hit.
    PF03446. NAD_binding_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000109. 6PGD. 1 hit.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    TIGRFAMsiTIGR00873. gnd. 1 hit.
    PROSITEiPS00461. 6PGD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P78812-1 [UniParc]FASTAAdd to Basket

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    MSQKEVADFG LIGLAVMGQN LILNGADKGF TVCCYNRTTS RVDEFLANEA    50
    KGKSIVGAHS LEEFVSKLKK PRVCILLVKA GKPVDYLIEG LAPLLEKGDI 100
    IVDGGNSHYP DTTRRCEELA KKGILFVGSG VSGGEEGARY GPSLMPGGNP 150
    AAWPRIKPIF QTLAAKAGNN EPCCDWVGEQ GAGHYVKMVH NGIEYGDMQL 200
    ICETYDIMKR GLGMSCDEIA DVFEKWNTGK LDSFLIEITR DVLRYKADDG 250
    KPLVEKILDA AGQKGTGKWT AQNALEMGTP VSLITEAVFA RCLSSLKSER 300
    VRASKKLTGP NTKFTGDKKQ LIDDLEDALY ASKIISYAQG FMLMREAAKE 350
    YGWKLNNAGI ALMWRGGCII RSVFLKDITE AFREDPNLES ILFHPFFTNG 400
    VEKAQAGWRR VVAQAAMLGI PVPATSTGLS FYDGYRSAVL PANLLQAQRD 450
    YFGAHTFRVL PEAADKSLPA DKDIHINWTG HGGNISATTY DA 492
    Length:492
    Mass (Da):53,680
    Last modified:January 11, 2001 - v2
    Checksum:iF55F342957A9D3E1
    GO

    Sequence cautioni

    The sequence BAA13823.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti219 – 2202IA → ST in BAA13823. (PubMed:9501991)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89161 mRNA. Translation: BAA13823.1. Different initiation.
    CU329671 Genomic DNA. Translation: CAA22536.1.
    PIRiT40628.
    T42523.
    RefSeqiNP_595095.1. NM_001021002.2.

    Genome annotation databases

    EnsemblFungiiSPBC660.16.1; SPBC660.16.1:pep; SPBC660.16.
    GeneIDi2541146.
    KEGGispo:SPBC660.16.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89161 mRNA. Translation: BAA13823.1 . Different initiation.
    CU329671 Genomic DNA. Translation: CAA22536.1 .
    PIRi T40628.
    T42523.
    RefSeqi NP_595095.1. NM_001021002.2.

    3D structure databases

    ProteinModelPortali P78812.
    SMRi P78812. Positions 7-479.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277661. 6 interactions.
    MINTi MINT-4691318.
    STRINGi 4896.SPBC660.16-1.

    Proteomic databases

    MaxQBi P78812.
    PaxDbi P78812.
    PRIDEi P78812.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC660.16.1 ; SPBC660.16.1:pep ; SPBC660.16 .
    GeneIDi 2541146.
    KEGGi spo:SPBC660.16.

    Organism-specific databases

    PomBasei SPBC660.16.

    Phylogenomic databases

    eggNOGi COG0362.
    HOGENOMi HOG000255147.
    KOi K00033.
    OMAi RVASENY.
    OrthoDBi EOG7F252Z.
    PhylomeDBi P78812.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00410 .
    Reactomei REACT_212835. Pentose phosphate pathway (hexose monophosphate shunt).

    Miscellaneous databases

    NextBioi 20802259.
    PROi P78812.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    1.20.5.320. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR006114. 6PGDH_C.
    IPR006113. 6PGDH_decarbox.
    IPR006115. 6PGDH_NADP-bd.
    IPR006184. 6PGdom_BS.
    IPR013328. DH_multihelical.
    IPR012284. Fibritin/6PGD_C-extension.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00393. 6PGD. 1 hit.
    PF03446. NAD_binding_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000109. 6PGD. 1 hit.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    TIGRFAMsi TIGR00873. gnd. 1 hit.
    PROSITEi PS00461. 6PGD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
      Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
      DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: PR745.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107 AND SER-215, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry namei6PGD_SCHPO
    AccessioniPrimary (citable) accession number: P78812
    Secondary accession number(s): Q9UQW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3