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Protein

6-phosphogluconate dehydrogenase, decarboxylating

Gene

SPBC660.16

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH.By similarity

Catalytic activityi

6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei106 – 1061NADPBy similarity
Binding sitei106 – 1061SubstrateBy similarity
Active sitei187 – 1871Proton acceptorBy similarity
Active sitei194 – 1941Proton donorBy similarity
Binding sitei195 – 1951SubstrateBy similarity
Binding sitei264 – 2641Substrate; via amide nitrogenBy similarity
Binding sitei291 – 2911SubstrateBy similarity
Binding sitei449 – 4491Substrate; shared with dimeric partnerBy similarity
Binding sitei455 – 4551Substrate; shared with dimeric partnerBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 186NADPBy similarity
Nucleotide bindingi36 – 383NADPBy similarity
Nucleotide bindingi78 – 803NADPBy similarity

GO - Molecular functioni

  1. NADP binding Source: PomBase
  2. phosphogluconate dehydrogenase (decarboxylating) activity Source: PomBase

GO - Biological processi

  1. D-gluconate metabolic process Source: UniProtKB-KW
  2. pentose-phosphate shunt, oxidative branch Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Gluconate utilization, Pentose shunt

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_351168. Pentose phosphate pathway (hexose monophosphate shunt).
UniPathwayiUPA00115; UER00410.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphogluconate dehydrogenase, decarboxylating (EC:1.1.1.44)
Gene namesi
ORF Names:SPBC660.16
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC660.16.
PomBaseiSPBC660.16.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. mitochondrion Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4924926-phosphogluconate dehydrogenase, decarboxylatingPRO_0000090074Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei107 – 1071Phosphoserine1 Publication
Modified residuei215 – 2151Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP78812.
PaxDbiP78812.
PRIDEiP78812.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi277661. 5 interactions.
MINTiMINT-4691318.
STRINGi4896.SPBC660.16-1.

Structurei

3D structure databases

ProteinModelPortaliP78812.
SMRiP78812. Positions 7-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1343Substrate bindingBy similarity
Regioni190 – 1912Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0362.
HOGENOMiHOG000255147.
InParanoidiP78812.
KOiK00033.
OMAiSDEYNWD.
OrthoDBiEOG7F252Z.
PhylomeDBiP78812.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR012284. 6PGD_dom_3.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_Gnd/GntZ.
IPR006115. 6PGDH_NADP-bd.
IPR006184. 6PGdom_BS.
IPR016040. NAD(P)-bd_dom.
IPR006183. Pgluconate_DH.
[Graphical view]
PfamiPF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000109. 6PGD. 1 hit.
PRINTSiPR00076. 6PGDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00873. gnd. 1 hit.
PROSITEiPS00461. 6PGD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKEVADFG LIGLAVMGQN LILNGADKGF TVCCYNRTTS RVDEFLANEA
60 70 80 90 100
KGKSIVGAHS LEEFVSKLKK PRVCILLVKA GKPVDYLIEG LAPLLEKGDI
110 120 130 140 150
IVDGGNSHYP DTTRRCEELA KKGILFVGSG VSGGEEGARY GPSLMPGGNP
160 170 180 190 200
AAWPRIKPIF QTLAAKAGNN EPCCDWVGEQ GAGHYVKMVH NGIEYGDMQL
210 220 230 240 250
ICETYDIMKR GLGMSCDEIA DVFEKWNTGK LDSFLIEITR DVLRYKADDG
260 270 280 290 300
KPLVEKILDA AGQKGTGKWT AQNALEMGTP VSLITEAVFA RCLSSLKSER
310 320 330 340 350
VRASKKLTGP NTKFTGDKKQ LIDDLEDALY ASKIISYAQG FMLMREAAKE
360 370 380 390 400
YGWKLNNAGI ALMWRGGCII RSVFLKDITE AFREDPNLES ILFHPFFTNG
410 420 430 440 450
VEKAQAGWRR VVAQAAMLGI PVPATSTGLS FYDGYRSAVL PANLLQAQRD
460 470 480 490
YFGAHTFRVL PEAADKSLPA DKDIHINWTG HGGNISATTY DA
Length:492
Mass (Da):53,680
Last modified:January 11, 2001 - v2
Checksum:iF55F342957A9D3E1
GO

Sequence cautioni

The sequence BAA13823.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti219 – 2202IA → ST in BAA13823 (PubMed:9501991).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89161 mRNA. Translation: BAA13823.1. Different initiation.
CU329671 Genomic DNA. Translation: CAA22536.1.
PIRiT40628.
T42523.
RefSeqiNP_595095.1. NM_001021002.2.

Genome annotation databases

EnsemblFungiiSPBC660.16.1; SPBC660.16.1:pep; SPBC660.16.
GeneIDi2541146.
KEGGispo:SPBC660.16.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89161 mRNA. Translation: BAA13823.1. Different initiation.
CU329671 Genomic DNA. Translation: CAA22536.1.
PIRiT40628.
T42523.
RefSeqiNP_595095.1. NM_001021002.2.

3D structure databases

ProteinModelPortaliP78812.
SMRiP78812. Positions 7-479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277661. 5 interactions.
MINTiMINT-4691318.
STRINGi4896.SPBC660.16-1.

Proteomic databases

MaxQBiP78812.
PaxDbiP78812.
PRIDEiP78812.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC660.16.1; SPBC660.16.1:pep; SPBC660.16.
GeneIDi2541146.
KEGGispo:SPBC660.16.

Organism-specific databases

EuPathDBiFungiDB:SPBC660.16.
PomBaseiSPBC660.16.

Phylogenomic databases

eggNOGiCOG0362.
HOGENOMiHOG000255147.
InParanoidiP78812.
KOiK00033.
OMAiSDEYNWD.
OrthoDBiEOG7F252Z.
PhylomeDBiP78812.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00410.
ReactomeiREACT_351168. Pentose phosphate pathway (hexose monophosphate shunt).

Miscellaneous databases

NextBioi20802259.
PROiP78812.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR012284. 6PGD_dom_3.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_Gnd/GntZ.
IPR006115. 6PGDH_NADP-bd.
IPR006184. 6PGdom_BS.
IPR016040. NAD(P)-bd_dom.
IPR006183. Pgluconate_DH.
[Graphical view]
PfamiPF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000109. 6PGD. 1 hit.
PRINTSiPR00076. 6PGDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00873. gnd. 1 hit.
PROSITEiPS00461. 6PGD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: PR745.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107 AND SER-215, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry namei6PGD_SCHPO
AccessioniPrimary (citable) accession number: P78812
Secondary accession number(s): Q9UQW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 11, 2001
Last modified: April 29, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.