P78811 (UGPA1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable UTP--glucose-1-phosphate uridylyltransferase
EC=2.7.7.9

Alternative name(s):
UDP-glucose pyrophosphorylase
Short name=UDPGP
Short name=UGPase

Gene names

Name:	fuy1
ORF Names:	SPCC1322.04

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a central role as a glucosyl donor in cellular metabolic pathways. Ref.5
Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose. Ref.5
Subunit structure	Homooctamer By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.3.
Sequence similarities	Belongs to the UDPGP type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UDP-glucose metabolic process Inferred from mutant phenotype Ref.5. Source: PomBase cellular response to stress Inferred from expression pattern PubMed 12529438. Source: PomBase
Cellular_component	cytosol Inferred from direct assay Ref.3. Source: PomBase nucleus Inferred from direct assay Ref.3. Source: PomBase
Molecular_function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from mutant phenotype Ref.5. Source: PomBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Probable UTP--glucose-1-phosphate uridylyltransferase		PRO_0000185763
Regions
	Region	455 – 506	52	Oligomerization By similarity
Sites
	Active site	394	1	By similarity
	Metal binding	129	1	Magnesium By similarity
	Metal binding	252	1	Magnesium By similarity
Amino acid modifications
	Modified residue	15	1	Phosphoserine Ref.4
	Modified residue	17	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P78811 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: CC4B7EF856D25630	FASTA	506	56,430
	10 20 30 40 50 60 MDLAPRPLGR QHSKSQSAFA FDNTATSIAA STMKNELNHL ASTVKDPLAK KAFQKEMDNF 70 80 90 100 110 120 FSLFSRYLQE DARGSEINWD LVESPKPEQV VEYDTITEAG GLSRDYLNKL AVLKLNGGLG 130 140 150 160 170 180 TTMGCVGPKS IIEVRDGNSF LDLSVRQIEH LNRKYNVNVP FVLMNSFNTD EATAKVIKKY 190 200 210 220 230 240 EAHKIDILTF NQSRYPRVHK ETLLPVPHTA DSAIDEWYPP GHGDVFEALT NSGIIDTLIA 250 260 270 280 290 300 QGKEYLFVSN IDNLGAVVDL NILNHMVETN AEYLMELTNK TKADVKGGTL IDYDGNVRLL 310 320 330 340 350 360 EIAQVPPQHV EEFKSIKKFK YFNTNNLWFH LPSVKRVVNN HELSMEIIPN KKTIKHKGEN 370 380 390 400 410 420 INIIQLETAA GAAIRHFKNA HGVNVPRRRF LPVKTCSDLL LVKSDLYSIN HGQVEMNPRR 430 440 450 460 470 480 FGGTAPLVKL GAHFKKVADF SAHIPSIPKI LELDHLTITG DVNIGRNVTL KGTVIIVASD 490 500 ANRIDIPNGS VLENCVITGN LNILEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 203-506. Strain: PR745.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15 AND SER-17, MASS SPECTROMETRY.
[5]	"Production of ibuprofen acyl glucosides by human UGT2B7." Buchheit D., Dragan C.A., Schmitt E.I., Bureik M. Drug Metab. Dispos. 39:2174-2181(2011) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAA22857.1. D89160 mRNA. Translation: BAA13822.1.
PIR	T40935. T42521.
RefSeq	NP_588132.1. NM_001023122.2.
3D structure databases
ProteinModelPortal	P78811.
SMR	P78811. Positions 28-506.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4691308.
STRING	4896.SPCC1322.04-1.
Proteomic databases
PaxDb	P78811.
PRIDE	P78811.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC1322.04.1; SPCC1322.04.1:pep; SPCC1322.04.
GeneID	2538877.
KEGG	spo:SPCC1322.04.
Organism-specific databases
PomBase	SPCC1322.04.
Phylogenomic databases
eggNOG	COG4284.
HOGENOM	HOG000113618.
KO	K00963.
OMA	PPNGKRC.
OrthoDB	EOG4KSST8.
Family and domain databases
InterPro	IPR002618. UDPGP_trans. IPR016267. UDPGP_trans_subgr. [Graphical view]
PANTHER	PTHR11952. PTHR11952. 1 hit. PTHR11952:SF1. PTHR11952:SF1. 1 hit.
Pfam	PF01704. UDPGP. 1 hit. [Graphical view]
PIRSF	PIRSF000806. UDPGP. 1 hit.
ProtoNet	Search...
Other
NextBio	20800057.

Entry information

Entry name

UGPA1_SCHPO

Accession

Primary (citable) accession number: P78811

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 11, 2001
Last modified:	May 29, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program