Reviewed, UniProtKB/Swiss-Prot P78804 (DHE4_SCHPO)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase

Gene names

Name:	gdh1
ORF Names:	SPCC622.12c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	NADP-specific glutamate dehydrogenase		PRO_0000182794
Sites
	Active site	113	1	By similarity
Amino acid modifications
	Modified residue	252	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	P78804-1 [UniParc]. Last modified August 1, 1999. Version 2. Checksum: DC05673E6A3433F5	FASTA	451	48,790
	10 20 30 40 50 60 MSTPYEPEFQ QAYKEIVGSI ESSKLFEVHP ELKRVLPIIS IPERVLEFRV TWEDDKGNCR 70 80 90 100 110 120 VNTGYRVQFN SALGPYKGGL RFHPSVNLSI LKFLGFEQIF KNALTGLPMG GGKGGSDFDP 130 140 150 160 170 180 KGKSDNEIRR FSQAFMRQLF RYIGPQTDVP AGDIGVTGFV VMHMFGEYKR LRNEYSGVVT 190 200 210 220 230 240 GKHMLTGGSN IRPEATGYGV VYYVKHMIEH RTKGAETLKG KRVAISGSGN VAQYAALKCI 250 260 270 280 290 300 QEGAIVKSIS DSKGVLIAKT AEGLVPEEIH EIMALKEKRA SIADSASLCK KHHYIAGARP 310 320 330 340 350 360 WTNVGEIDIA LPCATQNEVS GEEAAALIKQ GCRYVAEGSN MGSSAEAVEV FEKSRASGEG 370 380 390 400 410 420 CWLAPGKAAN AGGVAVSGLE MAQNAQFSTW THAEVDAKLA GIMQNIFEQS TDVASKYCDS 430 440 450 GSNNIPSLVD GANIAGFLKV ATAMQAVGDW W

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-447. Strain: PR745.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAA21868.1. D89153 mRNA. Translation: BAA13815.1.
PIR	T41492.
RefSeq	NP_588184.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Genome annotation databases
GeneID	2539147.
KEGG	spo:SPCC622.12c.
NMPDR	fig|4896.1.peg.522.
Organism-specific databases
GeneDB_Spombe	SPCC622.12c.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000123-MON.
Gene expression databases
ArrayExpress	P78804.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR014362. Glu_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_SCHPO

Accession

Primary (citable) accession number: P78804

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	August 1, 1999
Last modified:	November 25, 2008
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)