P78773 (ACSA_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

ORF Names:

SPCC191.02c, SPCC417.14c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	662 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	acetate fermentation Inferred from sequence or structural similarity. Source: GeneDB_Spombe acetyl-CoA metabolic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe mitochondrion Inferred from sequence or structural similarity. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 662	662	Probable acetyl-coenzyme A synthetase		PRO_0000208419
Amino acid modifications
	Modified residue	596	1	Phosphothreonine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P78773 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: 02FD119ABB622583	FASTA	662	73,036
	10 20 30 40 50 60 MTKNPVDHTL IIEPPVRLHG DPTVPKPNIA SLDEYKRMYE ESINDPSTFW GNMARDMMTW 70 80 90 100 110 120 DKQFSTVVQG SIDKADSAWF ADGAISPCYN LVDRHAIARP DAVALIYEAD EPNQGRYITY 130 140 150 160 170 180 RELLASVSQC AGALQSMGVG MGDRVAIYMP MIPETIIAML AIVRLGAIHS VIFAGFSAES 190 200 210 220 230 240 VADRVNDSEC KVIITADESH RGGKRIPLKG VVNKALTECP TIKKVLVFQR SAEPTASMVE 250 260 270 280 290 300 GRDVWWHDII PKFPRYCPPA VVNPEHPLFL LYTSGSTGKP KGVVHCTGGY LLGAAATCKY 310 320 330 340 350 360 VFDLHPTDRM GCAGDVGWIT GHTYIVYGPL MLGAATLVFE STPAYPDYSR YWSVVERHRL 370 380 390 400 410 420 TQWYIAPTAI RLLQRAGNEF VKHDRSSLRV LGSVGEPIAP ESFMWYYEVV GEKRCAVADT 430 440 450 460 470 480 YWQTETGSHI VTSLGPVTPM KPGSATLPFF GIDAVIIDPL TGKIIEGNDV EGVLAIRSPW 490 500 510 520 530 540 PSAARTVWRG HDRYIDTYLK PYPGFYFTGD GATRDKDGYI WIRGRVDDVV NISGHRLSTA 550 560 570 580 590 600 EIEAALLSHD AVAESAVVGV HDELTGQAVN AFILLKPGYE ATVELEKELI MAVRSTIGPF 610 620 630 640 650 660 ASPRKLIFSD LPKTRSGKIM RRILRKILAG EVDQIGDLST LADPKVVEHI IHAVHYAHQK KP

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-662. Strain: PR745.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-596, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAA22660.1. D89121 mRNA. Translation: BAA13783.1.
PIR	T41215.
RefSeq	NP_588291.2. NM_001023281.1.
3D structure databases
ProteinModelPortal	P78773.
SMR	P78773. Positions 33-654.
ModBase	Search...
Protein-protein interaction databases
STRING	P78773.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC191.02c.1; SPCC191.02c.1:pep; SPCC191.02c.
GeneID	2539546.
KEGG	spo:SPCC191.02c.
Organism-specific databases
GeneDB_Spombe	SPCC191.02c.
Phylogenomic databases
eggNOG	fuNOG05220.
GeneTree	EFGT00050000001162.
HOGENOM	HBG547964.
OMA	KENDTYW.
OrthoDB	EOG4M0J8W.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000485-MONOMER.
Gene expression databases
ArrayExpress	P78773.
Family and domain databases
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_SCHPO

Accession

Primary (citable) accession number: P78773

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program