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P78753

- ASNS_SCHPO

UniProt

P78753 - ASNS_SCHPO

Protein

Probable asparagine synthetase [glutamine-hydrolyzing]

Gene

asn1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei99 – 991GlutamineBy similarity
    Binding sitei235 – 2351ATP; via carbonyl oxygenBy similarity
    Binding sitei280 – 2801ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei356 – 3561Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi354 – 3552ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: PomBase
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. asparagine biosynthetic process Source: PomBase
    2. glutamine metabolic process Source: UniProtKB-KW
    3. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_188302. ATF4 activates genes.
    REACT_221562. Amino acid synthesis and interconversion (transamination).
    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase
    Gene namesi
    Name:asn1
    ORF Names:SPBC119.10
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC119.10.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Causes asparagine auxotrophy.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 557556Probable asparagine synthetase [glutamine-hydrolyzing]PRO_0000056916Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei391 – 3911Phosphoserine1 Publication
    Modified residuei489 – 4891Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP78753.
    PaxDbiP78753.

    Interactioni

    Protein-protein interaction databases

    BioGridi276257. 2 interactions.
    MINTiMINT-4691132.
    STRINGi4896.SPBC119.10-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP78753.
    SMRiP78753. Positions 1-529.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 188187Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini196 – 531336Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 545Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0367.
    HOGENOMiHOG000027493.
    KOiK01953.
    OMAiYWYRLKF.
    OrthoDBiEOG73JM46.
    PhylomeDBiP78753.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P78753-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGILAVHHV AEDIEAFKPK ALHLSKQLRH RGPDWSGKAI RNQTILCHER    50
    LAIVGVESGA QPLVSDDGKL VLTVNGEIYN HLKLRENLKG NYKFKTYSDC 100
    EVILYLYREH GPACANMLDG MFSWVLYDQD KDKVVAARDP IGITTLYQGF 150
    SSDSPDTAYF ASELKALHPV CDKIIAFPPG HYYDSETKQT VRYFKPSWWD 200
    ENKIPSNPVD YKLLRETLEA SVRKRLMAEV PYGVLLSGGL DSSLIASIAA 250
    RETEKLANST SQSEEARTIT AWPKLHSFAI GLPGSPDLLA ARKVADFLHT 300
    FHHEHTFTID EGLDALRDVI YHLETYDVTT IRASTPMYLL SRKIKAQGVK 350
    MVLSGEGSDE IFGGYLYFGN APSREAFHSE CVRRVKNLHL SDCLRANKST 400
    MAWGLEARVP FLDKDFLEVA LNIDPEEKMY INGRKEKYIL RKAFDTTHDS 450
    SLQPYLPQDI LWRQKEQFSD GVGYSWIDAL KDTAELCISD DEFALPRREW 500
    GDDIPTTKEA FWYRKLFDEI FPRQCADTVM RWVPKAEWGC PEDPSGRYQA 550
    GHVAALK 557
    Length:557
    Mass (Da):63,241
    Last modified:January 23, 2007 - v3
    Checksum:iE561EF981A92FBAC
    GO

    Sequence cautioni

    The sequence BAA13764.1 differs from that shown. Reason: Frameshift at position 313.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89101 mRNA. Translation: BAA13764.1. Frameshift.
    CU329671 Genomic DNA. Translation: CAA17925.1.
    PIRiT39308.
    RefSeqiNP_595291.1. NM_001021198.2.

    Genome annotation databases

    EnsemblFungiiSPBC119.10.1; SPBC119.10.1:pep; SPBC119.10.
    GeneIDi2539704.
    KEGGispo:SPBC119.10.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89101 mRNA. Translation: BAA13764.1 . Frameshift.
    CU329671 Genomic DNA. Translation: CAA17925.1 .
    PIRi T39308.
    RefSeqi NP_595291.1. NM_001021198.2.

    3D structure databases

    ProteinModelPortali P78753.
    SMRi P78753. Positions 1-529.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276257. 2 interactions.
    MINTi MINT-4691132.
    STRINGi 4896.SPBC119.10-1.

    Protein family/group databases

    MEROPSi C44.976.

    Proteomic databases

    MaxQBi P78753.
    PaxDbi P78753.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC119.10.1 ; SPBC119.10.1:pep ; SPBC119.10 .
    GeneIDi 2539704.
    KEGGi spo:SPBC119.10.

    Organism-specific databases

    PomBasei SPBC119.10.

    Phylogenomic databases

    eggNOGi COG0367.
    HOGENOMi HOG000027493.
    KOi K01953.
    OMAi YWYRLKF.
    OrthoDBi EOG73JM46.
    PhylomeDBi P78753.

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .
    Reactomei REACT_188302. ATF4 activates genes.
    REACT_221562. Amino acid synthesis and interconversion (transamination).

    Miscellaneous databases

    NextBioi 20800856.
    PROi P78753.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
      Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
      DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: PR745.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. "Six new amino acid-auxotrophic markers for targeted gene integration and disruption in fission yeast."
      Ma Y., Sugiura R., Saito M., Koike A., Sio S.O., Fujita Y., Takegawa K., Kuno T.
      Curr. Genet. 52:97-105(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391 AND SER-489, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiASNS_SCHPO
    AccessioniPrimary (citable) accession number: P78753
    Secondary accession number(s): O42902
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3