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Protein

Probable asparagine synthetase [glutamine-hydrolyzing]

Gene

asn1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Probable asparagine synthetase [glutamine-hydrolyzing] (asn1)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei99GlutamineBy similarity1
Binding sitei235ATP; via carbonyl oxygenBy similarity1
Binding sitei280ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei356Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi354 – 355ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • asparagine biosynthetic process Source: PomBase
  • glutamine metabolic process Source: GO_Central
  • L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:asn1
ORF Names:SPBC119.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC119.10.
PomBaseiSPBC119.10. asn1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Causes asparagine auxotrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569162 – 557Probable asparagine synthetase [glutamine-hydrolyzing]Add BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei391Phosphoserine1 Publication1
Modified residuei489Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP78753.
PRIDEiP78753.

PTM databases

iPTMnetiP78753.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi276257. 1 interactor.
MINTiMINT-4691132.

Structurei

3D structure databases

ProteinModelPortaliP78753.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 188Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST187
Domaini196 – 531Asparagine synthetaseAdd BLAST336

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 54Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000027493.
InParanoidiP78753.
KOiK01953.
OMAiYAKIHEQ.
OrthoDBiEOG092C11PG.
PhylomeDBiP78753.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P78753-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILAVHHV AEDIEAFKPK ALHLSKQLRH RGPDWSGKAI RNQTILCHER
60 70 80 90 100
LAIVGVESGA QPLVSDDGKL VLTVNGEIYN HLKLRENLKG NYKFKTYSDC
110 120 130 140 150
EVILYLYREH GPACANMLDG MFSWVLYDQD KDKVVAARDP IGITTLYQGF
160 170 180 190 200
SSDSPDTAYF ASELKALHPV CDKIIAFPPG HYYDSETKQT VRYFKPSWWD
210 220 230 240 250
ENKIPSNPVD YKLLRETLEA SVRKRLMAEV PYGVLLSGGL DSSLIASIAA
260 270 280 290 300
RETEKLANST SQSEEARTIT AWPKLHSFAI GLPGSPDLLA ARKVADFLHT
310 320 330 340 350
FHHEHTFTID EGLDALRDVI YHLETYDVTT IRASTPMYLL SRKIKAQGVK
360 370 380 390 400
MVLSGEGSDE IFGGYLYFGN APSREAFHSE CVRRVKNLHL SDCLRANKST
410 420 430 440 450
MAWGLEARVP FLDKDFLEVA LNIDPEEKMY INGRKEKYIL RKAFDTTHDS
460 470 480 490 500
SLQPYLPQDI LWRQKEQFSD GVGYSWIDAL KDTAELCISD DEFALPRREW
510 520 530 540 550
GDDIPTTKEA FWYRKLFDEI FPRQCADTVM RWVPKAEWGC PEDPSGRYQA

GHVAALK
Length:557
Mass (Da):63,241
Last modified:January 23, 2007 - v3
Checksum:iE561EF981A92FBAC
GO

Sequence cautioni

The sequence BAA13764 differs from that shown. Reason: Frameshift at position 313.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89101 mRNA. Translation: BAA13764.1. Frameshift.
CU329671 Genomic DNA. Translation: CAA17925.1.
PIRiT39308.
RefSeqiNP_595291.1. NM_001021198.2.

Genome annotation databases

EnsemblFungiiSPBC119.10.1; SPBC119.10.1:pep; SPBC119.10.
GeneIDi2539704.
KEGGispo:SPBC119.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89101 mRNA. Translation: BAA13764.1. Frameshift.
CU329671 Genomic DNA. Translation: CAA17925.1.
PIRiT39308.
RefSeqiNP_595291.1. NM_001021198.2.

3D structure databases

ProteinModelPortaliP78753.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276257. 1 interactor.
MINTiMINT-4691132.

Protein family/group databases

MEROPSiC44.976.

PTM databases

iPTMnetiP78753.

Proteomic databases

MaxQBiP78753.
PRIDEiP78753.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC119.10.1; SPBC119.10.1:pep; SPBC119.10.
GeneIDi2539704.
KEGGispo:SPBC119.10.

Organism-specific databases

EuPathDBiFungiDB:SPBC119.10.
PomBaseiSPBC119.10. asn1.

Phylogenomic databases

HOGENOMiHOG000027493.
InParanoidiP78753.
KOiK01953.
OMAiYAKIHEQ.
OrthoDBiEOG092C11PG.
PhylomeDBiP78753.

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.
ReactomeiR-SPO-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

PROiP78753.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASNS_SCHPO
AccessioniPrimary (citable) accession number: P78753
Secondary accession number(s): O42902
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.