Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P78753 (ASNS_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable asparagine synthetase [glutamine-hydrolyzing]

EC=6.3.5.4
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene names
Name:asn1
ORF Names:SPBC119.10
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length557 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Subcellular location

Cytoplasm. Nucleus Ref.3.

Disruption phenotype

Causes asparagine auxotrophy. Ref.4

Sequence similarities

Contains 1 asparagine synthetase domain.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence caution

The sequence BAA13764.1 differs from that shown. Reason: Frameshift at position 313.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 557556Probable asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056916

Regions

Domain2 – 188187Glutamine amidotransferase type-2
Domain196 – 531336Asparagine synthetase
Nucleotide binding354 – 3552ATP By similarity
Region50 – 545Glutamine binding By similarity
Region75 – 773Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site991Glutamine By similarity
Binding site2351ATP; via carbonyl oxygen By similarity
Binding site2801ATP; via amide nitrogen and carbonyl oxygen By similarity
Site3561Important for beta-aspartyl-AMP intermediate formation By similarity

Amino acid modifications

Modified residue3911Phosphoserine Ref.5
Modified residue4891Phosphoserine Ref.5

Sequences

Sequence LengthMass (Da)Tools
P78753 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: E561EF981A92FBAC

FASTA55763,241
        10         20         30         40         50         60 
MCGILAVHHV AEDIEAFKPK ALHLSKQLRH RGPDWSGKAI RNQTILCHER LAIVGVESGA 

        70         80         90        100        110        120 
QPLVSDDGKL VLTVNGEIYN HLKLRENLKG NYKFKTYSDC EVILYLYREH GPACANMLDG 

       130        140        150        160        170        180 
MFSWVLYDQD KDKVVAARDP IGITTLYQGF SSDSPDTAYF ASELKALHPV CDKIIAFPPG 

       190        200        210        220        230        240 
HYYDSETKQT VRYFKPSWWD ENKIPSNPVD YKLLRETLEA SVRKRLMAEV PYGVLLSGGL 

       250        260        270        280        290        300 
DSSLIASIAA RETEKLANST SQSEEARTIT AWPKLHSFAI GLPGSPDLLA ARKVADFLHT 

       310        320        330        340        350        360 
FHHEHTFTID EGLDALRDVI YHLETYDVTT IRASTPMYLL SRKIKAQGVK MVLSGEGSDE 

       370        380        390        400        410        420 
IFGGYLYFGN APSREAFHSE CVRRVKNLHL SDCLRANKST MAWGLEARVP FLDKDFLEVA 

       430        440        450        460        470        480 
LNIDPEEKMY INGRKEKYIL RKAFDTTHDS SLQPYLPQDI LWRQKEQFSD GVGYSWIDAL 

       490        500        510        520        530        540 
KDTAELCISD DEFALPRREW GDDIPTTKEA FWYRKLFDEI FPRQCADTVM RWVPKAEWGC 

       550 
PEDPSGRYQA GHVAALK 

« Hide

References

« Hide 'large scale' references
[1]"Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: PR745.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Six new amino acid-auxotrophic markers for targeted gene integration and disruption in fission yeast."
Ma Y., Sugiura R., Saito M., Koike A., Sio S.O., Fujita Y., Takegawa K., Kuno T.
Curr. Genet. 52:97-105(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[5]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391 AND SER-489, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D89101 mRNA. Translation: BAA13764.1. Frameshift.
CU329671 Genomic DNA. Translation: CAA17925.1.
PIRT39308.
RefSeqNP_595291.1. NM_001021198.2.

3D structure databases

ProteinModelPortalP78753.
SMRP78753. Positions 1-529.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid276257. 2 interactions.
MINTMINT-4691132.
STRING4896.SPBC119.10-1.

Protein family/group databases

MEROPSC44.976.

Proteomic databases

MaxQBP78753.
PaxDbP78753.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC119.10.1; SPBC119.10.1:pep; SPBC119.10.
GeneID2539704.
KEGGspo:SPBC119.10.

Organism-specific databases

PomBaseSPBC119.10.

Phylogenomic databases

eggNOGCOG0367.
HOGENOMHOG000027493.
KOK01953.
OMAYWYRLKF.
OrthoDBEOG73JM46.
PhylomeDBP78753.

Enzyme and pathway databases

UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMSSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20800856.
PROP78753.

Entry information

Entry nameASNS_SCHPO
AccessionPrimary (citable) accession number: P78753
Secondary accession number(s): O42902
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways