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P78753

- ASNS_SCHPO

UniProt

P78753 - ASNS_SCHPO

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Protein
Probable asparagine synthetase [glutamine-hydrolyzing]
Gene
asn1, SPBC119.10
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Binding sitei99 – 991Glutamine By similarity
Binding sitei235 – 2351ATP; via carbonyl oxygen By similarity
Binding sitei280 – 2801ATP; via amide nitrogen and carbonyl oxygen By similarity
Sitei356 – 3561Important for beta-aspartyl-AMP intermediate formation By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi354 – 3552ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. asparagine synthase (glutamine-hydrolyzing) activity Source: PomBase

GO - Biological processi

  1. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
  2. asparagine biosynthetic process Source: PomBase
  3. glutamine metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_188302. ATF4 activates genes.
REACT_221562. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:asn1
ORF Names:SPBC119.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC119.10.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Causes asparagine auxotrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 557556Probable asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056916Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei391 – 3911Phosphoserine1 Publication
Modified residuei489 – 4891Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP78753.
PaxDbiP78753.

Interactioni

Protein-protein interaction databases

BioGridi276257. 2 interactions.
MINTiMINT-4691132.
STRINGi4896.SPBC119.10-1.

Structurei

3D structure databases

ProteinModelPortaliP78753.
SMRiP78753. Positions 1-529.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 188187Glutamine amidotransferase type-2
Add
BLAST
Domaini196 – 531336Asparagine synthetase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine binding By similarity
Regioni75 – 773Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
HOGENOMiHOG000027493.
KOiK01953.
OMAiYWYRLKF.
OrthoDBiEOG73JM46.
PhylomeDBiP78753.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P78753-1 [UniParc]FASTAAdd to Basket

« Hide

MCGILAVHHV AEDIEAFKPK ALHLSKQLRH RGPDWSGKAI RNQTILCHER    50
LAIVGVESGA QPLVSDDGKL VLTVNGEIYN HLKLRENLKG NYKFKTYSDC 100
EVILYLYREH GPACANMLDG MFSWVLYDQD KDKVVAARDP IGITTLYQGF 150
SSDSPDTAYF ASELKALHPV CDKIIAFPPG HYYDSETKQT VRYFKPSWWD 200
ENKIPSNPVD YKLLRETLEA SVRKRLMAEV PYGVLLSGGL DSSLIASIAA 250
RETEKLANST SQSEEARTIT AWPKLHSFAI GLPGSPDLLA ARKVADFLHT 300
FHHEHTFTID EGLDALRDVI YHLETYDVTT IRASTPMYLL SRKIKAQGVK 350
MVLSGEGSDE IFGGYLYFGN APSREAFHSE CVRRVKNLHL SDCLRANKST 400
MAWGLEARVP FLDKDFLEVA LNIDPEEKMY INGRKEKYIL RKAFDTTHDS 450
SLQPYLPQDI LWRQKEQFSD GVGYSWIDAL KDTAELCISD DEFALPRREW 500
GDDIPTTKEA FWYRKLFDEI FPRQCADTVM RWVPKAEWGC PEDPSGRYQA 550
GHVAALK 557
Length:557
Mass (Da):63,241
Last modified:January 23, 2007 - v3
Checksum:iE561EF981A92FBAC
GO

Sequence cautioni

The sequence BAA13764.1 differs from that shown. Reason: Frameshift at position 313.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D89101 mRNA. Translation: BAA13764.1. Frameshift.
CU329671 Genomic DNA. Translation: CAA17925.1.
PIRiT39308.
RefSeqiNP_595291.1. NM_001021198.2.

Genome annotation databases

EnsemblFungiiSPBC119.10.1; SPBC119.10.1:pep; SPBC119.10.
GeneIDi2539704.
KEGGispo:SPBC119.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D89101 mRNA. Translation: BAA13764.1 . Frameshift.
CU329671 Genomic DNA. Translation: CAA17925.1 .
PIRi T39308.
RefSeqi NP_595291.1. NM_001021198.2.

3D structure databases

ProteinModelPortali P78753.
SMRi P78753. Positions 1-529.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276257. 2 interactions.
MINTi MINT-4691132.
STRINGi 4896.SPBC119.10-1.

Protein family/group databases

MEROPSi C44.976.

Proteomic databases

MaxQBi P78753.
PaxDbi P78753.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC119.10.1 ; SPBC119.10.1:pep ; SPBC119.10 .
GeneIDi 2539704.
KEGGi spo:SPBC119.10.

Organism-specific databases

PomBasei SPBC119.10.

Phylogenomic databases

eggNOGi COG0367.
HOGENOMi HOG000027493.
KOi K01953.
OMAi YWYRLKF.
OrthoDBi EOG73JM46.
PhylomeDBi P78753.

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .
Reactomei REACT_188302. ATF4 activates genes.
REACT_221562. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

NextBioi 20800856.
PROi P78753.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: PR745.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. "Six new amino acid-auxotrophic markers for targeted gene integration and disruption in fission yeast."
    Ma Y., Sugiura R., Saito M., Koike A., Sio S.O., Fujita Y., Takegawa K., Kuno T.
    Curr. Genet. 52:97-105(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391 AND SER-489, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiASNS_SCHPO
AccessioniPrimary (citable) accession number: P78753
Secondary accession number(s): O42902
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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