P78746 (CHSD_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase D
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase D
Class-VI chitin synthase D

Gene names

Name:	chsD
ORF Names:	AfA5C5.045, AFUA_1G12600

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	745 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class VI subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 745	745	Chitin synthase D		PRO_0000193681
Regions
	Transmembrane	26 – 46	21	Helical; Potential
	Transmembrane	55 – 75	21	Helical; Potential
	Transmembrane	412 – 432	21	Helical; Potential
	Transmembrane	434 – 454	21	Helical; Potential
	Transmembrane	464 – 484	21	Helical; Potential
Experimental info
	Sequence conflict	10 – 29	20	Missing in EAL90592. Ref.3
	Sequence conflict	16 – 20	5	Missing in CAF31978. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P78746 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: B43B5D2B2D4A9632	FASTA	745	84,522
	10 20 30 40 50 60 MIVLFTLLRW APISPVFSMR TMHANLAHRG IFLPVMIVTL PLPVHLRRRF PAQMVLMLQW 70 80 90 100 110 120 FAFGMFSVLL IIPWLLCVYR LVTHSPGRTK RIKQVLDDRT APKTVVVMPV YKEAPETLIR 130 140 150 160 170 180 AIDSVVDCDY PANCIHVFLS YDGCLIDESY LRLIEHLGIP ITLESYPQSI DVTYKDARIT 190 200 210 220 230 240 VSRFKHGGKR HCQKQTFRLI DMVYADYLER HDNLFVLFID SDCILDRVCL QNFMYDMELK 250 260 270 280 290 300 PGSKHDMLAM TGVITSTTDR GSLLTLLQDM EYVHGQLFER SVESSCGAVT CLPGALTMLR 310 320 330 340 350 360 FSAFRKMAKY YFADKAEQCE DFFDYGKCHL GEDRWLTHLF MVGARKRYQI QMCAGAFCKT 370 380 390 400 410 420 EAVQTFSSLL KQRRRWFLGF ITNEVCMLTD VRLWKRYPLL CLVRFMQNTI RTTALLFFII 430 440 450 460 470 480 ALSLITTSSS INDLPVGFIA ISLGLNYVLM FYLGAKLKRY KAWLFPLMFI LNPFFNWLYM 490 500 510 520 530 540 VYGILTAGQR TWGGPRADAA TADEHTSPEE AVELAKAQGD ELNVDLTTFR SRGDEKSVPI 550 560 570 580 590 600 HPSEKIDGRF SAPELPDGYD SNLNDSNAAL TELMTPLPSV PRIGIHTYPS SDSILTSDSL 610 620 630 640 650 660 SSIHLPLKVE ELTGDNDNMK PYPDRQPRDT SSLHQMQRTC SNGIVASDSC SSQDDASEMV 670 680 690 700 710 720 NKPEILSPSA HILPHPSQAT ESSSGEDIYP LHLPSPHQHE AHFAPLNAST RGSMEGNTPE 730 740 VQRPRRKLPG IPRPIRAQKD PESMV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of chsD, a chitin synthase-like gene of Aspergillus fumigatus." Mellado E., Specht C.A., Robbins P.W., Holden D.W. FEMS Microbiol. Lett. 143:69-76(1996) [PubMed: 8807804] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: H237.
[2]	"Insight into the genome of Aspergillus fumigatus: analysis of a 922 kb region encompassing the nitrate assimilation gene cluster." Pain A., Woodward J.R., Quail M.A., Anderson M.J., Clark R., Collins M., Fosker N., Fraser A., Harris D.E., Larke N., Murphy L.D., Humphray S., O'Neil S., Pertea M., Price C., Rabbinowitsch E., Rajandream M.A., Salzberg S.L. , Saunders D., Seeger K., Sharp S., Warren T., Denning D.W., Barrell B.G., Hall N. Fungal Genet. Biol. 41:443-453(2004) [PubMed: 14998527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
[3]	"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U62614 Genomic DNA. Translation: AAB60781.1. BX649606 Genomic DNA. Translation: CAF31978.1. AAHF01000004 Genomic DNA. Translation: EAL90592.1.
RefSeq	XP_752630.1. XM_747537.1.
3D structure databases
ProteinModelPortal	P78746.
ModBase	Search...
Protein-protein interaction databases
STRING	P78746.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3510460.
GenomeReviews	Gene locus chsD in contig CM000169_GR.
KEGG	afm:AFUA_1G12600.
Phylogenomic databases
eggNOG	fuNOG06257.
GeneTree	EFGT00050000001909.
HOGENOM	HBG333467.
OrthoDB	EOG4HTD1B.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. [Graphical view]
KO	K00698.
Pfam	PF03142. Chitin_synth_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHSD_ASPFU

Accession

Primary (citable) accession number: P78746
Secondary accession number(s): Q4WSJ0, Q6MYM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1997
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program