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Protein

Manganese peroxidase H3

Gene
N/A
Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of Mn2+ to Mn3+. The latter, acting as a diffusible redox mediator, is capable of oxidizing a variety of lignin compounds.

Catalytic activityi

2 Mn2+ + 2 H+ + H2O2 = 2 Mn3+ + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60ManganeseBy similarity1
Metal bindingi64ManganeseBy similarity1
Sitei67Transition state stabilizerPROSITE-ProRule annotation1
Active sitei71Proton acceptorPROSITE-ProRule annotation1
Metal bindingi72Calcium 1PROSITE-ProRule annotation1
Metal bindingi86Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi88Calcium 1PROSITE-ProRule annotation1
Metal bindingi90Calcium 1PROSITE-ProRule annotation1
Metal bindingi197Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi198Calcium 2PROSITE-ProRule annotation1
Metal bindingi203ManganeseBy similarity1
Metal bindingi215Calcium 2PROSITE-ProRule annotation1
Metal bindingi217Calcium 2PROSITE-ProRule annotation1
Metal bindingi220Calcium 2; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi222Calcium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide, Lignin degradation

Keywords - Ligandi

Calcium, Heme, Iron, Manganese, Metal-binding

Protein family/group databases

CAZyiAA2. Auxiliary Activities 2.
mycoCLAPiMPO2C_PHACH.
PeroxiBasei2382. PcMnP03_.

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese peroxidase H3 (EC:1.11.1.13)
Alternative name(s):
Peroxidase manganese-dependent H3
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000002377926 – 380Manganese peroxidase H3Add BLAST355

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 40PROSITE-ProRule annotation
Disulfide bondi39 ↔ 312PROSITE-ProRule annotation
Disulfide bondi58 ↔ 141PROSITE-ProRule annotation
Glycosylationi100N-linked (GlcNAc...)Sequence analysis1
Glycosylationi155N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi277 ↔ 342PROSITE-ProRule annotation
Disulfide bondi364 ↔ 371PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP78733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Ligninase subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZ9A. Eukaryota.
ENOG410YEPY. LUCA.

Family and domain databases

CDDicd00692. ligninase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR001621. Ligninase.
IPR024589. Ligninase_C.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
PF11895. Peroxidase_ext. 1 hit.
[Graphical view]
PRINTSiPR00462. LIGNINASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P78733-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFASSLLAL VALAAVTSAA PATTQATCPD GTKVNNAACC AFIPLAQDLQ
60 70 80 90 100
ETIFQNDCGE DAHEVIRLTF HDAIAISQSK GPSAGGADGS MLLFPTIEPN
110 120 130 140 150
FSANNGIDDS VNNLIPFMQK HNTISAGDIV QFTGAVALTN CPGAPQLEFL
160 170 180 190 200
ARRPNKTIPA IDGLIPEPQD SVTSILERFK DAGNFSPFEV VSLLASHSVA
210 220 230 240 250
RADKVDETID AAPFDTTPFV FDTQIFLEVL LKGVGFPGTR TTRGEVASPL
260 270 280 290 300
PLTSGSDTGE LRLQSDFALA RDERTACIWQ GFVNEQALMA SFKAAMRKLA
310 320 330 340 350
VLGQHRNTLI DCSDVVPAPK PAVNKPASFP ATTGPQDLEL SCNTKPFPSL
360 370 380
SVDAGAQQTL IPHCSDGDMT CQSVQFNGPA
Length:380
Mass (Da):40,078
Last modified:July 5, 2004 - v2
Checksum:i4C8931EA3940BBA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10306 mRNA. Translation: AAA62243.1.
PIRiJC2579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10306 mRNA. Translation: AAA62243.1.
PIRiJC2579.

3D structure databases

ProteinModelPortaliP78733.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiAA2. Auxiliary Activities 2.
mycoCLAPiMPO2C_PHACH.
PeroxiBasei2382. PcMnP03_.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IZ9A. Eukaryota.
ENOG410YEPY. LUCA.

Family and domain databases

CDDicd00692. ligninase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR001621. Ligninase.
IPR024589. Ligninase_C.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
PF11895. Peroxidase_ext. 1 hit.
[Graphical view]
PRINTSiPR00462. LIGNINASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEM3_PHACH
AccessioniPrimary (citable) accession number: P78733
Secondary accession number(s): Q01666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.