Reviewed, UniProtKB/Swiss-Prot P78717 (UBC2_NECHA)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 2
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase UBC2
    Ubiquitin carrier protein UBC2
    Radiation sensitivity protein 6

Gene names

Name:	UBC2
Synonyms:	RAD6

Organism

Nectria haematococca

Taxonomic identifier

140110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Nectria › Nectria haematococca complex

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. Also involved in postreplication repair of UV-damaged DNA, in N-end rule-dependent protein degradation and in sporulation By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	CytoplasmBy similarity. NucleusBy similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Sporulation Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Molecular function	Chromatin regulator Ligase
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Ubiquitin-conjugating enzyme E2 2		PRO_0000082534
Sites
	Active site	88	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P78717-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 157024A4EA568CCF	FASTA	151	17,200
	10 20 30 40 50 60 MSTAARRRLM RDFKRMQTDP PAGVSASPVP DNVMTWNAVI IGPADTPFED GTFRLVMQFE 70 80 90 100 110 120 EQYPNKPPQV KFISEMFHPN VYATGELCLD ILQNRWSPTY DVAAVLTSIQ SLLNDPNTGS 130 140 150 PANVEASNLY KDNRKEYTKR VRETVEKSWE D

References

[1]	"A fungal kinesin required for organelle motility, hyphal growth, and morphogenesis." Wu Q., Sandrock T.M., Turgeon B.G., Yoder O.C., Wirsel S.G., Aist J.R. Mol. Biol. Cell 9:89-101(1998) [PubMed: 9436993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: T213.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U86521 Genomic DNA. Translation: AAB47850.1.
PIR	T51931.
3D structure databases
HSSP	HSSP built from PDB template 1AYZ based on UniProtKB P06104.
SMR	P78717. Positions 2-151.
ModBase	Search...
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PANTHER	PTHR11621. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC2_NECHA

Accession

Primary (citable) accession number: P78717

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	May 1, 1997
Last modified:	November 25, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)