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Protein

Ubiquitin-conjugating enzyme E2 2

Gene

UBC2

Organism
Nectria haematococca
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. Also involved in postreplication repair of UV-damaged DNA, in N-end rule-dependent protein degradation and in sporulation (By similarity).PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: EnsemblFungi

GO - Biological processi

  1. chromatin silencing at telomere Source: EnsemblFungi
  2. double-strand break repair via homologous recombination Source: EnsemblFungi
  3. ER-associated ubiquitin-dependent protein catabolic process Source: EnsemblFungi
  4. error-free postreplication DNA repair Source: EnsemblFungi
  5. error-free translesion synthesis Source: EnsemblFungi
  6. error-prone translesion synthesis Source: EnsemblFungi
  7. histone monoubiquitination Source: EnsemblFungi
  8. meiotic DNA double-strand break formation Source: EnsemblFungi
  9. mitotic G1 DNA damage checkpoint Source: EnsemblFungi
  10. protein polyubiquitination Source: EnsemblFungi
  11. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: EnsemblFungi
  12. regulation of dipeptide transport Source: EnsemblFungi
  13. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  14. transcription, DNA-templated Source: UniProtKB-KW
  15. ubiquitin-dependent protein catabolic process via the N-end rule pathway Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Ligase

Keywords - Biological processi

DNA damage, DNA repair, Sporulation, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 2 (EC:6.3.2.19)
Alternative name(s):
Radiation sensitivity protein 6
Ubiquitin carrier protein UBC2
Ubiquitin-protein ligase UBC2
Gene namesi
Name:UBC2
Synonyms:RAD6
OrganismiNectria haematococca
Taxonomic identifieri140110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium solani species complex

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. Rad6-Rad18 complex Source: EnsemblFungi
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 151151Ubiquitin-conjugating enzyme E2 2PRO_0000082534Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP78717.
SMRiP78717. Positions 2-151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

OMAiPVPDNVM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78717-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTAARRRLM RDFKRMQTDP PAGVSASPVP DNVMTWNAVI IGPADTPFED
60 70 80 90 100
GTFRLVMQFE EQYPNKPPQV KFISEMFHPN VYATGELCLD ILQNRWSPTY
110 120 130 140 150
DVAAVLTSIQ SLLNDPNTGS PANVEASNLY KDNRKEYTKR VRETVEKSWE

D
Length:151
Mass (Da):17,200
Last modified:May 1, 1997 - v1
Checksum:i157024A4EA568CCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86521 Genomic DNA. Translation: AAB47850.1.
PIRiT51931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86521 Genomic DNA. Translation: AAB47850.1.
PIRiT51931.

3D structure databases

ProteinModelPortaliP78717.
SMRiP78717. Positions 2-151.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OMAiPVPDNVM.

Enzyme and pathway databases

UniPathwayiUPA00143.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A fungal kinesin required for organelle motility, hyphal growth, and morphogenesis."
    Wu Q., Sandrock T.M., Turgeon B.G., Yoder O.C., Wirsel S.G., Aist J.R.
    Mol. Biol. Cell 9:89-101(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: T213.

Entry informationi

Entry nameiUBC2_NECHA
AccessioniPrimary (citable) accession number: P78717
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: May 1, 1997
Last modified: April 1, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.