P78710 (ASD1_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative rhamnogalacturonase
EC=4.2.2.-

Alternative name(s):
Ascus development protein 1
Short name=Asd-I
Rhamnogalacturonan lyase
Short name=RGase

Gene names

Name:	asd-1
ORF Names:	NCU05598

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Could be a pectinolytic enzyme that hydrolyzes the alpha-L-rhamnopyranosyl-(1,4)-alpha-D-galacturonopyranosyl glycosidic linkage by beta-elimination, thereby generating oligosaccharides terminating at the non-reducing end with a hex-4-enopyranosyluronic acid residue.
Developmental stage	Expressed preferentially during the sexual cycle and essential for normal sexual development.
Sequence similarities	Belongs to the polysaccharide lyase 4 family.
Sequence caution	The sequence EAA31324.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Signal
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro carbon-oxygen lyase activity, acting on polysaccharides Inferred from electronic annotation. Source: InterPro carboxypeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 540	517	Putative rhamnogalacturonase		PRO_0000024913
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	368	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P78710 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: B4638AA1B93A28A0	FASTA	540	58,011
	10 20 30 40 50 60 MGFLTLFHMA FLAVSLFVSG ALAAFGYTTS GNNFVIDAGS ANPLIFSVSK SSCDINSIRY 70 80 90 100 110 120 RGTELQYSKQ GSHIGSGLGK ATVSVSQING SKSKFIKVTC VTSTLTQYMI VKEADSTIYM 130 140 150 160 170 180 ATYITAEPAI GELRFIARLL SDKLPYEYPY GEVSTTKGSS STVEGSDVFV VNGQTRSKFY 190 200 210 220 230 240 SSTRFIDEDS HCVYGGSDLT HVCIITPQQE SSSGGPFFRD IDSNNAGEST NLYNYMNSGH 250 260 270 280 290 300 VQTEDRRMGL HGPYLMTFSR SGIPKLKTVD ISWFGELGVT GYVPDSQRGT VIGRATGIPS 310 320 330 340 350 360 GFEGVVHWYN AAAQYWVRTA PNGDFTSPKM KPGTYTMVLY QTEFKVATST VTVSAGKTTT 370 380 390 400 410 420 ASIASTFNTS HTTLFKIGEY DGQPTGFRNA DKFLRMHPSD SRMSSWGPLT YTVGSSSLND 430 440 450 460 470 480 FPMAVFKSVN NPVTIKFNLG SAPSQATTLR IATTLSFAGA RPQVVVNGWS APAPAAPAKI 490 500 510 520 530 540 DSRGVTRGAY RGYGEVYDVA VPAGKLISGT NTITISALSG SSGATFLSPN FIFDAVELFY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A putative rhamnogalacturonase required for sexual development of Neurospora crassa." Nelson M.A., Merino S.T., Metzenberg R.L. Genetics 146:531-540(1997) [PubMed: 9178004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U70861 Genomic DNA. Translation: AAB39649.1. AABX02000022 Genomic DNA. Translation: EAA31324.2. Sequence problems.
RefSeq	XP_960560.2. XM_955467.2.
UniGene	Ncr.20951.
3D structure databases
ProteinModelPortal	P78710.
SMR	P78710. Positions 24-539.
ModBase	Search...
Protein family/group databases
CAZy	PL4. Polysaccharide Lyase Family 4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000005603; EFNCRP00000005597; EFNCRG00000005593.
GeneID	3876675.
KEGG	ncr:NCU05598.
NMPDR	fig|5141.1.peg.3386.
Phylogenomic databases
eggNOG	fuNOG06038.
GeneTree	EFGT00050000001923.
OrthoDB	EOG4F7RT8.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-009346-MONOMER.
Family and domain databases
InterPro	IPR013784. Carb-bd-like_fold. IPR014766. CarboxyPept_regulatory_dom. IPR008979. Galactose-bd-like. IPR011013. Glyco_hydro-type_carb-bd. IPR016590. Rhamnogalacturonase_B. IPR015364. RhgB_N. [Graphical view]
Gene3D	G3DSA:2.60.40.1120. CarboxyPept_regulatory. 1 hit.
Pfam	PF09284. RhgB_N. 1 hit. [Graphical view]
PIRSF	PIRSF011794. Rhamnogalacturonase_B. 1 hit.
SUPFAM	SSF49452. CBD_4. 1 hit. SSF49785. Gal_bind_like. 1 hit. SSF74650. Gal_mut_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASD1_NEUCR

Accession

Primary (citable) accession number: P78710
Secondary accession number(s): Q7RVB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	October 19, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program