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Protein

Putative rhamnogalacturonase

Gene

asd-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Could be a pectinolytic enzyme that hydrolyzes the alpha-L-rhamnopyranosyl-(1,4)-alpha-D-galacturonopyranosyl glycosidic linkage by beta-elimination, thereby generating oligosaccharides terminating at the non-reducing end with a hex-4-enopyranosyluronic acid residue.

Catalytic activityi

Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Protein family/group databases

CAZyiPL4. Polysaccharide Lyase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative rhamnogalacturonase (EC:4.2.2.23)
Alternative name(s):
Ascus development protein 1
Short name:
Asd-I
Rhamnogalacturonan lyase
Short name:
RGase
Gene namesi
Name:asd-1
ORF Names:NCU05598
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 5, Linkage Group VI

Organism-specific databases

EuPathDBiFungiDB:NCU05598.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 540517Putative rhamnogalacturonasePRO_0000024913Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 100By similarity
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence analysis
Disulfide bondi192 ↔ 203By similarity
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

Expressed preferentially during the sexual cycle and essential for normal sexual development.

Structurei

3D structure databases

ProteinModelPortaliP78710.
SMRiP78710. Positions 24-539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide lyase 4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000171008.
InParanoidiP78710.
KOiK18195.
OrthoDBiEOG092C31LY.

Family and domain databases

CDDicd10316. RGL4_M. 1 hit.
Gene3Di2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
2.70.98.10. 1 hit.
InterProiIPR013784. Carb-bd-like_fold.
IPR014766. CarboxyPept_regulatory_dom.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR029413. RG-lyase_II.
IPR029411. RG-lyase_III.
IPR016590. Rhamnogalacturonase_B.
IPR015364. RhgB_N.
[Graphical view]
PfamiPF14683. CBM-like. 1 hit.
PF14686. fn3_3. 1 hit.
PF09284. RhgB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF011794. Rhamnogalacturonase_B. 1 hit.
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF74650. SSF74650. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P78710-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLTLFHMA FLAVSLFVSG ALAAFGYTTS GNNFVIDAGS ANPLIFSVSK
60 70 80 90 100
SSCDINSIRY RGTELQYSKQ GSHIGSGLGK ATVSVSQING SKSKFIKVTC
110 120 130 140 150
VTSTLTQYMI VKEADSTIYM ATYITAEPAI GELRFIARLL SDKLPYEYPY
160 170 180 190 200
GEVSTTKGSS STVEGSDVFV VNGQTRSKFY SSTRFIDEDS HCVYGGSDLT
210 220 230 240 250
HVCIITPQQE SSSGGPFFRD IDSNNAGEST NLYNYMNSGH VQTEDRRMGL
260 270 280 290 300
HGPYLMTFSR SGIPKLKTVD ISWFGELGVT GYVPDSQRGT VIGRATGIPS
310 320 330 340 350
GFEGVVHWYN AAAQYWVRTA PNGDFTSPKM KPGTYTMVLY QTEFKVATST
360 370 380 390 400
VTVSAGKTTT ASIASTFNTS HTTLFKIGEY DGQPTGFRNA DKFLRMHPSD
410 420 430 440 450
SRMSSWGPLT YTVGSSSLND FPMAVFKSVN NPVTIKFNLG SAPSQATTLR
460 470 480 490 500
IATTLSFAGA RPQVVVNGWS APAPAAPAKI DSRGVTRGAY RGYGEVYDVA
510 520 530 540
VPAGKLISGT NTITISALSG SSGATFLSPN FIFDAVELFY
Length:540
Mass (Da):58,011
Last modified:May 1, 1997 - v1
Checksum:iB4638AA1B93A28A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70861 Genomic DNA. Translation: AAB39649.1.
CM002241 Genomic DNA. Translation: EAA31324.3.
RefSeqiXP_960560.3. XM_955467.3.

Genome annotation databases

EnsemblFungiiEFNCRT00000005603; EFNCRP00000005597; EFNCRG00000005593.
GeneIDi3876675.
KEGGincr:NCU05598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70861 Genomic DNA. Translation: AAB39649.1.
CM002241 Genomic DNA. Translation: EAA31324.3.
RefSeqiXP_960560.3. XM_955467.3.

3D structure databases

ProteinModelPortaliP78710.
SMRiP78710. Positions 24-539.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiPL4. Polysaccharide Lyase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000005603; EFNCRP00000005597; EFNCRG00000005593.
GeneIDi3876675.
KEGGincr:NCU05598.

Organism-specific databases

EuPathDBiFungiDB:NCU05598.

Phylogenomic databases

HOGENOMiHOG000171008.
InParanoidiP78710.
KOiK18195.
OrthoDBiEOG092C31LY.

Family and domain databases

CDDicd10316. RGL4_M. 1 hit.
Gene3Di2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
2.70.98.10. 1 hit.
InterProiIPR013784. Carb-bd-like_fold.
IPR014766. CarboxyPept_regulatory_dom.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR029413. RG-lyase_II.
IPR029411. RG-lyase_III.
IPR016590. Rhamnogalacturonase_B.
IPR015364. RhgB_N.
[Graphical view]
PfamiPF14683. CBM-like. 1 hit.
PF14686. fn3_3. 1 hit.
PF09284. RhgB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF011794. Rhamnogalacturonase_B. 1 hit.
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF74650. SSF74650. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASD1_NEUCR
AccessioniPrimary (citable) accession number: P78710
Secondary accession number(s): Q7RVB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.