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Protein

5-aminolevulinate synthase, mitochondrial

Gene

HEM1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of 5-aminolevulinate (ALA) from succinyl-CoA and glycine, the first and rate-limiting step in heme biosynthesis.By similarity

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.By similarity

Cofactori

pyridoxal 5'-phosphateBy similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase, mitochondrial (HEM1)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119SubstrateBy similarity1
Binding sitei232SubstrateBy similarity1
Binding sitei251SubstrateBy similarity1
Binding sitei284Pyridoxal phosphateBy similarity1
Binding sitei312Pyridoxal phosphateBy similarity1
Binding sitei356Pyridoxal phosphateBy similarity1
Active sitei359By similarity1
Binding sitei388Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei389Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei474SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processHeme biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, mitochondrial (EC:2.3.1.37)
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene namesi
Name:HEM1
Ordered Locus Names:KLLA0C15059g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome C

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53MitochondrionSequence analysisAdd BLAST53
ChainiPRO_000000124154 – 5705-aminolevulinate synthase, mitochondrialAdd BLAST517

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei359N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PRIDEiP78698

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi284590.XP_452875.1

Structurei

3D structure databases

ProteinModelPortaliP78698
SMRiP78698
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1360 Eukaryota
COG0156 LUCA
HOGENOMiHOG000221020
InParanoidiP78698
KOiK00643
OMAiKQDHTYR
OrthoDBiEOG092C1OK6

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR010961 4pyrrol_synth_NH2levulA_synth
IPR001917 Aminotrans_II_pyridoxalP_BS
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01821 5aminolev_synth, 1 hit
PROSITEiView protein in PROSITE
PS00599 AA_TRANSFER_CLASS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P78698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESVIRSSAK ICPFMHSATG SMQSVKALKN ANLPAIAQQC PFMGKAMEQR
60 70 80 90 100
RGYASSASGA SAAAAATATA STSASNSNSS VEASASADVV DHATKEASFD
110 120 130 140 150
YQGLFDSDLA KKRMDKSYRF FNNINRLAKE FPMAHRKLED DKVTVWCSND
160 170 180 190 200
YLALSKNQEV IEVMKKTLDK YGAGAGGTRN IAGHNKHALQ LEAELATLHK
210 220 230 240 250
KEGALVFSSC FVANDAVISL LGQKIKDLVI FSDELNHASM IVGIKHASTK
260 270 280 290 300
KHIFKHNNLD QLEELLAMYP KSTPKLIAFE SVYSMSGSVA DIDKICDLAE
310 320 330 340 350
KYGALTFLDE VHAVGLYGPH GAGVAEHCNF DAHRKAGIAS PEFRTVMDRV
360 370 380 390 400
DMITGTLGKS FGTVGGYVAG SLQLIDWVRS YAPGFIFTTT LPPAVMAGAA
410 420 430 440 450
EAIRYQRSHL DLRQDQQRHT TYVKDGLADL GIPVMPNPSH IVPVLVGNPH
460 470 480 490 500
LAKQASDILM DKHRIYVQAI NFPTVARGTE RLRITPTPGH TNDLSDILMD
510 520 530 540 550
ALEDVWSTLQ LPRVRDWEAQ GGLLGVGDPN HVPQPNLWTK DQLTLTNNDL
560 570
HPNVKQPIIE QLEVSSGIRY
Length:570
Mass (Da):62,139
Last modified:September 27, 2004 - v2
Checksum:iCD9151C4A529AA89
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti332A → R in CAA63497 (PubMed:9271110).Curated1
Sequence conflicti487T → S in CAA63497 (PubMed:9271110).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92944 Genomic DNA Translation: CAA63497.1
CR382123 Genomic DNA Translation: CAH01726.1
RefSeqiXP_452875.1, XM_452875.1

Genome annotation databases

EnsemblFungiiCAH01726; CAH01726; KLLA0_C15059g
GeneIDi2892616
KEGGikla:KLLA0C15059g

Similar proteinsi

Entry informationi

Entry nameiHEM1_KLULA
AccessioniPrimary (citable) accession number: P78698
Secondary accession number(s): Q6CT64
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 27, 2004
Last modified: May 23, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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