Reviewed, UniProtKB/Swiss-Prot P78698 (HEM1_KLULA)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase, mitochondrial
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthase

Gene names

Name:	HEM1
Ordered Locus Names:	KLLA0C15059g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 570		5-aminolevulinate synthase, mitochondrial		PRO_0000001241
Amino acid modifications
	Modified residue	359	1	N6-(pyridoxal phosphate)lysine Probable
Experimental info
	Sequence conflict	332	1	A → R in CAA63497. Ref.1
	Sequence conflict	487	1	T → S in CAA63497. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P78698-1 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: CD9151C4A529AA89	FASTA	570	62,139
	10 20 30 40 50 60 MESVIRSSAK ICPFMHSATG SMQSVKALKN ANLPAIAQQC PFMGKAMEQR RGYASSASGA 70 80 90 100 110 120 SAAAAATATA STSASNSNSS VEASASADVV DHATKEASFD YQGLFDSDLA KKRMDKSYRF 130 140 150 160 170 180 FNNINRLAKE FPMAHRKLED DKVTVWCSND YLALSKNQEV IEVMKKTLDK YGAGAGGTRN 190 200 210 220 230 240 IAGHNKHALQ LEAELATLHK KEGALVFSSC FVANDAVISL LGQKIKDLVI FSDELNHASM 250 260 270 280 290 300 IVGIKHASTK KHIFKHNNLD QLEELLAMYP KSTPKLIAFE SVYSMSGSVA DIDKICDLAE 310 320 330 340 350 360 KYGALTFLDE VHAVGLYGPH GAGVAEHCNF DAHRKAGIAS PEFRTVMDRV DMITGTLGKS 370 380 390 400 410 420 FGTVGGYVAG SLQLIDWVRS YAPGFIFTTT LPPAVMAGAA EAIRYQRSHL DLRQDQQRHT 430 440 450 460 470 480 TYVKDGLADL GIPVMPNPSH IVPVLVGNPH LAKQASDILM DKHRIYVQAI NFPTVARGTE 490 500 510 520 530 540 RLRITPTPGH TNDLSDILMD ALEDVWSTLQ LPRVRDWEAQ GGLLGVGDPN HVPQPNLWTK 550 560 570 DQLTLTNNDL HPNVKQPIIE QLEVSSGIRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the KlHEM1 gene in Kluyveromyces lactis." Gonzales-Dominguez M., Mendez-Carro C., Cerdan M.E. Yeast 13:961-971(1997) [PubMed: 9271110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92944 Genomic DNA. Translation: CAA63497.1. CR382123 Genomic DNA. Translation: CAH01726.1.
RefSeq	XP_452875.1.
3D structure databases
SMR	P78698. Positions 100-506.
ModBase	Search...
Protein-protein interaction databases
STRING	P78698.
Genome annotation databases
GeneID	2892616.
GenomeReviews	Gene locus KLLA0C15059g in contig CR382123_GR.
KEGG	kla:KLLA0C15059g.
Phylogenomic databases
eggNOG	fuNOG07159.
HOGENOM	HBG649839.
OMA	HLKESNE.
OrthoDB	EOG9CVHQ2.
PhylomeDB	P78698.
Enzyme and pathway databases
BRENDA	2.3.1.37. 74088.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_KLULA

Accession

Primary (citable) accession number: P78698
Secondary accession number(s): Q6CT64

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 27, 2004
Last modified:	February 9, 2010
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)