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P78621

- SEPA_EMENI

UniProt

P78621 - SEPA_EMENI

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Protein
Cytokinesis protein sepA
Gene
sepA, figA, AN6523
Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Involved in cytokinesis. Overexpression results in growth inhibition.1 Publication

GO - Biological processi

  1. actomyosin contractile ring assembly Source: ASPGD
  2. cell septum assembly Source: ASPGD
  3. cellular response to heat Source: ASPGD
  4. establishment or maintenance of cell polarity Source: ASPGD
  5. hyphal growth Source: ASPGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Names & Taxonomyi

Protein namesi
Recommended name:
Cytokinesis protein sepA
Alternative name(s):
Forced expression inhibition of growth A
Protein FH1/2
Gene namesi
Name:sepA
Synonyms:figA
ORF Names:AN6523
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560: Chromosome I

Subcellular locationi

GO - Cellular componenti

  1. hyphal tip Source: ASPGD
  2. spitzenkorper Source: ASPGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17901790Cytokinesis protein sepA
PRO_0000194903Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP78621.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini274 – 702429GBD/FH3
Add
BLAST
Domaini955 – 1136182FH1
Add
BLAST
Domaini1141 – 1564424FH2
Add
BLAST
Domaini1581 – 161333DAD
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili724 – 81188 Reviewed prediction
Add
BLAST
Coiled coili1435 – 1566132 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1016 – 1117102Pro-rich
Add
BLAST
Compositional biasi1608 – 16114Arg/Lys-rich (basic)

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments By similarity.

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG264408.
HOGENOMiHOG000200715.
KOiK11238.
OrthoDBiEOG7G4QPH.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR014767. Diaphanous_autoregulatory.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
[Graphical view]
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78621-1 [UniParc]FASTAAdd to Basket

« Hide

MPTSDKSRQT SAGKSFFGRK LHKERPVEDR WDAHGSWESL APPSSAAGSR     50
SSRYSKRSSI QSVDFGADID PSLLSTSAGP ITSIPFESLS TDTQSPIPVD 100
YLSKAETSPR KEPSPGHLAK GVGDFHQYPA WDPSAMRNHQ QFSHPTGPRP 150
PPHAAGVAMS SSATGDKGAR YQQWGRPGSS AGNAGLSHHS SSTVDSSTNS 200
RMSIDQASIH SSLSSNTRGS SYISTDGSSR TTLPSHSNDR SNYYAAMNSG 250
RGSSAQGAIP PAQPRVQNTE QYLTRPRDDR VVDQLFLELM QKRGWQNLPE 300
QARRQMMAYP ASKKWTLVHQ DRLTELQGEQ KRKQKARETH GYDGPSGILE 350
RADEEGSPEW YVKKVMDDTI TSKQLASLSV SLRTQPISWV KAFVEAQGQI 400
ALTNVLVKIN RKKVTGPVPA PPSGDKDLDR EYDIVKCLKA LMNNKYGADD 450
ALAHQQIIVA LISSLLSPRL NTRKLVSEVL TFLCHWAEGQ GHERVLQAMD 500
HVKNHQGETG RFDAWMRIVE VTIDGRGKMG SLVGASEEYR SGGIGMENLL 550
MEYAVSTMIL INMLVDAPEN DLQLRCHIRA QFISCGIKRL LSKMEGFQYE 600
VIDKQIEHFR ENEAIDYEDL LQRESSSTKD SIEGEVKDMT DPLQITDAIA 650
SRLNGTRAHD YFLSALQHLL LIRENSGEDG LRMYQLVDAM LSYVAMDRRL 700
PDLDLRQGLT FTVQSLLDRL HTDAEARRAY DESLEARQIA EAALAERDEM 750
KAQVELGADG LVRKLQKQIE EQTGIIELQS RQNEMLKAEL ADVQRLRAQE 800
LQRNELETRE LYLMLRDAQD IAASNAKKSN MGEAETDPAH MRGILDREKL 850
LTRLEKQLER TKTQFKLEGK VWGQHDPSDR LRELREQMDG DAGPREAFEE 900
QARLNLSLNP VGSVYRKKTY IQGMEDTATE ELGQTDDEVV YAKARLVDLH 950
RPRMDPEQAT GLLGEIAAKV PKIDADDAKD EGKPTESEQP AEGAATKGDE 1000
QGVDDTVAVD KATAAPPPPP PPPPAHPGLS GAAPPPPPPP PPPPPGAGAA 1050
PPPPPPPPPP PPGGLGGPPP PPPPPPPGGF GGPPPPPPPP GGFGGPPPPP 1100
PPPPGGAFGV PPPPPPPGTV IGGWRANYLA SQGAPSHAIP VMSSIRPKKK 1150
LKALHWDKVD TPQVTVWATH GTTPQEKEEK YVELAKRGVL DEVERLFMAK 1200
ETRIFGGGVA AKQRKDKKQI ISNDLSKNFQ IALSKFSQFP AEEVVRRIIH 1250
CDAEILDNMV VMEFLQRDEM CTVPENVSKL MAPYSKDWTG PDAANTEREQ 1300
DPSELTREDQ IYLYTAFELN HYWKARMRAL ALTRSFEPDY EHISAKLREV 1350
VRVSESLRDS VSLMNVLGLI LDIGNFMNDA NKQAQGFKLS SLARLGMVKD 1400
DKNETTFADL VERIVRNQYP EWEDFTEQIS GVIGLQKLNV DQLRTDAKKY 1450
IDNIKNVQAS LDAGNLSDPK KFHPQDRVSQ ITQRSMKDAR RKAEQMQLYL 1500
EEMVKTYDDI MVFYGEDNTD DGARRDFFAK LAAFLQEWKK SKEKNIALEE 1550
ARRRTEASLA RKRINVGLAN GAGAAGDAPV SPATSGAMDS LLEKLRAAAP 1600
QAKDQRDRRR RARLKERHQV RVASGQKIPD LEGAEAPGSG GQNSGATDTN 1650
ATDSSLLSPT IQEPEGGSSP IASQSEDVAD RAASMLQDML RNSPDPERTR 1700
RRRESAEEER RKRRLRRRNG ATSGSKDSND TTPLSPVTEP TSTQGESAEP 1750
ENLSLSSPPN GEDPTLNPPT IVLSSDASDT PDDEHRPSTS 1790
Length:1,790
Mass (Da):197,356
Last modified:August 14, 2001 - v2
Checksum:i192136DE2EF2A75B
GO

Sequence cautioni

The sequence CBF70912.1 differs from that shown. Reason: An additional intron is predicted in this sequence to correct a probable sequencing error.
The sequence EAA57863.1 differs from that shown. Reason: An additional intron is predicted in this sequence to correct a probable sequencing error.
The sequence AAA33306.1 differs from that shown. Reason: Frameshift at positions 1071 and 1109.
The sequence AAA33306.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CBF70912.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence EAA57863.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti713 – 7131Missing in EAA57863. 1 Publication
Sequence conflicti713 – 7131Missing in CBF70912. 1 Publication
Sequence conflicti1104 – 11041P → T in AAA33306. 1 Publication
Sequence conflicti1476 – 14761D → V in AAA33306. 1 Publication
Sequence conflicti1504 – 15041V → L in AAA33306. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U83658 Genomic DNA. Translation: AAB63335.3.
AACD01000109 Genomic DNA. Translation: EAA57863.1. Sequence problems.
L36341 Genomic DNA. Translation: AAA33306.1. Sequence problems.
BN001301 Genomic DNA. Translation: CBF70912.1. Sequence problems.
RefSeqiXP_664127.1. XM_659035.1.

Genome annotation databases

EnsemblFungiiCADANIAT00007295; CADANIAP00007295; CADANIAG00007295.
GeneIDi2870211.
KEGGiani:AN6523.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U83658 Genomic DNA. Translation: AAB63335.3 .
AACD01000109 Genomic DNA. Translation: EAA57863.1 . Sequence problems.
L36341 Genomic DNA. Translation: AAA33306.1 . Sequence problems.
BN001301 Genomic DNA. Translation: CBF70912.1 . Sequence problems.
RefSeqi XP_664127.1. XM_659035.1.

3D structure databases

ProteinModelPortali P78621.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANIAT00007295 ; CADANIAP00007295 ; CADANIAG00007295 .
GeneIDi 2870211.
KEGGi ani:AN6523.2.

Phylogenomic databases

eggNOGi NOG264408.
HOGENOMi HOG000200715.
KOi K11238.
OrthoDBi EOG7G4QPH.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR014767. Diaphanous_autoregulatory.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
[Graphical view ]
Pfami PF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view ]
SMARTi SM00498. FH2. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 2 hits.
PROSITEi PS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity."
    Harris S.D., Hamer L., Sharpless K.E., Hamer J.E.
    EMBO J. 16:3474-3483(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Hamer L., Harris S.D., Sharpless K.E., Hamer J.E.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 143-153; 207; 1071-1109 AND 1644.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  4. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  5. "Identification of developmental regulatory genes in Aspergillus nidulans by overexpression."
    Marhoul J.F., Adams T.H.
    Genetics 139:537-547(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 559-1790, FUNCTION.
    Strain: FGSC 26.

Entry informationi

Entry nameiSEPA_EMENI
AccessioniPrimary (citable) accession number: P78621
Secondary accession number(s): C8V0K5, Q00760, Q5AYV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 14, 2001
Last modified: May 14, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi