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P78621 (SEPA_EMENI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytokinesis protein sepA
Alternative name(s):
Forced expression inhibition of growth A
Protein FH1/2
Gene names
Name:sepA
Synonyms:figA
ORF Names:AN6523
OrganismEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]
Taxonomic identifier227321 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length1790 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in cytokinesis. Overexpression results in growth inhibition. Ref.5

Domain

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments By similarity.

Sequence similarities

Belongs to the formin homology family. BNI1 subfamily.

Contains 1 DAD (diaphanous autoregulatory) domain.

Contains 1 FH1 (formin homology 1) domain.

Contains 1 FH2 (formin homology 2) domain.

Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.

Sequence caution

The sequence AAA33306.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAA33306.1 differs from that shown. Reason: Frameshift at positions 1071 and 1109.

The sequence CBF70912.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CBF70912.1 differs from that shown. Reason: An additional intron is predicted in this sequence to correct a probable sequencing error.

The sequence EAA57863.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EAA57863.1 differs from that shown. Reason: An additional intron is predicted in this sequence to correct a probable sequencing error.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17901790Cytokinesis protein sepA
PRO_0000194903

Regions

Domain274 – 702429GBD/FH3
Domain955 – 1136182FH1
Domain1141 – 1564424FH2
Domain1581 – 161333DAD
Coiled coil724 – 81188 Potential
Coiled coil1435 – 1566132 Potential
Compositional bias1016 – 1117102Pro-rich
Compositional bias1608 – 16114Arg/Lys-rich (basic)

Experimental info

Sequence conflict7131Missing in EAA57863. Ref.3
Sequence conflict7131Missing in CBF70912. Ref.3
Sequence conflict11041P → T in AAA33306. Ref.5
Sequence conflict14761D → V in AAA33306. Ref.5
Sequence conflict15041V → L in AAA33306. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P78621 [UniParc].

Last modified August 14, 2001. Version 2.
Checksum: 192136DE2EF2A75B

FASTA1,790197,356
        10         20         30         40         50         60 
MPTSDKSRQT SAGKSFFGRK LHKERPVEDR WDAHGSWESL APPSSAAGSR SSRYSKRSSI 

        70         80         90        100        110        120 
QSVDFGADID PSLLSTSAGP ITSIPFESLS TDTQSPIPVD YLSKAETSPR KEPSPGHLAK 

       130        140        150        160        170        180 
GVGDFHQYPA WDPSAMRNHQ QFSHPTGPRP PPHAAGVAMS SSATGDKGAR YQQWGRPGSS 

       190        200        210        220        230        240 
AGNAGLSHHS SSTVDSSTNS RMSIDQASIH SSLSSNTRGS SYISTDGSSR TTLPSHSNDR 

       250        260        270        280        290        300 
SNYYAAMNSG RGSSAQGAIP PAQPRVQNTE QYLTRPRDDR VVDQLFLELM QKRGWQNLPE 

       310        320        330        340        350        360 
QARRQMMAYP ASKKWTLVHQ DRLTELQGEQ KRKQKARETH GYDGPSGILE RADEEGSPEW 

       370        380        390        400        410        420 
YVKKVMDDTI TSKQLASLSV SLRTQPISWV KAFVEAQGQI ALTNVLVKIN RKKVTGPVPA 

       430        440        450        460        470        480 
PPSGDKDLDR EYDIVKCLKA LMNNKYGADD ALAHQQIIVA LISSLLSPRL NTRKLVSEVL 

       490        500        510        520        530        540 
TFLCHWAEGQ GHERVLQAMD HVKNHQGETG RFDAWMRIVE VTIDGRGKMG SLVGASEEYR 

       550        560        570        580        590        600 
SGGIGMENLL MEYAVSTMIL INMLVDAPEN DLQLRCHIRA QFISCGIKRL LSKMEGFQYE 

       610        620        630        640        650        660 
VIDKQIEHFR ENEAIDYEDL LQRESSSTKD SIEGEVKDMT DPLQITDAIA SRLNGTRAHD 

       670        680        690        700        710        720 
YFLSALQHLL LIRENSGEDG LRMYQLVDAM LSYVAMDRRL PDLDLRQGLT FTVQSLLDRL 

       730        740        750        760        770        780 
HTDAEARRAY DESLEARQIA EAALAERDEM KAQVELGADG LVRKLQKQIE EQTGIIELQS 

       790        800        810        820        830        840 
RQNEMLKAEL ADVQRLRAQE LQRNELETRE LYLMLRDAQD IAASNAKKSN MGEAETDPAH 

       850        860        870        880        890        900 
MRGILDREKL LTRLEKQLER TKTQFKLEGK VWGQHDPSDR LRELREQMDG DAGPREAFEE 

       910        920        930        940        950        960 
QARLNLSLNP VGSVYRKKTY IQGMEDTATE ELGQTDDEVV YAKARLVDLH RPRMDPEQAT 

       970        980        990       1000       1010       1020 
GLLGEIAAKV PKIDADDAKD EGKPTESEQP AEGAATKGDE QGVDDTVAVD KATAAPPPPP 

      1030       1040       1050       1060       1070       1080 
PPPPAHPGLS GAAPPPPPPP PPPPPGAGAA PPPPPPPPPP PPGGLGGPPP PPPPPPPGGF 

      1090       1100       1110       1120       1130       1140 
GGPPPPPPPP GGFGGPPPPP PPPPGGAFGV PPPPPPPGTV IGGWRANYLA SQGAPSHAIP 

      1150       1160       1170       1180       1190       1200 
VMSSIRPKKK LKALHWDKVD TPQVTVWATH GTTPQEKEEK YVELAKRGVL DEVERLFMAK 

      1210       1220       1230       1240       1250       1260 
ETRIFGGGVA AKQRKDKKQI ISNDLSKNFQ IALSKFSQFP AEEVVRRIIH CDAEILDNMV 

      1270       1280       1290       1300       1310       1320 
VMEFLQRDEM CTVPENVSKL MAPYSKDWTG PDAANTEREQ DPSELTREDQ IYLYTAFELN 

      1330       1340       1350       1360       1370       1380 
HYWKARMRAL ALTRSFEPDY EHISAKLREV VRVSESLRDS VSLMNVLGLI LDIGNFMNDA 

      1390       1400       1410       1420       1430       1440 
NKQAQGFKLS SLARLGMVKD DKNETTFADL VERIVRNQYP EWEDFTEQIS GVIGLQKLNV 

      1450       1460       1470       1480       1490       1500 
DQLRTDAKKY IDNIKNVQAS LDAGNLSDPK KFHPQDRVSQ ITQRSMKDAR RKAEQMQLYL 

      1510       1520       1530       1540       1550       1560 
EEMVKTYDDI MVFYGEDNTD DGARRDFFAK LAAFLQEWKK SKEKNIALEE ARRRTEASLA 

      1570       1580       1590       1600       1610       1620 
RKRINVGLAN GAGAAGDAPV SPATSGAMDS LLEKLRAAAP QAKDQRDRRR RARLKERHQV 

      1630       1640       1650       1660       1670       1680 
RVASGQKIPD LEGAEAPGSG GQNSGATDTN ATDSSLLSPT IQEPEGGSSP IASQSEDVAD 

      1690       1700       1710       1720       1730       1740 
RAASMLQDML RNSPDPERTR RRRESAEEER RKRRLRRRNG ATSGSKDSND TTPLSPVTEP 

      1750       1760       1770       1780       1790 
TSTQGESAEP ENLSLSSPPN GEDPTLNPPT IVLSSDASDT PDDEHRPSTS 

« Hide

References

« Hide 'large scale' references
[1]"The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity."
Harris S.D., Hamer L., Sharpless K.E., Hamer J.E.
EMBO J. 16:3474-3483(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Hamer L., Harris S.D., Sharpless K.E., Hamer J.E.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 143-153; 207; 1071-1109 AND 1644.
[3]"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae."
Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. expand/collapse author list , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.
Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[4]"The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. expand/collapse author list , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[5]"Identification of developmental regulatory genes in Aspergillus nidulans by overexpression."
Marhoul J.F., Adams T.H.
Genetics 139:537-547(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 559-1790, FUNCTION.
Strain: FGSC 26.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U83658 Genomic DNA. Translation: AAB63335.3.
AACD01000109 Genomic DNA. Translation: EAA57863.1. Sequence problems.
L36341 Genomic DNA. Translation: AAA33306.1. Sequence problems.
BN001301 Genomic DNA. Translation: CBF70912.1. Sequence problems.
RefSeqXP_664127.1. XM_659035.1.

3D structure databases

ProteinModelPortalP78621.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANIAT00007295; CADANIAP00007295; CADANIAG00007295.
GeneID2870211.
KEGGani:AN6523.2.

Phylogenomic databases

eggNOGNOG264408.
HOGENOMHOG000200715.
KOK11238.
OrthoDBEOG7G4QPH.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR014767. Diaphanous_autoregulatory.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
[Graphical view]
PfamPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTSM00498. FH2. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 2 hits.
PROSITEPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSEPA_EMENI
AccessionPrimary (citable) accession number: P78621
Secondary accession number(s): C8V0K5, Q00760, Q5AYV7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 14, 2001
Last modified: May 14, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families