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P78621

- SEPA_EMENI

UniProt

P78621 - SEPA_EMENI

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Protein

Cytokinesis protein sepA

Gene

sepA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Involved in cytokinesis. Overexpression results in growth inhibition.1 Publication

GO - Biological processi

  1. actomyosin contractile ring assembly Source: ASPGD
  2. cell septum assembly Source: ASPGD
  3. cellular response to heat Source: ASPGD
  4. establishment or maintenance of cell polarity Source: ASPGD
  5. hyphal growth Source: ASPGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Names & Taxonomyi

Protein namesi
Recommended name:
Cytokinesis protein sepA
Alternative name(s):
Forced expression inhibition of growth A
Protein FH1/2
Gene namesi
Name:sepA
Synonyms:figA
ORF Names:AN6523
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560: Chromosome I

Subcellular locationi

GO - Cellular componenti

  1. hyphal tip Source: ASPGD
  2. spitzenkorper Source: ASPGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17901790Cytokinesis protein sepAPRO_0000194903Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP78621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini274 – 702429GBD/FH3PROSITE-ProRule annotationAdd
BLAST
Domaini955 – 1136182FH1Add
BLAST
Domaini1141 – 1564424FH2PROSITE-ProRule annotationAdd
BLAST
Domaini1581 – 161333DADPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili724 – 81188Sequence AnalysisAdd
BLAST
Coiled coili1435 – 1566132Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1016 – 1117102Pro-richAdd
BLAST
Compositional biasi1608 – 16114Arg/Lys-rich (basic)

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family. BNI1 subfamily.Curated
Contains 1 DAD (diaphanous autoregulatory) domain.PROSITE-ProRule annotation
Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG264408.
HOGENOMiHOG000200715.
InParanoidiP78621.
KOiK11238.
OrthoDBiEOG7G4QPH.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR014767. Diaphanous_autoregulatory.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
[Graphical view]
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78621-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTSDKSRQT SAGKSFFGRK LHKERPVEDR WDAHGSWESL APPSSAAGSR
60 70 80 90 100
SSRYSKRSSI QSVDFGADID PSLLSTSAGP ITSIPFESLS TDTQSPIPVD
110 120 130 140 150
YLSKAETSPR KEPSPGHLAK GVGDFHQYPA WDPSAMRNHQ QFSHPTGPRP
160 170 180 190 200
PPHAAGVAMS SSATGDKGAR YQQWGRPGSS AGNAGLSHHS SSTVDSSTNS
210 220 230 240 250
RMSIDQASIH SSLSSNTRGS SYISTDGSSR TTLPSHSNDR SNYYAAMNSG
260 270 280 290 300
RGSSAQGAIP PAQPRVQNTE QYLTRPRDDR VVDQLFLELM QKRGWQNLPE
310 320 330 340 350
QARRQMMAYP ASKKWTLVHQ DRLTELQGEQ KRKQKARETH GYDGPSGILE
360 370 380 390 400
RADEEGSPEW YVKKVMDDTI TSKQLASLSV SLRTQPISWV KAFVEAQGQI
410 420 430 440 450
ALTNVLVKIN RKKVTGPVPA PPSGDKDLDR EYDIVKCLKA LMNNKYGADD
460 470 480 490 500
ALAHQQIIVA LISSLLSPRL NTRKLVSEVL TFLCHWAEGQ GHERVLQAMD
510 520 530 540 550
HVKNHQGETG RFDAWMRIVE VTIDGRGKMG SLVGASEEYR SGGIGMENLL
560 570 580 590 600
MEYAVSTMIL INMLVDAPEN DLQLRCHIRA QFISCGIKRL LSKMEGFQYE
610 620 630 640 650
VIDKQIEHFR ENEAIDYEDL LQRESSSTKD SIEGEVKDMT DPLQITDAIA
660 670 680 690 700
SRLNGTRAHD YFLSALQHLL LIRENSGEDG LRMYQLVDAM LSYVAMDRRL
710 720 730 740 750
PDLDLRQGLT FTVQSLLDRL HTDAEARRAY DESLEARQIA EAALAERDEM
760 770 780 790 800
KAQVELGADG LVRKLQKQIE EQTGIIELQS RQNEMLKAEL ADVQRLRAQE
810 820 830 840 850
LQRNELETRE LYLMLRDAQD IAASNAKKSN MGEAETDPAH MRGILDREKL
860 870 880 890 900
LTRLEKQLER TKTQFKLEGK VWGQHDPSDR LRELREQMDG DAGPREAFEE
910 920 930 940 950
QARLNLSLNP VGSVYRKKTY IQGMEDTATE ELGQTDDEVV YAKARLVDLH
960 970 980 990 1000
RPRMDPEQAT GLLGEIAAKV PKIDADDAKD EGKPTESEQP AEGAATKGDE
1010 1020 1030 1040 1050
QGVDDTVAVD KATAAPPPPP PPPPAHPGLS GAAPPPPPPP PPPPPGAGAA
1060 1070 1080 1090 1100
PPPPPPPPPP PPGGLGGPPP PPPPPPPGGF GGPPPPPPPP GGFGGPPPPP
1110 1120 1130 1140 1150
PPPPGGAFGV PPPPPPPGTV IGGWRANYLA SQGAPSHAIP VMSSIRPKKK
1160 1170 1180 1190 1200
LKALHWDKVD TPQVTVWATH GTTPQEKEEK YVELAKRGVL DEVERLFMAK
1210 1220 1230 1240 1250
ETRIFGGGVA AKQRKDKKQI ISNDLSKNFQ IALSKFSQFP AEEVVRRIIH
1260 1270 1280 1290 1300
CDAEILDNMV VMEFLQRDEM CTVPENVSKL MAPYSKDWTG PDAANTEREQ
1310 1320 1330 1340 1350
DPSELTREDQ IYLYTAFELN HYWKARMRAL ALTRSFEPDY EHISAKLREV
1360 1370 1380 1390 1400
VRVSESLRDS VSLMNVLGLI LDIGNFMNDA NKQAQGFKLS SLARLGMVKD
1410 1420 1430 1440 1450
DKNETTFADL VERIVRNQYP EWEDFTEQIS GVIGLQKLNV DQLRTDAKKY
1460 1470 1480 1490 1500
IDNIKNVQAS LDAGNLSDPK KFHPQDRVSQ ITQRSMKDAR RKAEQMQLYL
1510 1520 1530 1540 1550
EEMVKTYDDI MVFYGEDNTD DGARRDFFAK LAAFLQEWKK SKEKNIALEE
1560 1570 1580 1590 1600
ARRRTEASLA RKRINVGLAN GAGAAGDAPV SPATSGAMDS LLEKLRAAAP
1610 1620 1630 1640 1650
QAKDQRDRRR RARLKERHQV RVASGQKIPD LEGAEAPGSG GQNSGATDTN
1660 1670 1680 1690 1700
ATDSSLLSPT IQEPEGGSSP IASQSEDVAD RAASMLQDML RNSPDPERTR
1710 1720 1730 1740 1750
RRRESAEEER RKRRLRRRNG ATSGSKDSND TTPLSPVTEP TSTQGESAEP
1760 1770 1780 1790
ENLSLSSPPN GEDPTLNPPT IVLSSDASDT PDDEHRPSTS
Length:1,790
Mass (Da):197,356
Last modified:August 14, 2001 - v2
Checksum:i192136DE2EF2A75B
GO

Sequence cautioni

The sequence CBF70912.1 differs from that shown. Reason: An additional intron is predicted in this sequence to correct a probable sequencing error.
The sequence EAA57863.1 differs from that shown. Reason: An additional intron is predicted in this sequence to correct a probable sequencing error.
The sequence AAA33306.1 differs from that shown. Reason: Frameshift at positions 1071 and 1109.
The sequence AAA33306.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CBF70912.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence EAA57863.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti713 – 7131Missing in EAA57863. (PubMed:16372000)Curated
Sequence conflicti713 – 7131Missing in CBF70912. (PubMed:16372000)Curated
Sequence conflicti1104 – 11041P → T in AAA33306. (PubMed:7713416)Curated
Sequence conflicti1476 – 14761D → V in AAA33306. (PubMed:7713416)Curated
Sequence conflicti1504 – 15041V → L in AAA33306. (PubMed:7713416)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U83658 Genomic DNA. Translation: AAB63335.3.
AACD01000109 Genomic DNA. Translation: EAA57863.1. Sequence problems.
L36341 Genomic DNA. Translation: AAA33306.1. Sequence problems.
BN001301 Genomic DNA. Translation: CBF70912.1. Sequence problems.
RefSeqiXP_664127.1. XM_659035.1.

Genome annotation databases

EnsemblFungiiCADANIAT00007295; CADANIAP00007295; CADANIAG00007295.
GeneIDi2870211.
KEGGiani:AN6523.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U83658 Genomic DNA. Translation: AAB63335.3 .
AACD01000109 Genomic DNA. Translation: EAA57863.1 . Sequence problems.
L36341 Genomic DNA. Translation: AAA33306.1 . Sequence problems.
BN001301 Genomic DNA. Translation: CBF70912.1 . Sequence problems.
RefSeqi XP_664127.1. XM_659035.1.

3D structure databases

ProteinModelPortali P78621.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANIAT00007295 ; CADANIAP00007295 ; CADANIAG00007295 .
GeneIDi 2870211.
KEGGi ani:AN6523.2.

Phylogenomic databases

eggNOGi NOG264408.
HOGENOMi HOG000200715.
InParanoidi P78621.
KOi K11238.
OrthoDBi EOG7G4QPH.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR014767. Diaphanous_autoregulatory.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
[Graphical view ]
Pfami PF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view ]
SMARTi SM00498. FH2. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 2 hits.
PROSITEi PS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity."
    Harris S.D., Hamer L., Sharpless K.E., Hamer J.E.
    EMBO J. 16:3474-3483(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Hamer L., Harris S.D., Sharpless K.E., Hamer J.E.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 143-153; 207; 1071-1109 AND 1644.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  4. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  5. "Identification of developmental regulatory genes in Aspergillus nidulans by overexpression."
    Marhoul J.F., Adams T.H.
    Genetics 139:537-547(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 559-1790, FUNCTION.
    Strain: FGSC 26.

Entry informationi

Entry nameiSEPA_EMENI
AccessioniPrimary (citable) accession number: P78621
Secondary accession number(s): C8V0K5, Q00760, Q5AYV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 14, 2001
Last modified: October 29, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3