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P78621

- SEPA_EMENI

UniProt

P78621 - SEPA_EMENI

Protein

Cytokinesis protein sepA

Gene

sepA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Involved in cytokinesis. Overexpression results in growth inhibition.1 Publication

    GO - Biological processi

    1. actomyosin contractile ring assembly Source: ASPGD
    2. cell septum assembly Source: ASPGD
    3. cellular response to heat Source: ASPGD
    4. establishment or maintenance of cell polarity Source: ASPGD
    5. hyphal growth Source: ASPGD

    Keywords - Biological processi

    Cell cycle, Cell division

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytokinesis protein sepA
    Alternative name(s):
    Forced expression inhibition of growth A
    Protein FH1/2
    Gene namesi
    Name:sepA
    Synonyms:figA
    ORF Names:AN6523
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome I

    Subcellular locationi

    GO - Cellular componenti

    1. hyphal tip Source: ASPGD
    2. spitzenkorper Source: ASPGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 17901790Cytokinesis protein sepAPRO_0000194903Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP78621.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini274 – 702429GBD/FH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini955 – 1136182FH1Add
    BLAST
    Domaini1141 – 1564424FH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1581 – 161333DADPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili724 – 81188Sequence AnalysisAdd
    BLAST
    Coiled coili1435 – 1566132Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1016 – 1117102Pro-richAdd
    BLAST
    Compositional biasi1608 – 16114Arg/Lys-rich (basic)

    Domaini

    The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments By similarity.By similarity

    Sequence similaritiesi

    Belongs to the formin homology family. BNI1 subfamily.Curated
    Contains 1 DAD (diaphanous autoregulatory) domain.PROSITE-ProRule annotation
    Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG264408.
    HOGENOMiHOG000200715.
    KOiK11238.
    OrthoDBiEOG7G4QPH.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR014767. Diaphanous_autoregulatory.
    IPR015425. FH2_Formin.
    IPR010472. FH3_dom.
    IPR010473. GTPase-bd.
    IPR014768. GTPase-bd/formin_homology_3.
    [Graphical view]
    PfamiPF06367. Drf_FH3. 1 hit.
    PF06371. Drf_GBD. 1 hit.
    PF02181. FH2. 1 hit.
    [Graphical view]
    SMARTiSM00498. FH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 2 hits.
    PROSITEiPS51231. DAD. 1 hit.
    PS51444. FH2. 1 hit.
    PS51232. GBD_FH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P78621-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPTSDKSRQT SAGKSFFGRK LHKERPVEDR WDAHGSWESL APPSSAAGSR     50
    SSRYSKRSSI QSVDFGADID PSLLSTSAGP ITSIPFESLS TDTQSPIPVD 100
    YLSKAETSPR KEPSPGHLAK GVGDFHQYPA WDPSAMRNHQ QFSHPTGPRP 150
    PPHAAGVAMS SSATGDKGAR YQQWGRPGSS AGNAGLSHHS SSTVDSSTNS 200
    RMSIDQASIH SSLSSNTRGS SYISTDGSSR TTLPSHSNDR SNYYAAMNSG 250
    RGSSAQGAIP PAQPRVQNTE QYLTRPRDDR VVDQLFLELM QKRGWQNLPE 300
    QARRQMMAYP ASKKWTLVHQ DRLTELQGEQ KRKQKARETH GYDGPSGILE 350
    RADEEGSPEW YVKKVMDDTI TSKQLASLSV SLRTQPISWV KAFVEAQGQI 400
    ALTNVLVKIN RKKVTGPVPA PPSGDKDLDR EYDIVKCLKA LMNNKYGADD 450
    ALAHQQIIVA LISSLLSPRL NTRKLVSEVL TFLCHWAEGQ GHERVLQAMD 500
    HVKNHQGETG RFDAWMRIVE VTIDGRGKMG SLVGASEEYR SGGIGMENLL 550
    MEYAVSTMIL INMLVDAPEN DLQLRCHIRA QFISCGIKRL LSKMEGFQYE 600
    VIDKQIEHFR ENEAIDYEDL LQRESSSTKD SIEGEVKDMT DPLQITDAIA 650
    SRLNGTRAHD YFLSALQHLL LIRENSGEDG LRMYQLVDAM LSYVAMDRRL 700
    PDLDLRQGLT FTVQSLLDRL HTDAEARRAY DESLEARQIA EAALAERDEM 750
    KAQVELGADG LVRKLQKQIE EQTGIIELQS RQNEMLKAEL ADVQRLRAQE 800
    LQRNELETRE LYLMLRDAQD IAASNAKKSN MGEAETDPAH MRGILDREKL 850
    LTRLEKQLER TKTQFKLEGK VWGQHDPSDR LRELREQMDG DAGPREAFEE 900
    QARLNLSLNP VGSVYRKKTY IQGMEDTATE ELGQTDDEVV YAKARLVDLH 950
    RPRMDPEQAT GLLGEIAAKV PKIDADDAKD EGKPTESEQP AEGAATKGDE 1000
    QGVDDTVAVD KATAAPPPPP PPPPAHPGLS GAAPPPPPPP PPPPPGAGAA 1050
    PPPPPPPPPP PPGGLGGPPP PPPPPPPGGF GGPPPPPPPP GGFGGPPPPP 1100
    PPPPGGAFGV PPPPPPPGTV IGGWRANYLA SQGAPSHAIP VMSSIRPKKK 1150
    LKALHWDKVD TPQVTVWATH GTTPQEKEEK YVELAKRGVL DEVERLFMAK 1200
    ETRIFGGGVA AKQRKDKKQI ISNDLSKNFQ IALSKFSQFP AEEVVRRIIH 1250
    CDAEILDNMV VMEFLQRDEM CTVPENVSKL MAPYSKDWTG PDAANTEREQ 1300
    DPSELTREDQ IYLYTAFELN HYWKARMRAL ALTRSFEPDY EHISAKLREV 1350
    VRVSESLRDS VSLMNVLGLI LDIGNFMNDA NKQAQGFKLS SLARLGMVKD 1400
    DKNETTFADL VERIVRNQYP EWEDFTEQIS GVIGLQKLNV DQLRTDAKKY 1450
    IDNIKNVQAS LDAGNLSDPK KFHPQDRVSQ ITQRSMKDAR RKAEQMQLYL 1500
    EEMVKTYDDI MVFYGEDNTD DGARRDFFAK LAAFLQEWKK SKEKNIALEE 1550
    ARRRTEASLA RKRINVGLAN GAGAAGDAPV SPATSGAMDS LLEKLRAAAP 1600
    QAKDQRDRRR RARLKERHQV RVASGQKIPD LEGAEAPGSG GQNSGATDTN 1650
    ATDSSLLSPT IQEPEGGSSP IASQSEDVAD RAASMLQDML RNSPDPERTR 1700
    RRRESAEEER RKRRLRRRNG ATSGSKDSND TTPLSPVTEP TSTQGESAEP 1750
    ENLSLSSPPN GEDPTLNPPT IVLSSDASDT PDDEHRPSTS 1790
    Length:1,790
    Mass (Da):197,356
    Last modified:August 14, 2001 - v2
    Checksum:i192136DE2EF2A75B
    GO

    Sequence cautioni

    The sequence CBF70912.1 differs from that shown. Reason: An additional intron is predicted in this sequence to correct a probable sequencing error.
    The sequence EAA57863.1 differs from that shown. Reason: An additional intron is predicted in this sequence to correct a probable sequencing error.
    The sequence AAA33306.1 differs from that shown. Reason: Frameshift at positions 1071 and 1109.
    The sequence AAA33306.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CBF70912.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence EAA57863.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti713 – 7131Missing in EAA57863. (PubMed:16372000)Curated
    Sequence conflicti713 – 7131Missing in CBF70912. (PubMed:16372000)Curated
    Sequence conflicti1104 – 11041P → T in AAA33306. (PubMed:7713416)Curated
    Sequence conflicti1476 – 14761D → V in AAA33306. (PubMed:7713416)Curated
    Sequence conflicti1504 – 15041V → L in AAA33306. (PubMed:7713416)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U83658 Genomic DNA. Translation: AAB63335.3.
    AACD01000109 Genomic DNA. Translation: EAA57863.1. Sequence problems.
    L36341 Genomic DNA. Translation: AAA33306.1. Sequence problems.
    BN001301 Genomic DNA. Translation: CBF70912.1. Sequence problems.
    RefSeqiXP_664127.1. XM_659035.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00007295; CADANIAP00007295; CADANIAG00007295.
    GeneIDi2870211.
    KEGGiani:AN6523.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U83658 Genomic DNA. Translation: AAB63335.3 .
    AACD01000109 Genomic DNA. Translation: EAA57863.1 . Sequence problems.
    L36341 Genomic DNA. Translation: AAA33306.1 . Sequence problems.
    BN001301 Genomic DNA. Translation: CBF70912.1 . Sequence problems.
    RefSeqi XP_664127.1. XM_659035.1.

    3D structure databases

    ProteinModelPortali P78621.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00007295 ; CADANIAP00007295 ; CADANIAG00007295 .
    GeneIDi 2870211.
    KEGGi ani:AN6523.2.

    Phylogenomic databases

    eggNOGi NOG264408.
    HOGENOMi HOG000200715.
    KOi K11238.
    OrthoDBi EOG7G4QPH.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR014767. Diaphanous_autoregulatory.
    IPR015425. FH2_Formin.
    IPR010472. FH3_dom.
    IPR010473. GTPase-bd.
    IPR014768. GTPase-bd/formin_homology_3.
    [Graphical view ]
    Pfami PF06367. Drf_FH3. 1 hit.
    PF06371. Drf_GBD. 1 hit.
    PF02181. FH2. 1 hit.
    [Graphical view ]
    SMARTi SM00498. FH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 2 hits.
    PROSITEi PS51231. DAD. 1 hit.
    PS51444. FH2. 1 hit.
    PS51232. GBD_FH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity."
      Harris S.D., Hamer L., Sharpless K.E., Hamer J.E.
      EMBO J. 16:3474-3483(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Hamer L., Harris S.D., Sharpless K.E., Hamer J.E.
      Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 143-153; 207; 1071-1109 AND 1644.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    4. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    5. "Identification of developmental regulatory genes in Aspergillus nidulans by overexpression."
      Marhoul J.F., Adams T.H.
      Genetics 139:537-547(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 559-1790, FUNCTION.
      Strain: FGSC 26.

    Entry informationi

    Entry nameiSEPA_EMENI
    AccessioniPrimary (citable) accession number: P78621
    Secondary accession number(s): C8V0K5, Q00760, Q5AYV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3