P78589 (FDFT_CANAX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Squalene synthase
Short name=SQS
Short name=SS
EC=2.5.1.21

Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase

Gene names

Name:

ERG9

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol By similarity.
Catalytic activity	2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.
Cofactor	Magnesium By similarity.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium NADP
Molecular function	Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	farnesyl-diphosphate farnesyltransferase activity Inferred from electronic annotation. Source: EC oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW squalene synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Squalene synthase		PRO_0000067447
Regions
	Transmembrane	295 – 315	21	Helical; Potential
	Transmembrane	428 – 448	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P78589 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 357C11E2918E4863	FASTA	448	51,171
	10 20 30 40 50 60 MGKFLQLLSH PTELKAVIQL FGFRQPLHPG KRDVNDKELG RCYELLNLTS RSFAAVIEEL 70 80 90 100 110 120 HPELRDAVMI FYLVLRALDT IEDDMTIKSS IKIPLLREFD TKLNTKNWTF DGYGPNEKDR 130 140 150 160 170 180 TVLVEFDKIL NVYHRLKPQY QDIIKSITFK MGNGMADYIL DEEFNVYGVA TVEDYNLYCH 190 200 210 220 230 240 YVAGLVGEGL TNLFVLANFG DKTLTENNFA KADSMGLFLQ KTNIIRDYHE DLQDGRSFWP 250 260 270 280 290 300 REIWSKYTEN LQDFHKVKTP AKEFAGVSCI NELVLNALGH VTDCLDYLSL VKDPSSFSFC 310 320 330 340 350 360 AIPQVMAVAT LAEVYNNPKV LHGVVKIRKG TTCRLILESR TLPGVVKIFK EYIQVINHKS 370 380 390 400 410 420 SVRDPNYLKI GIKCGEIEQY CEMIYPNKQA LPPSMKSLPE NKFTKIVASR ESIDLSVQRR 430 440 IEPGNFNCNV VLFGIGALIL SLIYFVLY

References

[1]	"Cloning of Candida albicans squalene synthase gene." Ishii N., Arisawa M., Aoki Y. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10259 / CBS 5796 / DSM 5817 / JCM 2078 / NBRC 1060.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D89610 Genomic DNA. Translation: BAA13995.1.
3D structure databases
ProteinModelPortal	P78589.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG1562.
Enzyme and pathway databases
UniPathway	UPA00767; UER00751.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR006449. Squal_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR01559. squal_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDFT_CANAX

Accession

Primary (citable) accession number: P78589

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program