P78581 (TAN_ASPOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tannase
EC=3.1.1.20

Cleaved into the following 2 chains:

1. Tannase 33 kDa subunit
2. Tannase 30 kDa subunit

Gene names

ORF Names:

AO090103000074

Organism

Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]

Taxonomic identifier

510516 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus oryzae

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes ester bonds of tannic acid to produce gallic acid and glucose.
Catalytic activity	Digallate + H2O = 2 gallate.
Subunit structure	Heterooctamer of 4 33 kDa and 4 30 kDa subunits linked by disulfide bond(s).
Post-translational modification	The protein is glycosylated to a carbohydrate content of 22.7%. The N-terminus of the 30 kDa subunit is blocked.
Sequence similarities	Belongs to the tannase family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Molecular_function	tannase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.1
	Chain	19 – 316	298	Tannase 33 kDa subunit		PRO_0000033587
	Chain	317 – 588	272	Tannase 30 kDa subunit		PRO_0000033588
Sites
	Site	316 – 317	2	Cleavage
Amino acid modifications
	Modified residue	317	1	Pyrrolidone carboxylic acid Probable

Sequences

	Sequence		Length	Mass (Da)
	P78581 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 24DB928DB02BE77D	FASTA	588	64,095
	10 20 30 40 50 60 MRQHSRMAVA ALAAGANAAS FTDVCTVSNV KAALPANGTL LGISMLPSAV TANPLYNQSA 70 80 90 100 110 120 GMGSTTTYDY CNVTVAYTHT GKGDKVVIKY AFPKPSDYEN RFYVAGGGGF SLSSDATGGL 130 140 150 160 170 180 AYGAVGGATD AGYDAFDNSY DEVVLYGNGT INWDATYMFA YQALGEMTRI GKYITKGFYG 190 200 210 220 230 240 QSSDSKVYTY YEGCSDGGRE GMSQVQRWGE EYDGAITGAP AFRFAQQQVH HVFSSEVEQT 250 260 270 280 290 300 LDYYPPPCEL KKIVNATIAA CDPLDGRTDG VVSRTDLCKL NFNLTSIIGE PYYCAAGTST 310 320 330 340 350 360 SLGFGFSNGK RSNVKRQAEG STTSYQPAQN GTVTARGVAV AQAIYDGLHN SKGERAYLSW 370 380 390 400 410 420 QIASELSDAE TEYNSDTGKW ELNIPSTGGE YVTKFIQLLN LDNLSDLNNV TYDTLVDWMN 430 440 450 460 470 480 TGMVRYMDSL QTTLPDLTPF QSSGGKLLHY HGESDPSIPA ASSVHYWQAV RSVMYGDKTE 490 500 510 520 530 540 EEALEALEDW YQFYLIPGAA HCGTNSLQPG PYPENNMEIM IDWVENGNKP SRLNATVSSG 550 560 570 580 TYAGETQMLC QWPKRPLWRG NSSFDCVNDE KSIDSWTYEF PAFKVPVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the gene encoding tannase and a structural study of the tannase subunit from Aspergillus oryzae." Hatamoto O., Watarai T., Kikuchi M., Mizusawa K., Sekine H. Gene 175:215-221(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-43; 159-166; 317-327; 424-426 AND 456-471. Strain: TH.
[2]	"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D63338 Genomic DNA. Translation: BAA09656.1. AP007174 Genomic DNA. Translation: BAE65552.1.
PIR	JC5087.
RefSeq	XP_001826685.1. XM_001826633.1.
3D structure databases
ProteinModelPortal	P78581.
ModBase	Search...
Protein-protein interaction databases
STRING	5062.CADAORAP00009797.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAORAT00010000; CADAORAP00009797; CADAORAG00010000.
GeneID	5998807.
KEGG	aor:AOR_1_142034.
Phylogenomic databases
HOGENOM	HOG000217289.
KO	K10759.
OrthoDB	EOG45MRDK.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16449.
SABIO-RK	P78581.
Family and domain databases
InterPro	IPR011118. Tannase/feruloyl_esterase. [Graphical view]
Pfam	PF07519. Tannase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TAN_ASPOR

Accession

Primary (citable) accession number: P78581
Secondary accession number(s): Q2TYX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program