P78575 (ERG24_ASCIM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta(14)-sterol reductase
EC=1.3.1.70

Alternative name(s):
C-14 sterol reductase
Sterol C14-reductase

Gene names

Name:

ERG3

Organism

Ascobolus immersus

Taxonomic identifier

5191 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Pezizomycetes › Pezizales › Ascobolaceae › Ascobolus

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol By similarity.
Catalytic activity	4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	delta14-sterol reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Delta(14)-sterol reductase		PRO_0000207495
Regions
	Transmembrane	12 – 32	21	Helical; Potential
	Transmembrane	67 – 87	21	Helical; Potential
	Transmembrane	109 – 129	21	Helical; Potential
	Transmembrane	230 – 250	21	Helical; Potential
	Transmembrane	267 – 287	21	Helical; Potential
	Transmembrane	290 – 310	21	Helical; Potential
	Transmembrane	349 – 369	21	Helical; Potential
	Transmembrane	376 – 396	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P78575 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 7A61F01A4701B59D	FASTA	430	49,126
	10 20 30 40 50 60 MGGKDYEFGG PIGTGVLMLI LPPISHYLHF LITPRGAPPP EFWSAPLETL KSVTPTFSSL 70 80 90 100 110 120 FSLHATLAVA AYYLLLVALM YVLPAEIAEG VVLKDGSRLK YRCNAFTTFL VFFTFLGTMT 130 140 150 160 170 180 VLEGPTWWFW SYLTDNFAQL QSASIVFSYA MSLWVYIRSY RPMPKGKEVI LSPVGFKGNH 190 200 210 220 230 240 IHDFWMGREL NPRIGEWLDI KQLHELRPGL MGWILFNLAW TVKQYNTHGF VSDSIVLVNL 250 260 270 280 290 300 FETWYVVDAL WNESKVLTTM DITTDGLGVM LLFGNAVWVP FMYCLQARYL ASFPVHLGLL 310 320 330 340 350 360 GIAGVLAVQF TGYAIFRGAN NQKNAFRTNP ADPAVSHLKF MTTKSGSKLL ISGWWGVARH 370 380 390 400 410 420 VNYFGDWIMA WSYCLTTGFN TPLTYFYVIY FGILLLHRDR RDEAKCREKY GKDWDRYCKV 430 VKWRIIPGIY

References

[1]	Kasbekar D.P. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RN42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y10624 Genomic DNA. Translation: CAA71650.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG24_ASCIM

Accession

Primary (citable) accession number: P78575

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1997
Last modified:	June 28, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program