P78568 (PUT2_AGABI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta-1-pyrroline-5-carboxylate dehydrogenase
Short name=P5C dehydrogenase
EC=1.5.1.12

Gene names

Name:

pruA

Organism

Agaricus bisporus (Common mushroom)

Taxonomic identifier

5341 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Agaricaceae › Agaricus

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Proline metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 546	546	Delta-1-pyrroline-5-carboxylate dehydrogenase		PRO_0000056501
Regions
	Nucleotide binding	279 – 284	6	NAD By similarity
Sites
	Active site	297	1	Proton acceptor By similarity
	Active site	331	1	Nucleophile By similarity
	Site	195	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P78568 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 6B795E09AC242945	FASTA	546	59,757
	10 20 30 40 50 60 MATAQLATFK IPPVSNEPML SYAPGSPERA GLQAALAEMQ SQLPFEVPCI INGQEVRTNN 70 80 90 100 110 120 IQKQPMPHDH ARHLCTFHEG SPELVEKATC GALQAKDGWE TMPWNDRAAI FLKAADLASG 130 140 150 160 170 180 KYRYKLMAAT MLGQGKNTWQ AEIDAAAELA DFFRFGVSYV EELYAQQPPK NAPGCWNRTE 190 200 210 220 230 240 YRPLEGFVLA VSPFNFTAIG GNLPGSPALV GNVVVWKPAP AATYSNYLVF KILSEAGVPP 250 260 270 280 290 300 GVIQFIPGGA EIVQAAIQSP NFRSLHFTGS TNVFKSLWKD ISSNLDKYKV YPRIVGETGG 310 320 330 340 350 360 KNWHVIHKSA EVRNAVLQSV RGAFEYQGQK CSALSRLYVS RSVWENGFKT QYLEEIAKIK 370 380 390 400 410 420 VGPCLDWNNY MGPVIGRRAY DNITGFIKKA KEEGGEVLIG GSGDDSKGFF IQPTVILTKV 430 440 450 460 470 480 PRSTTMVGEI FGPVVTAYVF EDSDYEKTLE LIDTTSIYGL TGAIFASERQ ALLTATNRSR 490 500 510 520 530 540 NAAGNIYYNE KCTGAVVGQQ PFGGARGSGT NDKAGSISIF YRFVSARSIK ENFVGLEDFH YPSNLV

References

[1]	"The Agaricus bisporus pruA gene encodes a cytosolic delta 1-pyrroline-5-carboxylate dehydrogenase which is expressed in fruit bodies but not in gill tissue." Schaap P.J., Mueller Y., Sonnenberg A.S.M., van Griensven L.J.L.D., Visser J. Appl. Environ. Microbiol. 63:57-62(1997) [PubMed: 8979339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Horst H39.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95584 Genomic DNA. Translation: CAA64836.1.
3D structure databases
ProteinModelPortal	P78568.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR005931. Delta1-pyrroline-5-COlate_DH-1. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR01236. D1pyr5carbox1. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUT2_AGABI

Accession

Primary (citable) accession number: P78568

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	October 19, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program