P78560 (CRADD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Death domain-containing protein CRADD

Alternative name(s):
Caspase and RIP adapter with death domain
RIP-associated protein with a death domain

Gene names

Name:	CRADD
Synonyms:	RAIDD

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.
Subunit structure	Interacts with LRDD. Ref.4 Ref.5
Tissue specificity	Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
Domain	Contains a death domain involved in the binding of RIP protein. The CARD domain mediates the interaction with caspase-2.
Sequence similarities	Contains 1 CARD domain. Contains 1 death domain.

Ontologies

Keywords
Biological process	Apoptosis
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cellular response to mechanical stimulus Inferred from expression pattern. Source: UniProtKB induction of apoptosis via death domain receptors Traceable author statement. Source: ProtInc signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	death domain binding Inferred from physical interaction Ref.1. Source: BHF-UCL protease binding Inferred from physical interaction Ref.1. Source: BHF-UCL protein binding, bridging Inferred from physical interaction Ref.1. Source: BHF-UCL
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CASP2	P42575	9	EBI-520375,EBI-520342
PIDD	Q9HB75	6	EBI-520375,EBI-520427

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 199

199

Death domain-containing protein CRADD

PRO_0000079326

Regions

Domain

1 – 91

91

CARD

Domain

116 – 188

73

Death

Secondary structure

1

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199

Helix Strand Turn

Details...

Helix

5 – 18

14

Helix

27 – 33

7

Helix

38 – 44

7

Beta strand

48 – 50

3

Helix

51 – 60

10

Turn

61 – 63

3

Helix

69 – 75

7

Helix

79 – 92

14

Helix

110 – 112

3

Helix

117 – 126

10

Helix

131 – 137

7

Helix

142 – 151

10

Helix

156 – 171

16

Helix

172 – 174

3

Helix

177 – 186

10

Helix

192 – 197

6

Sequences

	Sequence		Length	Mass (Da)
	P78560 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 3437CC612C85E402	FASTA	199	22,745
	10 20 30 40 50 60 MEARDKQVLR SLRLELGAEV LVEGLVLQYL YQEGILTENH IQEINAQTTG LRKTMLLLDI 70 80 90 100 110 120 LPSRGPKAFD TFLDSLQEFP WVREKLKKAR EEAMTDLPAG DRLTGIPSHI LNSSPSDRQI 130 140 150 160 170 180 NQLAQRLGPE WEPMVLSLGL SQTDIYRCKA NHPHNVQSQV VEAFIRWRQR FGKQATFQSL 190 HNGLRAVEVD PSLLLHMLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CRADD, a novel human apoptotic adaptor molecule for caspase-2, and FasL/tumor necrosis factor receptor-interacting protein RIP." Ahmad M., Srinivasula S.M., Wang L., Talanian R.V., Litwack G., Fernandes-Alnemri T., Alnemri E.S. Cancer Res. 57:615-619(1997) [PubMed: 9044836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"RAIDD is a new 'death' adaptor molecule." Duan H., Dixit V.M. Nature 385:86-89(1997) [PubMed: 8985253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[4]	"The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress." Tinel A., Tschopp J. Science 304:843-846(2004) [PubMed: 15073321] [Abstract] Cited for: INTERACTION WITH LRDD.
[5]	"Functional connection between p53 and caspase-2 is essential for apoptosis induced by DNA damage." Vakifahmetoglu H., Olsson M., Orrenius S., Zhivotovsky B. Oncogene 25:5683-5692(2006) [PubMed: 16652156] [Abstract] Cited for: INTERACTION WITH LRDD.
[6]	"Solution structure of the RAIDD CARD and model for CARD/CARD interaction in caspase-2 and caspase-9 recruitment." Chou J.J., Matsuo H., Duan H., Wagner G. Cell 94:171-180(1998) [PubMed: 9695946] [Abstract] Cited for: STRUCTURE BY NMR OF 1-100.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U84388 mRNA. Translation: AAC50952.1.
U79115 mRNA. Translation: AAB42217.1.
BC017042 mRNA. Translation: AAH17042.1.

IPI

IPI00020364.

RefSeq

NP_003796.1. NM_003805.3.

UniGene

Hs.38533.
Hs.591016.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O71	X-ray	2.00	A	95-199	[»]
2OF5	X-ray	3.20	A/B/C/D/E/F/G	94-199	[»]
3CRD	NMR	-	A	1-100	[»]

ProteinModelPortal

P78560.

SMR

P78560. Positions 1-100, 109-199.

ModBase

Search...

Protein-protein interaction databases

IntAct

P78560. 11 interactions.

MINT

MINT-1520976.

STRING

P78560.

Polymorphism databases

DMDM

2498833.

Proteomic databases

PRIDE

P78560.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000332896; ENSP00000327647; ENSG00000169372.

GeneID

8738.

KEGG

hsa:8738.

UCSC

uc001tda.1. human.

Organism-specific databases

CTD

8738.

GeneCards

GC12P094004.

H-InvDB

HIX0010886.

HGNC

HGNC:2340. CRADD.

HPA

CAB005337.

MIM

603454. gene.

neXtProt

NX_P78560.

PharmGKB

PA26860.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG05544.

HOGENOM

HBG281400.

HOVERGEN

HBG051112.

InParanoid

P78560.

OMA

GKQATFQ.

OrthoDB

EOG4JT06K.

Enzyme and pathway databases

Pathway_Interaction_DB

caspase_pathway. Caspase cascade in apoptosis.
ceramidepathway. Ceramide signaling pathway.
hivnefpathway. HIV-1 Nef: Negative effector of Fas and TNF-alpha.

Gene expression databases

ArrayExpress

P78560.

Bgee

P78560.

CleanEx

HS_CRADD.

Genevestigator

P78560.

Family and domain databases

InterPro

IPR001315. CARD.
IPR000488. Death.
IPR011029. DEATH-like.
[Graphical view]

Gene3D

G3DSA:1.10.533.10. DEATH_like. 2 hits.

KO

K02832.

Pfam

PF00619. CARD. 1 hit.
PF00531. Death. 1 hit.
[Graphical view]

SMART

SM00114. CARD. 1 hit.
SM00005. DEATH. 1 hit.
[Graphical view]

SUPFAM

SSF47986. DEATH_like. 2 hits.

PROSITE

PS50209. CARD. 1 hit.
PS50017. DEATH_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

32779.

SOURCE

Search...

Entry information

Entry name

CRADD_HUMAN

Accession

Primary (citable) accession number: P78560

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.