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Protein

Death domain-containing protein CRADD

Gene

CRADD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.

GO - Molecular functioni

  • death domain binding Source: BHF-UCL
  • protease binding Source: BHF-UCL
  • protein binding, bridging Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169372-MONOMER.
ReactomeiR-HSA-6803207. TP53 Regulates Transcription of Caspase Activators and Caspases.
SignaLinkiP78560.

Names & Taxonomyi

Protein namesi
Recommended name:
Death domain-containing protein CRADD
Alternative name(s):
Caspase and RIP adapter with death domain
RIP-associated protein with a death domain
Gene namesi
Name:CRADD
Synonyms:RAIDD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:2340. CRADD.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Mental retardation, autosomal recessive 34 (MRT34)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRT34 is a non-syndromic form. Affected individuals have mildly delayed development and significantly impaired cognitive function, precluding independent living and self-care. Speech is rudimentary, but articulate; autism is not present.
See also OMIM:614499
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_067536128G → R in MRT34. 1 PublicationCorresponds to variant rs387906861dbSNPEnsembl.1

Keywords - Diseasei

Disease mutation, Mental retardation

Organism-specific databases

DisGeNETi8738.
MalaCardsiCRADD.
MIMi614499. phenotype.
OpenTargetsiENSG00000169372.
Orphaneti88616. Autosomal recessive non-syndromic intellectual disability.
PharmGKBiPA26860.

Polymorphism and mutation databases

BioMutaiCRADD.
DMDMi2498833.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000793261 – 199Death domain-containing protein CRADDAdd BLAST199

Proteomic databases

EPDiP78560.
PaxDbiP78560.
PeptideAtlasiP78560.
PRIDEiP78560.

PTM databases

iPTMnetiP78560.
PhosphoSitePlusiP78560.

Expressioni

Tissue specificityi

Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.

Gene expression databases

BgeeiENSG00000169372.
CleanExiHS_CRADD.
ExpressionAtlasiP78560. baseline and differential.
GenevisibleiP78560. HS.

Organism-specific databases

HPAiCAB005337.
HPA046546.

Interactioni

Subunit structurei

Interacts with PIDD.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APPL2Q8NEU83EBI-520375,EBI-741261
BBS5Q8N3I73EBI-520375,EBI-2892592
CASP2P4257516EBI-520375,EBI-520342
KCTD9Q7L2733EBI-520375,EBI-4397613
PIDD1Q9HB758EBI-520375,EBI-520427
taxP140793EBI-520375,EBI-9675698From a different organism.
TRIM54Q9BYV23EBI-520375,EBI-2130429

GO - Molecular functioni

  • death domain binding Source: BHF-UCL
  • protease binding Source: BHF-UCL
  • protein binding, bridging Source: BHF-UCL

Protein-protein interaction databases

BioGridi114275. 21 interactors.
IntActiP78560. 17 interactors.
MINTiMINT-1520976.
STRINGi9606.ENSP00000327647.

Structurei

Secondary structure

1199
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 18Combined sources14
Helixi27 – 33Combined sources7
Helixi38 – 44Combined sources7
Beta strandi48 – 50Combined sources3
Helixi51 – 60Combined sources10
Turni61 – 63Combined sources3
Helixi69 – 75Combined sources7
Helixi79 – 92Combined sources14
Helixi110 – 112Combined sources3
Helixi117 – 126Combined sources10
Helixi131 – 137Combined sources7
Helixi142 – 151Combined sources10
Helixi156 – 171Combined sources16
Helixi172 – 174Combined sources3
Helixi177 – 186Combined sources10
Helixi192 – 197Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O71X-ray2.00A94-199[»]
2OF5X-ray3.20A/B/C/D/E/F/G94-199[»]
3CRDNMR-A1-100[»]
ProteinModelPortaliP78560.
SMRiP78560.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78560.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 91CARDPROSITE-ProRule annotationAdd BLAST91
Domaini116 – 188DeathPROSITE-ProRule annotationAdd BLAST73

Domaini

Contains a death domain involved in the binding of RIP protein.
The CARD domain mediates the interaction with caspase-2.

Sequence similaritiesi

Contains 1 CARD domain.PROSITE-ProRule annotation
Contains 1 death domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IJAM. Eukaryota.
ENOG4111SYE. LUCA.
GeneTreeiENSGT00390000014448.
HOGENOMiHOG000111965.
HOVERGENiHBG051112.
InParanoidiP78560.
KOiK02832.
OMAiLGPEWEH.
OrthoDBiEOG091G0M3P.
PhylomeDBiP78560.
TreeFamiTF333055.

Family and domain databases

Gene3Di1.10.533.10. 2 hits.
InterProiIPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR000488. Death_domain.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF00531. Death. 1 hit.
[Graphical view]
SMARTiSM00114. CARD. 1 hit.
SM00005. DEATH. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 2 hits.
PROSITEiPS50209. CARD. 1 hit.
PS50017. DEATH_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P78560-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEARDKQVLR SLRLELGAEV LVEGLVLQYL YQEGILTENH IQEINAQTTG
60 70 80 90 100
LRKTMLLLDI LPSRGPKAFD TFLDSLQEFP WVREKLKKAR EEAMTDLPAG
110 120 130 140 150
DRLTGIPSHI LNSSPSDRQI NQLAQRLGPE WEPMVLSLGL SQTDIYRCKA
160 170 180 190
NHPHNVQSQV VEAFIRWRQR FGKQATFQSL HNGLRAVEVD PSLLLHMLE
Length:199
Mass (Da):22,745
Last modified:May 1, 1997 - v1
Checksum:i3437CC612C85E402
GO
Isoform 2 (identifier: P78560-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-199: DRLTGIPSHI...DPSLLLHMLE → CWP

Note: No experimental confirmation available.
Show »
Length:103
Mass (Da):11,826
Checksum:i04DFF4A1F09D0B56
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_067536128G → R in MRT34. 1 PublicationCorresponds to variant rs387906861dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056892101 – 199DRLTG…LHMLE → CWP in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84388 mRNA. Translation: AAC50952.1.
U79115 mRNA. Translation: AAB42217.1.
AK294986 mRNA. Translation: BAH11941.1.
AC012085 Genomic DNA. No translation available.
AC012464 Genomic DNA. No translation available.
AC025261 Genomic DNA. No translation available.
BC017042 mRNA. Translation: AAH17042.1.
CCDSiCCDS9048.1. [P78560-1]
RefSeqiNP_001307028.1. NM_001320099.1. [P78560-1]
NP_001307029.1. NM_001320100.1.
NP_001307030.1. NM_001320101.1.
NP_003796.1. NM_003805.4. [P78560-1]
XP_016875633.1. XM_017020144.1. [P78560-2]
UniGeneiHs.38533.
Hs.591016.
Hs.660031.
Hs.719191.

Genome annotation databases

EnsembliENST00000332896; ENSP00000327647; ENSG00000169372. [P78560-1]
ENST00000542893; ENSP00000439068; ENSG00000169372. [P78560-1]
ENST00000551065; ENSP00000448425; ENSG00000169372. [P78560-2]
GeneIDi8738.
KEGGihsa:8738.
UCSCiuc058rts.1. human. [P78560-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84388 mRNA. Translation: AAC50952.1.
U79115 mRNA. Translation: AAB42217.1.
AK294986 mRNA. Translation: BAH11941.1.
AC012085 Genomic DNA. No translation available.
AC012464 Genomic DNA. No translation available.
AC025261 Genomic DNA. No translation available.
BC017042 mRNA. Translation: AAH17042.1.
CCDSiCCDS9048.1. [P78560-1]
RefSeqiNP_001307028.1. NM_001320099.1. [P78560-1]
NP_001307029.1. NM_001320100.1.
NP_001307030.1. NM_001320101.1.
NP_003796.1. NM_003805.4. [P78560-1]
XP_016875633.1. XM_017020144.1. [P78560-2]
UniGeneiHs.38533.
Hs.591016.
Hs.660031.
Hs.719191.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O71X-ray2.00A94-199[»]
2OF5X-ray3.20A/B/C/D/E/F/G94-199[»]
3CRDNMR-A1-100[»]
ProteinModelPortaliP78560.
SMRiP78560.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114275. 21 interactors.
IntActiP78560. 17 interactors.
MINTiMINT-1520976.
STRINGi9606.ENSP00000327647.

PTM databases

iPTMnetiP78560.
PhosphoSitePlusiP78560.

Polymorphism and mutation databases

BioMutaiCRADD.
DMDMi2498833.

Proteomic databases

EPDiP78560.
PaxDbiP78560.
PeptideAtlasiP78560.
PRIDEiP78560.

Protocols and materials databases

DNASUi8738.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000332896; ENSP00000327647; ENSG00000169372. [P78560-1]
ENST00000542893; ENSP00000439068; ENSG00000169372. [P78560-1]
ENST00000551065; ENSP00000448425; ENSG00000169372. [P78560-2]
GeneIDi8738.
KEGGihsa:8738.
UCSCiuc058rts.1. human. [P78560-1]

Organism-specific databases

CTDi8738.
DisGeNETi8738.
GeneCardsiCRADD.
HGNCiHGNC:2340. CRADD.
HPAiCAB005337.
HPA046546.
MalaCardsiCRADD.
MIMi603454. gene.
614499. phenotype.
neXtProtiNX_P78560.
OpenTargetsiENSG00000169372.
Orphaneti88616. Autosomal recessive non-syndromic intellectual disability.
PharmGKBiPA26860.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJAM. Eukaryota.
ENOG4111SYE. LUCA.
GeneTreeiENSGT00390000014448.
HOGENOMiHOG000111965.
HOVERGENiHBG051112.
InParanoidiP78560.
KOiK02832.
OMAiLGPEWEH.
OrthoDBiEOG091G0M3P.
PhylomeDBiP78560.
TreeFamiTF333055.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169372-MONOMER.
ReactomeiR-HSA-6803207. TP53 Regulates Transcription of Caspase Activators and Caspases.
SignaLinkiP78560.

Miscellaneous databases

ChiTaRSiCRADD. human.
EvolutionaryTraceiP78560.
GeneWikiiCRADD.
GenomeRNAii8738.
PROiP78560.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169372.
CleanExiHS_CRADD.
ExpressionAtlasiP78560. baseline and differential.
GenevisibleiP78560. HS.

Family and domain databases

Gene3Di1.10.533.10. 2 hits.
InterProiIPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR000488. Death_domain.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF00531. Death. 1 hit.
[Graphical view]
SMARTiSM00114. CARD. 1 hit.
SM00005. DEATH. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 2 hits.
PROSITEiPS50209. CARD. 1 hit.
PS50017. DEATH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRADD_HUMAN
AccessioniPrimary (citable) accession number: P78560
Secondary accession number(s): B7Z2Q5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.