Death domain-containing protein CRADD

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Reviewed, UniProtKB/Swiss-Prot P78560 (CRADD_HUMAN)

Last modified July 7, 2009. Version 83. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Death domain-containing protein CRADD
Alternative name(s):
    Caspase and RIP adapter with death domain
    RIP-associated protein with a death domain

Gene names

Name:	CRADD
Synonyms:	RAIDD

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruites caspase-2 to the TNFR-1 signalling complex.
Subunit structure	Interacts with LRDD. Ref.4 Ref.5
Tissue specificity	Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
Domain	Contains a death domain involved in the binding of RIP protein. The CARD domain mediates the interaction with caspase-2.
Sequence similarities	Contains 1 CARD domain. Contains 1 death domain.

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Ontologies

Keywords
Biological process	Apoptosis
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis via death domain receptors Ref.1 Traceable author statement. Source: ProtInc proteolysis Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro death domain binding Ref.1 Inferred from physical interaction. Source: UniProtKB protease binding Ref.1 Inferred from physical interaction. Source: UniProtKB protein binding, bridging Ref.1 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
CASP2	P42575	3	EBI-520375,EBI-520342
LRDD	Q9HB75	1	EBI-520375,EBI-520427

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 199

199

Death domain-containing protein CRADD

PRO_0000079326

Regions

Domain

1 – 91

CARD

Domain

116 – 188

Death

Secondary structure

199

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P78560-1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 3437CC612C85E402
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FASTA

199

22,745

        10         20         30         40         50         60 
MEARDKQVLR SLRLELGAEV LVEGLVLQYL YQEGILTENH IQEINAQTTG LRKTMLLLDI 

        70         80         90        100        110        120 
LPSRGPKAFD TFLDSLQEFP WVREKLKKAR EEAMTDLPAG DRLTGIPSHI LNSSPSDRQI 

       130        140        150        160        170        180 
NQLAQRLGPE WEPMVLSLGL SQTDIYRCKA NHPHNVQSQV VEAFIRWRQR FGKQATFQSL 

       190 
HNGLRAVEVD PSLLLHMLE

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CRADD, a novel human apoptotic adaptor molecule for caspase-2, and FasL/tumor necrosis factor receptor-interacting protein RIP." Ahmad M., Srinivasula S.M., Wang L., Talanian R.V., Litwack G., Fernandes-Alnemri T., Alnemri E.S. Cancer Res. 57:615-619(1997) [PubMed: 9044836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"RAIDD is a new 'death' adaptor molecule." Duan H., Dixit V.M. Nature 385:86-89(1997) [PubMed: 8985253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[4]	"The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress." Tinel A., Tschopp J. Science 304:843-846(2004) [PubMed: 15073321] [Abstract] Cited for: INTERACTION WITH LRDD.
[5]	"Functional connection between p53 and caspase-2 is essential for apoptosis induced by DNA damage." Vakifahmetoglu H., Olsson M., Orrenius S., Zhivotovsky B. Oncogene 25:5683-5692(2006) [PubMed: 16652156] [Abstract] Cited for: INTERACTION WITH LRDD.
[6]	"Solution structure of the RAIDD CARD and model for CARD/CARD interaction in caspase-2 and caspase-9 recruitment." Chou J.J., Matsuo H., Duan H., Wagner G. Cell 94:171-180(1998) [PubMed: 9695946] [Abstract] Cited for: STRUCTURE BY NMR OF 1-100.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U84388 mRNA. Translation: AAC50952.1.
U79115 mRNA. Translation: AAB42217.1.
BC017042 mRNA. Translation: AAH17042.1.

IPI

IPI00020364.

RefSeq

NP_003796.1.

UniGene

Hs.38533
Hs.591016
Hs.715196

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O71	X-ray	2.00	A	95-199	[»]
2OF5	X-ray	3.20	A/B/C/D/E/F/G	94-199	[»]
3CRD	NMR	-	A	1-100	[»]

SMR

P78560. Positions 1-100.

ModBase

Search...

Protein-protein interaction databases

IntAct

P78560. 6 interactions.

Proteomic databases

PRIDE

P78560.

Genome annotation databases

Ensembl

ENSG00000169372. Homo sapiens. [Contig view]

GeneID

8738.

KEGG

hsa:8738.

UCSC

uc001tda.1. human.

Organism-specific databases

GeneCards

GC12P092573.

H-InvDB

HIX0010886.

HGNC

HGNC:2340. CRADD.

HPA

CAB005337.

MIM

603454. gene.

PharmGKB

PA26860.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P78560.

HOVERGEN

P78560.

OMA

P78560. ILTENHV.

Enzyme and pathway databases

Pathway_Interaction_DB

caspase_pathway. Caspase cascade in apoptosis.
ceramidepathway. Ceramide signaling pathway.
hivnefpathway. HIV-1 Nef: Negative effector of Fas and TNF-alpha.

Gene expression databases

ArrayExpress

P78560.

Bgee

P78560.

CleanEx

HS_CRADD.

Family and domain databases

InterPro

IPR001315. CARD.
IPR000488. Death.
IPR011029. DEATH-like.
IPR002398. Pept_C14_p45.
[Graphical view]

Gene3D

G3DSA:1.10.533.10. DEATH_like. 2 hits.

PANTHER

PTHR10454. Pept_C14_p45. 1 hit.

Pfam

PF00619. CARD. 1 hit.
PF00531. Death. 1 hit.
[Graphical view]

SMART

SM00114. CARD. 1 hit.
SM00005. DEATH. 1 hit.
[Graphical view]

PROSITE

PS50209. CARD. 1 hit.
PS50017. DEATH_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

32779.

SOURCE

Search...

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Entry information

Entry name

CRADD_HUMAN

Accession

Primary (citable) accession number: P78560

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	July 7, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents