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P78556 (CCL20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C motif chemokine 20
Alternative name(s):
Beta-chemokine exodus-1
CC chemokine LARC
Liver and activation-regulated chemokine
Macrophage inflammatory protein 3 alpha
Short name=MIP-3-alpha
Small-inducible cytokine A20

Cleaved into the following 3 chains:

  1. CCL20(1-67)
  2. CCL20(1-64)
  3. CCL20(2-70)
Gene names
Name:CCL20
Synonyms:LARC, MIP3A, SCYA20
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length96 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Ref.8

Subunit structure

Binds to and activate CCR6.

Subcellular location

Secreted.

Tissue specificity

Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.

Induction

By bacterial lipopolysaccharides (LPS), TNF and IFNG/IFN-gamma. Induced by phorbol myristate acetate (PMA) in U-937 cell line and bowes melanoma. Repressed by IL10/interleukin-10.

Post-translational modification

C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RALBP1Q153112EBI-3913209,EBI-749285

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P78556-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P78556-2)

The sequence of this isoform differs from the canonical sequence as follows:
     26-26: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Ref.2
Chain27 – 9670C-C motif chemokine 20
PRO_0000005217
Chain27 – 9367CCL20(1-67)
PRO_0000041854
Chain27 – 9064CCL20(1-64)
PRO_0000041855
Chain28 – 9669CCL20(2-70)
PRO_0000041856

Amino acid modifications

Disulfide bond32 ↔ 58
Disulfide bond33 ↔ 74

Natural variations

Alternative sequence261Missing in isoform 2.
VSP_001061
Natural variant471V → M.
Corresponds to variant rs1049617 [ dbSNP | Ensembl ].
VAR_011915

Secondary structure

............. 96
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 1ED19EC2E1AC70AD

FASTA9610,762
        10         20         30         40         50         60 
MCCTKSLLLA ALMSVLLLHL CGESEAASNF DCCLGYTDRI LHPKFIVGFT RQLANEGCDI 

        70         80         90 
NAIIFHTKKK LSVCANPKQT WVKYIVRLLS KKVKNM 

« Hide

Isoform 2 [UniParc].

Checksum: BFDB243C51B59960
Show »

FASTA9510,691

References

« Hide 'large scale' references
[1]"Identification through bioinformatics of two new macrophage proinflammatory human chemokines: MIP-3alpha and MIP-3beta."
Rossi D.L., Vicari A.P., Franz-Bacon K., McClanahan T.K., Zlotnik A.
J. Immunol. 158:1033-1036(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Molecular cloning of a novel human CC chemokine liver and activation-regulated chemokine (LARC) expressed in liver. Chemotactic activity for lymphocytes and gene localization on chromosome 2."
Hieshima K., Imai T., Opdenakker G., van Damme J., Kusuda J., Tei H., Sakaki Y., Takatsuki K., Miura R., Yoshie O., Nomiyama H.
J. Biol. Chem. 272:5846-5853(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF N-TERMINUS.
Tissue: Liver.
[3]"Cloning and characterization of Exodus, a novel beta-chemokine."
Hromas R.A., Gray P.W., Chantry D., Godiska R., Krathwohl M., Fife K., Bell G.I., Takeda J., Aronica S., Gordon M., Cooper S., Broxmeyer H.E., Klemsz M.J.
Blood 89:3315-3322(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Pancreas.
[4]"Genomic organization of the CC chemokine mip-3alpha/CCL20/larc/ exodus/SCYA20, showing gene structure, splice variants, and chromosome localization."
Nelson R.T., Boyd J., Gladue R.P., Paradis T., Thomas R., Cunningham A.C., Lira P., Brissette W.H., Hayes L., Hames L.M., Neote K.S., McColl S.R.
Genomics 73:28-37(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Liver.
[5]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Liver.
[7]"Regulated production and molecular diversity of human liver and activation-regulated chemokine/macrophage inflammatory protein-3 alpha from normal and transformed cells."
Schutyser E., Struyf S., Menten P., Lenaerts J.-P., Conings R., Put W., Wuyts A., Proost P., Van Damme J.
J. Immunol. 165:4470-4477(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF CCL20(1-67); CCL20(1-64) AND CCL20(2-70), PROTEOLYTIC PROCESSING OF C-TERMINAL.
[8]"The structure of human macrophage inflammatory protein-3alpha /CCL20. Linking antimicrobial and CC chemokine receptor-6-binding activities with human beta-defensins."
Hoover D.M., Boulegue C., Yang D., Oppenheim J.J., Tucker K., Lu W., Lubkowski J.
J. Biol. Chem. 277:37647-37654(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 27-96, FUNCTION AS AN ANTIMICROBIAL PROTEIN.
+Additional computationally mapped references.

Web resources

Wikipedia

CCL20 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U77035 mRNA. Translation: AAC50943.1.
D86955 mRNA. Translation: BAA13191.1.
U64197 mRNA. Translation: AAB61534.1.
AC073065 Genomic DNA. Translation: AAX93214.1.
BC020698 mRNA. Translation: AAH20698.1.
RefSeqNP_001123518.1. NM_001130046.1.
NP_004582.1. NM_004591.2.
UniGeneHs.75498.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1M8AX-ray1.70A/B27-96[»]
2HCIX-ray1.81A/B27-95[»]
2JYONMR-A27-96[»]
ProteinModelPortalP78556.
SMRP78556. Positions 31-91.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112267. 3 interactions.
DIPDIP-5864N.
IntActP78556. 3 interactions.
MINTMINT-1525084.
STRING9606.ENSP00000351671.

Polymorphism databases

DMDM2829668.

Proteomic databases

PaxDbP78556.
PRIDEP78556.

Protocols and materials databases

DNASU6364.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000358813; ENSP00000351671; ENSG00000115009. [P78556-1]
ENST00000409189; ENSP00000386273; ENSG00000115009. [P78556-2]
GeneID6364.
KEGGhsa:6364.
UCSCuc002vpl.2. human. [P78556-1]
uc002vpm.2. human. [P78556-2]

Organism-specific databases

CTD6364.
GeneCardsGC02P228678.
HGNCHGNC:10619. CCL20.
MIM601960. gene.
neXtProtNX_P78556.
PharmGKBPA35551.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG44269.
HOGENOMHOG000036685.
HOVERGENHBG017871.
InParanoidP78556.
KOK14625.
OMAACDINAV.
OrthoDBEOG7B31QP.
PhylomeDBP78556.
TreeFamTF334888.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeP78556.
CleanExHS_CCL20.
GenevestigatorP78556.

Family and domain databases

InterProIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
PROSITEPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP78556.
GeneWikiCCL20.
GenomeRNAi6364.
NextBio24728.
PMAP-CutDBP78556.
PROP78556.
SOURCESearch...

Entry information

Entry nameCCL20_HUMAN
AccessionPrimary (citable) accession number: P78556
Secondary accession number(s): Q53S51, Q99664
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM