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P78556

- CCL20_HUMAN

UniProt

P78556 - CCL20_HUMAN

Protein

C-C motif chemokine 20

Gene

CCL20

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.1 Publication

    GO - Molecular functioni

    1. chemokine activity Source: ProtInc
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cell-cell signaling Source: ProtInc
    2. cell chemotaxis Source: GOC
    3. chemokinesis Source: Ensembl
    4. chemotaxis Source: ProtInc
    5. defense response to bacterium Source: UniProtKB-KW
    6. immune response Source: ProtInc
    7. inflammatory response Source: ProtInc
    8. positive regulation of T cell migration Source: MGI
    9. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Cytokine

    Keywords - Biological processi

    Chemotaxis, Inflammatory response

    Enzyme and pathway databases

    ReactomeiREACT_15344. Chemokine receptors bind chemokines.
    REACT_19231. G alpha (i) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-C motif chemokine 20
    Alternative name(s):
    Beta-chemokine exodus-1
    CC chemokine LARC
    Liver and activation-regulated chemokine
    Macrophage inflammatory protein 3 alpha
    Short name:
    MIP-3-alpha
    Small-inducible cytokine A20
    Cleaved into the following 3 chains:
    Gene namesi
    Name:CCL20
    Synonyms:LARC, MIP3A, SCYA20
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:10619. CCL20.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. extracellular space Source: ProtInc

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35551.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 26261 PublicationAdd
    BLAST
    Chaini27 – 9670C-C motif chemokine 20PRO_0000005217Add
    BLAST
    Chaini27 – 9367CCL20(1-67)PRO_0000041854Add
    BLAST
    Chaini27 – 9064CCL20(1-64)PRO_0000041855Add
    BLAST
    Chaini28 – 9669CCL20(2-70)PRO_0000041856Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi32 ↔ 58
    Disulfide bondi33 ↔ 74

    Post-translational modificationi

    C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.1 Publication

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP78556.
    PRIDEiP78556.

    Miscellaneous databases

    PMAP-CutDBP78556.

    Expressioni

    Tissue specificityi

    Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.

    Inductioni

    By bacterial lipopolysaccharides (LPS), TNF and IFNG/IFN-gamma. Induced by phorbol myristate acetate (PMA) in U-937 cell line and bowes melanoma. Repressed by IL10/interleukin-10.

    Gene expression databases

    BgeeiP78556.
    CleanExiHS_CCL20.
    GenevestigatoriP78556.

    Interactioni

    Subunit structurei

    Binds to and activate CCR6.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RALBP1Q153112EBI-3913209,EBI-749285

    Protein-protein interaction databases

    BioGridi112267. 3 interactions.
    DIPiDIP-5864N.
    IntActiP78556. 3 interactions.
    MINTiMINT-1525084.
    STRINGi9606.ENSP00000351671.

    Structurei

    Secondary structure

    1
    96
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi43 – 453
    Beta strandi46 – 527
    Turni54 – 574
    Beta strandi58 – 603
    Beta strandi62 – 676
    Beta strandi72 – 754
    Helixi80 – 8910

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1M8AX-ray1.70A/B27-96[»]
    2HCIX-ray1.81A/B27-95[»]
    2JYONMR-A27-96[»]
    ProteinModelPortaliP78556.
    SMRiP78556. Positions 31-91.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP78556.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG44269.
    HOGENOMiHOG000036685.
    HOVERGENiHBG017871.
    InParanoidiP78556.
    KOiK14625.
    OMAiACDINAV.
    OrthoDBiEOG7B31QP.
    PhylomeDBiP78556.
    TreeFamiTF334888.

    Family and domain databases

    InterProiIPR000827. Chemokine_CC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    SMARTiSM00199. SCY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.
    PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P78556-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCCTKSLLLA ALMSVLLLHL CGESEAASNF DCCLGYTDRI LHPKFIVGFT   50
    RQLANEGCDI NAIIFHTKKK LSVCANPKQT WVKYIVRLLS KKVKNM 96
    Length:96
    Mass (Da):10,762
    Last modified:May 1, 1997 - v1
    Checksum:i1ED19EC2E1AC70AD
    GO
    Isoform 2 (identifier: P78556-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         26-26: Missing.

    Show »
    Length:95
    Mass (Da):10,691
    Checksum:iBFDB243C51B59960
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti47 – 471V → M.
    Corresponds to variant rs1049617 [ dbSNP | Ensembl ].
    VAR_011915

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei26 – 261Missing in isoform 2. 3 PublicationsVSP_001061

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77035 mRNA. Translation: AAC50943.1.
    D86955 mRNA. Translation: BAA13191.1.
    U64197 mRNA. Translation: AAB61534.1.
    AC073065 Genomic DNA. Translation: AAX93214.1.
    BC020698 mRNA. Translation: AAH20698.1.
    CCDSiCCDS2469.1. [P78556-1]
    CCDS46536.1. [P78556-2]
    RefSeqiNP_001123518.1. NM_001130046.1. [P78556-2]
    NP_004582.1. NM_004591.2. [P78556-1]
    UniGeneiHs.75498.

    Genome annotation databases

    EnsembliENST00000358813; ENSP00000351671; ENSG00000115009. [P78556-1]
    ENST00000409189; ENSP00000386273; ENSG00000115009. [P78556-2]
    GeneIDi6364.
    KEGGihsa:6364.
    UCSCiuc002vpl.2. human. [P78556-1]
    uc002vpm.2. human. [P78556-2]

    Polymorphism databases

    DMDMi2829668.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Wikipedia

    CCL20 entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77035 mRNA. Translation: AAC50943.1 .
    D86955 mRNA. Translation: BAA13191.1 .
    U64197 mRNA. Translation: AAB61534.1 .
    AC073065 Genomic DNA. Translation: AAX93214.1 .
    BC020698 mRNA. Translation: AAH20698.1 .
    CCDSi CCDS2469.1. [P78556-1 ]
    CCDS46536.1. [P78556-2 ]
    RefSeqi NP_001123518.1. NM_001130046.1. [P78556-2 ]
    NP_004582.1. NM_004591.2. [P78556-1 ]
    UniGenei Hs.75498.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1M8A X-ray 1.70 A/B 27-96 [» ]
    2HCI X-ray 1.81 A/B 27-95 [» ]
    2JYO NMR - A 27-96 [» ]
    ProteinModelPortali P78556.
    SMRi P78556. Positions 31-91.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112267. 3 interactions.
    DIPi DIP-5864N.
    IntActi P78556. 3 interactions.
    MINTi MINT-1525084.
    STRINGi 9606.ENSP00000351671.

    Polymorphism databases

    DMDMi 2829668.

    Proteomic databases

    PaxDbi P78556.
    PRIDEi P78556.

    Protocols and materials databases

    DNASUi 6364.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000358813 ; ENSP00000351671 ; ENSG00000115009 . [P78556-1 ]
    ENST00000409189 ; ENSP00000386273 ; ENSG00000115009 . [P78556-2 ]
    GeneIDi 6364.
    KEGGi hsa:6364.
    UCSCi uc002vpl.2. human. [P78556-1 ]
    uc002vpm.2. human. [P78556-2 ]

    Organism-specific databases

    CTDi 6364.
    GeneCardsi GC02P228678.
    HGNCi HGNC:10619. CCL20.
    MIMi 601960. gene.
    neXtProti NX_P78556.
    PharmGKBi PA35551.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG44269.
    HOGENOMi HOG000036685.
    HOVERGENi HBG017871.
    InParanoidi P78556.
    KOi K14625.
    OMAi ACDINAV.
    OrthoDBi EOG7B31QP.
    PhylomeDBi P78556.
    TreeFami TF334888.

    Enzyme and pathway databases

    Reactomei REACT_15344. Chemokine receptors bind chemokines.
    REACT_19231. G alpha (i) signalling events.

    Miscellaneous databases

    EvolutionaryTracei P78556.
    GeneWikii CCL20.
    GenomeRNAii 6364.
    NextBioi 24728.
    PMAP-CutDB P78556.
    PROi P78556.
    SOURCEi Search...

    Gene expression databases

    Bgeei P78556.
    CleanExi HS_CCL20.
    Genevestigatori P78556.

    Family and domain databases

    InterProi IPR000827. Chemokine_CC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    SMARTi SM00199. SCY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    PROSITEi PS00472. SMALL_CYTOKINES_CC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification through bioinformatics of two new macrophage proinflammatory human chemokines: MIP-3alpha and MIP-3beta."
      Rossi D.L., Vicari A.P., Franz-Bacon K., McClanahan T.K., Zlotnik A.
      J. Immunol. 158:1033-1036(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Molecular cloning of a novel human CC chemokine liver and activation-regulated chemokine (LARC) expressed in liver. Chemotactic activity for lymphocytes and gene localization on chromosome 2."
      Hieshima K., Imai T., Opdenakker G., van Damme J., Kusuda J., Tei H., Sakaki Y., Takatsuki K., Miura R., Yoshie O., Nomiyama H.
      J. Biol. Chem. 272:5846-5853(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF N-TERMINUS.
      Tissue: Liver.
    3. "Cloning and characterization of Exodus, a novel beta-chemokine."
      Hromas R.A., Gray P.W., Chantry D., Godiska R., Krathwohl M., Fife K., Bell G.I., Takeda J., Aronica S., Gordon M., Cooper S., Broxmeyer H.E., Klemsz M.J.
      Blood 89:3315-3322(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Pancreas.
    4. "Genomic organization of the CC chemokine mip-3alpha/CCL20/larc/ exodus/SCYA20, showing gene structure, splice variants, and chromosome localization."
      Nelson R.T., Boyd J., Gladue R.P., Paradis T., Thomas R., Cunningham A.C., Lira P., Brissette W.H., Hayes L., Hames L.M., Neote K.S., McColl S.R.
      Genomics 73:28-37(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: Liver.
    5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Liver.
    7. "Regulated production and molecular diversity of human liver and activation-regulated chemokine/macrophage inflammatory protein-3 alpha from normal and transformed cells."
      Schutyser E., Struyf S., Menten P., Lenaerts J.-P., Conings R., Put W., Wuyts A., Proost P., Van Damme J.
      J. Immunol. 165:4470-4477(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF CCL20(1-67); CCL20(1-64) AND CCL20(2-70), PROTEOLYTIC PROCESSING OF C-TERMINAL.
    8. "The structure of human macrophage inflammatory protein-3alpha /CCL20. Linking antimicrobial and CC chemokine receptor-6-binding activities with human beta-defensins."
      Hoover D.M., Boulegue C., Yang D., Oppenheim J.J., Tucker K., Lu W., Lubkowski J.
      J. Biol. Chem. 277:37647-37654(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 27-96, FUNCTION AS AN ANTIMICROBIAL PROTEIN.

    Entry informationi

    Entry nameiCCL20_HUMAN
    AccessioniPrimary (citable) accession number: P78556
    Secondary accession number(s): Q53S51, Q99664
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 142 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3