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Protein

ETS-related transcription factor Elf-3

Gene

ELF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity).By similarity21 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi273 – 355ETSPROSITE-ProRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

  • blastocyst development Source: Ensembl
  • epidermis development Source: UniProtKB
  • epithelial cell differentiation Source: UniProtKB
  • extracellular matrix organization Source: Ensembl
  • inflammatory response Source: UniProtKB
  • mammary gland involution Source: UniProtKB
  • multicellular organism development Source: ProtInc
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of Notch signaling pathway Source: Reactome
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription by RNA polymerase II Source: GO_Central
  • transcription by RNA polymerase II Source: ProtInc

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding, Repressor
Biological processDifferentiation, Inflammatory response, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1912408 Pre-NOTCH Transcription and Translation
SIGNORiP78545

Names & Taxonomyi

Protein namesi
Recommended name:
ETS-related transcription factor Elf-3
Alternative name(s):
E74-like factor 3
Epithelial-restricted with serine box
Epithelium-restricted Ets protein ESX
Epithelium-specific Ets transcription factor 1
Short name:
ESE-1
Gene namesi
Name:ELF3Imported
Synonyms:ERTImported, ESXImported, JEN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000163435.15
HGNCiHGNC:3318 ELF3
MIMi602191 gene
neXtProtiNX_P78545

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi247 – 250RGRP → AAAA: No effect on transcriptional repression on KRT4 promoter. 1 Publication4
Mutagenesisi315W → A: Partially abrogates repressive effect on the KRT4 promoter; when associated with A-319. 1 Publication1
Mutagenesisi319K → A: Partially abrogates repressive effect on the KRT4 promoter; when associated with A-315. 1 Publication1
Mutagenesisi334 – 337RYYY → AAAA: Partially abrogates repressive effect on the KRT4 promoter. 1 Publication4

Organism-specific databases

DisGeNETi1999
OpenTargetsiENSG00000163435
PharmGKBiPA27746

Polymorphism and mutation databases

BioMutaiELF3
DMDMi74739735

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002876811 – 371ETS-related transcription factor Elf-3Add BLAST371

Proteomic databases

MaxQBiP78545
PaxDbiP78545
PeptideAtlasiP78545
PRIDEiP78545

PTM databases

iPTMnetiP78545
PhosphoSitePlusiP78545

Expressioni

Tissue specificityi

Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin.4 Publications

Inductioni

Transcriptionally regulated by ERBB2 receptor signaling in breast cancer epithelial cells. Up-regulated by phorbol 12-myristate 13-acetate (PMA) in bronchial epithelial cells. By retinoic acid in MCF-7 mammary epithelial cells (at protein level).3 Publications

Gene expression databases

BgeeiENSG00000163435
CleanExiHS_ELF3
ExpressionAtlasiP78545 baseline and differential
GenevisibleiP78545 HS

Organism-specific databases

HPAiHPA003316
HPA003479

Interactioni

Subunit structurei

Interacts with TBP. Interacts with CREBBP and EP300; these act as transcriptional coactivators of ELF3 and positively modulate its function. Interacts with XRCC5/KU86 and XRCC6/KU70; these inhibit the ability of ELF3 to bind DNA and negatively modulate its transcriptional activity. Associated with CLND7 and POU2F3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRKCIP417432EBI-1057285,EBI-286199

Protein-protein interaction databases

BioGridi108314, 30 interactors
IntActiP78545, 32 interactors
STRINGi9606.ENSP00000352673

Structurei

Secondary structure

1371
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi60 – 62Combined sources3
Helixi64 – 66Combined sources3
Helixi69 – 82Combined sources14
Turni92 – 94Combined sources3
Helixi100 – 104Combined sources5
Helixi107 – 113Combined sources7
Helixi115 – 117Combined sources3
Helixi118 – 130Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E8PNMR-A48-132[»]
ProteinModelPortaliP78545
SMRiP78545
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78545

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 132PNTPROSITE-ProRule annotationAdd BLAST87

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi137 – 1459aaTAD9

Domaini

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Sequence similaritiesi

Belongs to the ETS family.Sequence analysis

Phylogenomic databases

eggNOGiKOG3804 Eukaryota
ENOG4111K4J LUCA
GeneTreeiENSGT00760000118996
HOVERGENiHBG074143
InParanoidiP78545
KOiK09429
OMAiRDGFPDY
OrthoDBiEOG091G0BE6
PhylomeDBiP78545
TreeFamiTF318679

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR033074 Elf3
IPR000418 Ets_dom
IPR003118 Pointed_dom
IPR013761 SAM/pointed_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR11849:SF13 PTHR11849:SF13, 1 hit
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PF02198 SAM_PNT, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SM00251 SAM_PNT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS50061 ETS_DOMAIN_3, 1 hit
PS51433 PNT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms may exist.1 Publication
Isoform 11 Publication (identifier: P78545-1) [UniParc]FASTAAdd to basket
Also known as: ESE-1b1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATCEISNI FSNYFSAMYS SEDSTLASVP PAATFGADDL VLTLSNPQMS
60 70 80 90 100
LEGTEKASWL GEQPQFWSKT QVLDWISYQV EKNKYDASAI DFSRCDMDGA
110 120 130 140 150
TLCNCALEEL RLVFGPLGDQ LHAQLRDLTS SSSDELSWII ELLEKDGMAF
160 170 180 190 200
QEALDPGPFD QGSPFAQELL DDGQQASPYH PGSCGAGAPS PGSSDVSTAG
210 220 230 240 250
TGASRSSHSS DSGGSDVDLD PTDGKLFPSD GFRDCKKGDP KHGKRKRGRP
260 270 280 290 300
RKLSKEYWDC LEGKKSKHAP RGTHLWEFIR DILIHPELNE GLMKWENRHE
310 320 330 340 350
GVFKFLRSEA VAQLWGQKKK NSNMTYEKLS RAMRYYYKRE ILERVDGRRL
360 370
VYKFGKNSSG WKEEEVLQSR N
Length:371
Mass (Da):41,454
Last modified:May 1, 1997 - v1
Checksum:i81A23A7DECBE2EF9
GO
Isoform 21 Publication (identifier: P78545-2) [UniParc]FASTAAdd to basket
Also known as: ESE-1a1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     173-195: Missing.

Show »
Length:348
Mass (Da):39,357
Checksum:iAE2A6D3633CA305E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3A → T in AAM70481 (PubMed:14997048).Curated1
Sequence conflicti55 – 56EK → GE in CAD29859 (PubMed:12119107).Curated2
Sequence conflicti68S → L in AAM70481 (PubMed:14997048).Curated1
Sequence conflicti129 – 130TS → SA in CAD29859 (PubMed:12119107).Curated2
Sequence conflicti134D → Y in AAM70481 (PubMed:14997048).Curated1
Sequence conflicti160 – 161DQ → GE in CAD29859 (PubMed:12119107).Curated2
Sequence conflicti201T → A in CAD29859 (PubMed:12119107).Curated1
Sequence conflicti242H → L in CAG33387 (Ref. 9) Curated1
Sequence conflicti244K → E in CAD97611 (PubMed:17974005).Curated1
Sequence conflicti257Y → C in AAM70481 (PubMed:14997048).Curated1
Sequence conflicti269A → G in CAD29859 (PubMed:12119107).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048945317Q → K. Corresponds to variant dbSNP:rs1135542Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052433173 – 195Missing in isoform 2. 1 PublicationAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73843 mRNA Translation: AAB65823.1
U73844 mRNA Translation: AAB65824.1
U97156 mRNA Translation: AAC51884.1
U66894 mRNA Translation: AAB58075.1
AF016295 mRNA Translation: AAB96586.1
AF017307 mRNA Translation: AAB67238.1
AF110184 Genomic DNA Translation: AAD45237.1
BN000001 Genomic DNA Translation: CAD29859.1
AF517841 mRNA Translation: AAM70481.1
CR457106 mRNA Translation: CAG33387.1
AK312273 mRNA Translation: BAG35203.1
BX537368 mRNA Translation: CAD97611.1
AL691482 Genomic DNA Translation: CAI14961.1
CH471067 Genomic DNA Translation: EAW91389.1
BC003569 mRNA Translation: AAH03569.1
CCDSiCCDS1419.1 [P78545-1]
RefSeqiNP_001107781.1, NM_001114309.1 [P78545-1]
NP_004424.3, NM_004433.4 [P78545-1]
UniGeneiHs.603657

Genome annotation databases

EnsembliENST00000359651; ENSP00000352673; ENSG00000163435 [P78545-1]
ENST00000367283; ENSP00000356252; ENSG00000163435 [P78545-1]
ENST00000367284; ENSP00000356253; ENSG00000163435 [P78545-1]
GeneIDi1999
KEGGihsa:1999
UCSCiuc001gxg.5 human [P78545-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiELF3_HUMAN
AccessioniPrimary (citable) accession number: P78545
Secondary accession number(s): B2R5Q6
, Q6IAP8, Q7RU03, Q7Z3X2, Q8NFG2, Q99718
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 1997
Last modified: March 28, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health