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Protein

ETS-related transcription factor Elf-3

Gene

ELF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity).By similarity21 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi273 – 355ETSPROSITE-ProRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

  • blastocyst development Source: Ensembl
  • epidermis development Source: UniProtKB
  • epithelial cell differentiation Source: UniProtKB
  • extracellular matrix organization Source: Ensembl
  • inflammatory response Source: UniProtKB
  • mammary gland involution Source: UniProtKB
  • multicellular organism development Source: ProtInc
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: GO_Central
  • transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Inflammatory response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163435-MONOMER.
SIGNORiP78545.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS-related transcription factor Elf-3
Alternative name(s):
E74-like factor 3
Epithelial-restricted with serine box
Epithelium-restricted Ets protein ESX
Epithelium-specific Ets transcription factor 1
Short name:
ESE-1
Gene namesi
Name:ELF3Imported
Synonyms:ERTImported, ESXImported, JEN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:3318. ELF3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi247 – 250RGRP → AAAA: No effect on transcriptional repression on KRT4 promoter. 1 Publication4
Mutagenesisi315W → A: Partially abrogates repressive effect on the KRT4 promoter; when associated with A-319. 1 Publication1
Mutagenesisi319K → A: Partially abrogates repressive effect on the KRT4 promoter; when associated with A-315. 1 Publication1
Mutagenesisi334 – 337RYYY → AAAA: Partially abrogates repressive effect on the KRT4 promoter. 1 Publication4

Organism-specific databases

DisGeNETi1999.
OpenTargetsiENSG00000163435.
PharmGKBiPA27746.

Polymorphism and mutation databases

BioMutaiELF3.
DMDMi74739735.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002876811 – 371ETS-related transcription factor Elf-3Add BLAST371

Proteomic databases

MaxQBiP78545.
PaxDbiP78545.
PeptideAtlasiP78545.
PRIDEiP78545.

PTM databases

iPTMnetiP78545.
PhosphoSitePlusiP78545.

Expressioni

Tissue specificityi

Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin.4 Publications

Inductioni

Transcriptionally regulated by ERBB2 receptor signaling in breast cancer epithelial cells. Up-regulated by phorbol 12-myristate 13-acetate (PMA) in bronchial epithelial cells. By retinoic acid in MCF-7 mammary epithelial cells (at protein level).3 Publications

Gene expression databases

BgeeiENSG00000163435.
CleanExiHS_ELF3.
ExpressionAtlasiP78545. baseline and differential.
GenevisibleiP78545. HS.

Organism-specific databases

HPAiHPA003316.
HPA003479.

Interactioni

Subunit structurei

Interacts with TBP. Interacts with CREBBP and EP300; these act as transcriptional coactivators of ELF3 and positively modulate its function. Interacts with XRCC5/KU86 and XRCC6/KU70; these inhibit the ability of ELF3 to bind DNA and negatively modulate its transcriptional activity. Associated with CLND7 and POU2F3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRKCIP417432EBI-1057285,EBI-286199

Protein-protein interaction databases

BioGridi108314. 30 interactors.
IntActiP78545. 4 interactors.
STRINGi9606.ENSP00000352673.

Structurei

Secondary structure

1371
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi60 – 62Combined sources3
Helixi64 – 66Combined sources3
Helixi69 – 82Combined sources14
Turni92 – 94Combined sources3
Helixi100 – 104Combined sources5
Helixi107 – 113Combined sources7
Helixi115 – 117Combined sources3
Helixi118 – 130Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E8PNMR-A48-132[»]
ProteinModelPortaliP78545.
SMRiP78545.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78545.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 132PNTPROSITE-ProRule annotationAdd BLAST87

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi137 – 1459aaTAD9

Domaini

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Sequence similaritiesi

Belongs to the ETS family.Sequence analysis
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3804. Eukaryota.
ENOG4111K4J. LUCA.
GeneTreeiENSGT00760000118996.
HOVERGENiHBG074143.
InParanoidiP78545.
KOiK09429.
OMAiRDGFPDY.
OrthoDBiEOG091G0BE6.
PhylomeDBiP78545.
TreeFamiTF318679.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR033074. Elf3.
IPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11849:SF13. PTHR11849:SF13. 2 hits.
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms may exist.1 Publication
Isoform 11 Publication (identifier: P78545-1) [UniParc]FASTAAdd to basket
Also known as: ESE-1b1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATCEISNI FSNYFSAMYS SEDSTLASVP PAATFGADDL VLTLSNPQMS
60 70 80 90 100
LEGTEKASWL GEQPQFWSKT QVLDWISYQV EKNKYDASAI DFSRCDMDGA
110 120 130 140 150
TLCNCALEEL RLVFGPLGDQ LHAQLRDLTS SSSDELSWII ELLEKDGMAF
160 170 180 190 200
QEALDPGPFD QGSPFAQELL DDGQQASPYH PGSCGAGAPS PGSSDVSTAG
210 220 230 240 250
TGASRSSHSS DSGGSDVDLD PTDGKLFPSD GFRDCKKGDP KHGKRKRGRP
260 270 280 290 300
RKLSKEYWDC LEGKKSKHAP RGTHLWEFIR DILIHPELNE GLMKWENRHE
310 320 330 340 350
GVFKFLRSEA VAQLWGQKKK NSNMTYEKLS RAMRYYYKRE ILERVDGRRL
360 370
VYKFGKNSSG WKEEEVLQSR N
Length:371
Mass (Da):41,454
Last modified:May 1, 1997 - v1
Checksum:i81A23A7DECBE2EF9
GO
Isoform 21 Publication (identifier: P78545-2) [UniParc]FASTAAdd to basket
Also known as: ESE-1a1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     173-195: Missing.

Show »
Length:348
Mass (Da):39,357
Checksum:iAE2A6D3633CA305E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3A → T in AAM70481 (PubMed:14997048).Curated1
Sequence conflicti55 – 56EK → GE in CAD29859 (PubMed:12119107).Curated2
Sequence conflicti68S → L in AAM70481 (PubMed:14997048).Curated1
Sequence conflicti129 – 130TS → SA in CAD29859 (PubMed:12119107).Curated2
Sequence conflicti134D → Y in AAM70481 (PubMed:14997048).Curated1
Sequence conflicti160 – 161DQ → GE in CAD29859 (PubMed:12119107).Curated2
Sequence conflicti201T → A in CAD29859 (PubMed:12119107).Curated1
Sequence conflicti242H → L in CAG33387 (Ref. 9) Curated1
Sequence conflicti244K → E in CAD97611 (PubMed:17974005).Curated1
Sequence conflicti257Y → C in AAM70481 (PubMed:14997048).Curated1
Sequence conflicti269A → G in CAD29859 (PubMed:12119107).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048945317Q → K.Corresponds to variant rs1135542dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052433173 – 195Missing in isoform 2. 1 PublicationAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73843 mRNA. Translation: AAB65823.1.
U73844 mRNA. Translation: AAB65824.1.
U97156 mRNA. Translation: AAC51884.1.
U66894 mRNA. Translation: AAB58075.1.
AF016295 mRNA. Translation: AAB96586.1.
AF017307 mRNA. Translation: AAB67238.1.
AF110184 Genomic DNA. Translation: AAD45237.1.
BN000001 Genomic DNA. Translation: CAD29859.1.
AF517841 mRNA. Translation: AAM70481.1.
CR457106 mRNA. Translation: CAG33387.1.
AK312273 mRNA. Translation: BAG35203.1.
BX537368 mRNA. Translation: CAD97611.1.
AL691482 Genomic DNA. Translation: CAI14961.1.
CH471067 Genomic DNA. Translation: EAW91389.1.
BC003569 mRNA. Translation: AAH03569.1.
CCDSiCCDS1419.1. [P78545-1]
RefSeqiNP_001107781.1. NM_001114309.1. [P78545-1]
NP_004424.3. NM_004433.4. [P78545-1]
UniGeneiHs.603657.

Genome annotation databases

EnsembliENST00000359651; ENSP00000352673; ENSG00000163435. [P78545-1]
ENST00000367283; ENSP00000356252; ENSG00000163435. [P78545-1]
ENST00000367284; ENSP00000356253; ENSG00000163435. [P78545-1]
GeneIDi1999.
KEGGihsa:1999.
UCSCiuc001gxg.5. human. [P78545-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73843 mRNA. Translation: AAB65823.1.
U73844 mRNA. Translation: AAB65824.1.
U97156 mRNA. Translation: AAC51884.1.
U66894 mRNA. Translation: AAB58075.1.
AF016295 mRNA. Translation: AAB96586.1.
AF017307 mRNA. Translation: AAB67238.1.
AF110184 Genomic DNA. Translation: AAD45237.1.
BN000001 Genomic DNA. Translation: CAD29859.1.
AF517841 mRNA. Translation: AAM70481.1.
CR457106 mRNA. Translation: CAG33387.1.
AK312273 mRNA. Translation: BAG35203.1.
BX537368 mRNA. Translation: CAD97611.1.
AL691482 Genomic DNA. Translation: CAI14961.1.
CH471067 Genomic DNA. Translation: EAW91389.1.
BC003569 mRNA. Translation: AAH03569.1.
CCDSiCCDS1419.1. [P78545-1]
RefSeqiNP_001107781.1. NM_001114309.1. [P78545-1]
NP_004424.3. NM_004433.4. [P78545-1]
UniGeneiHs.603657.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E8PNMR-A48-132[»]
ProteinModelPortaliP78545.
SMRiP78545.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108314. 30 interactors.
IntActiP78545. 4 interactors.
STRINGi9606.ENSP00000352673.

PTM databases

iPTMnetiP78545.
PhosphoSitePlusiP78545.

Polymorphism and mutation databases

BioMutaiELF3.
DMDMi74739735.

Proteomic databases

MaxQBiP78545.
PaxDbiP78545.
PeptideAtlasiP78545.
PRIDEiP78545.

Protocols and materials databases

DNASUi1999.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359651; ENSP00000352673; ENSG00000163435. [P78545-1]
ENST00000367283; ENSP00000356252; ENSG00000163435. [P78545-1]
ENST00000367284; ENSP00000356253; ENSG00000163435. [P78545-1]
GeneIDi1999.
KEGGihsa:1999.
UCSCiuc001gxg.5. human. [P78545-1]

Organism-specific databases

CTDi1999.
DisGeNETi1999.
GeneCardsiELF3.
HGNCiHGNC:3318. ELF3.
HPAiHPA003316.
HPA003479.
MIMi602191. gene.
neXtProtiNX_P78545.
OpenTargetsiENSG00000163435.
PharmGKBiPA27746.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3804. Eukaryota.
ENOG4111K4J. LUCA.
GeneTreeiENSGT00760000118996.
HOVERGENiHBG074143.
InParanoidiP78545.
KOiK09429.
OMAiRDGFPDY.
OrthoDBiEOG091G0BE6.
PhylomeDBiP78545.
TreeFamiTF318679.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163435-MONOMER.
SIGNORiP78545.

Miscellaneous databases

ChiTaRSiELF3. human.
EvolutionaryTraceiP78545.
GenomeRNAii1999.
PROiP78545.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163435.
CleanExiHS_ELF3.
ExpressionAtlasiP78545. baseline and differential.
GenevisibleiP78545. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR033074. Elf3.
IPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11849:SF13. PTHR11849:SF13. 2 hits.
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiELF3_HUMAN
AccessioniPrimary (citable) accession number: P78545
Secondary accession number(s): B2R5Q6
, Q6IAP8, Q7RU03, Q7Z3X2, Q8NFG2, Q99718
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.