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P78543

- BTG2_HUMAN

UniProt

P78543 - BTG2_HUMAN

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Protein

Protein BTG2

Gene
BTG2, PC3
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Activates mRNA deadenyltion in a CNOT6 and CNOT7-dependent manner. In vitro can inhibit deadenylase activity of CNOT7 and CNOT8. Involved in cell cycle regulation. Could be involved in the growth arrest and differentiation of the neuronal precursors By similarity. Modulates transcription regulation mediated by ESR1. Involved in mitochondrial depolarization and neurite outgrowth.5 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl

GO - Biological processi

  1. anterior/posterior pattern specification Source: Ensembl
  2. associative learning Source: Ensembl
  3. cellular response to DNA damage stimulus Source: MGI
  4. central nervous system neuron development Source: Ensembl
  5. dentate gyrus development Source: Ensembl
  6. DNA repair Source: ProtInc
  7. negative regulation of cell proliferation Source: UniProtKB
  8. negative regulation of neural precursor cell proliferation Source: Ensembl
  9. negative regulation of neuron apoptotic process Source: Ensembl
  10. negative regulation of translation Source: UniProtKB
  11. neuron projection development Source: UniProtKB
  12. positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: MGI
  13. protein methylation Source: Ensembl
  14. response to electrical stimulus Source: Ensembl
  15. response to mechanical stimulus Source: Ensembl
  16. response to organic cyclic compound Source: Ensembl
  17. response to peptide hormone Source: Ensembl
  18. skeletal muscle cell differentiation Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein BTG2
Alternative name(s):
BTG family member 2
NGF-inducible anti-proliferative protein PC3
Gene namesi
Name:BTG2
Synonyms:PC3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:1131. BTG2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi53 – 531H → A: Impairs interaction with CNOT7 and CNOT8. 1 Publication
Mutagenesisi65 – 651Y → A: Abolishes interaction with CNOT7 and CNOT8. 2 Publications
Mutagenesisi75 – 751D → A: Abolishes interaction with CNOT7 and CNOT8. 1 Publication
Mutagenesisi103 – 1031W → A: Abolishes interaction with CNOT7 and CNOT8; impairs anti-proliferative activity. 2 Publications
Mutagenesisi105 – 1051D → A: Impairs interaction with CNOT7 and CNOT8. 1 Publication
Mutagenesisi115 – 1151E → A: Impairs interaction with CNOT7. Inhibits CNOT7 mRNA deadenylase activity. 1 Publication
Mutagenesisi147 – 1471S → A: Impairs phosphorylation by MAPK1 and MAPK3, and decreases PIN1-binding. 1 Publication
Mutagenesisi148 – 1481P → A: Impairs PIN1-binding. 1 Publication
Mutagenesisi149 – 1491S → A: Impairs phosphorylation by MAPK14, and decreases PIN1-binding. 1 Publication

Organism-specific databases

PharmGKBiPA25451.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 158158Protein BTG2PRO_0000143804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei147 – 1471Phosphoserine; by MAPK1 and MAPK31 Publication
Modified residuei149 – 1491Phosphoserine; by MAPK141 Publication

Post-translational modificationi

Phosphorylated at Ser-147 by MAPK1/ERK2 and MAPK3/ERK1, and at Ser-149 by MAPK14, leading to PIN1-binding and mitochondrial depolarization.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP78543.
PRIDEiP78543.

PTM databases

PhosphoSiteiP78543.

Expressioni

Gene expression databases

BgeeiP78543.
CleanExiHS_BTG2.
GenevestigatoriP78543.

Organism-specific databases

HPAiHPA002355.

Interactioni

Subunit structurei

Interacts with PRKCABP By similarity. Interacts with CNOT7 and CNOT8; indicative for an asscociation with the CCR4-NOT complex. Interacts with PIN1, inducing mitochondrial depolarization.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARP102754EBI-1047576,EBI-608057
CNOT7Q9UIV12EBI-1047576,EBI-2105113
Cnot7Q608095EBI-1047576,EBI-2104739From a different organism.
CNOT8Q9UFF92EBI-1047576,EBI-742299

Protein-protein interaction databases

BioGridi113593. 20 interactions.
IntActiP78543. 5 interactions.
MINTiMINT-155716.
STRINGi9606.ENSP00000290551.

Structurei

Secondary structure

1
158
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 2718
Helixi32 – 5019
Turni59 – 624
Helixi63 – 664
Beta strandi71 – 733
Helixi76 – 849
Helixi89 – 957
Beta strandi100 – 1056
Beta strandi108 – 1147
Beta strandi119 – 1246

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DJUX-ray2.26B7-128[»]
3E9VX-ray1.70A8-127[»]
ProteinModelPortaliP78543.
SMRiP78543. Positions 8-127.

Miscellaneous databases

EvolutionaryTraceiP78543.

Family & Domainsi

Sequence similaritiesi

Belongs to the BTG family.

Phylogenomic databases

eggNOGiNOG287298.
HOGENOMiHOG000290200.
HOVERGENiHBG004907.
InParanoidiP78543.
KOiK14443.
OMAiPSKNYIM.
OrthoDBiEOG72RN0S.
PhylomeDBiP78543.
TreeFamiTF105272.

Family and domain databases

InterProiIPR002087. Anti_prolifrtn.
[Graphical view]
PfamiPF07742. BTG. 1 hit.
[Graphical view]
PRINTSiPR00310. ANTIPRLFBTG1.
SMARTiSM00099. btg1. 1 hit.
[Graphical view]
PROSITEiPS00960. BTG_1. 1 hit.
PS01203. BTG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78543-1 [UniParc]FASTAAdd to Basket

« Hide

MSHGKGTDML PEIAAAVGFL SSLLRTRGCV SEQRLKVFSG ALQEALTEHY    50
KHHWFPEKPS KGSGYRCIRI NHKMDPIISR VASQIGLSQP QLHQLLPSEL 100
TLWVDPYEVS YRIGEDGSIC VLYEEAPLAA SCGLLTCKNQ VLLGRSSPSK 150
NYVMAVSS 158
Length:158
Mass (Da):17,416
Last modified:May 1, 1997 - v1
Checksum:iFFAA1844CC360209
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti153 – 1531V → M.
Corresponds to variant rs12039961 [ dbSNP | Ensembl ].
VAR_048437

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U72649 mRNA. Translation: AAB37580.1.
Y09943 mRNA. Translation: CAA71074.1.
AF361937 Genomic DNA. Translation: AAL05626.1.
AL513326 Genomic DNA. Translation: CAH70451.1.
CH471067 Genomic DNA. Translation: EAW91475.1.
CH471067 Genomic DNA. Translation: EAW91476.1.
BC105948 mRNA. Translation: AAI05949.1.
BC105949 mRNA. Translation: AAI05950.1.
CCDSiCCDS1437.1.
RefSeqiNP_006754.1. NM_006763.2.
UniGeneiHs.519162.

Genome annotation databases

EnsembliENST00000290551; ENSP00000290551; ENSG00000159388.
ENST00000475157; ENSP00000433553; ENSG00000159388.
GeneIDi7832.
KEGGihsa:7832.
UCSCiuc001gzq.3. human.

Polymorphism databases

DMDMi3023409.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U72649 mRNA. Translation: AAB37580.1 .
Y09943 mRNA. Translation: CAA71074.1 .
AF361937 Genomic DNA. Translation: AAL05626.1 .
AL513326 Genomic DNA. Translation: CAH70451.1 .
CH471067 Genomic DNA. Translation: EAW91475.1 .
CH471067 Genomic DNA. Translation: EAW91476.1 .
BC105948 mRNA. Translation: AAI05949.1 .
BC105949 mRNA. Translation: AAI05950.1 .
CCDSi CCDS1437.1.
RefSeqi NP_006754.1. NM_006763.2.
UniGenei Hs.519162.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3DJU X-ray 2.26 B 7-128 [» ]
3E9V X-ray 1.70 A 8-127 [» ]
ProteinModelPortali P78543.
SMRi P78543. Positions 8-127.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113593. 20 interactions.
IntActi P78543. 5 interactions.
MINTi MINT-155716.
STRINGi 9606.ENSP00000290551.

PTM databases

PhosphoSitei P78543.

Polymorphism databases

DMDMi 3023409.

Proteomic databases

PaxDbi P78543.
PRIDEi P78543.

Protocols and materials databases

DNASUi 7832.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000290551 ; ENSP00000290551 ; ENSG00000159388 .
ENST00000475157 ; ENSP00000433553 ; ENSG00000159388 .
GeneIDi 7832.
KEGGi hsa:7832.
UCSCi uc001gzq.3. human.

Organism-specific databases

CTDi 7832.
GeneCardsi GC01P203274.
HGNCi HGNC:1131. BTG2.
HPAi HPA002355.
MIMi 601597. gene.
neXtProti NX_P78543.
PharmGKBi PA25451.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG287298.
HOGENOMi HOG000290200.
HOVERGENi HBG004907.
InParanoidi P78543.
KOi K14443.
OMAi PSKNYIM.
OrthoDBi EOG72RN0S.
PhylomeDBi P78543.
TreeFami TF105272.

Miscellaneous databases

EvolutionaryTracei P78543.
GeneWikii BTG2.
GenomeRNAii 7832.
NextBioi 30230.
PROi P78543.
SOURCEi Search...

Gene expression databases

Bgeei P78543.
CleanExi HS_BTG2.
Genevestigatori P78543.

Family and domain databases

InterProi IPR002087. Anti_prolifrtn.
[Graphical view ]
Pfami PF07742. BTG. 1 hit.
[Graphical view ]
PRINTSi PR00310. ANTIPRLFBTG1.
SMARTi SM00099. btg1. 1 hit.
[Graphical view ]
PROSITEi PS00960. BTG_1. 1 hit.
PS01203. BTG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of BTG2, an antiproliferative p53-dependent component of the DNA damage cellular response pathway."
    Rouault J.-P., Falette N., Guehenneux F., Guillot C., Rimokh R., Wang Q., Berthet C., Moyret-Lalle C., Savatier P., Pain B., Shaw P., Berger R., Samarut J., Magaud J.-P., Ozturk M., Samarut C., Puisieux A.
    Nat. Genet. 14:482-486(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The gene PC3(TIS21/BTG2), prototype member of the PC3/BTG/TOB family: regulator in control of cell growth, differentiation, and DNA repair?"
    Tirone F.
    J. Cell. Physiol. 187:155-165(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fetal liver.
  3. "The human BTG2/TIS21/PC3 gene: genomic structure, transcriptional regulation and evaluation as a candidate tumor suppressor gene."
    Duriez C., Falette N., Audoynaud C., Moyret-Lalle C., Bensaad K., Courtois S., Wang Q., Soussi T., Puisieux A.
    Gene 282:207-214(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Interaction of BTG1 and p53-regulated BTG2 gene products with mCaf1, the murine homolog of a component of the yeast CCR4 transcriptional regulatory complex."
    Rouault J.P., Prevot D., Berthet C., Birot A.M., Billaud M., Magaud J.P., Corbo L.
    J. Biol. Chem. 273:22563-22569(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CNOT7.
  8. "Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway."
    Prevot D., Morel A.P., Voeltzel T., Rostan M.C., Rimokh R., Magaud J.P., Corbo L.
    J. Biol. Chem. 276:9640-9648(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CNOT8.
  9. "BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms."
    Morel A.-P., Sentis S., Bianchin C., Le Romancer M., Jonard L., Rostan M.-C., Rimokh R., Corbo L.
    J. Cell Sci. 116:2929-2936(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE CCR4-NOT COMPLEX.
  10. "Phosphorylation of serine 147 of tis21/BTG2/pc3 by p-Erk1/2 induces Pin-1 binding in cytoplasm and cell death."
    Hong J.W., Ryu M.S., Lim I.K.
    J. Biol. Chem. 280:21256-21263(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-147 AND SER-149, MUTAGENESIS OF SER-147; PRO-148 AND SER-149, INTERACTION WITH PIN1, FUNCTION.
  11. "The BTG2 protein is a general activator of mRNA deadenylation."
    Mauxion F., Faux C., Seraphin B.
    EMBO J. 27:1039-1048(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "PRMT1 and Btg2 regulates neurite outgrowth of Neuro2a cells."
    Miyata S., Mori Y., Tohyama M.
    Neurosci. Lett. 445:162-165(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex."
    Doidge R., Mittal S., Aslam A., Winkler G.S.
    PLoS ONE 7:E51331-E51331(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CNOT7 AND CNOT8, MUTAGENESIS OF HIS-53; TYR-65; ASP-75; TRP-103 AND ASP-105.
  14. "Crystal structures of human BTG2 and mouse TIS21 involved in suppression of CAF1 deadenylase activity."
    Yang X., Morita M., Wang H., Suzuki T., Yang W., Luo Y., Zhao C., Yu Y., Bartlam M., Yamamoto T., Rao Z.
    Nucleic Acids Res. 36:6872-6881(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.26 ANGSTROMS) OF 7-128, INTERACTION WITH CNOT7, MUTAGENESIS OF TYR-65; TRP-103 AND GLU-115.

Entry informationi

Entry nameiBTG2_HUMAN
AccessioniPrimary (citable) accession number: P78543
Secondary accession number(s): A0A024R986, Q3KR25, Q5VUT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: September 3, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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