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Protein

Protein BTG2

Gene

BTG2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Activates mRNA deadenylation in a CNOT6 and CNOT7-dependent manner. In vitro can inhibit deadenylase activity of CNOT7 and CNOT8. Involved in cell cycle regulation. Could be involved in the growth arrest and differentiation of the neuronal precursors (By similarity). Modulates transcription regulation mediated by ESR1. Involved in mitochondrial depolarization and neurite outgrowth.By similarity5 Publications

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl

GO - Biological processi

  1. anterior/posterior pattern specification Source: Ensembl
  2. associative learning Source: Ensembl
  3. cellular response to DNA damage stimulus Source: MGI
  4. central nervous system neuron development Source: Ensembl
  5. dentate gyrus development Source: Ensembl
  6. DNA repair Source: ProtInc
  7. negative regulation of cell proliferation Source: UniProtKB
  8. negative regulation of neural precursor cell proliferation Source: Ensembl
  9. negative regulation of neuron apoptotic process Source: Ensembl
  10. negative regulation of translation Source: UniProtKB
  11. neuron projection development Source: UniProtKB
  12. positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: MGI
  13. protein methylation Source: Ensembl
  14. response to electrical stimulus Source: Ensembl
  15. response to mechanical stimulus Source: Ensembl
  16. response to organic cyclic compound Source: Ensembl
  17. response to peptide hormone Source: Ensembl
  18. skeletal muscle cell differentiation Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein BTG2
Alternative name(s):
BTG family member 2
NGF-inducible anti-proliferative protein PC3
Gene namesi
Name:BTG2
Synonyms:PC3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:1131. BTG2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi53 – 531H → A: Impairs interaction with CNOT7 and CNOT8. 1 Publication
Mutagenesisi65 – 651Y → A: Abolishes interaction with CNOT7 and CNOT8. 2 Publications
Mutagenesisi75 – 751D → A: Abolishes interaction with CNOT7 and CNOT8. 1 Publication
Mutagenesisi103 – 1031W → A: Abolishes interaction with CNOT7 and CNOT8; impairs anti-proliferative activity. 2 Publications
Mutagenesisi105 – 1051D → A: Impairs interaction with CNOT7 and CNOT8. 1 Publication
Mutagenesisi115 – 1151E → A: Impairs interaction with CNOT7. Inhibits CNOT7 mRNA deadenylase activity. 1 Publication
Mutagenesisi147 – 1471S → A: Impairs phosphorylation by MAPK1 and MAPK3, and decreases PIN1-binding. 1 Publication
Mutagenesisi148 – 1481P → A: Impairs PIN1-binding. 1 Publication
Mutagenesisi149 – 1491S → A: Impairs phosphorylation by MAPK14, and decreases PIN1-binding. 1 Publication

Organism-specific databases

PharmGKBiPA25451.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 158158Protein BTG2PRO_0000143804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei147 – 1471Phosphoserine; by MAPK1 and MAPK31 Publication
Modified residuei149 – 1491Phosphoserine; by MAPK141 Publication

Post-translational modificationi

Phosphorylated at Ser-147 by MAPK1/ERK2 and MAPK3/ERK1, and at Ser-149 by MAPK14, leading to PIN1-binding and mitochondrial depolarization.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP78543.
PaxDbiP78543.
PRIDEiP78543.

PTM databases

PhosphoSiteiP78543.

Expressioni

Gene expression databases

BgeeiP78543.
CleanExiHS_BTG2.
GenevestigatoriP78543.

Organism-specific databases

HPAiHPA002355.

Interactioni

Subunit structurei

Interacts with PRKCABP (By similarity). Interacts with CNOT7 and CNOT8; indicative for an asscociation with the CCR4-NOT complex. Interacts with PIN1, inducing mitochondrial depolarization.By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARP102754EBI-1047576,EBI-608057
CNOT7Q9UIV12EBI-1047576,EBI-2105113
Cnot7Q608095EBI-1047576,EBI-2104739From a different organism.
CNOT8Q9UFF92EBI-1047576,EBI-742299

Protein-protein interaction databases

BioGridi113593. 20 interactions.
IntActiP78543. 5 interactions.
MINTiMINT-155716.
STRINGi9606.ENSP00000290551.

Structurei

Secondary structure

1
158
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 2718Combined sources
Helixi32 – 5019Combined sources
Turni59 – 624Combined sources
Helixi63 – 664Combined sources
Beta strandi71 – 733Combined sources
Helixi76 – 849Combined sources
Helixi89 – 957Combined sources
Beta strandi100 – 1056Combined sources
Beta strandi108 – 1147Combined sources
Beta strandi119 – 1246Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DJUX-ray2.26B7-128[»]
3E9VX-ray1.70A8-127[»]
ProteinModelPortaliP78543.
SMRiP78543. Positions 8-127.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78543.

Family & Domainsi

Sequence similaritiesi

Belongs to the BTG family.Curated

Phylogenomic databases

eggNOGiNOG287298.
GeneTreeiENSGT00550000074461.
HOGENOMiHOG000290200.
HOVERGENiHBG004907.
InParanoidiP78543.
KOiK14443.
OMAiMLTCKNQ.
OrthoDBiEOG72RN0S.
PhylomeDBiP78543.
TreeFamiTF105272.

Family and domain databases

InterProiIPR002087. Anti_prolifrtn.
[Graphical view]
PfamiPF07742. BTG. 1 hit.
[Graphical view]
PRINTSiPR00310. ANTIPRLFBTG1.
SMARTiSM00099. btg1. 1 hit.
[Graphical view]
PROSITEiPS00960. BTG_1. 1 hit.
PS01203. BTG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHGKGTDML PEIAAAVGFL SSLLRTRGCV SEQRLKVFSG ALQEALTEHY
60 70 80 90 100
KHHWFPEKPS KGSGYRCIRI NHKMDPIISR VASQIGLSQP QLHQLLPSEL
110 120 130 140 150
TLWVDPYEVS YRIGEDGSIC VLYEEAPLAA SCGLLTCKNQ VLLGRSSPSK

NYVMAVSS
Length:158
Mass (Da):17,416
Last modified:May 1, 1997 - v1
Checksum:iFFAA1844CC360209
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti153 – 1531V → M.
Corresponds to variant rs12039961 [ dbSNP | Ensembl ].
VAR_048437

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72649 mRNA. Translation: AAB37580.1.
Y09943 mRNA. Translation: CAA71074.1.
AF361937 Genomic DNA. Translation: AAL05626.1.
AL513326 Genomic DNA. Translation: CAH70451.1.
CH471067 Genomic DNA. Translation: EAW91475.1.
CH471067 Genomic DNA. Translation: EAW91476.1.
BC105948 mRNA. Translation: AAI05949.1.
BC105949 mRNA. Translation: AAI05950.1.
CCDSiCCDS1437.1.
RefSeqiNP_006754.1. NM_006763.2.
UniGeneiHs.519162.

Genome annotation databases

EnsembliENST00000290551; ENSP00000290551; ENSG00000159388.
ENST00000475157; ENSP00000433553; ENSG00000159388.
GeneIDi7832.
KEGGihsa:7832.
UCSCiuc001gzq.3. human.

Polymorphism databases

DMDMi3023409.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72649 mRNA. Translation: AAB37580.1.
Y09943 mRNA. Translation: CAA71074.1.
AF361937 Genomic DNA. Translation: AAL05626.1.
AL513326 Genomic DNA. Translation: CAH70451.1.
CH471067 Genomic DNA. Translation: EAW91475.1.
CH471067 Genomic DNA. Translation: EAW91476.1.
BC105948 mRNA. Translation: AAI05949.1.
BC105949 mRNA. Translation: AAI05950.1.
CCDSiCCDS1437.1.
RefSeqiNP_006754.1. NM_006763.2.
UniGeneiHs.519162.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DJUX-ray2.26B7-128[»]
3E9VX-ray1.70A8-127[»]
ProteinModelPortaliP78543.
SMRiP78543. Positions 8-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113593. 20 interactions.
IntActiP78543. 5 interactions.
MINTiMINT-155716.
STRINGi9606.ENSP00000290551.

PTM databases

PhosphoSiteiP78543.

Polymorphism databases

DMDMi3023409.

Proteomic databases

MaxQBiP78543.
PaxDbiP78543.
PRIDEiP78543.

Protocols and materials databases

DNASUi7832.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000290551; ENSP00000290551; ENSG00000159388.
ENST00000475157; ENSP00000433553; ENSG00000159388.
GeneIDi7832.
KEGGihsa:7832.
UCSCiuc001gzq.3. human.

Organism-specific databases

CTDi7832.
GeneCardsiGC01P203274.
HGNCiHGNC:1131. BTG2.
HPAiHPA002355.
MIMi601597. gene.
neXtProtiNX_P78543.
PharmGKBiPA25451.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG287298.
GeneTreeiENSGT00550000074461.
HOGENOMiHOG000290200.
HOVERGENiHBG004907.
InParanoidiP78543.
KOiK14443.
OMAiMLTCKNQ.
OrthoDBiEOG72RN0S.
PhylomeDBiP78543.
TreeFamiTF105272.

Miscellaneous databases

ChiTaRSiBTG2. human.
EvolutionaryTraceiP78543.
GeneWikiiBTG2.
GenomeRNAii7832.
NextBioi30230.
PROiP78543.
SOURCEiSearch...

Gene expression databases

BgeeiP78543.
CleanExiHS_BTG2.
GenevestigatoriP78543.

Family and domain databases

InterProiIPR002087. Anti_prolifrtn.
[Graphical view]
PfamiPF07742. BTG. 1 hit.
[Graphical view]
PRINTSiPR00310. ANTIPRLFBTG1.
SMARTiSM00099. btg1. 1 hit.
[Graphical view]
PROSITEiPS00960. BTG_1. 1 hit.
PS01203. BTG_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of BTG2, an antiproliferative p53-dependent component of the DNA damage cellular response pathway."
    Rouault J.-P., Falette N., Guehenneux F., Guillot C., Rimokh R., Wang Q., Berthet C., Moyret-Lalle C., Savatier P., Pain B., Shaw P., Berger R., Samarut J., Magaud J.-P., Ozturk M., Samarut C., Puisieux A.
    Nat. Genet. 14:482-486(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The gene PC3(TIS21/BTG2), prototype member of the PC3/BTG/TOB family: regulator in control of cell growth, differentiation, and DNA repair?"
    Tirone F.
    J. Cell. Physiol. 187:155-165(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fetal liver.
  3. "The human BTG2/TIS21/PC3 gene: genomic structure, transcriptional regulation and evaluation as a candidate tumor suppressor gene."
    Duriez C., Falette N., Audoynaud C., Moyret-Lalle C., Bensaad K., Courtois S., Wang Q., Soussi T., Puisieux A.
    Gene 282:207-214(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Interaction of BTG1 and p53-regulated BTG2 gene products with mCaf1, the murine homolog of a component of the yeast CCR4 transcriptional regulatory complex."
    Rouault J.P., Prevot D., Berthet C., Birot A.M., Billaud M., Magaud J.P., Corbo L.
    J. Biol. Chem. 273:22563-22569(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CNOT7.
  8. "Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway."
    Prevot D., Morel A.P., Voeltzel T., Rostan M.C., Rimokh R., Magaud J.P., Corbo L.
    J. Biol. Chem. 276:9640-9648(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CNOT8.
  9. "BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms."
    Morel A.-P., Sentis S., Bianchin C., Le Romancer M., Jonard L., Rostan M.-C., Rimokh R., Corbo L.
    J. Cell Sci. 116:2929-2936(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE CCR4-NOT COMPLEX.
  10. "Phosphorylation of serine 147 of tis21/BTG2/pc3 by p-Erk1/2 induces Pin-1 binding in cytoplasm and cell death."
    Hong J.W., Ryu M.S., Lim I.K.
    J. Biol. Chem. 280:21256-21263(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-147 AND SER-149, MUTAGENESIS OF SER-147; PRO-148 AND SER-149, INTERACTION WITH PIN1, FUNCTION.
  11. "The BTG2 protein is a general activator of mRNA deadenylation."
    Mauxion F., Faux C., Seraphin B.
    EMBO J. 27:1039-1048(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "PRMT1 and Btg2 regulates neurite outgrowth of Neuro2a cells."
    Miyata S., Mori Y., Tohyama M.
    Neurosci. Lett. 445:162-165(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex."
    Doidge R., Mittal S., Aslam A., Winkler G.S.
    PLoS ONE 7:E51331-E51331(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CNOT7 AND CNOT8, MUTAGENESIS OF HIS-53; TYR-65; ASP-75; TRP-103 AND ASP-105.
  14. "Crystal structures of human BTG2 and mouse TIS21 involved in suppression of CAF1 deadenylase activity."
    Yang X., Morita M., Wang H., Suzuki T., Yang W., Luo Y., Zhao C., Yu Y., Bartlam M., Yamamoto T., Rao Z.
    Nucleic Acids Res. 36:6872-6881(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.26 ANGSTROMS) OF 7-128, INTERACTION WITH CNOT7, MUTAGENESIS OF TYR-65; TRP-103 AND GLU-115.

Entry informationi

Entry nameiBTG2_HUMAN
AccessioniPrimary (citable) accession number: P78543
Secondary accession number(s): A0A024R986, Q3KR25, Q5VUT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: March 4, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.