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Protein

Suppression of tumorigenicity 5 protein

Gene

ST5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May be involved in cytoskeletal organization and tumorogenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth.3 Publications

GO - Molecular functioni

  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  • positive regulation of ERK1 and ERK2 cascade Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Suppression of tumorigenicity 5 protein
Alternative name(s):
DENN domain-containing protein 2B
HeLa tumor suppression 1
Gene namesi
Name:ST5
Synonyms:DENND2B, HTS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:11350. ST5.

Pathology & Biotechi

Organism-specific databases

DisGeNETi6764.
OpenTargetsiENSG00000166444.
PharmGKBiPA36172.

Polymorphism and mutation databases

BioMutaiST5.
DMDMi317373507.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002474481 – 1137Suppression of tumorigenicity 5 proteinAdd BLAST1137

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphoserineBy similarity1
Modified residuei231PhosphothreonineBy similarity1
Modified residuei233PhosphoserineBy similarity1
Modified residuei364PhosphothreonineCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei482PhosphothreonineBy similarity1
Modified residuei545PhosphoserineBy similarity1
Modified residuei574PhosphoserineBy similarity1
Modified residuei622PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP78524.
MaxQBiP78524.
PaxDbiP78524.
PeptideAtlasiP78524.
PRIDEiP78524.

PTM databases

iPTMnetiP78524.
PhosphoSitePlusiP78524.

Expressioni

Tissue specificityi

Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000166444.
CleanExiHS_ST5.
ExpressionAtlasiP78524. baseline and differential.
GenevisibleiP78524. HS.

Organism-specific databases

HPAiHPA046796.

Interactioni

Subunit structurei

Isoform 1 interacts with the SH3 domain of ABL1.1 Publication

Protein-protein interaction databases

BioGridi112642. 16 interactors.
IntActiP78524. 18 interactors.
MINTiMINT-1184327.
STRINGi9606.ENSP00000319678.

Structurei

3D structure databases

ProteinModelPortaliP78524.
SMRiP78524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini709 – 784UDENNAdd BLAST76
Domaini791 – 975DENNPROSITE-ProRule annotationAdd BLAST185
Domaini1022 – 1089dDENNPROSITE-ProRule annotationAdd BLAST68

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni401 – 447Interaction with ABL11 PublicationAdd BLAST47

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi33 – 93Pro-richAdd BLAST61
Compositional biasi350 – 434Pro-richAdd BLAST85

Sequence similaritiesi

Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3569. Eukaryota.
ENOG410XT3N. LUCA.
GeneTreeiENSGT00760000118819.
HOVERGENiHBG079980.
InParanoidiP78524.
KOiK20161.
OMAiDRKYNNP.
OrthoDBiEOG091G01KD.
PhylomeDBiP78524.
TreeFamiTF320336.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P78524-1) [UniParc]FASTAAdd to basket
Also known as: p126

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMTANKNSS ITHGAGGTKA PRGTLSRSQS VSPPPVLSPP RSPIYPLSDS
60 70 80 90 100
ETSACRYPSH SSSRVLLKDR HPPAPSPQNP QDPSPDTSPP TCPFKTASFG
110 120 130 140 150
YLDRSPSACK RDAQKESVQG AAQDVAGVAA CLPLAQSTPF PGPAAGPRGV
160 170 180 190 200
LLTRTGTRAH SLGIREKISA WEGRREASPR MSMCGEKREG SGSEWAASEG
210 220 230 240 250
CPSLGCPSVV PSPCSSEKTF DFKGLRRMSR TFSECSYPET EEEGEALPVR
260 270 280 290 300
DSFYRLEKRL GRSEPSAFLR GHGSRKESSA VLSRIQKIEQ VLKEQPGRGL
310 320 330 340 350
PQLPSSCYSV DRGKRKTGTL GSLEEPAGGA SVSAGSRAVG VAGVAGEAGP
360 370 380 390 400
PPEREGSGST KPGTPGNSPS SQRLPSKSSL DPAVNPVPKP KRTFEYEADK
410 420 430 440 450
NPKSKPSNGL PPSPTPAAPP PLPSTPAPPV TRRPKKDMRG HRKSQSRKSF
460 470 480 490 500
EFEDASSLQS LYPSSPTENG TENQPKFGSK STLEENAYED IVGDLPKENP
510 520 530 540 550
YEDVDLKSRR AGRKSQQLSE NSLDSLHRMW SPQDRKYNSP PTQLSLKPNS
560 570 580 590 600
QSLRSGNWSE RKSHRLPRLP KRHSHDDMLL LAQLSLPSSP SSLNEDSLST
610 620 630 640 650
TSELLSSRRA RRIPKLVQRI NSIYNAKRGK KRLKKLSMSS IETASLRDEN
660 670 680 690 700
SESESDSDDR FKAHTQRLVH IQSMLKRAPS YRTLELELLE WQERELFEYF
710 720 730 740 750
VVVSLKKKPS RNTYLPEVSY QFPKLDRPTK QMREAEERLK AIPQFCFPDA
760 770 780 790 800
KDWLPVSEYS SETFSFMLTG EDGSRRFGYC RRLLPSGKGP RLPEVYCVIS
810 820 830 840 850
RLGCFGLFSK VLDEVERRRG ISAALVYPFM RSLMESPFPA PGKTIKVKTF
860 870 880 890 900
LPGAGNEVLE LRRPMDSRLE HVDFECLFTC LSVRQLIRIF ASLLLERRVI
910 920 930 940 950
FVADKLSTLS SCSHAVVALL YPFSWQHTFI PVLPASMIDI VCCPTPFLVG
960 970 980 990 1000
LLSSSLPKLK ELPVEEALMV NLGSDRFIRQ MDDEDTLLPR KLQAALEQAL
1010 1020 1030 1040 1050
ERKNELISQD SDSDSDDECN TLNGLVSEVF IRFFVETVGH YSLFLTQSEK
1060 1070 1080 1090 1100
GERAFQREAF RKSVASKSIR RFLEVFMESQ MFAGFIQDRE LRKCRAKGLF
1110 1120 1130
EQRVEQYLEE LPDTEQSGMN KFLRGLGNKM KFLHKKN
Note: Produced by alternative promoter usage.
Length:1,137
Mass (Da):126,485
Last modified:January 11, 2011 - v3
Checksum:i96ACD16E0602479E
GO
Isoform 2 (identifier: P78524-2) [UniParc]FASTAAdd to basket
Also known as: p82

The sequence of this isoform differs from the canonical sequence as follows:
     28-447: Missing.

Note: Produced by alternative splicing of isoform 1.
Show »
Length:717
Mass (Da):82,079
Checksum:i0329586AEE3E05BE
GO
Isoform 3 (identifier: P78524-3) [UniParc]FASTAAdd to basket
Also known as: p70

The sequence of this isoform differs from the canonical sequence as follows:
     1-528: Missing.

Note: Produced by alternative promoter usage.
Show »
Length:609
Mass (Da):70,245
Checksum:i59F8FB669B1058E5
GO

Sequence cautioni

The sequence AAC50925 differs from that shown. Reason: Frameshift at positions 159 and 169.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113A → T in AAC50925 (PubMed:8972856).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027101316K → N.1 PublicationCorresponds to variant rs3794153dbSNPEnsembl.1
Natural variantiVAR_027102399D → E.2 PublicationsCorresponds to variant rs3812762dbSNPEnsembl.1
Natural variantiVAR_027103620I → T.1 PublicationCorresponds to variant rs17853683dbSNPEnsembl.1
Natural variantiVAR_030642657S → F.Corresponds to variant rs11042047dbSNPEnsembl.1
Natural variantiVAR_027104774S → G.1 PublicationCorresponds to variant rs17853682dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0199871 – 528Missing in isoform 3. 2 PublicationsAdd BLAST528
Alternative sequenceiVSP_01998828 – 447Missing in isoform 2. 2 PublicationsAdd BLAST420

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15131 mRNA. Translation: AAC50925.1. Frameshift.
U15779 mRNA. Translation: AAB97097.1.
U15780 mRNA. Translation: AAC50926.1.
AJ400879 Genomic DNA. Translation: CAC35387.1.
AK127763 mRNA. Translation: BAG54568.1.
AK312758 mRNA. Translation: BAG35624.1.
AC026894 Genomic DNA. No translation available.
AC091053 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68618.1.
BC036655 mRNA. Translation: AAH36655.1.
S45936 mRNA. Translation: AAB23647.1.
CCDSiCCDS7791.1. [P78524-1]
CCDS7792.1. [P78524-2]
PIRiA49013.
RefSeqiNP_005409.3. NM_005418.3. [P78524-1]
NP_631896.1. NM_139157.2. [P78524-2]
NP_998783.1. NM_213618.1. [P78524-1]
XP_005253140.1. XM_005253083.2. [P78524-2]
XP_011518620.1. XM_011520318.1. [P78524-1]
XP_011518631.1. XM_011520329.1. [P78524-2]
UniGeneiHs.117715.

Genome annotation databases

EnsembliENST00000313726; ENSP00000319678; ENSG00000166444. [P78524-1]
ENST00000526757; ENSP00000435097; ENSG00000166444. [P78524-2]
ENST00000530438; ENSP00000436802; ENSG00000166444. [P78524-2]
ENST00000530991; ENSP00000432887; ENSG00000166444. [P78524-3]
ENST00000534127; ENSP00000433528; ENSG00000166444. [P78524-1]
GeneIDi6764.
KEGGihsa:6764.
UCSCiuc001mgt.4. human. [P78524-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15131 mRNA. Translation: AAC50925.1. Frameshift.
U15779 mRNA. Translation: AAB97097.1.
U15780 mRNA. Translation: AAC50926.1.
AJ400879 Genomic DNA. Translation: CAC35387.1.
AK127763 mRNA. Translation: BAG54568.1.
AK312758 mRNA. Translation: BAG35624.1.
AC026894 Genomic DNA. No translation available.
AC091053 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68618.1.
BC036655 mRNA. Translation: AAH36655.1.
S45936 mRNA. Translation: AAB23647.1.
CCDSiCCDS7791.1. [P78524-1]
CCDS7792.1. [P78524-2]
PIRiA49013.
RefSeqiNP_005409.3. NM_005418.3. [P78524-1]
NP_631896.1. NM_139157.2. [P78524-2]
NP_998783.1. NM_213618.1. [P78524-1]
XP_005253140.1. XM_005253083.2. [P78524-2]
XP_011518620.1. XM_011520318.1. [P78524-1]
XP_011518631.1. XM_011520329.1. [P78524-2]
UniGeneiHs.117715.

3D structure databases

ProteinModelPortaliP78524.
SMRiP78524.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112642. 16 interactors.
IntActiP78524. 18 interactors.
MINTiMINT-1184327.
STRINGi9606.ENSP00000319678.

PTM databases

iPTMnetiP78524.
PhosphoSitePlusiP78524.

Polymorphism and mutation databases

BioMutaiST5.
DMDMi317373507.

Proteomic databases

EPDiP78524.
MaxQBiP78524.
PaxDbiP78524.
PeptideAtlasiP78524.
PRIDEiP78524.

Protocols and materials databases

DNASUi6764.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313726; ENSP00000319678; ENSG00000166444. [P78524-1]
ENST00000526757; ENSP00000435097; ENSG00000166444. [P78524-2]
ENST00000530438; ENSP00000436802; ENSG00000166444. [P78524-2]
ENST00000530991; ENSP00000432887; ENSG00000166444. [P78524-3]
ENST00000534127; ENSP00000433528; ENSG00000166444. [P78524-1]
GeneIDi6764.
KEGGihsa:6764.
UCSCiuc001mgt.4. human. [P78524-1]

Organism-specific databases

CTDi6764.
DisGeNETi6764.
GeneCardsiST5.
H-InvDBHIX0026155.
HGNCiHGNC:11350. ST5.
HPAiHPA046796.
MIMi140750. gene.
neXtProtiNX_P78524.
OpenTargetsiENSG00000166444.
PharmGKBiPA36172.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3569. Eukaryota.
ENOG410XT3N. LUCA.
GeneTreeiENSGT00760000118819.
HOVERGENiHBG079980.
InParanoidiP78524.
KOiK20161.
OMAiDRKYNNP.
OrthoDBiEOG091G01KD.
PhylomeDBiP78524.
TreeFamiTF320336.

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiST5. human.
GeneWikiiST5_(gene).
GenomeRNAii6764.
PROiP78524.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166444.
CleanExiHS_ST5.
ExpressionAtlasiP78524. baseline and differential.
GenevisibleiP78524. HS.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiST5_HUMAN
AccessioniPrimary (citable) accession number: P78524
Secondary accession number(s): B2R6X7
, B3KXQ6, P78523, Q16492, Q7KYY2, Q7KZ12, Q8NE12, Q9BQQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.