Suppression of tumorigenicity 5 - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    Suppression of tumorigenicity 5
Alternative name(s):
    HeLa tumor suppression 1
    DENN domain-containing protein 2B

Gene names

Name:	ST5
Synonyms:	DENND2B, HTS1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	1137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May be involved in cytoskeletal organization and tumorgenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth.
Subunit structure	Isoform 1 interacts with the SH3 domain of ABL1.
Tissue specificity	Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level).
Sequence similarities	Contains 1 dDENN domain. Contains 1 DENN domain. Contains 1 uDENN domain.

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Ontologies

Keywords
Coding sequence diversity	Alternative promoter usage Alternative splicing Polymorphism
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular function	protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABL1	P00519	1	EBI-962633,EBI-375543
GRB2	P62993	1	EBI-962633,EBI-401755

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Alternative products

This entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]

Isoform 1 (identifier: P78524-1) Also known as: p126; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Notes: Produced by alternative promoter usage.

Isoform 2 (identifier: P78524-2) Also known as: p82; The sequence of this isoform differs from the canonical sequence as follows: 28-447: Missing.
Notes: Produced by alternative splicing of isoform 1.

Isoform 3 (identifier: P78524-3) Also known as: p70; The sequence of this isoform differs from the canonical sequence as follows: 1-528: Missing.
Notes: Produced by alternative promoter usage.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1137

1137

Suppression of tumorigenicity 5

PRO_0000247448

Regions

Domain

693 – 790

98

uDENN

Domain

791 – 975

185

DENN

Domain

1022 – 1089

68

dDENN

Region

401 – 447

47

Interaction with ABL1

Compositional bias

33 – 93

61

Pro-rich

Compositional bias

350 – 434

85

Pro-rich

Amino acid modifications

Modified residue

622

1

Phosphoserine

Modified residue

1011

1

Phosphoserine

Modified residue

1013

1

Phosphoserine

Modified residue

1015

1

Phosphoserine

Natural variations

Alternative sequence

1 – 528

528

Missing in isoform 3.

VSP_019987

Alternative sequence

28 – 447

420

Missing in isoform 2.

VSP_019988

Natural variant

316

1

K → N: dbSNP rs3794153.

VAR_027101

Natural variant

399

1

E → D: dbSNP rs3812762.

VAR_027102

Natural variant

620

1

I → T: dbSNP rs17853683.

VAR_027103

Natural variant

657

1

S → F: dbSNP rs11042047.

VAR_030642

Natural variant

774

1

S → G: dbSNP rs17853682.

VAR_027104

Experimental info

Sequence conflict

113

1

A → T in AAC50925, AAB97097 and AAC50926. Ref.1

Sequence conflict

159 – 169

11

AHSLGIREKIS → SPQPGHPGEDI in AAC50925, AAB97097 and AAC50926. Ref.1

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 (p126) [UniParc]. Last modified July 25, 2006. Version 2. Checksum: B8573EC3349CC4F4 Show »	FASTA	1,137	126,499
10 20 30 40 50 60 MTMTANKNSS ITHGAGGTKA PRGTLSRSQS VSPPPVLSPP RSPIYPLSDS ETSACRYPSH 70 80 90 100 110 120 SSSRVLLKDR HPPAPSPQNP QDPSPDTSPP TCPFKTASFG YLDRSPSACK RDAQKESVQG 130 140 150 160 170 180 AAQDVAGVAA CLPLAQSTPF PGPAAGPRGV LLTRTGTRAH SLGIREKISA WEGRREASPR 190 200 210 220 230 240 MSMCGEKREG SGSEWAASEG CPSLGCPSVV PSPCSSEKTF DFKGLRRMSR TFSECSYPET 250 260 270 280 290 300 EEEGEALPVR DSFYRLEKRL GRSEPSAFLR GHGSRKESSA VLSRIQKIEQ VLKEQPGRGL 310 320 330 340 350 360 PQLPSSCYSV DRGKRKTGTL GSLEEPAGGA SVSAGSRAVG VAGVAGEAGP PPEREGSGST 370 380 390 400 410 420 KPGTPGNSPS SQRLPSKSSL DPAVNPVPKP KRTFEYEAEK NPKSKPSNGL PPSPTPAAPP 430 440 450 460 470 480 PLPSTPAPPV TRRPKKDMRG HRKSQSRKSF EFEDASSLQS LYPSSPTENG TENQPKFGSK 490 500 510 520 530 540 STLEENAYED IVGDLPKENP YEDVDLKSRR AGRKSQQLSE NSLDSLHRMW SPQDRKYNSP 550 560 570 580 590 600 PTQLSLKPNS QSLRSGNWSE RKSHRLPRLP KRHSHDDMLL LAQLSLPSSP SSLNEDSLST 610 620 630 640 650 660 TSELLSSRRA RRIPKLVQRI NSIYNAKRGK KRLKKLSMSS IETASLRDEN SESESDSDDR 670 680 690 700 710 720 FKAHTQRLVH IQSMLKRAPS YRTLELELLE WQERELFEYF VVVSLKKKPS RNTYLPEVSY 730 740 750 760 770 780 QFPKLDRPTK QMREAEERLK AIPQFCFPDA KDWLPVSEYS SETFSFMLTG EDGSRRFGYC 790 800 810 820 830 840 RRLLPSGKGP RLPEVYCVIS RLGCFGLFSK VLDEVERRRG ISAALVYPFM RSLMESPFPA 850 860 870 880 890 900 PGKTIKVKTF LPGAGNEVLE LRRPMDSRLE HVDFECLFTC LSVRQLIRIF ASLLLERRVI 910 920 930 940 950 960 FVADKLSTLS SCSHAVVALL YPFSWQHTFI PVLPASMIDI VCCPTPFLVG LLSSSLPKLK 970 980 990 1000 1010 1020 ELPVEEALMV NLGSDRFIRQ MDDEDTLLPR KLQAALEQAL ERKNELISQD SDSDSDDECN 1030 1040 1050 1060 1070 1080 TLNGLVSEVF IRFFVETVGH YSLFLTQSEK GERAFQREAF RKSVASKSIR RFLEVFMESQ 1090 1100 1110 1120 1130 MFAGFIQDRE LRKCRAKGLF EQRVEQYLEE LPDTEQSGMN KFLRGLGNKM KFLHKKN « Hide
Isoform 2 (p82) [UniParc]. Checksum: 0329586AEE3E05BE Show »		717	82,079
10 20 30 40 50 60 MTMTANKNSS ITHGAGGTKA PRGTLSRKSF EFEDASSLQS LYPSSPTENG TENQPKFGSK 70 80 90 100 110 120 STLEENAYED IVGDLPKENP YEDVDLKSRR AGRKSQQLSE NSLDSLHRMW SPQDRKYNSP 130 140 150 160 170 180 PTQLSLKPNS QSLRSGNWSE RKSHRLPRLP KRHSHDDMLL LAQLSLPSSP SSLNEDSLST 190 200 210 220 230 240 TSELLSSRRA RRIPKLVQRI NSIYNAKRGK KRLKKLSMSS IETASLRDEN SESESDSDDR 250 260 270 280 290 300 FKAHTQRLVH IQSMLKRAPS YRTLELELLE WQERELFEYF VVVSLKKKPS RNTYLPEVSY 310 320 330 340 350 360 QFPKLDRPTK QMREAEERLK AIPQFCFPDA KDWLPVSEYS SETFSFMLTG EDGSRRFGYC 370 380 390 400 410 420 RRLLPSGKGP RLPEVYCVIS RLGCFGLFSK VLDEVERRRG ISAALVYPFM RSLMESPFPA 430 440 450 460 470 480 PGKTIKVKTF LPGAGNEVLE LRRPMDSRLE HVDFECLFTC LSVRQLIRIF ASLLLERRVI 490 500 510 520 530 540 FVADKLSTLS SCSHAVVALL YPFSWQHTFI PVLPASMIDI VCCPTPFLVG LLSSSLPKLK 550 560 570 580 590 600 ELPVEEALMV NLGSDRFIRQ MDDEDTLLPR KLQAALEQAL ERKNELISQD SDSDSDDECN 610 620 630 640 650 660 TLNGLVSEVF IRFFVETVGH YSLFLTQSEK GERAFQREAF RKSVASKSIR RFLEVFMESQ 670 680 690 700 710 MFAGFIQDRE LRKCRAKGLF EQRVEQYLEE LPDTEQSGMN KFLRGLGNKM KFLHKKN « Hide
Isoform 3 (p70) [UniParc]. Checksum: 59F8FB669B1058E5 Show »		609	70,245
10 20 30 40 50 60 MWSPQDRKYN SPPTQLSLKP NSQSLRSGNW SERKSHRLPR LPKRHSHDDM LLLAQLSLPS 70 80 90 100 110 120 SPSSLNEDSL STTSELLSSR RARRIPKLVQ RINSIYNAKR GKKRLKKLSM SSIETASLRD 130 140 150 160 170 180 ENSESESDSD DRFKAHTQRL VHIQSMLKRA PSYRTLELEL LEWQERELFE YFVVVSLKKK 190 200 210 220 230 240 PSRNTYLPEV SYQFPKLDRP TKQMREAEER LKAIPQFCFP DAKDWLPVSE YSSETFSFML 250 260 270 280 290 300 TGEDGSRRFG YCRRLLPSGK GPRLPEVYCV ISRLGCFGLF SKVLDEVERR RGISAALVYP 310 320 330 340 350 360 FMRSLMESPF PAPGKTIKVK TFLPGAGNEV LELRRPMDSR LEHVDFECLF TCLSVRQLIR 370 380 390 400 410 420 IFASLLLERR VIFVADKLST LSSCSHAVVA LLYPFSWQHT FIPVLPASMI DIVCCPTPFL 430 440 450 460 470 480 VGLLSSSLPK LKELPVEEAL MVNLGSDRFI RQMDDEDTLL PRKLQAALEQ ALERKNELIS 490 500 510 520 530 540 QDSDSDSDDE CNTLNGLVSE VFIRFFVETV GHYSLFLTQS EKGERAFQRE AFRKSVASKS 550 560 570 580 590 600 IRRFLEVFME SQMFAGFIQD RELRKCRAKG LFEQRVEQYL EELPDTEQSG MNKFLRGLGN KMKFLHKKN « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential expression of the human ST5 gene in HeLa-fibroblast hybrid cell lines mediated by YY1: evidence that YY1 plays a part in tumor suppression." Lichy J.H., Majidi M., Elbaum J., Tsai M.M. Nucleic Acids Res. 24:4700-4708(1996) [PubMed: 8972856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
[2]	"Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7." Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A., Zabel B., Hankeln T., Schmidt E.R. Cytogenet. Cell Genet. 93:284-290(2001) [PubMed: 11528127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ASN-316; ASP-399; THR-620 AND GLY-774. Tissue: Testis.
[4]	"Identification of a human chromosome 11 gene which is differentially regulated in tumorigenic and nontumorigenic somatic cell hybrids of HeLa cells." Lichy J.H., Modi W.S., Seuanez H.N., Howley P.M. Cell Growth Differ. 3:541-548(1992) [PubMed: 1390339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 493-1137.
[5]	"Activation of extracellular signal-regulated kinase 2 by a novel Abl-binding protein, ST5." Majidi M., Hubbs A.E., Lichy J.H. J. Biol. Chem. 273:16608-16614(1998) [PubMed: 9632734] [Abstract] Cited for: FUNCTION, INTERACTION WITH ABL1.
[6]	"Expression of an isoform of the novel signal transduction protein ST5 is linked to cell morphology." Hubbs A.E., Majidi M., Lichy J.H. Oncogene 18:2519-2525(1999) [PubMed: 10229203] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1011; SER-1013 AND SER-1015, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-622, MASS SPECTROMETRY.

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Cross-references

Sequence databases
	U15131 mRNA. Translation: AAC50925.1. U15779 mRNA. Translation: AAB97097.1. U15780 mRNA. Translation: AAC50926.1. AJ400879 Genomic DNA. Translation: CAC35387.1. BC036655 mRNA. Translation: AAH36655.1. S45936 mRNA. Translation: AAB23647.1.
PIR	A49013.
RefSeq	NP_005409.3. NP_631896.1. NP_998783.1.
UniGene	Hs.117715
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P78524.
PTM databases
PhosphoSite	P78524.
Genome annotation databases
Ensembl	ENSG00000166444. Homo sapiens. [Contig view]
GeneID	6764.
KEGG	hsa:6764.
Organism-specific databases
HGNC	HGNC:11350. ST5.
MIM	140750. gene.
PharmGKB	PA26505.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	P78524.
Gene expression databases
ArrayExpress	P78524.
CleanEx	HS_ST5.
GermOnline	ENSG00000166444. Homo sapiens.
Family and domain databases
InterPro	IPR005112. dDENN. IPR001194. DENN. IPR005113. uDENN. [Graphical view]
Pfam	PF03455. dDENN. 1 hit. PF02141. DENN. 1 hit. PF03456. uDENN. 1 hit. [Graphical view]
SMART	SM00801. dDENN. 1 hit. SM00799. DENN. 1 hit. SM00800. uDENN. 1 hit. [Graphical view]
PROSITE	PS50947. DDENN. 1 hit. PS50211. DENN. 1 hit. PS50946. UDENN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	26398.
SOURCE	Search...

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Entry information

Entry name

ST5_HUMAN

Accession

Primary (citable) accession number: P78524
Secondary accession number(s): P78523

Q9BQQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	November 25, 2008
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents