P78524 (ST5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Suppression of tumorigenicity 5 protein

Alternative name(s):
DENN domain-containing protein 2B
HeLa tumor suppression 1

Gene names

Name:	ST5
Synonyms:	DENND2B, HTS1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1137 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in cytoskeletal organization and tumorgenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth. Ref.8 Ref.9
Subunit structure	Isoform 1 interacts with the SH3 domain of ABL1. Ref.8
Tissue specificity	Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level). Ref.9
Sequence similarities	Contains 1 dDENN domain. Contains 1 DENN domain. Contains 1 uDENN domain.
Sequence caution	The sequence AAC50925.1 differs from that shown. Reason: Frameshift at positions 159 and 169.

Ontologies

Keywords
Coding sequence diversity	Alternative promoter usage Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of ERK1 and ERK2 cascade Inferred from direct assay Ref.8. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]

Isoform 1 (identifier: P78524-1)

Also known as: p126;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by alternative promoter usage.

Isoform 2 (identifier: P78524-2)

Also known as: p82;

The sequence of this isoform differs from the canonical sequence as follows:
     28-447: Missing.

Note: Produced by alternative splicing of isoform 1.

Isoform 3 (identifier: P78524-3)

Also known as: p70;

The sequence of this isoform differs from the canonical sequence as follows:
     1-528: Missing.

Note: Produced by alternative promoter usage.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1137	1137	Suppression of tumorigenicity 5 protein		PRO_0000247448
Regions
	Domain	709 – 784	76	UDENN
	Domain	791 – 975	185	DENN
	Domain	1022 – 1089	68	dDENN
	Region	401 – 447	47	Interaction with ABL1
	Compositional bias	33 – 93	61	Pro-rich
	Compositional bias	350 – 434	85	Pro-rich
Amino acid modifications
	Modified residue	622	1	Phosphoserine Ref.11
	Modified residue	1011	1	Phosphoserine Ref.10
	Modified residue	1013	1	Phosphoserine Ref.10
	Modified residue	1015	1	Phosphoserine Ref.10
Natural variations
	Alternative sequence	1 – 528	528	Missing in isoform 3.		VSP_019987
	Alternative sequence	28 – 447	420	Missing in isoform 2.		VSP_019988
	Natural variant	316	1	K → N. Ref.6 Corresponds to variant rs3794153 [ dbSNP | Ensembl ].		VAR_027101
	Natural variant	399	1	D → E. Ref.1 Ref.2 Corresponds to variant rs3812762 [ dbSNP | Ensembl ].		VAR_027102
	Natural variant	620	1	I → T. Ref.6 Corresponds to variant rs17853683 [ dbSNP | Ensembl ].		VAR_027103
	Natural variant	657	1	S → F. Corresponds to variant rs11042047 [ dbSNP | Ensembl ].		VAR_030642
	Natural variant	774	1	S → G. Ref.6 Corresponds to variant rs17853682 [ dbSNP | Ensembl ].		VAR_027104
Experimental info
	Sequence conflict	113	1	A → T in AAC50925. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (p126) [UniParc]. Last modified January 11, 2011. Version 3. Checksum: 96ACD16E0602479E	FASTA	1,137	126,485
	10 20 30 40 50 60 MTMTANKNSS ITHGAGGTKA PRGTLSRSQS VSPPPVLSPP RSPIYPLSDS ETSACRYPSH 70 80 90 100 110 120 SSSRVLLKDR HPPAPSPQNP QDPSPDTSPP TCPFKTASFG YLDRSPSACK RDAQKESVQG 130 140 150 160 170 180 AAQDVAGVAA CLPLAQSTPF PGPAAGPRGV LLTRTGTRAH SLGIREKISA WEGRREASPR 190 200 210 220 230 240 MSMCGEKREG SGSEWAASEG CPSLGCPSVV PSPCSSEKTF DFKGLRRMSR TFSECSYPET 250 260 270 280 290 300 EEEGEALPVR DSFYRLEKRL GRSEPSAFLR GHGSRKESSA VLSRIQKIEQ VLKEQPGRGL 310 320 330 340 350 360 PQLPSSCYSV DRGKRKTGTL GSLEEPAGGA SVSAGSRAVG VAGVAGEAGP PPEREGSGST 370 380 390 400 410 420 KPGTPGNSPS SQRLPSKSSL DPAVNPVPKP KRTFEYEADK NPKSKPSNGL PPSPTPAAPP 430 440 450 460 470 480 PLPSTPAPPV TRRPKKDMRG HRKSQSRKSF EFEDASSLQS LYPSSPTENG TENQPKFGSK 490 500 510 520 530 540 STLEENAYED IVGDLPKENP YEDVDLKSRR AGRKSQQLSE NSLDSLHRMW SPQDRKYNSP 550 560 570 580 590 600 PTQLSLKPNS QSLRSGNWSE RKSHRLPRLP KRHSHDDMLL LAQLSLPSSP SSLNEDSLST 610 620 630 640 650 660 TSELLSSRRA RRIPKLVQRI NSIYNAKRGK KRLKKLSMSS IETASLRDEN SESESDSDDR 670 680 690 700 710 720 FKAHTQRLVH IQSMLKRAPS YRTLELELLE WQERELFEYF VVVSLKKKPS RNTYLPEVSY 730 740 750 760 770 780 QFPKLDRPTK QMREAEERLK AIPQFCFPDA KDWLPVSEYS SETFSFMLTG EDGSRRFGYC 790 800 810 820 830 840 RRLLPSGKGP RLPEVYCVIS RLGCFGLFSK VLDEVERRRG ISAALVYPFM RSLMESPFPA 850 860 870 880 890 900 PGKTIKVKTF LPGAGNEVLE LRRPMDSRLE HVDFECLFTC LSVRQLIRIF ASLLLERRVI 910 920 930 940 950 960 FVADKLSTLS SCSHAVVALL YPFSWQHTFI PVLPASMIDI VCCPTPFLVG LLSSSLPKLK 970 980 990 1000 1010 1020 ELPVEEALMV NLGSDRFIRQ MDDEDTLLPR KLQAALEQAL ERKNELISQD SDSDSDDECN 1030 1040 1050 1060 1070 1080 TLNGLVSEVF IRFFVETVGH YSLFLTQSEK GERAFQREAF RKSVASKSIR RFLEVFMESQ 1090 1100 1110 1120 1130 MFAGFIQDRE LRKCRAKGLF EQRVEQYLEE LPDTEQSGMN KFLRGLGNKM KFLHKKN
	Isoform 2 (p82) [UniParc]. Checksum: 0329586AEE3E05BE	FASTA	717	82,079
	10 20 30 40 50 60 MTMTANKNSS ITHGAGGTKA PRGTLSRKSF EFEDASSLQS LYPSSPTENG TENQPKFGSK 70 80 90 100 110 120 STLEENAYED IVGDLPKENP YEDVDLKSRR AGRKSQQLSE NSLDSLHRMW SPQDRKYNSP 130 140 150 160 170 180 PTQLSLKPNS QSLRSGNWSE RKSHRLPRLP KRHSHDDMLL LAQLSLPSSP SSLNEDSLST 190 200 210 220 230 240 TSELLSSRRA RRIPKLVQRI NSIYNAKRGK KRLKKLSMSS IETASLRDEN SESESDSDDR 250 260 270 280 290 300 FKAHTQRLVH IQSMLKRAPS YRTLELELLE WQERELFEYF VVVSLKKKPS RNTYLPEVSY 310 320 330 340 350 360 QFPKLDRPTK QMREAEERLK AIPQFCFPDA KDWLPVSEYS SETFSFMLTG EDGSRRFGYC 370 380 390 400 410 420 RRLLPSGKGP RLPEVYCVIS RLGCFGLFSK VLDEVERRRG ISAALVYPFM RSLMESPFPA 430 440 450 460 470 480 PGKTIKVKTF LPGAGNEVLE LRRPMDSRLE HVDFECLFTC LSVRQLIRIF ASLLLERRVI 490 500 510 520 530 540 FVADKLSTLS SCSHAVVALL YPFSWQHTFI PVLPASMIDI VCCPTPFLVG LLSSSLPKLK 550 560 570 580 590 600 ELPVEEALMV NLGSDRFIRQ MDDEDTLLPR KLQAALEQAL ERKNELISQD SDSDSDDECN 610 620 630 640 650 660 TLNGLVSEVF IRFFVETVGH YSLFLTQSEK GERAFQREAF RKSVASKSIR RFLEVFMESQ 670 680 690 700 710 MFAGFIQDRE LRKCRAKGLF EQRVEQYLEE LPDTEQSGMN KFLRGLGNKM KFLHKKN
	Isoform 3 (p70) [UniParc]. Checksum: 59F8FB669B1058E5	FASTA	609	70,245
	10 20 30 40 50 60 MWSPQDRKYN SPPTQLSLKP NSQSLRSGNW SERKSHRLPR LPKRHSHDDM LLLAQLSLPS 70 80 90 100 110 120 SPSSLNEDSL STTSELLSSR RARRIPKLVQ RINSIYNAKR GKKRLKKLSM SSIETASLRD 130 140 150 160 170 180 ENSESESDSD DRFKAHTQRL VHIQSMLKRA PSYRTLELEL LEWQERELFE YFVVVSLKKK 190 200 210 220 230 240 PSRNTYLPEV SYQFPKLDRP TKQMREAEER LKAIPQFCFP DAKDWLPVSE YSSETFSFML 250 260 270 280 290 300 TGEDGSRRFG YCRRLLPSGK GPRLPEVYCV ISRLGCFGLF SKVLDEVERR RGISAALVYP 310 320 330 340 350 360 FMRSLMESPF PAPGKTIKVK TFLPGAGNEV LELRRPMDSR LEHVDFECLF TCLSVRQLIR 370 380 390 400 410 420 IFASLLLERR VIFVADKLST LSSCSHAVVA LLYPFSWQHT FIPVLPASMI DIVCCPTPFL 430 440 450 460 470 480 VGLLSSSLPK LKELPVEEAL MVNLGSDRFI RQMDDEDTLL PRKLQAALEQ ALERKNELIS 490 500 510 520 530 540 QDSDSDSDDE CNTLNGLVSE VFIRFFVETV GHYSLFLTQS EKGERAFQRE AFRKSVASKS 550 560 570 580 590 600 IRRFLEVFME SQMFAGFIQD RELRKCRAKG LFEQRVEQYL EELPDTEQSG MNKFLRGLGN KMKFLHKKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential expression of the human ST5 gene in HeLa-fibroblast hybrid cell lines mediated by YY1: evidence that YY1 plays a part in tumor suppression." Lichy J.H., Majidi M., Elbaum J., Tsai M.M. Nucleic Acids Res. 24:4700-4708(1996) [PubMed: 8972856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), VARIANT GLU-399.
[2]	"Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7." Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A., Zabel B., Hankeln T., Schmidt E.R. Cytogenet. Cell Genet. 93:284-290(2001) [PubMed: 11528127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-399.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Brain and Spleen.
[4]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ASN-316; THR-620 AND GLY-774. Tissue: Testis.
[7]	"Identification of a human chromosome 11 gene which is differentially regulated in tumorigenic and nontumorigenic somatic cell hybrids of HeLa cells." Lichy J.H., Modi W.S., Seuanez H.N., Howley P.M. Cell Growth Differ. 3:541-548(1992) [PubMed: 1390339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 493-1137.
[8]	"Activation of extracellular signal-regulated kinase 2 by a novel Abl-binding protein, ST5." Majidi M., Hubbs A.E., Lichy J.H. J. Biol. Chem. 273:16608-16614(1998) [PubMed: 9632734] [Abstract] Cited for: FUNCTION, INTERACTION WITH ABL1.
[9]	"Expression of an isoform of the novel signal transduction protein ST5 is linked to cell morphology." Hubbs A.E., Majidi M., Lichy J.H. Oncogene 18:2519-2525(1999) [PubMed: 10229203] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1011; SER-1013 AND SER-1015, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-622, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15131 mRNA. Translation: AAC50925.1. Frameshift. U15779 mRNA. Translation: AAB97097.1. U15780 mRNA. Translation: AAC50926.1. AJ400879 Genomic DNA. Translation: CAC35387.1. AK127763 mRNA. Translation: BAG54568.1. AK312758 mRNA. Translation: BAG35624.1. AC026894 Genomic DNA. No translation available. AC091053 Genomic DNA. No translation available. CH471064 Genomic DNA. Translation: EAW68618.1. BC036655 mRNA. Translation: AAH36655.1. S45936 mRNA. Translation: AAB23647.1.
IPI	IPI00162743. IPI00298518. IPI00783821.
PIR	A49013.
RefSeq	NP_005409.3. NM_005418.3. NP_631896.1. NM_139157.2. NP_998783.1. NM_213618.1.
UniGene	Hs.117715.
3D structure databases
ProteinModelPortal	P78524.
ModBase	Search...
Protein-protein interaction databases
IntAct	P78524. 3 interactions.
MINT	MINT-1184327.
STRING	P78524.
PTM databases
PhosphoSite	P78524.
Polymorphism databases
DMDM	110816432.
Proteomic databases
PRIDE	P78524.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000313726; ENSP00000319678; ENSG00000166444. ENST00000357665; ENSP00000350294; ENSG00000166444. ENST00000396665; ENSP00000379900; ENSG00000166444.
GeneID	6764.
KEGG	hsa:6764.
UCSC	uc001mgt.1. human.
Organism-specific databases
CTD	6764.
GeneCards	GC11M008671.
HGNC	HGNC:11350. ST5.
MIM	140750. gene.
neXtProt	NX_P78524.
PharmGKB	PA36172.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15669.
HOVERGEN	HBG079980.
InParanoid	P78524.
OMA	ACRYPSH.
OrthoDB	EOG4CRKZC.
PhylomeDB	P78524.
Gene expression databases
ArrayExpress	P78524.
Bgee	P78524.
CleanEx	HS_ST5.
Genevestigator	P78524.
GermOnline	ENSG00000166444. Homo sapiens.
Family and domain databases
InterPro	IPR005112. dDENN. IPR001194. DENN. IPR005113. uDENN. [Graphical view]
Pfam	PF03455. dDENN. 1 hit. PF02141. DENN. 1 hit. PF03456. uDENN. 1 hit. [Graphical view]
SMART	SM00801. dDENN. 1 hit. SM00799. DENN. 1 hit. SM00800. uDENN. 1 hit. [Graphical view]
PROSITE	PS50947. DDENN. 1 hit. PS50211. DENN. 1 hit. PS50946. UDENN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	26398.
SOURCE	Search...

Entry information

Entry name

ST5_HUMAN

Accession

Primary (citable) accession number: P78524
Secondary accession number(s): B2R6X7 , B3KXQ6, P78523, Q16492, Q7KYY2, Q7KZ12, Q8NE12, Q9BQQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	January 11, 2011
Last modified:	January 25, 2012
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.