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P78524 (ST5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Suppression of tumorigenicity 5 protein
Alternative name(s):
DENN domain-containing protein 2B
HeLa tumor suppression 1
Gene names
Name:ST5
Synonyms:DENND2B, HTS1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1137 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May be involved in cytoskeletal organization and tumorogenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth. Ref.8 Ref.9 Ref.11

Subunit structure

Isoform 1 interacts with the SH3 domain of ABL1. Ref.8

Tissue specificity

Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level). Ref.9

Sequence similarities

Contains 1 dDENN domain.

Contains 1 DENN domain.

Contains 1 uDENN domain.

Sequence caution

The sequence AAC50925.1 differs from that shown. Reason: Frameshift at positions 159 and 169.

Alternative products

This entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]
Isoform 1 (identifier: P78524-1)

Also known as: p126;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by alternative promoter usage.
Isoform 2 (identifier: P78524-2)

Also known as: p82;

The sequence of this isoform differs from the canonical sequence as follows:
     28-447: Missing.
Note: Produced by alternative splicing of isoform 1.
Isoform 3 (identifier: P78524-3)

Also known as: p70;

The sequence of this isoform differs from the canonical sequence as follows:
     1-528: Missing.
Note: Produced by alternative promoter usage.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11371137Suppression of tumorigenicity 5 protein
PRO_0000247448

Regions

Domain709 – 78476UDENN
Domain791 – 975185DENN
Domain1022 – 108968dDENN
Region401 – 44747Interaction with ABL1
Compositional bias33 – 9361Pro-rich
Compositional bias350 – 43485Pro-rich

Amino acid modifications

Modified residue6221Phosphoserine Ref.10

Natural variations

Alternative sequence1 – 528528Missing in isoform 3.
VSP_019987
Alternative sequence28 – 447420Missing in isoform 2.
VSP_019988
Natural variant3161K → N. Ref.6
Corresponds to variant rs3794153 [ dbSNP | Ensembl ].
VAR_027101
Natural variant3991D → E. Ref.1 Ref.2
Corresponds to variant rs3812762 [ dbSNP | Ensembl ].
VAR_027102
Natural variant6201I → T. Ref.6
Corresponds to variant rs17853683 [ dbSNP | Ensembl ].
VAR_027103
Natural variant6571S → F.
Corresponds to variant rs11042047 [ dbSNP | Ensembl ].
VAR_030642
Natural variant7741S → G. Ref.6
Corresponds to variant rs17853682 [ dbSNP | Ensembl ].
VAR_027104

Experimental info

Sequence conflict1131A → T in AAC50925. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (p126) [UniParc].

Last modified January 11, 2011. Version 3.
Checksum: 96ACD16E0602479E

FASTA1,137126,485
        10         20         30         40         50         60 
MTMTANKNSS ITHGAGGTKA PRGTLSRSQS VSPPPVLSPP RSPIYPLSDS ETSACRYPSH 

        70         80         90        100        110        120 
SSSRVLLKDR HPPAPSPQNP QDPSPDTSPP TCPFKTASFG YLDRSPSACK RDAQKESVQG 

       130        140        150        160        170        180 
AAQDVAGVAA CLPLAQSTPF PGPAAGPRGV LLTRTGTRAH SLGIREKISA WEGRREASPR 

       190        200        210        220        230        240 
MSMCGEKREG SGSEWAASEG CPSLGCPSVV PSPCSSEKTF DFKGLRRMSR TFSECSYPET 

       250        260        270        280        290        300 
EEEGEALPVR DSFYRLEKRL GRSEPSAFLR GHGSRKESSA VLSRIQKIEQ VLKEQPGRGL 

       310        320        330        340        350        360 
PQLPSSCYSV DRGKRKTGTL GSLEEPAGGA SVSAGSRAVG VAGVAGEAGP PPEREGSGST 

       370        380        390        400        410        420 
KPGTPGNSPS SQRLPSKSSL DPAVNPVPKP KRTFEYEADK NPKSKPSNGL PPSPTPAAPP 

       430        440        450        460        470        480 
PLPSTPAPPV TRRPKKDMRG HRKSQSRKSF EFEDASSLQS LYPSSPTENG TENQPKFGSK 

       490        500        510        520        530        540 
STLEENAYED IVGDLPKENP YEDVDLKSRR AGRKSQQLSE NSLDSLHRMW SPQDRKYNSP 

       550        560        570        580        590        600 
PTQLSLKPNS QSLRSGNWSE RKSHRLPRLP KRHSHDDMLL LAQLSLPSSP SSLNEDSLST 

       610        620        630        640        650        660 
TSELLSSRRA RRIPKLVQRI NSIYNAKRGK KRLKKLSMSS IETASLRDEN SESESDSDDR 

       670        680        690        700        710        720 
FKAHTQRLVH IQSMLKRAPS YRTLELELLE WQERELFEYF VVVSLKKKPS RNTYLPEVSY 

       730        740        750        760        770        780 
QFPKLDRPTK QMREAEERLK AIPQFCFPDA KDWLPVSEYS SETFSFMLTG EDGSRRFGYC 

       790        800        810        820        830        840 
RRLLPSGKGP RLPEVYCVIS RLGCFGLFSK VLDEVERRRG ISAALVYPFM RSLMESPFPA 

       850        860        870        880        890        900 
PGKTIKVKTF LPGAGNEVLE LRRPMDSRLE HVDFECLFTC LSVRQLIRIF ASLLLERRVI 

       910        920        930        940        950        960 
FVADKLSTLS SCSHAVVALL YPFSWQHTFI PVLPASMIDI VCCPTPFLVG LLSSSLPKLK 

       970        980        990       1000       1010       1020 
ELPVEEALMV NLGSDRFIRQ MDDEDTLLPR KLQAALEQAL ERKNELISQD SDSDSDDECN 

      1030       1040       1050       1060       1070       1080 
TLNGLVSEVF IRFFVETVGH YSLFLTQSEK GERAFQREAF RKSVASKSIR RFLEVFMESQ 

      1090       1100       1110       1120       1130 
MFAGFIQDRE LRKCRAKGLF EQRVEQYLEE LPDTEQSGMN KFLRGLGNKM KFLHKKN 

« Hide

Isoform 2 (p82) [UniParc].

Checksum: 0329586AEE3E05BE
Show »

FASTA71782,079
Isoform 3 (p70) [UniParc].

Checksum: 59F8FB669B1058E5
Show »

FASTA60970,245

References

« Hide 'large scale' references
[1]"Differential expression of the human ST5 gene in HeLa-fibroblast hybrid cell lines mediated by YY1: evidence that YY1 plays a part in tumor suppression."
Lichy J.H., Majidi M., Elbaum J., Tsai M.M.
Nucleic Acids Res. 24:4700-4708(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), VARIANT GLU-399.
[2]"Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7."
Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A., Zabel B., Hankeln T., Schmidt E.R.
Cytogenet. Cell Genet. 93:284-290(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-399.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Brain and Spleen.
[4]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ASN-316; THR-620 AND GLY-774.
Tissue: Testis.
[7]"Identification of a human chromosome 11 gene which is differentially regulated in tumorigenic and nontumorigenic somatic cell hybrids of HeLa cells."
Lichy J.H., Modi W.S., Seuanez H.N., Howley P.M.
Cell Growth Differ. 3:541-548(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 493-1137.
[8]"Activation of extracellular signal-regulated kinase 2 by a novel Abl-binding protein, ST5."
Majidi M., Hubbs A.E., Lichy J.H.
J. Biol. Chem. 273:16608-16614(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ABL1.
[9]"Expression of an isoform of the novel signal transduction protein ST5 is linked to cell morphology."
Hubbs A.E., Majidi M., Lichy J.H.
Oncogene 18:2519-2525(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-622, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors."
Yoshimura S., Gerondopoulos A., Linford A., Rigden D.J., Barr F.A.
J. Cell Biol. 191:367-381(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS GUANYL-NUCLEOTIDE EXCHANGE FACTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15131 mRNA. Translation: AAC50925.1. Frameshift.
U15779 mRNA. Translation: AAB97097.1.
U15780 mRNA. Translation: AAC50926.1.
AJ400879 Genomic DNA. Translation: CAC35387.1.
AK127763 mRNA. Translation: BAG54568.1.
AK312758 mRNA. Translation: BAG35624.1.
AC026894 Genomic DNA. No translation available.
AC091053 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68618.1.
BC036655 mRNA. Translation: AAH36655.1.
S45936 mRNA. Translation: AAB23647.1.
CCDSCCDS7791.1. [P78524-1]
CCDS7792.1. [P78524-2]
PIRA49013.
RefSeqNP_005409.3. NM_005418.3. [P78524-1]
NP_631896.1. NM_139157.2. [P78524-2]
NP_998783.1. NM_213618.1. [P78524-1]
XP_005253136.1. XM_005253079.1. [P78524-1]
XP_005253137.1. XM_005253080.1. [P78524-1]
XP_005253140.1. XM_005253083.1. [P78524-2]
XP_005253141.1. XM_005253084.1. [P78524-2]
XP_006718347.1. XM_006718284.1. [P78524-1]
XP_006718348.1. XM_006718285.1. [P78524-1]
UniGeneHs.117715.

3D structure databases

ProteinModelPortalP78524.
SMRP78524. Positions 736-1089.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112642. 5 interactions.
IntActP78524. 5 interactions.
MINTMINT-1184327.

PTM databases

PhosphoSiteP78524.

Polymorphism databases

DMDM317373507.

Proteomic databases

MaxQBP78524.
PaxDbP78524.
PRIDEP78524.

Protocols and materials databases

DNASU6764.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000313726; ENSP00000319678; ENSG00000166444. [P78524-1]
ENST00000357665; ENSP00000350294; ENSG00000166444. [P78524-1]
ENST00000526757; ENSP00000435097; ENSG00000166444. [P78524-2]
ENST00000530438; ENSP00000436802; ENSG00000166444. [P78524-2]
ENST00000530991; ENSP00000432887; ENSG00000166444. [P78524-3]
ENST00000534127; ENSP00000433528; ENSG00000166444. [P78524-1]
GeneID6764.
KEGGhsa:6764.
UCSCuc001mgt.3. human. [P78524-1]
uc001mgu.3. human. [P78524-2]

Organism-specific databases

CTD6764.
GeneCardsGC11M008714.
H-InvDBHIX0026155.
HGNCHGNC:11350. ST5.
HPAHPA046796.
MIM140750. gene.
neXtProtNX_P78524.
PharmGKBPA36172.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG247048.
HOVERGENHBG079980.
InParanoidP78524.
OMADRKYNNP.
OrthoDBEOG79GT6H.
PhylomeDBP78524.
TreeFamTF320336.

Gene expression databases

ArrayExpressP78524.
BgeeP78524.
CleanExHS_ST5.
GenevestigatorP78524.

Family and domain databases

InterProIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
PROSITEPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSST5. human.
GeneWikiST5_(gene).
GenomeRNAi6764.
NextBio26398.
PROP78524.
SOURCESearch...

Entry information

Entry nameST5_HUMAN
AccessionPrimary (citable) accession number: P78524
Secondary accession number(s): B2R6X7 expand/collapse secondary AC list , B3KXQ6, P78523, Q16492, Q7KYY2, Q7KZ12, Q8NE12, Q9BQQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM