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P78426 (NKX61_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein Nkx-6.1
Alternative name(s):
Homeobox protein NK-6 homolog A
Gene names
Name:NKX6-1
Synonyms:NKX6A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals By similarity.

Subcellular location

Nucleus Probable.

Tissue specificity

Pancreatic beta cells.

Domain

The C-terminal domain contributes to sequence-specific DNA-binding By similarity.

Sequence similarities

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
Repressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell proliferation

Inferred from electronic annotation. Source: Ensembl

cellular response to cytokine stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to peptide hormone stimulus

Inferred from electronic annotation. Source: Ensembl

detection of glucose

Inferred from sequence or structural similarity. Source: BHF-UCL

endocrine pancreas development

Traceable author statement. Source: Reactome

negative regulation of glial cell differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: BHF-UCL

oligodendrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

organ morphogenesis

Traceable author statement PubMed 10567713. Source: ProtInc

pancreas development

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of glial cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of insulin secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of neuron differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of type B pancreatic cell development

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification

Inferred from electronic annotation. Source: Ensembl

regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to nicotine

Inferred from electronic annotation. Source: Ensembl

smoothened signaling pathway

Inferred from electronic annotation. Source: Ensembl

transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

type B pancreatic cell maturation

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentextracellular vesicular exosome

Inferred from direct assay PubMed 19056867. Source: UniProt

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionchromatin binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

transcription regulatory region sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367Homeobox protein Nkx-6.1
PRO_0000048951

Regions

DNA binding236 – 29560Homeobox
Region102 – 268167Repressor domain By similarity
Region306 – 36762Involved in DNA-binding By similarity
Compositional bias49 – 6113Poly-Ser
Compositional bias119 – 13517Poly-Ser
Compositional bias136 – 15015Poly-Ala
Compositional bias168 – 1736Poly-Pro
Compositional bias351 – 3555Poly-Gly

Experimental info

Sequence conflict1001D → N in AAD11962. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P78426 [UniParc].

Last modified February 9, 2010. Version 2.
Checksum: 5C78F06C3CAE6486

FASTA36737,849
        10         20         30         40         50         60 
MLAVGAMEGT RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPAGPPSS SSSSSSSSSP 

        70         80         90        100        110        120 
SPPLGTHNPG GLKPPATGGL SSLGSPPQQL SAATPHGIND ILSRPSMPVA SGAALPSASP 

       130        140        150        160        170        180 
SGSSSSSSSS ASASSASAAA AAAAAAAAAA SSPAGLLAGL PRFSSLSPPP PPPGLYFSPS 

       190        200        210        220        230        240 
AAAVAAVGRY PKPLAELPGR TPIFWPGVMQ SPPWRDARLA CTPHQGSILL DKDGKRKHTR 

       250        260        270        280        290        300 
PTFSGQQIFA LEKTFEQTKY LAGPERARLA YSLGMTESQV KVWFQNRRTK WRKKHAAEMA 

       310        320        330        340        350        360 
TAKKKQDSET ERLKGASENE EEDDDYNKPL DPNSDDEKIT QLLKKHKSSS GGGGGLLLHA 


SEPESSS 

« Hide

References

« Hide 'large scale' references
[1]"Isolation, characterization, and chromosomal mapping of the human Nkx6.1 gene (NKX6A), a new pancreatic islet homeobox gene."
Inoue H., Rudnick A., German M.S., Veile R., Donis-Keller H., Permutt M.A.
Genomics 40:367-370(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Pancreatic islet.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U66799, U66797, U66798 Genomic DNA. Translation: AAD11962.1.
AC096766 Genomic DNA. No translation available.
CCDSCCDS3607.1.
RefSeqNP_006159.2. NM_006168.2.
XP_006714293.1. XM_006714230.1.
UniGeneHs.546270.

3D structure databases

ProteinModelPortalP78426.
SMRP78426. Positions 243-293.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000295886.

PTM databases

PhosphoSiteP78426.

Polymorphism databases

DMDM288558819.

Proteomic databases

PaxDbP78426.
PRIDEP78426.

Protocols and materials databases

DNASU4825.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000295886; ENSP00000295886; ENSG00000163623.
GeneID4825.
KEGGhsa:4825.
UCSCuc003hpa.1. human.

Organism-specific databases

CTD4825.
GeneCardsGC04M085413.
HGNCHGNC:7839. NKX6-1.
HPAHPA036774.
MIM602563. gene.
neXtProtNX_P78426.
PharmGKBPA31646.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG265304.
HOGENOMHOG000060127.
HOVERGENHBG070987.
InParanoidP78426.
KOK08030.
OMATPLYPAY.
OrthoDBEOG7Z95QB.
PhylomeDBP78426.
TreeFamTF327063.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.

Gene expression databases

ArrayExpressP78426.
BgeeP78426.
CleanExHS_NKX6-1.
GenevestigatorP78426.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiNKX6-1.
GenomeRNAi4825.
NextBio18584.
PROP78426.
SOURCESearch...

Entry information

Entry nameNKX61_HUMAN
AccessionPrimary (citable) accession number: P78426
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 9, 2010
Last modified: July 9, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM