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Protein

Homeobox protein Nkx-6.1

Gene

NKX6-1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi236 – 295HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163623-MONOMER.
ReactomeiR-HSA-210745. Regulation of gene expression in beta cells.
R-HSA-210747. Regulation of gene expression in early pancreatic precursor cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Nkx-6.1
Alternative name(s):
Homeobox protein NK-6 homolog A
Gene namesi
Name:NKX6-1
Synonyms:NKX6A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:7839. NKX6-1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4825.
OpenTargetsiENSG00000163623.
PharmGKBiPA31646.

Polymorphism and mutation databases

BioMutaiNKX6-1.
DMDMi288558819.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000489511 – 367Homeobox protein Nkx-6.1Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei189Asymmetric dimethylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP78426.
PeptideAtlasiP78426.
PRIDEiP78426.

PTM databases

iPTMnetiP78426.
PhosphoSitePlusiP78426.

Expressioni

Tissue specificityi

Pancreatic beta cells.

Gene expression databases

BgeeiENSG00000163623.
CleanExiHS_NKX6-1.
ExpressionAtlasiP78426. baseline and differential.
GenevisibleiP78426. HS.

Organism-specific databases

HPAiCAB070169.
HPA036774.

Interactioni

Protein-protein interaction databases

BioGridi110889. 1 interactor.
IntActiP78426. 1 interactor.
STRINGi9606.ENSP00000295886.

Structurei

3D structure databases

ProteinModelPortaliP78426.
SMRiP78426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 268Repressor domainBy similarityAdd BLAST167
Regioni306 – 367Involved in DNA-bindingBy similarityAdd BLAST62

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi49 – 61Poly-SerAdd BLAST13
Compositional biasi119 – 135Poly-SerAdd BLAST17
Compositional biasi136 – 150Poly-AlaAdd BLAST15
Compositional biasi168 – 173Poly-Pro6
Compositional biasi351 – 355Poly-Gly5

Domaini

The C-terminal domain contributes to sequence-specific DNA-binding.By similarity

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0847. Eukaryota.
ENOG410XT1X. LUCA.
GeneTreeiENSGT00860000133665.
HOGENOMiHOG000060127.
HOVERGENiHBG070987.
InParanoidiP78426.
KOiK08030.
OMAiVGAMEGP.
OrthoDBiEOG091G142D.
PhylomeDBiP78426.
TreeFamiTF327063.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
IPR033630. NKX-6.1.
[Graphical view]
PANTHERiPTHR24340:SF31. PTHR24340:SF31. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78426-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAVGAMEGT RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPAGPPSS
60 70 80 90 100
SSSSSSSSSP SPPLGTHNPG GLKPPATGGL SSLGSPPQQL SAATPHGIND
110 120 130 140 150
ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASASSASAAA AAAAAAAAAA
160 170 180 190 200
SSPAGLLAGL PRFSSLSPPP PPPGLYFSPS AAAVAAVGRY PKPLAELPGR
210 220 230 240 250
TPIFWPGVMQ SPPWRDARLA CTPHQGSILL DKDGKRKHTR PTFSGQQIFA
260 270 280 290 300
LEKTFEQTKY LAGPERARLA YSLGMTESQV KVWFQNRRTK WRKKHAAEMA
310 320 330 340 350
TAKKKQDSET ERLKGASENE EEDDDYNKPL DPNSDDEKIT QLLKKHKSSS
360
GGGGGLLLHA SEPESSS
Length:367
Mass (Da):37,849
Last modified:February 9, 2010 - v2
Checksum:i5C78F06C3CAE6486
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100D → N in AAD11962 (PubMed:9119408).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66799, U66797, U66798 Genomic DNA. Translation: AAD11962.1.
AC096766 Genomic DNA. No translation available.
CCDSiCCDS3607.1.
RefSeqiNP_006159.2. NM_006168.2.
UniGeneiHs.546270.

Genome annotation databases

EnsembliENST00000295886; ENSP00000295886; ENSG00000163623.
GeneIDi4825.
KEGGihsa:4825.
UCSCiuc003hpa.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66799, U66797, U66798 Genomic DNA. Translation: AAD11962.1.
AC096766 Genomic DNA. No translation available.
CCDSiCCDS3607.1.
RefSeqiNP_006159.2. NM_006168.2.
UniGeneiHs.546270.

3D structure databases

ProteinModelPortaliP78426.
SMRiP78426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110889. 1 interactor.
IntActiP78426. 1 interactor.
STRINGi9606.ENSP00000295886.

PTM databases

iPTMnetiP78426.
PhosphoSitePlusiP78426.

Polymorphism and mutation databases

BioMutaiNKX6-1.
DMDMi288558819.

Proteomic databases

PaxDbiP78426.
PeptideAtlasiP78426.
PRIDEiP78426.

Protocols and materials databases

DNASUi4825.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295886; ENSP00000295886; ENSG00000163623.
GeneIDi4825.
KEGGihsa:4825.
UCSCiuc003hpa.2. human.

Organism-specific databases

CTDi4825.
DisGeNETi4825.
GeneCardsiNKX6-1.
HGNCiHGNC:7839. NKX6-1.
HPAiCAB070169.
HPA036774.
MIMi602563. gene.
neXtProtiNX_P78426.
OpenTargetsiENSG00000163623.
PharmGKBiPA31646.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0847. Eukaryota.
ENOG410XT1X. LUCA.
GeneTreeiENSGT00860000133665.
HOGENOMiHOG000060127.
HOVERGENiHBG070987.
InParanoidiP78426.
KOiK08030.
OMAiVGAMEGP.
OrthoDBiEOG091G142D.
PhylomeDBiP78426.
TreeFamiTF327063.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163623-MONOMER.
ReactomeiR-HSA-210745. Regulation of gene expression in beta cells.
R-HSA-210747. Regulation of gene expression in early pancreatic precursor cells.

Miscellaneous databases

GeneWikiiNKX6-1.
GenomeRNAii4825.
PROiP78426.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163623.
CleanExiHS_NKX6-1.
ExpressionAtlasiP78426. baseline and differential.
GenevisibleiP78426. HS.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
IPR033630. NKX-6.1.
[Graphical view]
PANTHERiPTHR24340:SF31. PTHR24340:SF31. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNKX61_HUMAN
AccessioniPrimary (citable) accession number: P78426
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 9, 2010
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.