Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot P78423 (X3CL1_HUMAN)

Last modified November 25, 2008. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Fractalkine
Alternative name(s):
    C-X3-C motif chemokine 1
    Neurotactin
    CX3C membrane-anchored chemokine
    Small-inducible cytokine D1
Cleaved into the following chain:
    1- Recommended name:
            Processed fractalkine
Gene names
Name: CX3CL1
Synonyms: A-152E5.2, FKN, NTT, SCYD1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.

Subunit structure

Monomer.

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Processed fractalkine: Secreted.

Tissue specificity

Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.

Induction

By TNF and IL-1 in pulmonary endothelial cells and umbilical vein endothelial cells.

Post-translational modification

A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form By similarity.

Sequence similarities

Belongs to the intercrine delta family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 397373Fractalkine
PRO_0000005252
Chain25 – ?339315Processed fractalkine
PRO_0000296224

Regions

Topological domain25 – 341317Extracellular Potential
Transmembrane342 – 36221 Potential
Topological domain363 – 39735Cytoplasmic Potential
Region25 – 10076Chemokine
Region101 – 341241Mucin-like stalk

Sites

Site339 – 3402Cleavage; to produce soluble form Potential

Amino acid modifications

Glycosylation331N-linked (GlcNAc...) Potential
Disulfide bond32 ↔ 58
Disulfide bond36 ↔ 74

Secondary structure

................ 397
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P78423-1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: BAC71F5080DF9CA6

FASTA39742,203
        10         20         30         40         50         60 
MAPISLSWLL RLATFCHLTV LLAGQHHGVT KCNITCSKMT SKIPVALLIH YQQNQASCGK 

        70         80         90        100        110        120 
RAIILETRQH RLFCADPKEQ WVKDAMQHLD RQAAALTRNG GTFEKQIGEV KPRTTPAAGG 

       130        140        150        160        170        180 
MDESVVLEPE ATGESSSLEP TPSSQEAQRA LGTSPELPTG VTGSSGTRLP PTPKAQDGGP 

       190        200        210        220        230        240 
VGTELFRVPP VSTAATWQSS APHQPGPSLW AEAKTSEAPS TQDPSTQAST ASSPAPEENA 

       250        260        270        280        290        300 
PSEGQRVWGQ GQSPRPENSL EREEMGPVPA HTDAFQDWGP GSMAHVSVVP VSSEGTPSRE 

       310        320        330        340        350        360 
PVASGSWTPK AEEPIHATMD PQRLGVLITP VPDAQAATRR QAVGLLAFLG LLFCLGVAMF 

       370        380        390 
TYQSLQGCPR KMAGEMAEGL RYIPRSCGSN SYVLVPV 

« Hide

References

« Hide 'large scale' references
[1]"A new class of membrane-bound chemokine with a CX3C motif."
Bazan J.F., Bacon K.B., Hardiman G., Wang W., Soo K., Rossi D., Greaves D.R., Zlotnik A., Schall T.J.
Nature 385:640-644(1997) [PubMed: 9024663] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q."
Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D.
Genomics 60:295-308(1999) [PubMed: 10493829] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Solution structure and dynamics of the CX3C chemokine domain of fractalkine and its interaction with an N-terminal fragment of CX3CR1."
Mizoue L.S., Bazan J.F., Johnson E.C., Handel T.M.
Biochemistry 38:1402-1414(1999) [PubMed: 9931005] [Abstract]
Cited for: STRUCTURE BY NMR OF 25-100.
+Additional computationally mapped references.

Web resources

Wikipedia

CX3CL1 entry

Cross-references

Sequence databases

U91835 mRNA. Translation: AAB50014.1.
U84487 mRNA. Translation: AAB49679.1.
AC004382 Genomic DNA. Translation: AAC24307.1.
BC001163 mRNA. Translation: AAH01163.1.
BC016164 mRNA. Translation: AAH16164.1.
RefSeqNP_002987.1.
UniGeneHs.531668

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1B2TNMR-A25-100[»]
1F2LX-ray2.00A/B/C/D25-100[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:5878N.

Proteomic databases

PeptideAtlasP78423.

Genome annotation databases

EnsemblENSG00000006210. Homo sapiens. [Contig view]
GeneID6376.
KEGGhsa:6376.

Organism-specific databases

H-InvDBHIX0013072.
HGNCHGNC:10647. CX3CL1.
MIM601880. gene.
PharmGKBPA35577.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP78423.
HOVERGENP78423.

Gene expression databases

ArrayExpressP78423.
CleanExHS_CX3CL1.
GermOnlineENSG00000006210. Homo sapiens.

Family and domain databases

InterProIPR001811. Chemokine_IL8.
IPR008097. Fractalkine.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
PRINTSPR01721. FRACTALKINE.
SMARTSM00199. SCY. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio24772.
SOURCESearch...

Entry information

Entry nameX3CL1_HUMAN
AccessionPrimary (citable) accession number: P78423
Secondary accession number(s): O00672
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 1, 1997
Last modified: November 25, 2008
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents