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Protein

Glutathione S-transferase omega-1

Gene

GSTO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.5 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.5 Publications
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.2 Publications
Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O.1 Publication

Enzyme regulationi

Monomethylarsonic acid reductase activity is competitively inhibited by 1-chloro 2,4-dinitrobenzene (CDNB) and by deoxycholate.

pH dependencei

Optimum pH is 8.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei32Nucleophile1 Publication1
Binding sitei59Glutathione2 Publications1
Binding sitei72Glutathione; via amide nitrogen and carbonyl oxygen2 Publications1

GO - Molecular functioni

  • glutathione dehydrogenase (ascorbate) activity Source: UniProtKB
  • glutathione transferase activity Source: UniProtKB
  • methylarsonate reductase activity Source: Reactome
  • oxidoreductase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS07564-MONOMER.
ZFISH:HS07564-MONOMER.
BRENDAi1.20.4.2. 2681.
1.8.5.1. 2681.
2.5.1.18. 2681.
ReactomeiR-HSA-156581. Methylation.
R-HSA-156590. Glutathione conjugation.
R-HSA-196836. Vitamin C (ascorbate) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase omega-1 (EC:2.5.1.185 Publications)
Short name:
GSTO-1
Alternative name(s):
Glutathione S-transferase omega 1-1
Short name:
GSTO 1-1
Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.12 Publications)
Monomethylarsonic acid reductase (EC:1.20.4.21 Publication)
Short name:
MMA(V) reductase
S-(Phenacyl)glutathione reductase
Short name:
SPG-R
Gene namesi
Name:GSTO1
Synonyms:GSTTLP28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:13312. GSTO1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi32C → A: Loss of activity. 1 Publication1

Organism-specific databases

DisGeNETi9446.
OpenTargetsiENSG00000148834.
PharmGKBiPA133787054.

Chemistry databases

ChEMBLiCHEMBL3174.
DrugBankiDB00143. Glutathione.
DB00163. Vitamin E.

Polymorphism and mutation databases

BioMutaiGSTO1.
DMDMi6016173.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001858842 – 241Glutathione S-transferase omega-1Add BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei57N6-acetyllysineCombined sources1
Modified residuei129PhosphoserineCombined sources1
Modified residuei143N6-acetyllysineCombined sources1
Modified residuei148N6-acetyllysineCombined sources1
Modified residuei152N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP78417.
PaxDbiP78417.
PeptideAtlasiP78417.
PRIDEiP78417.
TopDownProteomicsiP78417-1. [P78417-1]

2D gel databases

OGPiP78417.
UCD-2DPAGEP78417.

PTM databases

iPTMnetiP78417.
PhosphoSitePlusiP78417.
SwissPalmiP78417.

Expressioni

Tissue specificityi

Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart. Lowest expression in brain, placenta and lung.1 Publication

Gene expression databases

BgeeiENSG00000148834.
CleanExiHS_GSTO1.
ExpressionAtlasiP78417. baseline and differential.
GenevisibleiP78417. HS.

Organism-specific databases

HPAiHPA037603.
HPA037604.

Interactioni

Subunit structurei

Homodimer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSPB1P047922EBI-712083,EBI-352682

Protein-protein interaction databases

BioGridi114836. 35 interactors.
IntActiP78417. 8 interactors.
MINTiMINT-1384709.
STRINGi9606.ENSP00000358727.

Chemistry databases

BindingDBiP78417.

Structurei

Secondary structure

1241
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi24 – 28Combined sources5
Helixi33 – 45Combined sources13
Beta strandi49 – 54Combined sources6
Beta strandi56 – 58Combined sources3
Helixi63 – 66Combined sources4
Beta strandi74 – 76Combined sources3
Beta strandi82 – 85Combined sources4
Helixi86 – 96Combined sources11
Helixi107 – 120Combined sources14
Helixi123 – 131Combined sources9
Helixi136 – 160Combined sources25
Beta strandi162 – 164Combined sources3
Beta strandi167 – 169Combined sources3
Helixi172 – 184Combined sources13
Turni185 – 188Combined sources4
Helixi190 – 192Combined sources3
Beta strandi193 – 195Combined sources3
Helixi197 – 207Combined sources11
Helixi210 – 215Combined sources6
Helixi219 – 229Combined sources11
Turni230 – 232Combined sources3
Helixi236 – 238Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EEMX-ray2.00A1-241[»]
3LFLX-ray2.10A/B/C1-241[»]
3VLNX-ray1.70A1-241[»]
4IS0X-ray1.72A1-241[»]
4YQMX-ray2.38A/B/C1-241[»]
4YQUX-ray1.94A/B1-241[»]
4YQVX-ray2.06A/B/C1-241[»]
ProteinModelPortaliP78417.
SMRiP78417.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78417.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 101GST N-terminalAdd BLAST80
Domaini106 – 230GST C-terminalAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 86Glutathione binding2 Publications2

Sequence similaritiesi

Belongs to the GST superfamily. Omega family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0406. Eukaryota.
ENOG410XSIX. LUCA.
GeneTreeiENSGT00390000005479.
HOGENOMiHOG000006560.
HOVERGENiHBG051853.
InParanoidiP78417.
KOiK00799.
OMAiSTITCEY.
OrthoDBiEOG091G0IEA.
PhylomeDBiP78417.
TreeFamiTF105325.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005442. GST_omega.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01625. GSTRNSFRASEO.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P78417-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGESARSLG KGSAPPGPVP EGSIRIYSMR FCPFAERTRL VLKAKGIRHE
60 70 80 90 100
VININLKNKP EWFFKKNPFG LVPVLENSQG QLIYESAITC EYLDEAYPGK
110 120 130 140 150
KLLPDDPYEK ACQKMILELF SKVPSLVGSF IRSQNKEDYA GLKEEFRKEF
160 170 180 190 200
TKLEEVLTNK KTTFFGGNSI SMIDYLIWPW FERLEAMKLN ECVDHTPKLK
210 220 230 240
LWMAAMKEDP TVSALLTSEK DWQGFLELYL QNSPEACDYG L
Length:241
Mass (Da):27,566
Last modified:January 1, 1998 - v2
Checksum:i9134ABA265F5C87E
GO
Isoform 2 (identifier: P78417-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     123-155: Missing.

Show »
Length:208
Mass (Da):23,709
Checksum:iF00CDE200E2A1738
GO
Isoform 3 (identifier: P78417-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.

Show »
Length:213
Mass (Da):24,796
Checksum:iC3F8EBA348399628
GO

Sequence cautioni

The sequence CAD97673 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06123132C → Y.Corresponds to variant rs45529437dbSNPEnsembl.1
Natural variantiVAR_02926986S → C.Corresponds to variant rs11509436dbSNPEnsembl.1
Natural variantiVAR_016811140A → D in allele GSTO1*C; no effect on protein stability. 3 PublicationsCorresponds to variant rs4925dbSNPEnsembl.1
Natural variantiVAR_016813155Missing in allele GSTO1*B; decreased protein stability. 3 Publications1
Natural variantiVAR_024484208E → K.1 PublicationCorresponds to variant rs11509438dbSNPEnsembl.1
Natural variantiVAR_026583236A → V.1 PublicationCorresponds to variant rs11509439dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0458191 – 28Missing in isoform 3. CuratedAdd BLAST28
Alternative sequenceiVSP_045820123 – 155Missing in isoform 2. CuratedAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90313 mRNA. Translation: AAB70109.1.
AF212303 mRNA. Translation: AAF73376.1.
AY817669 Genomic DNA. Translation: AAV68046.1.
BX537431 mRNA. Translation: CAD97673.1. Different initiation.
AL139341 Genomic DNA. Translation: CAI17224.1.
CH471066 Genomic DNA. Translation: EAW49601.1.
CH471066 Genomic DNA. Translation: EAW49602.1.
CH471066 Genomic DNA. Translation: EAW49603.1.
BC000127 mRNA. Translation: AAH00127.1.
CCDSiCCDS53572.1. [P78417-2]
CCDS53573.1. [P78417-3]
CCDS7555.1. [P78417-1]
RefSeqiNP_001177931.1. NM_001191002.1. [P78417-2]
NP_001177932.1. NM_001191003.1. [P78417-3]
NP_004823.1. NM_004832.2. [P78417-1]
UniGeneiHs.190028.

Genome annotation databases

EnsembliENST00000369710; ENSP00000358724; ENSG00000148834. [P78417-2]
ENST00000369713; ENSP00000358727; ENSG00000148834. [P78417-1]
ENST00000539281; ENSP00000441488; ENSG00000148834. [P78417-3]
GeneIDi9446.
KEGGihsa:9446.
UCSCiuc021pxr.2. human. [P78417-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90313 mRNA. Translation: AAB70109.1.
AF212303 mRNA. Translation: AAF73376.1.
AY817669 Genomic DNA. Translation: AAV68046.1.
BX537431 mRNA. Translation: CAD97673.1. Different initiation.
AL139341 Genomic DNA. Translation: CAI17224.1.
CH471066 Genomic DNA. Translation: EAW49601.1.
CH471066 Genomic DNA. Translation: EAW49602.1.
CH471066 Genomic DNA. Translation: EAW49603.1.
BC000127 mRNA. Translation: AAH00127.1.
CCDSiCCDS53572.1. [P78417-2]
CCDS53573.1. [P78417-3]
CCDS7555.1. [P78417-1]
RefSeqiNP_001177931.1. NM_001191002.1. [P78417-2]
NP_001177932.1. NM_001191003.1. [P78417-3]
NP_004823.1. NM_004832.2. [P78417-1]
UniGeneiHs.190028.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EEMX-ray2.00A1-241[»]
3LFLX-ray2.10A/B/C1-241[»]
3VLNX-ray1.70A1-241[»]
4IS0X-ray1.72A1-241[»]
4YQMX-ray2.38A/B/C1-241[»]
4YQUX-ray1.94A/B1-241[»]
4YQVX-ray2.06A/B/C1-241[»]
ProteinModelPortaliP78417.
SMRiP78417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114836. 35 interactors.
IntActiP78417. 8 interactors.
MINTiMINT-1384709.
STRINGi9606.ENSP00000358727.

Chemistry databases

BindingDBiP78417.
ChEMBLiCHEMBL3174.
DrugBankiDB00143. Glutathione.
DB00163. Vitamin E.

PTM databases

iPTMnetiP78417.
PhosphoSitePlusiP78417.
SwissPalmiP78417.

Polymorphism and mutation databases

BioMutaiGSTO1.
DMDMi6016173.

2D gel databases

OGPiP78417.
UCD-2DPAGEP78417.

Proteomic databases

EPDiP78417.
PaxDbiP78417.
PeptideAtlasiP78417.
PRIDEiP78417.
TopDownProteomicsiP78417-1. [P78417-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369710; ENSP00000358724; ENSG00000148834. [P78417-2]
ENST00000369713; ENSP00000358727; ENSG00000148834. [P78417-1]
ENST00000539281; ENSP00000441488; ENSG00000148834. [P78417-3]
GeneIDi9446.
KEGGihsa:9446.
UCSCiuc021pxr.2. human. [P78417-1]

Organism-specific databases

CTDi9446.
DisGeNETi9446.
GeneCardsiGSTO1.
HGNCiHGNC:13312. GSTO1.
HPAiHPA037603.
HPA037604.
MIMi605482. gene.
neXtProtiNX_P78417.
OpenTargetsiENSG00000148834.
PharmGKBiPA133787054.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0406. Eukaryota.
ENOG410XSIX. LUCA.
GeneTreeiENSGT00390000005479.
HOGENOMiHOG000006560.
HOVERGENiHBG051853.
InParanoidiP78417.
KOiK00799.
OMAiSTITCEY.
OrthoDBiEOG091G0IEA.
PhylomeDBiP78417.
TreeFamiTF105325.

Enzyme and pathway databases

BioCyciMetaCyc:HS07564-MONOMER.
ZFISH:HS07564-MONOMER.
BRENDAi1.20.4.2. 2681.
1.8.5.1. 2681.
2.5.1.18. 2681.
ReactomeiR-HSA-156581. Methylation.
R-HSA-156590. Glutathione conjugation.
R-HSA-196836. Vitamin C (ascorbate) metabolism.

Miscellaneous databases

ChiTaRSiGSTO1. human.
EvolutionaryTraceiP78417.
GeneWikiiGSTO1.
GenomeRNAii9446.
PROiP78417.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000148834.
CleanExiHS_GSTO1.
ExpressionAtlasiP78417. baseline and differential.
GenevisibleiP78417. HS.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005442. GST_omega.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01625. GSTRNSFRASEO.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTO1_HUMAN
AccessioniPrimary (citable) accession number: P78417
Secondary accession number(s): D3DRA3
, F5H7H0, Q5TA03, Q7Z3T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 178 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.