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P78410 (BT3A2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Butyrophilin subfamily 3 member A2
Gene names
Name:BTN3A2
Synonyms:BT3.2, BTF3, BTF4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in T-cell responses in the adaptive immune response. Inhibits the release of IFNG from activated T-cells. Ref.9 Ref.10

Subunit structure

Homodimer. Ref.11

Subcellular location

Cell membrane; Single-pass type I membrane protein Ref.9 Ref.10.

Tissue specificity

Detected in T-cells and natural killer cells. Ref.9

Post-translational modification

N-glycosylated. Ref.8

Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence caution

The sequence AAB53424.1 differs from that shown. Reason: Frameshift at position 317.

The sequence AAC02652.1 differs from that shown. Reason: Frameshift at position 334.

The sequence AAC02655.1 differs from that shown. Reason: Frameshift at position 334.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P78410-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P78410-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MKMASSLAFLLLNFHVSLLLVQLLTPCS → MGIPR
Note: No experimental confirmation available.
Isoform 3 (identifier: P78410-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 334305Butyrophilin subfamily 3 member A2
PRO_0000014533

Regions

Topological domain30 – 248219Extracellular Potential
Transmembrane249 – 26921Helical; Potential
Topological domain270 – 33465Cytoplasmic Potential
Domain30 – 139110Ig-like V-type
Coiled coil272 – 31948 Potential

Amino acid modifications

Modified residue2821Phosphoserine Ref.7
Modified residue2861Phosphoserine Ref.7
Glycosylation1151N-linked (GlcNAc...) Potential
Disulfide bond52 ↔ 126 Ref.11
Disulfide bond166 ↔ 220 Ref.11

Natural variations

Alternative sequence1 – 4242Missing in isoform 3.
VSP_045906
Alternative sequence1 – 2828MKMAS…LTPCS → MGIPR in isoform 2.
VSP_045907
Natural variant1671R → T.
Corresponds to variant rs9379861 [ dbSNP | Ensembl ].
VAR_049833
Natural variant1811N → D. Ref.4
Corresponds to variant rs9358936 [ dbSNP | Ensembl ].
VAR_026211
Natural variant1821A → T. Ref.4
Corresponds to variant rs12205731 [ dbSNP | Ensembl ].
VAR_049834
Natural variant2111R → K.
Corresponds to variant rs35183513 [ dbSNP | Ensembl ].
VAR_049835
Natural variant3071S → N. Ref.4
Corresponds to variant rs13216828 [ dbSNP | Ensembl ].
VAR_049836

Experimental info

Sequence conflict631M → L in BAG64615. Ref.4
Sequence conflict1801S → G in AAF76140. Ref.3
Sequence conflict211 – 2122RG → KS in AAF76140. Ref.3
Sequence conflict3271S → L in AAC02655. Ref.2

Secondary structure

....................................... 334
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 2005. Version 2.
Checksum: 940519D57F95EEE4

FASTA33436,428
        10         20         30         40         50         60 
MKMASSLAFL LLNFHVSLLL VQLLTPCSAQ FSVLGPSGPI LAMVGEDADL PCHLFPTMSA 

        70         80         90        100        110        120 
ETMELKWVSS SLRQVVNVYA DGKEVEDRQS APYRGRTSIL RDGITAGKAA LRIHNVTASD 

       130        140        150        160        170        180 
SGKYLCYFQD GDFYEKALVE LKVAALGSNL HVEVKGYEDG GIHLECRSTG WYPQPQIQWS 

       190        200        210        220        230        240 
NAKGENIPAV EAPVVADGVG LYEVAASVIM RGGSGEGVSC IIRNSLLGLE KTASISIADP 

       250        260        270        280        290        300 
FFRSAQPWIA ALAGTLPILL LLLAGASYFL WRQQKEITAL SSEIESEQEM KEMGYAATER 

       310        320        330 
EISLRESLQE ELKRKKIQYL TRGEESSSDT NKSA 

« Hide

Isoform 2 [UniParc].

Checksum: BCF5177F02F440F4
Show »

FASTA31133,910
Isoform 3 [UniParc].

Checksum: 85C66916BF5A9195
Show »

FASTA29232,016

References

« Hide 'large scale' references
[1]"A 1.1-Mb transcript map of the hereditary hemochromatosis locus."
Ruddy D.A., Kronmal G.S., Lee V.K., Mintier G.A., Quintana L., Domingo R. Jr., Meyer N.C., Irrinki A., McClelland E.E., Fullan A., Mapa F.A., Moore T., Thomas W., Loeb D.B., Harmon C., Tsuchihashi Z., Wolff R.K., Schatzman R.C., Feder J.N.
Genome Res. 7:441-456(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning, localization, and structure of new members of the butyrophilin gene family in the juxta-telomeric region of the major histocompatibility complex."
Tazi-Ahnini R., Henry J., Offer C., Bouissou-Bouchouata C., Mather I.H., Pontarotti P.
Immunogenetics 47:55-63(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
[3]"The cluster of BTN genes in the extended major histocompatibility complex."
Rhodes D.A., Stammers M., Malcherek G., Beck S., Trowsdale J.
Genomics 71:351-362(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS ASP-181; THR-182 AND ASN-307.
Tissue: Thymus and Tongue.
[5]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta and Skin.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-282 AND SER-286, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[8]"Stimulation of human butyrophilin 3 molecules results in negative regulation of cellular immunity."
Yamashiro H., Yoshizaki S., Tadaki T., Egawa K., Seo N.
J. Leukoc. Biol. 88:757-767(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION.
[9]"Differential role for CD277 as a co-regulator of the immune signal in T and NK cells."
Messal N., Mamessier E., Sylvain A., Celis-Gutierrez J., Thibult M.L., Chetaille B., Firaguay G., Pastor S., Guillaume Y., Wang Q., Hirsch I., Nunes J.A., Olive D.
Eur. J. Immunol. 41:3443-3454(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]"Key implication of CD277/butyrophilin-3 (BTN3A) in cellular stress sensing by a major human gammadelta T-cell subset."
Harly C., Guillaume Y., Nedellec S., Peigne C.M., Monkkonen H., Monkkonen J., Li J., Kuball J., Adams E.J., Netzer S., Dechanet-Merville J., Leger A., Herrmann T., Breathnach R., Olive D., Bonneville M., Scotet E.
Blood 120:2269-2279(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]"The molecular basis for modulation of human Vgamma9Vdelta2 T cell responses by CD277/butyrophilin-3 (BTN3A)-specific antibodies."
Palakodeti A., Sandstrom A., Sundaresan L., Harly C., Nedellec S., Olive D., Scotet E., Bonneville M., Adams E.J.
J. Biol. Chem. 287:32780-32790(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) OF 30-239, SUBUNIT, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U90546 mRNA. Translation: AAB53424.1. Frameshift.
U90144 mRNA. Translation: AAC02652.1. Frameshift.
U97499, U97498 Genomic DNA. Translation: AAC02655.1. Frameshift.
AF257505 mRNA. Translation: AAF76140.1.
AK299417 mRNA. Translation: BAG61399.1.
AK303600 mRNA. Translation: BAG64615.1.
AL021917 Genomic DNA. Translation: CAA17277.2.
BC002832 mRNA. Translation: AAH02832.3.
BC020214 mRNA. Translation: AAH20214.3.
CCDSCCDS4605.1. [P78410-1]
CCDS56399.1. [P78410-2]
CCDS56400.1. [P78410-3]
RefSeqNP_001184175.1. NM_001197246.2. [P78410-1]
NP_001184176.1. NM_001197247.2. [P78410-1]
NP_001184177.1. NM_001197248.2. [P78410-2]
NP_001184178.1. NM_001197249.2. [P78410-3]
NP_008978.2. NM_007047.4. [P78410-1]
XP_005248884.1. XM_005248827.1. [P78410-1]
UniGeneHs.376046.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4F8QX-ray2.38A30-239[»]
ProteinModelPortalP78410.
SMRP78410. Positions 30-239.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116293. 1 interaction.
STRING9606.ENSP00000348751.

PTM databases

PhosphoSiteP78410.

Polymorphism databases

DMDM67462189.

Proteomic databases

MaxQBP78410.
PaxDbP78410.
PRIDEP78410.

Protocols and materials databases

DNASU11118.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000356386; ENSP00000348751; ENSG00000186470. [P78410-1]
ENST00000377708; ENSP00000366937; ENSG00000186470. [P78410-1]
ENST00000396934; ENSP00000380140; ENSG00000186470. [P78410-2]
ENST00000396948; ENSP00000380152; ENSG00000186470. [P78410-1]
ENST00000508906; ENSP00000442687; ENSG00000186470. [P78410-3]
ENST00000527422; ENSP00000432138; ENSG00000186470. [P78410-1]
GeneID11118.
KEGGhsa:11118.
UCSCuc003nhp.3. human. [P78410-1]

Organism-specific databases

CTD11118.
GeneCardsGC06P026365.
HGNCHGNC:1139. BTN3A2.
HPACAB019392.
MIM613594. gene.
neXtProtNX_P78410.
PharmGKBPA25460.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG314774.
HOGENOMHOG000230860.
HOVERGENHBG050747.
InParanoidP78410.
KOK06712.
OMAKESTIHI.
PhylomeDBP78410.
TreeFamTF331083.

Gene expression databases

ArrayExpressP78410.
BgeeP78410.
CleanExHS_BTF3.
HS_BTN3A2.
GenevestigatorP78410.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSBTN3A2. human.
GenomeRNAi11118.
NextBio42256.
PMAP-CutDBP78410.
PROP78410.
SOURCESearch...

Entry information

Entry nameBT3A2_HUMAN
AccessionPrimary (citable) accession number: P78410
Secondary accession number(s): B4DRT7 expand/collapse secondary AC list , B4E103, F5H791, F8W6E0, O00477, O15338, O75658, Q76PA0, Q9BU81, Q9NR44
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM