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Protein

Melanoma antigen preferentially expressed in tumors

Gene

PRAME

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.1 Publication

Miscellaneous

Tumor antigen recognized by cytolytic T lymphocytes.

GO - Molecular functioni

  • retinoic acid receptor binding Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell differentiation Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of cell differentiation Source: UniProtKB
  • negative regulation of retinoic acid receptor signaling pathway Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • regulation of growth Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionRepressor
Biological processApoptosis, Differentiation, Growth regulation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma antigen preferentially expressed in tumorsCurated
Alternative name(s):
Opa-interacting protein 4
Short name:
OIP-4
Preferentially expressed antigen of melanoma
Gene namesi
Name:PRAMEImported
Synonyms:MAPEImported, OIP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000185686.17.
HGNCiHGNC:9336. PRAME.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi470 – 471LL → VV: Loss of interaction with RARA and defective in repressing RARA signaling. 1 Publication2

Organism-specific databases

DisGeNETi23532.
OpenTargetsiENSG00000185686.
PharmGKBiPA33698.

Polymorphism and mutation databases

BioMutaiPRAME.
DMDMi6685631.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001569731 – 509Melanoma antigen preferentially expressed in tumorsAdd BLAST509

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP78395.
MaxQBiP78395.
PaxDbiP78395.
PeptideAtlasiP78395.
PRIDEiP78395.

PTM databases

iPTMnetiP78395.
PhosphoSitePlusiP78395.

Expressioni

Tissue specificityi

Expressed in testis. Detected in samples of kidney, brain and skin.1 Publication

Gene expression databases

BgeeiENSG00000185686.
CleanExiHS_PRAME.
ExpressionAtlasiP78395. baseline and differential.
GenevisibleiP78395. HS.

Organism-specific databases

HPAiHPA045153.

Interactioni

Subunit structurei

Interacts with RARA (via the ligand-binding domain); the interaction is direct and ligand (retinoic acid)-dependent. Interacts with EZH2; required to repress RAR signaling.1 Publication

GO - Molecular functioni

  • retinoic acid receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi117078. 50 interactors.
CORUMiP78395.
IntActiP78395. 17 interactors.
MINTiMINT-1034364.
STRINGi9606.ENSP00000381726.

Structurei

3D structure databases

ProteinModelPortaliP78395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati116 – 145LRR 1; degenerateBy similarityAdd BLAST30
Repeati207 – 231LRR 2; degenerateBy similarityAdd BLAST25
Repeati232 – 258LRR 3; degenerateBy similarityAdd BLAST27
Repeati259 – 294LRR 4; degenerateBy similarityAdd BLAST36
Repeati295 – 320LRR 5By similarityAdd BLAST26
Repeati321 – 352LRR 6By similarityAdd BLAST32
Repeati353 – 371LRR 7By similarityAdd BLAST19
Repeati377 – 404LRR 8By similarityAdd BLAST28
Repeati405 – 429LRR 9By similarityAdd BLAST25

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni416 – 509Mediates interaction with RARA1 PublicationAdd BLAST94

Sequence similaritiesi

Belongs to the PRAME family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG410J8VX. Eukaryota.
ENOG411194M. LUCA.
GeneTreeiENSGT00760000119028.
HOGENOMiHOG000231112.
InParanoidiP78395.
OMAiWTVWSGT.
OrthoDBiEOG091G05PP.
PhylomeDBiP78395.
TreeFamiTF332708.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiView protein in InterPro
IPR032675. L_dom-like.
IPR026271. PRAME_family.
PIRSFiPIRSF038286. PRAME. 1 hit.

Sequencei

Sequence statusi: Complete.

P78395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERRRLWGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR
60 70 80 90 100
ELFPPLFMAA FDGRHSQTLK AMVQAWPFTC LPLGVLMKGQ HLHLETFKAV
110 120 130 140 150
LDGLDVLLAQ EVRPRRWKLQ VLDLRKNSHQ DFWTVWSGNR ASLYSFPEPE
160 170 180 190 200
AAQPMTKKRK VDGLSTEAEQ PFIPVEVLVD LFLKEGACDE LFSYLIEKVK
210 220 230 240 250
RKKNVLRLCC KKLKIFAMPM QDIKMILKMV QLDSIEDLEV TCTWKLPTLA
260 270 280 290 300
KFSPYLGQMI NLRRLLLSHI HASSYISPEK EEQYIAQFTS QFLSLQCLQA
310 320 330 340 350
LYVDSLFFLR GRLDQLLRHV MNPLETLSIT NCRLSEGDVM HLSQSPSVSQ
360 370 380 390 400
LSVLSLSGVM LTDVSPEPLQ ALLERASATL QDLVFDECGI TDDQLLALLP
410 420 430 440 450
SLSHCSQLTT LSFYGNSISI SALQSLLQHL IGLSNLTHVL YPVPLESYED
460 470 480 490 500
IHGTLHLERL AYLHARLREL LCELGRPSMV WLSANPCPHC GDRTFYDPEP

ILCPCFMPN
Length:509
Mass (Da):57,890
Last modified:May 1, 1997 - v1
Checksum:iB5FFF3E7F7E82606
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti452H → D in AAC39560 (PubMed:9466265).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0212587W → R2 PublicationsCorresponds to variant dbSNP:rs2266988Ensembl.1
Natural variantiVAR_062137218M → V. Corresponds to variant dbSNP:rs41277507Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65011 mRNA. Translation: AAC51160.1.
CR456549 mRNA. Translation: CAG30435.1.
AK312769 mRNA. Translation: BAG35634.1.
CH471095 Genomic DNA. Translation: EAW59518.1.
BC014074 mRNA. Translation: AAH14074.1.
BC022008 mRNA. Translation: AAH22008.1.
BC039731 mRNA. Translation: AAH39731.1.
AF025440 mRNA. Translation: AAC39560.1.
CCDSiCCDS13801.1.
RefSeqiNP_001278644.1. NM_001291715.1.
NP_001278645.1. NM_001291716.1.
NP_001278646.1. NM_001291717.1.
NP_001278648.1. NM_001291719.1.
NP_001305055.1. NM_001318126.1.
NP_001305056.1. NM_001318127.1.
NP_006106.1. NM_006115.4.
NP_996836.1. NM_206953.2.
NP_996837.1. NM_206954.2.
NP_996838.1. NM_206955.2.
NP_996839.1. NM_206956.2.
UniGeneiHs.30743.

Genome annotation databases

EnsembliENST00000398741; ENSP00000381726; ENSG00000185686.
ENST00000398743; ENSP00000381728; ENSG00000185686.
ENST00000402697; ENSP00000385198; ENSG00000185686.
ENST00000405655; ENSP00000384343; ENSG00000185686.
ENST00000539862; ENSP00000445097; ENSG00000275013.
ENST00000543184; ENSP00000445675; ENSG00000185686.
ENST00000617728; ENSP00000484066; ENSG00000275013.
ENST00000626503; ENSP00000486485; ENSG00000275013.
ENST00000628830; ENSP00000486330; ENSG00000275013.
GeneIDi23532.
KEGGihsa:23532.
UCSCiuc032qia.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPRAME_HUMAN
AccessioniPrimary (citable) accession number: P78395
Secondary accession number(s): B2R6Y7, O43481, Q8IXN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: September 27, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families