Reviewed, UniProtKB/Swiss-Prot P78368 (KC1G2_HUMAN)

Names and origin

Protein names

Recommended name:
    Casein kinase I isoform gamma-2
      Short name=CKI-gamma 2
    EC=2.7.11.1

Gene names

Name:

CSNK1G2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Testis.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Ref.1 Traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 415

415

Casein kinase I isoform gamma-2

PRO_0000192842

Regions

Domain

46 – 316

271

Protein kinase

Nucleotide binding

52 – 60

9

ATP By similarity

Sites

Active site

165

1

Proton acceptor By similarity

Binding site

75

1

ATP By similarity

Amino acid modifications

Modified residue

366

1

Phosphoserine Ref.5

Modified residue

367

1

Phosphothreonine Ref.5

Natural variations

Natural variant

189

1

F → L Ref.6

VAR_042086

Natural variant

194

1

E → G Ref.6

VAR_042087

Natural variant

196

1

I → T Ref.6

VAR_042088

Natural variant

206

1

Y → C Ref.6

VAR_042089

Natural variant

206

1

Y → H Ref.6

VAR_042090

Natural variant

207

1

R → S Ref.6

VAR_042091

Natural variant

208

1

E → Q Ref.6

VAR_042092

Natural variant

217

1

R → C Ref.6

VAR_042093

Natural variant

223

1

T → M Ref.6

VAR_042094

Experimental info

Sequence conflict

113

1

K → N in AAP88924. Ref.2

Sequence conflict

113

1

K → N in AAH20972. Ref.4

Secondary structure

1

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415

Helix Strand Turn

Details...

Beta strand

46 – 51

6

Beta strand

60 – 65

6

Turn

66 – 68

3

Beta strand

71 – 78

8

Helix

86 – 95

10

Turn

96 – 98

3

Beta strand

105 – 111

7

Beta strand

114 – 120

7

Helix

126 – 132

7

Turn

133 – 135

3

Helix

139 – 158

20

Helix

168 – 170

3

Beta strand

171 – 173

3

Turn

179 – 182

4

Beta strand

183 – 186

4

Beta strand

193 – 196

4

Turn

198 – 200

3

Turn

216 – 218

3

Helix

221 – 224

4

Helix

231 – 247

17

Turn

251 – 254

4

Helix

260 – 272

13

Helix

276 – 279

4

Turn

280 – 282

3

Helix

285 – 295

11

Helix

305 – 318

14

Turn

328 – 331

4

Sequences

	Sequence		Length	Mass (Da)
	P78368-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 036A39148A1DA038	FASTA	415	47,457
	10 20 30 40 50 60 MDFDKKGGKG ETEEGRRMSK AGGGRSSHGI RSSGTSSGVL MVGPNFRVGK KIGCGNFGEL 70 80 90 100 110 120 RLGKNLYTNE YVAIKLEPIK SRAPQLHLEY RFYKQLSATE GVPQVYYFGP CGKYNAMVLE 130 140 150 160 170 180 LLGPSLEDLF DLCDRTFTLK TVLMIAIQLI TRMEYVHTKS LIYRDVKPEN FLVGRPGTKR 190 200 210 220 230 240 QHAIHIIDFG LAKEYIDPET KKHIPYREHK SLTGTARYMS INTHLGKEQS RRDDLEALGH 250 260 270 280 290 300 MFMYFLRGSL PWQGLKADTL KERYQKIGDT KRATPIEVLC ENFPEEMATY LRYVRRLDFF 310 320 330 340 350 360 EKPDYDYLRK LFTDLFDRSG FVFDYEYDWA GKPLPTPIGT VHTDLPSQPQ LRDKTQPHSK 370 380 390 400 410 NQALNSTNGE LNADDPTAGH SNAPITAPAE VEVADETKCC CFFKRRKRKS LQRHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal mapping of human casein kinase I gamma 2 (CSNK1G2)." Kitabayashi A.N., Kusuda J., Hirai M., Hashimoto K. Genomics 46:133-137(1997) [PubMed: 9403068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Muscle.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND THR-367, MASS SPECTROMETRY.
[6]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-189; GLY-194; THR-196; CYS-206; HIS-206; SER-207; GLN-208; CYS-217 AND MET-223.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U89896 mRNA. Translation: AAB88627.1.
BT009922 mRNA. Translation: AAP88924.1.
AF001177 Transcribed RNA. Translation: AAC00212.1.
AC005306 Genomic DNA. Translation: AAC26983.1.
BC018693 mRNA. Translation: AAH18693.1.
BC018699 mRNA. Translation: AAH18699.1.
BC020972 mRNA. Translation: AAH20972.1.

IPI

IPI00297767.

RefSeq

NP_001310.3.

UniGene

Hs.651905

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2C47	X-ray	2.40	A/B/C/D	43-353	[»]

SMR

P78368. Positions 39-338.

ModBase

Search...

Protein-protein interaction databases

IntAct

P78368. 2 interactions.

PTM databases

PhosphoSite

P78368.

Proteomic databases

PRIDE

P78368.

Genome annotation databases

Ensembl

ENSG00000133275. Homo sapiens. [Contig view]

GeneID

1455.

KEGG

hsa:1455.

Organism-specific databases

GeneCards

GC19P001920.

HGNC

HGNC:2455. CSNK1G2.

MIM

602214. gene.

PharmGKB

PA26955.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P78368.

HOVERGEN

P78368.

OMA

P78368. GYVFDYE.

Enzyme and pathway databases

BRENDA

2.7.11.1. 247.

Pathway_Interaction_DB

foxopathway. FoxO family signaling.
hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins.

Gene expression databases

ArrayExpress

P78368.

Bgee

P78368.

CleanEx

HS_CSNK1G2.

GermOnline

ENSG00000133275. Homo sapiens.

Family and domain databases

InterPro

IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
[Graphical view]

Pfam

PF00069. Pkinase. 1 hit.
[Graphical view]

ProDom

PD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

5975.

SOURCE

Search...

Entry information

Entry name

KC1G2_HUMAN

Accession

Primary (citable) accession number: P78368
Secondary accession number(s): O00704, Q8WUB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)