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P78356

- PI42B_HUMAN

UniProt

P78356 - PI42B_HUMAN

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Protein
Phosphatidylinositol 5-phosphate 4-kinase type-2 beta
Gene
PIP4K2B, PIP5K2B
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Kineticsi

  1. KM=30 µM for phosphatidylinositol-5- phosphate1 Publication

Vmax=0.2 pmol/min/µg enzyme

GO - Molecular functioni

  1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: MGI
  2. 1-phosphatidylinositol-5-phosphate 4-kinase activity Source: UniProtKB-EC
  3. ATP binding Source: UniProtKB-KW
  4. receptor signaling protein activity Source: ProtInc
Complete GO annotation...

GO - Biological processi

  1. cell surface receptor signaling pathway Source: ProtInc
  2. intracellular signal transduction Source: GOC
  3. phosphatidylinositol biosynthetic process Source: Reactome
  4. phosphatidylinositol phosphorylation Source: GOC
  5. phospholipid metabolic process Source: Reactome
  6. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_121025. Synthesis of PIPs at the plasma membrane.
SABIO-RKP78356.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (EC:2.7.1.149)
Alternative name(s):
1-phosphatidylinositol 5-phosphate 4-kinase 2-beta
Diphosphoinositide kinase 2-beta
Phosphatidylinositol 5-phosphate 4-kinase type II beta
Short name:
PI(5)P 4-kinase type II beta
Short name:
PIP4KII-beta
PtdIns(5)P-4-kinase isoform 2-beta
Gene namesi
Name:PIP4K2B
Synonyms:PIP5K2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:8998. PIP4K2B.

Subcellular locationi

Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Cell membrane; Peripheral membrane protein By similarity. Nucleus. Cytoplasm
Note: Associated with the plasma membrane and the endoplasmic reticulum By similarity.1 Publication

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162399640.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 416415Phosphatidylinositol 5-phosphate 4-kinase type-2 beta
PRO_0000185470Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei150 – 1501N6-acetyllysine1 Publication
Modified residuei326 – 3261Phosphoserine2 Publications

Post-translational modificationi

Ubiquitinated by the SPOP/CUL3 complex. Ubiquitination is stimulated by PtdIns5P levels.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP78356.
PaxDbiP78356.
PRIDEiP78356.

PTM databases

PhosphoSiteiP78356.

Expressioni

Tissue specificityi

Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver.1 Publication

Gene expression databases

ArrayExpressiP78356.
BgeeiP78356.
CleanExiHS_PIP4K2B.
GenevestigatoriP78356.

Organism-specific databases

HPAiHPA047875.

Interactioni

Subunit structurei

Homodimer. Binds TNFRSF1A. Interacts with PIP4K2A. Interaction with PIP4K2A suppresses ubiquitination by the SPOP/ CUL3 complex.3 Publications

Protein-protein interaction databases

BioGridi113985. 7 interactions.
IntActiP78356. 4 interactions.
MINTiMINT-2864974.
STRINGi9606.ENSP00000269554.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi41 – 5717
Helixi68 – 714
Beta strandi74 – 807
Beta strandi82 – 854
Beta strandi87 – 893
Beta strandi91 – 988
Helixi100 – 10910
Helixi114 – 1229
Beta strandi139 – 1413
Beta strandi145 – 1506
Helixi154 – 17219
Turni173 – 1764
Beta strandi183 – 1919
Beta strandi194 – 2029
Beta strandi207 – 2093
Beta strandi212 – 2176
Beta strandi221 – 2244
Helixi229 – 2313
Beta strandi232 – 2343
Helixi240 – 2456
Helixi256 – 27419
Beta strandi281 – 2877
Helixi288 – 30114
Turni302 – 3054
Turni347 – 3493
Beta strandi353 – 3553
Beta strandi361 – 3677
Beta strandi394 – 3963
Helixi400 – 41415

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BO1X-ray3.00A/B1-416[»]
DisProtiDP00054.
ProteinModelPortaliP78356.
SMRiP78356. Positions 34-416.

Miscellaneous databases

EvolutionaryTraceiP78356.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 415378PIPK
Add
BLAST

Sequence similaritiesi

Contains 1 PIPK domain.

Phylogenomic databases

eggNOGiCOG5253.
HOGENOMiHOG000007832.
HOVERGENiHBG000072.
InParanoidiP78356.
KOiK00920.
OMAiECENDGL.
OrthoDBiEOG708VZZ.
PhylomeDBiP78356.
TreeFamiTF354315.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProiIPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P78356-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSSNCTSTTA VAVAPLSASK TKTKKKHFVC QKVKLFRASE PILSVLMWGV    50
NHTINELSNV PVPVMLMPDD FKAYSKIKVD NHLFNKENLP SRFKFKEYCP 100
MVFRNLRERF GIDDQDYQNS VTRSAPINSD SQGRCGTRFL TTYDRRFVIK 150
TVSSEDVAEM HNILKKYHQF IVECHGNTLL PQFLGMYRLT VDGVETYMVV 200
TRNVFSHRLT VHRKYDLKGS TVAREASDKE KAKDLPTFKD NDFLNEGQKL 250
HVGEESKKNF LEKLKRDVEF LAQLKIMDYS LLVGIHDVDR AEQEEMEVEE 300
RAEDEECEND GVGGNLLCSY GTPPDSPGNL LSFPRFFGPG EFDPSVDVYA 350
MKSHESSPKK EVYFMAIIDI LTPYDTKKKA AHAAKTVKHG AGAEISTVNP 400
EQYSKRFNEF MSNILT 416
Length:416
Mass (Da):47,378
Last modified:May 1, 1997 - v1
Checksum:i2288CD2883EACEBE
GO
Isoform 2 (identifier: P78356-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-281: FLAQLKIMDYSL → EILVLSPGRRIA
     282-416: Missing.

Note: No experimental confirmation available.

Show »
Length:281
Mass (Da):32,317
Checksum:iD83D7A24BC4B8DA6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei270 – 28112FLAQL…MDYSL → EILVLSPGRRIA in isoform 2.
VSP_010384Add
BLAST
Alternative sequencei282 – 416135Missing in isoform 2.
VSP_010385Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti250 – 2501L → P in AAV38420. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U85245 mRNA. Translation: AAB48596.1.
BT019614 mRNA. Translation: AAV38420.1.
BC027459 mRNA. Translation: AAH27459.1.
CCDSiCCDS11329.1. [P78356-1]
RefSeqiNP_003550.1. NM_003559.4. [P78356-1]
UniGeneiHs.260603.

Genome annotation databases

EnsembliENST00000269554; ENSP00000269554; ENSG00000141720. [P78356-1]
GeneIDi8396.
KEGGihsa:8396.
UCSCiuc002hqs.3. human. [P78356-1]
uc021twj.1. human. [P78356-2]

Polymorphism databases

DMDMi47605991.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U85245 mRNA. Translation: AAB48596.1 .
BT019614 mRNA. Translation: AAV38420.1 .
BC027459 mRNA. Translation: AAH27459.1 .
CCDSi CCDS11329.1. [P78356-1 ]
RefSeqi NP_003550.1. NM_003559.4. [P78356-1 ]
UniGenei Hs.260603.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BO1 X-ray 3.00 A/B 1-416 [» ]
DisProti DP00054.
ProteinModelPortali P78356.
SMRi P78356. Positions 34-416.
ModBasei Search...

Protein-protein interaction databases

BioGridi 113985. 7 interactions.
IntActi P78356. 4 interactions.
MINTi MINT-2864974.
STRINGi 9606.ENSP00000269554.

Chemistry

BindingDBi P78356.
ChEMBLi CHEMBL5667.
GuidetoPHARMACOLOGYi 2162.

PTM databases

PhosphoSitei P78356.

Polymorphism databases

DMDMi 47605991.

Proteomic databases

MaxQBi P78356.
PaxDbi P78356.
PRIDEi P78356.

Protocols and materials databases

DNASUi 8396.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000269554 ; ENSP00000269554 ; ENSG00000141720 . [P78356-1 ]
GeneIDi 8396.
KEGGi hsa:8396.
UCSCi uc002hqs.3. human. [P78356-1 ]
uc021twj.1. human. [P78356-2 ]

Organism-specific databases

CTDi 8396.
GeneCardsi GC17M036921.
HGNCi HGNC:8998. PIP4K2B.
HPAi HPA047875.
MIMi 603261. gene.
neXtProti NX_P78356.
PharmGKBi PA162399640.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5253.
HOGENOMi HOG000007832.
HOVERGENi HBG000072.
InParanoidi P78356.
KOi K00920.
OMAi ECENDGL.
OrthoDBi EOG708VZZ.
PhylomeDBi P78356.
TreeFami TF354315.

Enzyme and pathway databases

Reactomei REACT_121025. Synthesis of PIPs at the plasma membrane.
SABIO-RK P78356.

Miscellaneous databases

ChiTaRSi PIP4K2B. human.
EvolutionaryTracei P78356.
GeneWikii PIP4K2B.
GenomeRNAii 8396.
NextBioi 31424.
PROi P78356.
SOURCEi Search...

Gene expression databases

ArrayExpressi P78356.
Bgeei P78356.
CleanExi HS_PIP4K2B.
Genevestigatori P78356.

Family and domain databases

Gene3Di 3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProi IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view ]
PANTHERi PTHR23086. PTHR23086. 1 hit.
Pfami PF01504. PIP5K. 1 hit.
[Graphical view ]
SMARTi SM00330. PIPKc. 1 hit.
[Graphical view ]
PROSITEi PS51455. PIPK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel interaction between the juxtamembrane region of the p55 tumor necrosis factor receptor and phosphatidylinositol-4-phosphate 5-kinase."
    Castellino A.M., Parker G.J., Boronenkov I.V., Anderson R.A., Chao M.V.
    J. Biol. Chem. 272:5861-5870(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH TNFRSF1A, TISSUE SPECIFICITY.
    Tissue: Fetal brain.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Lung.
  4. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-150, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "PIP4Kbeta interacts with and modulates nuclear localization of the high-activity PtdIns5P-4-kinase isoform PIP4Kalpha."
    Bultsma Y., Keune W.-J., Divecha N.
    Biochem. J. 430:223-235(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH PIP4K2A, SUBCELLULAR LOCATION, UBIQUITINATION, IDENTIFICATION BY MASS SPECTROMETRY.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  11. "Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation."
    Rao V.D., Misra S., Boronenkov I.V., Anderson R.A., Hurley J.H.
    Cell 94:829-839(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 34-416, HOMODIMERIZATION.

Entry informationi

Entry nameiPI42B_HUMAN
AccessioniPrimary (citable) accession number: P78356
Secondary accession number(s): Q5U0E8, Q8TBP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 1, 1997
Last modified: September 3, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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