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Reviewed, UniProtKB/Swiss-Prot P78356 (PI42B_HUMAN)

Last modified June 16, 2009. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Phosphatidylinositol-5-phosphate 4-kinase type-2 beta
    EC=2.7.1.149
Alternative name(s):
    Phosphatidylinositol-5-phosphate 4-kinase type II beta
    1-phosphatidylinositol-5-phosphate 4-kinase 2-beta
    PtdIns(5)P-4-kinase isoform 2-beta
      Short name=PIP4KII-beta
    Diphosphoinositide kinase 2-beta
Gene names
Name: PIP4K2B
Synonyms: PIP5K2B
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Ref.1

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Subunit structure

Homodimer. Binds TNFRSF1A. Ref.6

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Cell membrane; Peripheral membrane protein By similarity. Note: Associated with the plasma membrane and the endoplasmic reticulum By similarity.

Tissue specificity

Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver. Ref.1

Post-translational modification

Phosphorylated on serine residues By similarity.

Sequence similarities

Contains 1 PI5K domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P78356-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P78356-2)

The sequence of this isoform differs from the canonical sequence as follows:
     270-281: FLAQLKIMDYSL → EILVLSPGRRIA
     282-416: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Phosphatidylinositol-5-phosphate 4-kinase type-2 beta
PRO_0000185470

Regions

Domain119 – 415297PI5K

Amino acid modifications

Modified residue981Phosphotyrosine Ref.4
Modified residue3221Phosphothreonine Ref.5
Modified residue3261Phosphoserine Ref.5

Natural variations

Alternative sequence270 – 28112FLAQL…MDYSL → EILVLSPGRRIA in isoform 2.
VSP_010384
Alternative sequence282 – 416135Missing in isoform 2.
VSP_010385

Experimental info

Sequence conflict2501L → P in AAV38420. Ref.2

Secondary structure

....................................................... 416
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 2288CD2883EACEBE

FASTA41647,378
        10         20         30         40         50         60 
MSSNCTSTTA VAVAPLSASK TKTKKKHFVC QKVKLFRASE PILSVLMWGV NHTINELSNV 

        70         80         90        100        110        120 
PVPVMLMPDD FKAYSKIKVD NHLFNKENLP SRFKFKEYCP MVFRNLRERF GIDDQDYQNS 

       130        140        150        160        170        180 
VTRSAPINSD SQGRCGTRFL TTYDRRFVIK TVSSEDVAEM HNILKKYHQF IVECHGNTLL 

       190        200        210        220        230        240 
PQFLGMYRLT VDGVETYMVV TRNVFSHRLT VHRKYDLKGS TVAREASDKE KAKDLPTFKD 

       250        260        270        280        290        300 
NDFLNEGQKL HVGEESKKNF LEKLKRDVEF LAQLKIMDYS LLVGIHDVDR AEQEEMEVEE 

       310        320        330        340        350        360 
RAEDEECEND GVGGNLLCSY GTPPDSPGNL LSFPRFFGPG EFDPSVDVYA MKSHESSPKK 

       370        380        390        400        410 
EVYFMAIIDI LTPYDTKKKA AHAAKTVKHG AGAEISTVNP EQYSKRFNEF MSNILT 

« Hide

Isoform 2.

Checksum: D83D7A24BC4B8DA6
Show »

FASTA28132,317

References

« Hide 'large scale' references
[1]"A novel interaction between the juxtamembrane region of the p55 tumor necrosis factor receptor and phosphatidylinositol-4-phosphate 5-kinase."
Castellino A.M., Parker G.J., Boronenkov I.V., Anderson R.A., Chao M.V.
J. Biol. Chem. 272:5861-5870(1997) [PubMed: 9038203] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH TNFRSF1A, TISSUE SPECIFICITY.
Tissue: Fetal brain.
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Lung.
[4]"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer."
Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. expand/collapse author list , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.
Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-98, MASS SPECTROMETRY.
[5]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-322 AND SER-326, MASS SPECTROMETRY.
[6]"Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation."
Rao V.D., Misra S., Boronenkov I.V., Anderson R.A., Hurley J.H.
Cell 94:829-839(1998) [PubMed: 9753329] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 34-416, HOMODIMERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

U85245 mRNA. Translation: AAB48596.1.
BT019614 mRNA. Translation: AAV38420.1.
BC027459 mRNA. Translation: AAH27459.1.
IPIIPI00216470.
IPI00235352.
RefSeqNP_003550.1.
UniGeneHs.260603

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1BO1X-ray3.00A/B1-416[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP78356. 2 interactions.

PTM databases

PhosphoSiteP78356.

Proteomic databases

PRIDEP78356.

Genome annotation databases

EnsemblENSG00000141720. Homo sapiens. [Contig view]
GeneID8396.
KEGGhsa:8396.

Organism-specific databases

GeneCardsGC17M034175.
H-InvDBHIX0013764.
HGNCHGNC:8998. PIP4K2B.
MIM603261. gene.
PharmGKBPA33331.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP78356.
HOVERGENP78356.
OMAP78356. ECENDGM.

Enzyme and pathway databases

BRENDA2.7.1.149. 247.

Gene expression databases

ArrayExpressP78356.
BgeeP78356.
CleanExHS_PIP4K2B.
GermOnlineENSG00000141720. Homo sapiens.

Family and domain databases

InterProIPR002498. PInositol-4-P-5-kinase_core.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERPTHR23086. PIP5K. 1 hit.
PfamPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTSM00330. PIPKc. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio31424.
SOURCESearch...

Entry information

Entry namePI42B_HUMAN
AccessionPrimary (citable) accession number: P78356
Secondary accession number(s): Q5U0E8, Q8TBP2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 1, 1997
Last modified: June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents