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Protein

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta

Gene

PIP4K2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Kineticsi

  1. KM=30 µM for phosphatidylinositol-5- phosphate1 Publication
  1. Vmax=0.2 pmol/min/µg enzyme1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell surface receptor signaling pathway Source: ProtInc
  • phosphatidylinositol biosynthetic process Source: Reactome
  • phospholipid metabolic process Source: Reactome
  • positive regulation of autophagosome assembly Source: ParkinsonsUK-UCL
  • regulation of autophagy Source: ParkinsonsUK-UCL
  • regulation of phosphatidylinositol 3-kinase signaling Source: Reactome

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.149. 2681.
ReactomeiR-HSA-1660499. Synthesis of PIPs at the plasma membrane.
R-HSA-6811555. PI5P Regulates TP53 Acetylation.
R-HSA-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-HSA-8847453. Synthesis of PIPs in the nucleus.
SABIO-RKiP78356.
SIGNORiP78356.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (EC:2.7.1.149)
Alternative name(s):
1-phosphatidylinositol 5-phosphate 4-kinase 2-beta
Diphosphoinositide kinase 2-beta
Phosphatidylinositol 5-phosphate 4-kinase type II beta
Short name:
PI(5)P 4-kinase type II beta
Short name:
PIP4KII-beta
PtdIns(5)P-4-kinase isoform 2-beta
Gene namesi
Name:PIP4K2B
Synonyms:PIP5K2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000276293.4.
HGNCiHGNC:8998. PIP4K2B.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8396.
OpenTargetsiENSG00000276293.
PharmGKBiPA162399640.

Chemistry databases

ChEMBLiCHEMBL5667.
GuidetoPHARMACOLOGYi2162.

Polymorphism and mutation databases

BioMutaiPIP4K2B.
DMDMi47605991.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001854702 – 416Phosphatidylinositol 5-phosphate 4-kinase type-2 betaAdd BLAST415

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei150N6-acetyllysineCombined sources1
Modified residuei322PhosphothreonineBy similarity1
Modified residuei326PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated by the SPOP/CUL3 complex. Ubiquitination is stimulated by PtdIns5P levels.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP78356.
MaxQBiP78356.
PaxDbiP78356.
PeptideAtlasiP78356.
PRIDEiP78356.

PTM databases

iPTMnetiP78356.
PhosphoSitePlusiP78356.

Expressioni

Tissue specificityi

Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver.1 Publication

Gene expression databases

BgeeiENSG00000276293.
CleanExiHS_PIP4K2B.
ExpressionAtlasiP78356. baseline and differential.
GenevisibleiP78356. HS.

Organism-specific databases

HPAiHPA047875.
HPA062220.

Interactioni

Subunit structurei

Homodimer. Binds TNFRSF1A. Interacts with PIP4K2A. Interaction with PIP4K2A suppresses ubiquitination by the SPOP/ CUL3 complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HMBOX1Q6NT765EBI-947090,EBI-2549423

GO - Molecular functioni

  • protein homodimerization activity Source: CAFA

Protein-protein interaction databases

BioGridi113985. 36 interactors.
IntActiP78356. 9 interactors.
MINTiMINT-2864974.
STRINGi9606.ENSP00000269554.

Chemistry databases

BindingDBiP78356.

Structurei

Secondary structure

1416
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi41 – 57Combined sources17
Helixi68 – 72Combined sources5
Beta strandi74 – 82Combined sources9
Beta strandi87 – 89Combined sources3
Beta strandi91 – 99Combined sources9
Helixi100 – 109Combined sources10
Helixi114 – 122Combined sources9
Beta strandi133 – 136Combined sources4
Beta strandi139 – 141Combined sources3
Beta strandi145 – 152Combined sources8
Helixi154 – 173Combined sources20
Turni174 – 176Combined sources3
Beta strandi183 – 191Combined sources9
Beta strandi194 – 202Combined sources9
Beta strandi207 – 209Combined sources3
Beta strandi213 – 217Combined sources5
Beta strandi221 – 224Combined sources4
Turni228 – 232Combined sources5
Beta strandi233 – 235Combined sources3
Beta strandi237 – 239Combined sources3
Helixi240 – 245Combined sources6
Helixi254 – 273Combined sources20
Beta strandi280 – 287Combined sources8
Helixi288 – 303Combined sources16
Turni344 – 346Combined sources3
Turni347 – 349Combined sources3
Beta strandi350 – 352Combined sources3
Beta strandi353 – 355Combined sources3
Beta strandi357 – 359Combined sources3
Beta strandi361 – 368Combined sources8
Beta strandi394 – 396Combined sources3
Helixi401 – 412Combined sources12
Beta strandi414 – 416Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BO1X-ray3.00A/B1-416[»]
3WZZX-ray2.60A/B31-416[»]
3X01X-ray2.15A/B31-416[»]
3X02X-ray2.45A/B31-416[»]
3X03X-ray2.70A/B31-416[»]
3X04X-ray2.60A/B31-416[»]
3X05X-ray2.50A/B31-416[»]
3X06X-ray2.65A/B31-416[»]
3X07X-ray2.60A/B31-416[»]
3X08X-ray2.75A/B31-416[»]
3X09X-ray2.70A/B31-416[»]
3X0AX-ray2.60A/B31-416[»]
3X0BX-ray2.60A/B31-416[»]
3X0CX-ray2.55A/B31-416[»]
DisProtiDP00054.
ProteinModelPortaliP78356.
SMRiP78356.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78356.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 415PIPKPROSITE-ProRule annotationAdd BLAST378

Phylogenomic databases

eggNOGiKOG0229. Eukaryota.
COG5253. LUCA.
GeneTreeiENSGT00760000119184.
HOGENOMiHOG000007832.
HOVERGENiHBG000072.
InParanoidiP78356.
KOiK00920.
OMAiECENDGL.
OrthoDBiEOG091G0857.
PhylomeDBiP78356.
TreeFamiTF354315.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 1 hit.
InterProiView protein in InterPro
IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiView protein in Pfam
PF01504. PIP5K. 1 hit.
SMARTiView protein in SMART
SM00330. PIPKc. 1 hit.
PROSITEiView protein in PROSITE
PS51455. PIPK. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P78356-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSNCTSTTA VAVAPLSASK TKTKKKHFVC QKVKLFRASE PILSVLMWGV
60 70 80 90 100
NHTINELSNV PVPVMLMPDD FKAYSKIKVD NHLFNKENLP SRFKFKEYCP
110 120 130 140 150
MVFRNLRERF GIDDQDYQNS VTRSAPINSD SQGRCGTRFL TTYDRRFVIK
160 170 180 190 200
TVSSEDVAEM HNILKKYHQF IVECHGNTLL PQFLGMYRLT VDGVETYMVV
210 220 230 240 250
TRNVFSHRLT VHRKYDLKGS TVAREASDKE KAKDLPTFKD NDFLNEGQKL
260 270 280 290 300
HVGEESKKNF LEKLKRDVEF LAQLKIMDYS LLVGIHDVDR AEQEEMEVEE
310 320 330 340 350
RAEDEECEND GVGGNLLCSY GTPPDSPGNL LSFPRFFGPG EFDPSVDVYA
360 370 380 390 400
MKSHESSPKK EVYFMAIIDI LTPYDTKKKA AHAAKTVKHG AGAEISTVNP
410
EQYSKRFNEF MSNILT
Length:416
Mass (Da):47,378
Last modified:May 1, 1997 - v1
Checksum:i2288CD2883EACEBE
GO
Isoform 2 (identifier: P78356-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-281: FLAQLKIMDYSL → EILVLSPGRRIA
     282-416: Missing.

Note: No experimental confirmation available.
Show »
Length:281
Mass (Da):32,317
Checksum:iD83D7A24BC4B8DA6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti250L → P in AAV38420 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010384270 – 281FLAQL…MDYSL → EILVLSPGRRIA in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_010385282 – 416Missing in isoform 2. 1 PublicationAdd BLAST135

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85245 mRNA. Translation: AAB48596.1.
BT019614 mRNA. Translation: AAV38420.1.
BC027459 mRNA. Translation: AAH27459.1.
CCDSiCCDS11329.1. [P78356-1]
RefSeqiNP_003550.1. NM_003559.4. [P78356-1]
UniGeneiHs.260603.

Genome annotation databases

EnsembliENST00000613180; ENSP00000480776; ENSG00000277292. [P78356-1]
ENST00000619039; ENSP00000482548; ENSG00000276293. [P78356-1]
GeneIDi8396.
KEGGihsa:8396.
UCSCiuc002hqs.4. human. [P78356-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPI42B_HUMAN
AccessioniPrimary (citable) accession number: P78356
Secondary accession number(s): Q5U0E8, Q8TBP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 1, 1997
Last modified: October 25, 2017
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references