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P78356

- PI42B_HUMAN

UniProt

P78356 - PI42B_HUMAN

Protein

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta

Gene

PIP4K2B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.1 Publication

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

    Kineticsi

    1. KM=30 µM for phosphatidylinositol-5- phosphate1 Publication

    Vmax=0.2 pmol/min/µg enzyme1 Publication

    GO - Molecular functioni

    1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: MGI
    2. 1-phosphatidylinositol-5-phosphate 4-kinase activity Source: UniProtKB-EC
    3. ATP binding Source: UniProtKB-KW
    4. receptor signaling protein activity Source: ProtInc

    GO - Biological processi

    1. cell surface receptor signaling pathway Source: ProtInc
    2. intracellular signal transduction Source: GOC
    3. phosphatidylinositol biosynthetic process Source: Reactome
    4. phosphatidylinositol phosphorylation Source: GOC
    5. phospholipid metabolic process Source: Reactome
    6. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_121025. Synthesis of PIPs at the plasma membrane.
    SABIO-RKP78356.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (EC:2.7.1.149)
    Alternative name(s):
    1-phosphatidylinositol 5-phosphate 4-kinase 2-beta
    Diphosphoinositide kinase 2-beta
    Phosphatidylinositol 5-phosphate 4-kinase type II beta
    Short name:
    PI(5)P 4-kinase type II beta
    Short name:
    PIP4KII-beta
    PtdIns(5)P-4-kinase isoform 2-beta
    Gene namesi
    Name:PIP4K2B
    Synonyms:PIP5K2B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:8998. PIP4K2B.

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity. Nucleus 1 Publication. Cytoplasm 1 Publication
    Note: Associated with the plasma membrane and the endoplasmic reticulum.By similarity

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162399640.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 416415Phosphatidylinositol 5-phosphate 4-kinase type-2 betaPRO_0000185470Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei150 – 1501N6-acetyllysine1 Publication
    Modified residuei326 – 3261Phosphoserine2 Publications

    Post-translational modificationi

    Ubiquitinated by the SPOP/CUL3 complex. Ubiquitination is stimulated by PtdIns5P levels.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP78356.
    PaxDbiP78356.
    PRIDEiP78356.

    PTM databases

    PhosphoSiteiP78356.

    Expressioni

    Tissue specificityi

    Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver.1 Publication

    Gene expression databases

    ArrayExpressiP78356.
    BgeeiP78356.
    CleanExiHS_PIP4K2B.
    GenevestigatoriP78356.

    Organism-specific databases

    HPAiHPA047875.

    Interactioni

    Subunit structurei

    Homodimer. Binds TNFRSF1A. Interacts with PIP4K2A. Interaction with PIP4K2A suppresses ubiquitination by the SPOP/ CUL3 complex.2 Publications

    Protein-protein interaction databases

    BioGridi113985. 7 interactions.
    IntActiP78356. 4 interactions.
    MINTiMINT-2864974.
    STRINGi9606.ENSP00000269554.

    Structurei

    Secondary structure

    1
    416
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi41 – 5717
    Helixi68 – 714
    Beta strandi74 – 807
    Beta strandi82 – 854
    Beta strandi87 – 893
    Beta strandi91 – 988
    Helixi100 – 10910
    Helixi114 – 1229
    Beta strandi139 – 1413
    Beta strandi145 – 1506
    Helixi154 – 17219
    Turni173 – 1764
    Beta strandi183 – 1919
    Beta strandi194 – 2029
    Beta strandi207 – 2093
    Beta strandi212 – 2176
    Beta strandi221 – 2244
    Helixi229 – 2313
    Beta strandi232 – 2343
    Helixi240 – 2456
    Helixi256 – 27419
    Beta strandi281 – 2877
    Helixi288 – 30114
    Turni302 – 3054
    Turni347 – 3493
    Beta strandi353 – 3553
    Beta strandi361 – 3677
    Beta strandi394 – 3963
    Helixi400 – 41415

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BO1X-ray3.00A/B1-416[»]
    DisProtiDP00054.
    ProteinModelPortaliP78356.
    SMRiP78356. Positions 34-416.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP78356.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 415378PIPKPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PIPK domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5253.
    HOGENOMiHOG000007832.
    HOVERGENiHBG000072.
    InParanoidiP78356.
    KOiK00920.
    OMAiECENDGL.
    OrthoDBiEOG708VZZ.
    PhylomeDBiP78356.
    TreeFamiTF354315.

    Family and domain databases

    Gene3Di3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProiIPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view]
    PANTHERiPTHR23086. PTHR23086. 1 hit.
    PfamiPF01504. PIP5K. 1 hit.
    [Graphical view]
    SMARTiSM00330. PIPKc. 1 hit.
    [Graphical view]
    PROSITEiPS51455. PIPK. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P78356-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSNCTSTTA VAVAPLSASK TKTKKKHFVC QKVKLFRASE PILSVLMWGV    50
    NHTINELSNV PVPVMLMPDD FKAYSKIKVD NHLFNKENLP SRFKFKEYCP 100
    MVFRNLRERF GIDDQDYQNS VTRSAPINSD SQGRCGTRFL TTYDRRFVIK 150
    TVSSEDVAEM HNILKKYHQF IVECHGNTLL PQFLGMYRLT VDGVETYMVV 200
    TRNVFSHRLT VHRKYDLKGS TVAREASDKE KAKDLPTFKD NDFLNEGQKL 250
    HVGEESKKNF LEKLKRDVEF LAQLKIMDYS LLVGIHDVDR AEQEEMEVEE 300
    RAEDEECEND GVGGNLLCSY GTPPDSPGNL LSFPRFFGPG EFDPSVDVYA 350
    MKSHESSPKK EVYFMAIIDI LTPYDTKKKA AHAAKTVKHG AGAEISTVNP 400
    EQYSKRFNEF MSNILT 416
    Length:416
    Mass (Da):47,378
    Last modified:May 1, 1997 - v1
    Checksum:i2288CD2883EACEBE
    GO
    Isoform 2 (identifier: P78356-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         270-281: FLAQLKIMDYSL → EILVLSPGRRIA
         282-416: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:281
    Mass (Da):32,317
    Checksum:iD83D7A24BC4B8DA6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti250 – 2501L → P in AAV38420. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei270 – 28112FLAQL…MDYSL → EILVLSPGRRIA in isoform 2. 1 PublicationVSP_010384Add
    BLAST
    Alternative sequencei282 – 416135Missing in isoform 2. 1 PublicationVSP_010385Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U85245 mRNA. Translation: AAB48596.1.
    BT019614 mRNA. Translation: AAV38420.1.
    BC027459 mRNA. Translation: AAH27459.1.
    CCDSiCCDS11329.1. [P78356-1]
    RefSeqiNP_003550.1. NM_003559.4. [P78356-1]
    UniGeneiHs.260603.

    Genome annotation databases

    GeneIDi8396.
    KEGGihsa:8396.
    UCSCiuc002hqs.3. human. [P78356-1]
    uc021twj.1. human. [P78356-2]

    Polymorphism databases

    DMDMi47605991.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U85245 mRNA. Translation: AAB48596.1 .
    BT019614 mRNA. Translation: AAV38420.1 .
    BC027459 mRNA. Translation: AAH27459.1 .
    CCDSi CCDS11329.1. [P78356-1 ]
    RefSeqi NP_003550.1. NM_003559.4. [P78356-1 ]
    UniGenei Hs.260603.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BO1 X-ray 3.00 A/B 1-416 [» ]
    DisProti DP00054.
    ProteinModelPortali P78356.
    SMRi P78356. Positions 34-416.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113985. 7 interactions.
    IntActi P78356. 4 interactions.
    MINTi MINT-2864974.
    STRINGi 9606.ENSP00000269554.

    Chemistry

    BindingDBi P78356.
    ChEMBLi CHEMBL5667.
    GuidetoPHARMACOLOGYi 2162.

    PTM databases

    PhosphoSitei P78356.

    Polymorphism databases

    DMDMi 47605991.

    Proteomic databases

    MaxQBi P78356.
    PaxDbi P78356.
    PRIDEi P78356.

    Protocols and materials databases

    DNASUi 8396.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8396.
    KEGGi hsa:8396.
    UCSCi uc002hqs.3. human. [P78356-1 ]
    uc021twj.1. human. [P78356-2 ]

    Organism-specific databases

    CTDi 8396.
    GeneCardsi GC17M036921.
    HGNCi HGNC:8998. PIP4K2B.
    HPAi HPA047875.
    MIMi 603261. gene.
    neXtProti NX_P78356.
    PharmGKBi PA162399640.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5253.
    HOGENOMi HOG000007832.
    HOVERGENi HBG000072.
    InParanoidi P78356.
    KOi K00920.
    OMAi ECENDGL.
    OrthoDBi EOG708VZZ.
    PhylomeDBi P78356.
    TreeFami TF354315.

    Enzyme and pathway databases

    Reactomei REACT_121025. Synthesis of PIPs at the plasma membrane.
    SABIO-RK P78356.

    Miscellaneous databases

    ChiTaRSi PIP4K2B. human.
    EvolutionaryTracei P78356.
    GeneWikii PIP4K2B.
    GenomeRNAii 8396.
    NextBioi 31424.
    PROi P78356.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P78356.
    Bgeei P78356.
    CleanExi HS_PIP4K2B.
    Genevestigatori P78356.

    Family and domain databases

    Gene3Di 3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProi IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view ]
    PANTHERi PTHR23086. PTHR23086. 1 hit.
    Pfami PF01504. PIP5K. 1 hit.
    [Graphical view ]
    SMARTi SM00330. PIPKc. 1 hit.
    [Graphical view ]
    PROSITEi PS51455. PIPK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel interaction between the juxtamembrane region of the p55 tumor necrosis factor receptor and phosphatidylinositol-4-phosphate 5-kinase."
      Castellino A.M., Parker G.J., Boronenkov I.V., Anderson R.A., Chao M.V.
      J. Biol. Chem. 272:5861-5870(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH TNFRSF1A, TISSUE SPECIFICITY.
      Tissue: Fetal brain.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Lung.
    4. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-150, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "PIP4Kbeta interacts with and modulates nuclear localization of the high-activity PtdIns5P-4-kinase isoform PIP4Kalpha."
      Bultsma Y., Keune W.-J., Divecha N.
      Biochem. J. 430:223-235(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH PIP4K2A, SUBCELLULAR LOCATION, UBIQUITINATION, IDENTIFICATION BY MASS SPECTROMETRY.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    11. "Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation."
      Rao V.D., Misra S., Boronenkov I.V., Anderson R.A., Hurley J.H.
      Cell 94:829-839(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 34-416, HOMODIMERIZATION.

    Entry informationi

    Entry nameiPI42B_HUMAN
    AccessioniPrimary (citable) accession number: P78356
    Secondary accession number(s): Q5U0E8, Q8TBP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3