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Protein

Eukaryotic translation initiation factor 4 gamma 2

Gene

EIF4G2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.4 Publications

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • poly(A) RNA binding Source: UniProtKB
  • translation factor activity, RNA binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB

GO - Biological processi

  • cell cycle arrest Source: ProtInc
  • cell death Source: ProtInc
  • cellular macromolecule biosynthetic process Source: ParkinsonsUK-UCL
  • negative regulation of autophagy Source: ParkinsonsUK-UCL
  • positive regulation of cell growth Source: ParkinsonsUK-UCL
  • regulation of translational initiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor, Repressor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000110321-MONOMER.
ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4 gamma 2
Short name:
eIF-4-gamma 2
Short name:
eIF-4G 2
Short name:
eIF4G 2
Alternative name(s):
Death-associated protein 5
Short name:
DAP-5
p97
Gene namesi
Name:EIF4G2Imported
Synonyms:DAP51 Publication
ORF Names:OK/SW-cl.75
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:3297. EIF4G2.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cytosol Source: Reactome
  • eukaryotic translation initiation factor 4F complex Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi1982.
PharmGKBiPA27723.

Polymorphism and mutation databases

BioMutaiEIF4G2.
DMDMi30315906.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002133251 – 907Eukaryotic translation initiation factor 4 gamma 2Add BLAST907

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei11PhosphoserineCombined sources1
Modified residuei89PhosphothreonineCombined sources1
Modified residuei360Omega-N-methylarginineCombined sources1
Modified residuei395PhosphoserineCombined sources1
Modified residuei431N6-methyllysineCombined sources1
Modified residuei443PhosphoserineCombined sources1
Modified residuei505Omega-N-methylarginineCombined sources1
Modified residuei508PhosphothreonineCombined sources1
Modified residuei514PhosphothreonineCombined sources1
Modified residuei902PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation; hyperphosphorylated during mitosis.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiP78344.
MaxQBiP78344.
PaxDbiP78344.
PeptideAtlasiP78344.
PRIDEiP78344.

PTM databases

iPTMnetiP78344.
PhosphoSitePlusiP78344.
SwissPalmiP78344.

Miscellaneous databases

PMAP-CutDBP78344.

Expressioni

Tissue specificityi

Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.3 Publications

Gene expression databases

BgeeiENSG00000110321.
CleanExiHS_EIF4G2.
ExpressionAtlasiP78344. baseline and differential.
GenevisibleiP78344. HS.

Organism-specific databases

HPAiCAB005163.
HPA006773.
HPA016965.

Interactioni

Subunit structurei

Interacts with the serine/threonine protein kinases MKNK1 and MKNK2. Binds EIF4A and EIF3. Interacts with MIF4GD.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P299914EBI-296519,EBI-8826747From a different organism.
EIF2S2P200424EBI-296519,EBI-711977
EIF4A1P608423EBI-296519,EBI-73449
HSPB1P047923EBI-296519,EBI-352682

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi108297. 34 interactors.
DIPiDIP-31366N.
IntActiP78344. 24 interactors.
MINTiMINT-1182471.
STRINGi9606.ENSP00000340281.

Structurei

Secondary structure

1907
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi68 – 86Combined sources19
Turni90 – 92Combined sources3
Helixi93 – 103Combined sources11
Helixi108 – 124Combined sources17
Helixi126 – 128Combined sources3
Helixi129 – 142Combined sources14
Turni149 – 152Combined sources4
Helixi162 – 182Combined sources21
Beta strandi188 – 190Combined sources3
Helixi196 – 218Combined sources23
Turni219 – 221Combined sources3
Helixi225 – 236Combined sources12
Beta strandi240 – 242Combined sources3
Helixi244 – 246Combined sources3
Helixi248 – 265Combined sources18
Helixi268 – 270Combined sources3
Helixi271 – 282Combined sources12
Turni283 – 286Combined sources4
Helixi292 – 306Combined sources15
Turni307 – 309Combined sources3
Helixi541 – 558Combined sources18
Helixi561 – 571Combined sources11
Helixi575 – 577Combined sources3
Helixi578 – 590Combined sources13
Helixi594 – 609Combined sources16
Helixi615 – 627Combined sources13
Helixi629 – 635Combined sources7
Helixi639 – 652Combined sources14
Helixi658 – 665Combined sources8
Helixi666 – 668Combined sources3
Turni669 – 672Combined sources4
Helixi673 – 684Combined sources12
Helixi687 – 697Combined sources11
Helixi702 – 704Combined sources3
Helixi707 – 709Combined sources3
Helixi712 – 722Combined sources11
Helixi725 – 727Combined sources3
Helixi731 – 743Combined sources13
Helixi747 – 757Combined sources11
Helixi760 – 764Combined sources5
Helixi766 – 784Combined sources19
Helixi799 – 820Combined sources22
Helixi824 – 840Combined sources17
Helixi847 – 857Combined sources11
Helixi863 – 871Combined sources9
Helixi880 – 895Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D3MX-ray1.90A/B730-897[»]
3L6AX-ray2.00A540-897[»]
4IULX-ray2.30A/B61-323[»]
ProteinModelPortaliP78344.
SMRiP78344.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78344.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 308MIF4GPROSITE-ProRule annotationAdd BLAST231
Domaini543 – 666MIPROSITE-ProRule annotationAdd BLAST124
Domaini720 – 904W2PROSITE-ProRule annotationAdd BLAST185

Sequence similaritiesi

Contains 1 MI domain.PROSITE-ProRule annotation
Contains 1 MIF4G domain.Curated
Contains 1 W2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IPIB. Eukaryota.
ENOG410ZIZB. LUCA.
HOVERGENiHBG052084.
InParanoidiP78344.
KOiK03260.
OrthoDBiEOG091G03ZW.
PhylomeDBiP78344.

Family and domain databases

Gene3Di1.25.40.180. 3 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
IPR003307. W2_domain.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
PF02854. MIF4G. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
SM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS51366. MI. 1 hit.
PS51363. W2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P78344-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESAIAEGGA SRFSASSGGG GSRGAPQHYP KTAGNSEFLG KTPGQNAQKW
60 70 80 90 100
IPARSTRRDD NSAANNSANE KERHDAIFRK VRGILNKLTP EKFDKLCLEL
110 120 130 140 150
LNVGVESKLI LKGVILLIVD KALEEPKYSS LYAQLCLRLA EDAPNFDGPA
160 170 180 190 200
AEGQPGQKQS TTFRRLLISK LQDEFENRTR NVDVYDKREN PLLPEEEEQR
210 220 230 240 250
AIAKIKMLGN IKFIGELGKL DLIHESILHK CIKTLLEKKK RVQLKDMGED
260 270 280 290 300
LECLCQIMRT VGPRLDHERA KSLMDQYFAR MCSLMLSKEL PARIRFLLQD
310 320 330 340 350
TVELREHHWV PRKAFLDNGP KTINQIRQDA VKDLGVFIPA PMAQGMRSDF
360 370 380 390 400
FLEGPFMPPR MKMDRDPLGG LADMFGQMPG SGIGTGPGVI QDRFSPTMGR
410 420 430 440 450
HRSNQLFNGH GGHIMPPTQS QFGEMGGKFM KSQGLSQLYH NQSQGLLSQL
460 470 480 490 500
QGQSKDMPPR FSKKGQLNAD EISLRPAQSF LMNKNQVPKL QPQITMIPPS
510 520 530 540 550
AQPPRTQTPP LGQTPQLGLK TNPPLIQEKP AKTSKKPPPS KEELLKLTET
560 570 580 590 600
VVTEYLNSGN ANEAVNGVRE MRAPKHFLPE MLSKVIILSL DRSDEDKEKA
610 620 630 640 650
SSLISLLKQE GIATSDNFMQ AFLNVLDQCP KLEVDIPLVK SYLAQFAARA
660 670 680 690 700
IISELVSISE LAQPLESGTH FPLFLLCLQQ LAKLQDREWL TELFQQSKVN
710 720 730 740 750
MQKMLPEIDQ NKDRMLEILE GKGLSFLFPL LKLEKELLKQ IKLDPSPQTI
760 770 780 790 800
YKWIKDNISP KLHVDKGFVN ILMTSFLQYI SSEVNPPSDE TDSSSAPSKE
810 820 830 840 850
QLEQEKQLLL SFKPVMQKFL HDHVDLQVSA LYALQVHCYN SNFPKGMLLR
860 870 880 890 900
FFVHFYDMEI IEEEAFLAWK EDITQEFPGK GKALFQVNQW LTWLETAEEE

ESEEEAD
Length:907
Mass (Da):102,362
Last modified:May 1, 1997 - v1
Checksum:i4EF050B5EEA4DF91
GO
Isoform 2 (identifier: P78344-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-471: Missing.

Note: No experimental confirmation available.
Show »
Length:869
Mass (Da):98,150
Checksum:iF400E1E28654A2E4
GO

Sequence cautioni

The sequence BAB93515 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAD97268 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical GTG valine codon.Curated
The sequence CAA61857 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical GTG valine codon.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti169S → P in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti233K → Q in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti245K → Q in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti351F → L in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti441N → S in AAH18746 (PubMed:15489334).Curated1
Sequence conflicti531A → G in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti534S → N in AAH43149 (PubMed:15489334).Curated1
Sequence conflicti613A → G in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti861I → S in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti899E → G in AAC51166 (PubMed:9030685).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048923236L → M.Corresponds to variant rs34885591dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038726434 – 471Missing in isoform 2. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73824 mRNA. Translation: AAB49973.1.
U76111 mRNA. Translation: AAC51166.1.
X89713 mRNA. Translation: CAA61857.1. Sequence problems.
AK223548 mRNA. Translation: BAD97268.1. Sequence problems.
AC116535 Genomic DNA. No translation available.
BC014930 mRNA. Translation: AAH14930.2.
BC018746 mRNA. Translation: AAH18746.1.
BC018975 mRNA. Translation: AAH18975.1.
BC039851 mRNA. Translation: AAH39851.1.
BC043149 mRNA. Translation: AAH43149.2.
BC111415 mRNA. Translation: AAI11416.1.
BC111548 mRNA. Translation: AAI11549.2.
AB063323 mRNA. Translation: BAB93515.1. Different initiation.
CCDSiCCDS31428.1. [P78344-1]
CCDS41618.1. [P78344-2]
RefSeqiNP_001036024.3. NM_001042559.2. [P78344-2]
NP_001166176.1. NM_001172705.1. [P78344-1]
NP_001409.3. NM_001418.3. [P78344-1]
UniGeneiHs.183684.

Genome annotation databases

EnsembliENST00000339995; ENSP00000340281; ENSG00000110321.
GeneIDi1982.
KEGGihsa:1982.
UCSCiuc057zbi.1. human. [P78344-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73824 mRNA. Translation: AAB49973.1.
U76111 mRNA. Translation: AAC51166.1.
X89713 mRNA. Translation: CAA61857.1. Sequence problems.
AK223548 mRNA. Translation: BAD97268.1. Sequence problems.
AC116535 Genomic DNA. No translation available.
BC014930 mRNA. Translation: AAH14930.2.
BC018746 mRNA. Translation: AAH18746.1.
BC018975 mRNA. Translation: AAH18975.1.
BC039851 mRNA. Translation: AAH39851.1.
BC043149 mRNA. Translation: AAH43149.2.
BC111415 mRNA. Translation: AAI11416.1.
BC111548 mRNA. Translation: AAI11549.2.
AB063323 mRNA. Translation: BAB93515.1. Different initiation.
CCDSiCCDS31428.1. [P78344-1]
CCDS41618.1. [P78344-2]
RefSeqiNP_001036024.3. NM_001042559.2. [P78344-2]
NP_001166176.1. NM_001172705.1. [P78344-1]
NP_001409.3. NM_001418.3. [P78344-1]
UniGeneiHs.183684.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D3MX-ray1.90A/B730-897[»]
3L6AX-ray2.00A540-897[»]
4IULX-ray2.30A/B61-323[»]
ProteinModelPortaliP78344.
SMRiP78344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108297. 34 interactors.
DIPiDIP-31366N.
IntActiP78344. 24 interactors.
MINTiMINT-1182471.
STRINGi9606.ENSP00000340281.

PTM databases

iPTMnetiP78344.
PhosphoSitePlusiP78344.
SwissPalmiP78344.

Polymorphism and mutation databases

BioMutaiEIF4G2.
DMDMi30315906.

Proteomic databases

EPDiP78344.
MaxQBiP78344.
PaxDbiP78344.
PeptideAtlasiP78344.
PRIDEiP78344.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339995; ENSP00000340281; ENSG00000110321.
GeneIDi1982.
KEGGihsa:1982.
UCSCiuc057zbi.1. human. [P78344-1]

Organism-specific databases

CTDi1982.
DisGeNETi1982.
GeneCardsiEIF4G2.
HGNCiHGNC:3297. EIF4G2.
HPAiCAB005163.
HPA006773.
HPA016965.
MIMi602325. gene.
neXtProtiNX_P78344.
PharmGKBiPA27723.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPIB. Eukaryota.
ENOG410ZIZB. LUCA.
HOVERGENiHBG052084.
InParanoidiP78344.
KOiK03260.
OrthoDBiEOG091G03ZW.
PhylomeDBiP78344.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000110321-MONOMER.
ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.

Miscellaneous databases

ChiTaRSiEIF4G2. human.
EvolutionaryTraceiP78344.
GeneWikiiEIF4G2.
GenomeRNAii1982.
PMAP-CutDBP78344.
PROiP78344.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110321.
CleanExiHS_EIF4G2.
ExpressionAtlasiP78344. baseline and differential.
GenevisibleiP78344. HS.

Family and domain databases

Gene3Di1.25.40.180. 3 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
IPR003307. W2_domain.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
PF02854. MIF4G. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
SM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS51366. MI. 1 hit.
PS51363. W2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4G2_HUMAN
AccessioniPrimary (citable) accession number: P78344
Secondary accession number(s): O60877
, P78404, Q0VH00, Q0VH01, Q2NKW9, Q49A79, Q53EU1, Q58EZ2, Q8NI71, Q96C16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This gene has been shown to be extensively edited in the liver of APOBEC1 transgenic animal model. Its aberrant editing could contribute to the potent oncogenesis induced by overexpression of APOBEC1. The aberrant edited sequence, called NAT1, is likely to be a fundamental translational repressor.

Caution

According to PubMed:9049310, this sequence initiates exclusively at a GTG codon.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.