Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P78334

- GBRE_HUMAN

UniProt

P78334 - GBRE_HUMAN

Protein

Gamma-aminobutyric acid receptor subunit epsilon

Gene

GABRE

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (21 Feb 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

    GO - Molecular functioni

    1. chloride channel activity Source: UniProtKB-KW
    2. extracellular ligand-gated ion channel activity Source: InterPro
    3. GABA-A receptor activity Source: ProtInc

    GO - Biological processi

    1. chloride transport Source: InterPro
    2. gamma-aminobutyric acid signaling pathway Source: ProtInc
    3. transport Source: ProtInc

    Keywords - Molecular functioni

    Chloride channel, Ion channel

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Chloride

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gamma-aminobutyric acid receptor subunit epsilon
    Alternative name(s):
    GABA(A) receptor subunit epsilon
    Gene namesi
    Name:GABRE
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome X

    Organism-specific databases

    HGNCiHGNC:4085. GABRE.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. chloride channel complex Source: UniProtKB-KW
    3. integral component of membrane Source: UniProtKB-KW
    4. integral component of plasma membrane Source: ProtInc
    5. plasma membrane Source: UniProtKB-KW
    6. postsynaptic membrane Source: UniProtKB-SubCell
    7. synapse Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28499.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 506484Gamma-aminobutyric acid receptor subunit epsilonPRO_0000000471Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi195 ↔ 209By similarity
    Glycosylationi252 – 2521N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP78334.
    PRIDEiP78334.

    PTM databases

    PhosphoSiteiP78334.

    Expressioni

    Tissue specificityi

    Expressed in many tissues. Highest levels of expression in adult heart and placenta.

    Gene expression databases

    ArrayExpressiP78334.
    BgeeiP78334.
    CleanExiHS_GABRE.
    GenevestigatoriP78334.

    Organism-specific databases

    HPAiHPA045918.

    Interactioni

    Subunit structurei

    Generally pentameric. Associates with alpha and beta subunits.

    Protein-protein interaction databases

    STRINGi9606.ENSP00000359353.

    Structurei

    3D structure databases

    ProteinModelPortaliP78334.
    SMRiP78334. Positions 72-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 253231ExtracellularCuratedAdd
    BLAST
    Topological domaini365 – 485121CytoplasmicCuratedAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei254 – 27421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei281 – 30121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei344 – 36421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei486 – 50621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG259781.
    HOGENOMiHOG000231337.
    HOVERGENiHBG051707.
    InParanoidiP78334.
    KOiK05185.
    OMAiLYTIRMT.
    OrthoDBiEOG7JX342.
    PhylomeDBiP78334.
    TreeFamiTF315453.

    Family and domain databases

    Gene3Di2.70.170.10. 1 hit.
    InterProiIPR006028. GABAA/Glycine_rcpt.
    IPR008099. GABAAe_rcpt.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    [Graphical view]
    PANTHERiPTHR18945. PTHR18945. 1 hit.
    PfamiPF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view]
    PRINTSiPR00253. GABAARECEPTR.
    PR01723. GABAAREPSLON.
    PR00252. NRIONCHANNEL.
    SUPFAMiSSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 2 hits.
    TIGRFAMsiTIGR00860. LIC. 1 hit.
    PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P78334-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLSKVLPVLL GILLILQSRV EGPQTESKNE ASSRDVVYGP QPQPLENQLL    50
    SEETKSTETE TGSRVGKLPE ASRILNTILS NYDHKLRPGI GEKPTVVTVE 100
    ISVNSLGPLS ILDMEYTIDI IFSQTWYDER LCYNDTFESL VLNGNVVSQL 150
    WIPDTFFRNS KRTHEHEITM PNQMVRIYKD GKVLYTIRMT IDAGCSLHML 200
    RFPMDSHSCP LSFSSFSYPE NEMIYKWENF KLEINEKNSW KLFQFDFTGV 250
    SNKTEIITTP VGDFMVMTIF FNVSRRFGYV AFQNYVPSSV TTMLSWVSFW 300
    IKTESAPART SLGITSVLTM TTLGTFSRKN FPRVSYITAL DFYIAICFVF 350
    CFCALLEFAV LNFLIYNQTK AHASPKLRHP RINSRAHART RARSRACARQ 400
    HQEAFVCQIV TTEGSDGEER PSCSAQQPPS PGSPEGPRSL CSKLACCEWC 450
    KRFKKYFCMV PDCEGSTWQQ GRLCIHVYRL DNYSRVVFPV TFFFFNVLYW 500
    LVCLNL 506
    Length:506
    Mass (Da):57,972
    Last modified:February 21, 2001 - v2
    Checksum:i013EE136EECFCC73
    GO
    Isoform 2 (identifier: P78334-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         365-365: I → M
         366-506: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:365
    Mass (Da):41,760
    Checksum:iED38A831194D3EFD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti245 – 2451F → L in CAA70903. (PubMed:9339354)Curated
    Sequence conflicti261 – 2611Missing in CAA68914. (PubMed:9084408)Curated
    Sequence conflicti471 – 4711G → A in CAA70903. (PubMed:9339354)Curated
    Sequence conflicti502 – 5021V → F in CAA70903. (PubMed:9339354)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti9 – 91L → F.1 Publication
    Corresponds to variant rs17855708 [ dbSNP | Ensembl ].
    VAR_071089
    Natural varianti102 – 1021S → A.3 Publications
    Corresponds to variant rs1139916 [ dbSNP | Ensembl ].
    VAR_048175

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei365 – 3651I → M in isoform 2. 1 PublicationVSP_055776
    Alternative sequencei366 – 506141Missing in isoform 2. 1 PublicationVSP_055777Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U66661 mRNA. Translation: AAB49284.1.
    Y09763, Y09764 Genomic DNA. Translation: CAA70903.1.
    Y09765 mRNA. Translation: CAA70904.1.
    Y07637 mRNA. Translation: CAA68914.1.
    U92283, U92281, U92282 Genomic DNA. Translation: AAB94645.1.
    AF274855 Genomic DNA. No translation available.
    BC026337 mRNA. Translation: AAH26337.1.
    BC047108 mRNA. Translation: AAH47108.1.
    BC059376 mRNA. Translation: AAH59376.1.
    CCDSiCCDS14703.1.
    RefSeqiNP_004952.2. NM_004961.3.
    UniGeneiHs.22785.

    Genome annotation databases

    EnsembliENST00000370328; ENSP00000359353; ENSG00000102287. [P78334-1]
    GeneIDi2564.
    KEGGihsa:2564.
    UCSCiuc004ffi.3. human.

    Polymorphism databases

    DMDMi13124728.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U66661 mRNA. Translation: AAB49284.1 .
    Y09763 , Y09764 Genomic DNA. Translation: CAA70903.1 .
    Y09765 mRNA. Translation: CAA70904.1 .
    Y07637 mRNA. Translation: CAA68914.1 .
    U92283 , U92281 , U92282 Genomic DNA. Translation: AAB94645.1 .
    AF274855 Genomic DNA. No translation available.
    BC026337 mRNA. Translation: AAH26337.1 .
    BC047108 mRNA. Translation: AAH47108.1 .
    BC059376 mRNA. Translation: AAH59376.1 .
    CCDSi CCDS14703.1.
    RefSeqi NP_004952.2. NM_004961.3.
    UniGenei Hs.22785.

    3D structure databases

    ProteinModelPortali P78334.
    SMRi P78334. Positions 72-364.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000359353.

    Chemistry

    BindingDBi P78334.
    ChEMBLi CHEMBL2093872.
    DrugBanki DB00659. Acamprosate.
    DB00546. Adinazolam.
    DB00404. Alprazolam.
    DB00543. Amoxapine.
    DB01558. Bromazepam.
    DB00237. Butabarbital.
    DB00241. Butalbital.
    DB00475. Chlordiazepoxide.
    DB01594. Cinolazepam.
    DB00349. Clobazam.
    DB01068. Clonazepam.
    DB00628. Clorazepate.
    DB01559. Clotiazepam.
    DB01189. Desflurane.
    DB00829. Diazepam.
    DB00228. Enflurane.
    DB01049. Ergoloid mesylate.
    DB01215. Estazolam.
    DB00402. Eszopiclone.
    DB00189. Ethchlorvynol.
    DB00292. Etomidate.
    DB01567. Fludiazepam.
    DB01205. Flumazenil.
    DB00690. Flurazepam.
    DB01437. Glutethimide.
    DB00801. Halazepam.
    DB01159. Halothane.
    DB00753. Isoflurane.
    DB01587. Ketazolam.
    DB00186. Lorazepam.
    DB00371. Meprobamate.
    DB01028. Methoxyflurane.
    DB01107. Methyprylon.
    DB00683. Midazolam.
    DB01595. Nitrazepam.
    DB00334. Olanzapine.
    DB00842. Oxazepam.
    DB00312. Pentobarbital.
    DB01588. Prazepam.
    DB00794. Primidone.
    DB00818. Propofol.
    DB01589. Quazepam.
    DB01236. Sevoflurane.
    DB00306. Talbutal.
    DB00231. Temazepam.
    DB00273. Topiramate.
    DB00897. Triazolam.
    GuidetoPHARMACOLOGYi 417.

    PTM databases

    PhosphoSitei P78334.

    Polymorphism databases

    DMDMi 13124728.

    Proteomic databases

    PaxDbi P78334.
    PRIDEi P78334.

    Protocols and materials databases

    DNASUi 2564.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000370328 ; ENSP00000359353 ; ENSG00000102287 . [P78334-1 ]
    GeneIDi 2564.
    KEGGi hsa:2564.
    UCSCi uc004ffi.3. human.

    Organism-specific databases

    CTDi 2564.
    GeneCardsi GC0XM151121.
    HGNCi HGNC:4085. GABRE.
    HPAi HPA045918.
    MIMi 300093. gene.
    neXtProti NX_P78334.
    PharmGKBi PA28499.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG259781.
    HOGENOMi HOG000231337.
    HOVERGENi HBG051707.
    InParanoidi P78334.
    KOi K05185.
    OMAi LYTIRMT.
    OrthoDBi EOG7JX342.
    PhylomeDBi P78334.
    TreeFami TF315453.

    Miscellaneous databases

    ChiTaRSi GABRE. human.
    GeneWikii GABRE.
    GenomeRNAii 2564.
    NextBioi 10131.
    PROi P78334.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P78334.
    Bgeei P78334.
    CleanExi HS_GABRE.
    Genevestigatori P78334.

    Family and domain databases

    Gene3Di 2.70.170.10. 1 hit.
    InterProi IPR006028. GABAA/Glycine_rcpt.
    IPR008099. GABAAe_rcpt.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    [Graphical view ]
    PANTHERi PTHR18945. PTHR18945. 1 hit.
    Pfami PF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view ]
    PRINTSi PR00253. GABAARECEPTR.
    PR01723. GABAAREPSLON.
    PR00252. NRIONCHANNEL.
    SUPFAMi SSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 2 hits.
    TIGRFAMsi TIGR00860. LIC. 1 hit.
    PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Insensitivity to anaesthetic agents conferred by a class of GABA(A) receptor subunit."
      Davies P.A., Hanna M.C., Hales T.G., Kirkness E.F.
      Nature 385:820-823(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-102.
    2. "A gene in human chromosome band Xq28 (GABRE) defines a putative new subunit class of the GABAA neurotransmitter receptor."
      Wilke K., Gaul R., Klauck S.M., Poustka A.
      Genomics 45:1-10(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. "An mRNA encoding a putative GABA-gated chloride channel is expressed in the human cardiac conduction system."
      Garret M., Bascles L., Boue-Grabot E., Sartor P., Charron G., Bloch B., Margolskee R.F.
      J. Neurochem. 68:1382-1389(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-102.
    4. "Alternative transcripts of a gene encoding the GABA-A receptor epsilon subunit on chromosome Xq28."
      Hanna M.C., Hales T.G., Kirkness E.F.
      Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "The DNA sequence of the human X chromosome."
      Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
      , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
      Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS PHE-9 AND ALA-102.
      Tissue: Brain.

    Entry informationi

    Entry nameiGBRE_HUMAN
    AccessioniPrimary (citable) accession number: P78334
    Secondary accession number(s): E7ET93
    , O15345, O15346, Q6PCD2, Q99520
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: February 21, 2001
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3