P78332 (RBM6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-binding protein 6

Alternative name(s):
Lung cancer antigen NY-LU-12
Protein G16
RNA-binding motif protein 6
RNA-binding protein DEF-3

Gene names

Name:	RBM6
Synonyms:	DEF3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1123 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically binds poly(G) RNA homopolymers in vitro.
Subcellular location	Nucleus Probable.
Tissue specificity	Ubiquitous in adults.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13
Sequence similarities	Contains 1 G-patch domain. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA processing Traceable author statement. Source: ProtInc
Cellular component	nucleus Traceable author statement. Source: ProtInc
Molecular function	DNA binding Traceable author statement. Source: ProtInc RNA binding Traceable author statement. Source: ProtInc nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1123	1123	RNA-binding protein 6		PRO_0000081760
Regions
	Domain	456 – 536	81	RRM
	Domain	1051 – 1097	47	G-patch
	Compositional bias	826 – 829	4	Poly-Glu
	Compositional bias	892 – 895	4	Poly-Pro
	Compositional bias	915 – 921	7	Poly-Glu
Amino acid modifications
	Modified residue	360	1	Phosphoserine Ref.9 Ref.11 Ref.13
	Modified residue	362	1	Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12 Ref.13
	Modified residue	891	1	Phosphoserine Ref.7 Ref.10
	Modified residue	914	1	Phosphotyrosine Ref.13
	Modified residue	923	1	Phosphothreonine Ref.6
	Modified residue	1022	1	Phosphoserine Ref.10
	Modified residue	1025	1	Phosphoserine Ref.7 Ref.8 Ref.10 Ref.11
Natural variations
	Natural variant	353	1	S → F in a non-small cell lung cancer cell line. Ref.5		VAR_014226
	Natural variant	721	1	N → T. Corresponds to variant rs34707170 [ dbSNP | Ensembl ].		VAR_052216
Experimental info
	Sequence conflict	642	1	R → K in AAC21578. Ref.2
	Sequence conflict	710	1	A → V in AAC05826. Ref.1
	Sequence conflict	796	1	T → S in AAC05826. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P78332 [UniParc]. Last modified October 17, 2006. Version 5. Checksum: 2952ED1BAA839DE4	FASTA	1,123	128,644
	10 20 30 40 50 60 MWGDSRPANR TGPFRGSQEE RFAPGWNRDY PPPPLKSHAQ ERHSGNFPGR DSLPFDFQGH 70 80 90 100 110 120 SGPPFANVEE HSFSYGARDG PHGDYRGGEG PGHDFRGGDF SSSDFQSRDS SQLDFRGRDI 130 140 150 160 170 180 HSGDFRDREG PPMDYRGGDG TSMDYRGREA PHMNYRDRDA HAVDFRGRDA PPSDFRGRGT 190 200 210 220 230 240 YDLDFRGRDG SHADFRGRDL SDLDFRAREQ SRSDFRNRDV SDLDFRDKDG TQVDFRGRGS 250 260 270 280 290 300 GTTDLDFRDR DTPHSDFRGR HRSRTDQDFR GREMGSCMEF KDREMPPVDP NILDYIQPST 310 320 330 340 350 360 QDREHSGMNV NRREESTHDH TIERPAFGIQ KGEFEHSETR EGETQGVAFE HESPADFQNS 370 380 390 400 410 420 QSPVQDQDKS QLSGREEQSS DAGLFKEEGG LDFLGRQDTD YRSMEYRDVD HRLPGSQMFG 430 440 450 460 470 480 YGQSKSFPEG KTARDAQRDL QDQDYRTGPS EEKPSRLIRL SGVPEDATKE EILNAFRTPD 490 500 510 520 530 540 GMPVKNLQLK EYNTGYDYGY VCVEFSLLED AIGCMEANQG TLMIQDKEVT LEYVSSLDFW 550 560 570 580 590 600 YCKRCKANIG GHRSSCSFCK NPREVTEAKQ ELITYPQPQK TSIPAPLEKQ PNQPLRPADK 610 620 630 640 650 660 EPEPRKREEG QESRLGHQKR EAERYLPPSR REGPTFRRDR ERESWSGETR QDGESKTIML 670 680 690 700 710 720 KRIYRSTPPE VIVEVLEPYV RLTTANVRII KNRTGPMGHT YGFIDLDSHA EALRVVKILQ 730 740 750 760 770 780 NLDPPFSIDG KMVAVNLATG KRRNDSGDHS DHMHYYQGKK YFRDRRGGGR NSDWSSDTNR 790 800 810 820 830 840 QGQQSSSDCY IYDSATGYYY DPLAGTYYDP NTQQEVYVPQ DPGLPEEEEI KEKKPTSQGK 850 860 870 880 890 900 SSSKKEMSKR DGKEKKDRGV TRFQENASEG KAPAEDVFKK PLPPTVKKEE SPPPPKVVNP 910 920 930 940 950 960 LIGLLGEYGG DSDYEEEEEE EQTPPPQPRT AQPQKREEQT KKENEEDKLT DWNKLACLLC 970 980 990 1000 1010 1020 RRQFPNKEVL IKHQQLSDLH KQNLEIHRKI KQSEQELAYL ERREREGKFK GRGNDRREKL 1030 1040 1050 1060 1070 1080 QSFDSPERKR IKYSRETDSD RKLVDKEDID TSSKGGCVQQ ATGWRKGTGL GYGHPGLASS 1090 1100 1110 1120 EEAEGRMRGP SVGASGRTSK RQSNETYRDA VRRVMFARYK ELD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human lung cancer antigens recognized by autologous antibodies: definition of a novel cDNA derived from the tumor suppressor gene locus on chromosome 3p21.3." Gure A.O., Altorki N.K., Stockert E., Scanlan M.J., Old L.J., Chen Y.-T. Cancer Res. 58:1034-1041(1998) [PubMed: 9500467] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"A comparison of genomic structures and expression patterns of two closely related flanking genes in a critical lung cancer region at 3p21.3." Timmer T., Terpstra P., van den Berg A., Veldhuis P.M., Ter Elst A., Voutsinas G., Hulsbeek M.M.F., Draaijers T.G., Looman M.W.G., Kok K., Naylor S.L., Buys C.H.C.M. Eur. J. Hum. Genet. 7:478-486(1999) [PubMed: 10352938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"DEF-3(g16/NY-LU-12), an RNA binding protein from the 3p21.3 homozygous deletion region in SCLC." Drabkin H.A., West J.D., Hotfilder M., Heng Y.M., Erickson P., Calvo R., Dalmau J., Gemmill R.M., Sablitzky F. Oncogene 18:2589-2597(1999) [PubMed: 10353602] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"A highly conserved and universally expressed gene is interrupted by a homozygous deletion in a small cell lung cancer cell line NCI-H740." Latif F., Duh F.-M., Wei M.H., Sekido Y., Forgacs E., Minna J.D., Lerman M.I. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"The 630-kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes." The international lung cancer chromosome 3p21.3 tumor suppressor gene consortium Lerman M.I., Minna J.D. Cancer Res. 60:6116-6133(2000) [PubMed: 11085536] [Abstract] Cited for: DISCUSSION OF SEQUENCE, VARIANT PHE-353.
[6]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-923, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-891 AND SER-1025, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1025, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360 AND SER-362, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[10]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362; SER-891; SER-1022 AND SER-1025, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360; SER-362 AND SER-1025, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362, MASS SPECTROMETRY.
[13]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360; SER-362 AND TYR-914, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF042857 mRNA. Translation: AAC05826.1. AF069517 mRNA. Translation: AAC21578.1. AF091264 mRNA. Translation: AAD04160.1. U50839 mRNA. Translation: AAC35207.1.
IPI	IPI00297723.
RefSeq	NP_005768.1. NM_005777.2.
UniGene	Hs.696735.
3D structure databases
ProteinModelPortal	P78332.
SMR	P78332. Positions 454-564, 653-747.
ModBase	Search...
Protein-protein interaction databases
IntAct	P78332. 5 interactions.
STRING	P78332.
PTM databases
PhosphoSite	P78332.
Polymorphism databases
DMDM	116242749.
Proteomic databases
PRIDE	P78332.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000266022; ENSP00000266022; ENSG00000004534.
GeneID	10180.
KEGG	hsa:10180.
UCSC	uc003cyc.1. human.
Organism-specific databases
CTD	10180.
GeneCards	GC03P049952.
H-InvDB	HIX0003322.
HGNC	HGNC:9903. RBM6.
HPA	HPA026272. HPA027164.
MIM	606886. gene.
neXtProt	NX_P78332.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12886.
HOGENOM	HBG278020.
HOVERGEN	HBG007537.
InParanoid	P78332.
OMA	ERFAPGW.
PhylomeDB	P78332.
Gene expression databases
ArrayExpress	P78332.
Bgee	P78332.
CleanEx	HS_RBM6.
Genevestigator	P78332.
GermOnline	ENSG00000004534. Homo sapiens.
Family and domain databases
InterPro	IPR000467. G_patch. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR015880. Znf_C2H2-like. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
Pfam	PF01585. G-patch. 1 hit. [Graphical view]
SMART	SM00443. G_patch. 1 hit. SM00360. RRM. 1 hit. SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS50174. G_PATCH. 1 hit. PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	38538.
SOURCE	Search...

Entry information

Entry name

RBM6_HUMAN

Accession

Primary (citable) accession number: P78332
Secondary accession number(s): O60549, O75524

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	October 17, 2006
Last modified:	December 14, 2011
This is version 107 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.