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Reviewed, UniProtKB/Swiss-Prot P78329 (CP4F2_HUMAN)

Last modified November 25, 2008. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cytochrome P450 4F2
    EC=1.14.13.30
Alternative name(s):
    CYPIVF2
    Leukotriene-B(4) omega-hydroxylase
    Leukotriene-B(4) 20-monooxygenase
    Cytochrome P450-LTB-omega
Gene names
Name: CYP4F2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O(2) = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP(+) + H(2)O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Liver.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 520520Cytochrome P450 4F2
PRO_0000051850

Sites

Metal binding4681Iron (heme axial ligand) By similarity
Binding site3281Heme (covalent; via 1 link) By similarity

Natural variations

Natural variant71S → Y: dbSNP rs3093104.
VAR_013116
Natural variant121W → G: dbSNP rs3093105.
VAR_013117
Natural variant1851G → V: dbSNP rs3093153.
VAR_013118
Natural variant2691A → D: dbSNP rs1805040.
VAR_020125
Natural variant4331V → M: dbSNP rs2108622.
VAR_013119
Natural variant5191L → M: dbSNP rs3093200.
VAR_013120

Experimental info

Sequence conflict12 – 132WP → CR Ref.2 Ref.7
Sequence conflict251V → A in AAH67437. Ref.6
Sequence conflict1691E → D in AAH67440. Ref.6
Sequence conflict3361G → V in AAC50052. Ref.2
Sequence conflict3911L → V in AAC50052. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P78329-1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 1791F9E6EECB59B5

FASTA52059,853
        10         20         30         40         50         60 
MSQLSLSWLG LWPVAASPWL LLLLVGASWL LAHVLAWTYA FYDNCRRLRC FPQPPRRNWF 

        70         80         90        100        110        120 
WGHQGMVNPT EEGMRVLTQL VATYPQGFKV WMGPISPLLS LCHPDIIRSV INASAAIAPK 

       130        140        150        160        170        180 
DKFFYSFLEP WLGDGLLLSA GDKWSRHRRM LTPAFHFNIL KPYMKIFNES VNIMHAKWQL 

       190        200        210        220        230        240 
LASEGSACLD MFEHISLMTL DSLQKCVFSF DSHCQEKPSE YIAAILELSA LVSKRHHEIL 

       250        260        270        280        290        300 
LHIDFLYYLT PDGQRFRRAC RLVHDFTDAV IQERRRTLPS QGVDDFLQAK AKSKTLDFID 

       310        320        330        340        350        360 
VLLLSKDEDG KKLSDEDIRA EADTFMFEGH DTTASGLSWV LYHLAKHPEY QERCRQEVQE 

       370        380        390        400        410        420 
LLKDREPKEI EWDDLAHLPF LTMCMKESLR LHPPVPVISR HVTQDIVLPD GRVIPKGIIC 

       430        440        450        460        470        480 
LISVFGTHHN PAVWPDPEVY DPFRFDPENI KERSPLAFIP FSAGPRNCIG QTFAMAEMKV 

       490        500        510        520 
VLALTLLRFR VLPDHTEPRR KPELVLRAEG GLWLRVEPLS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of a novel form of leukotriene B4 omega-hydroxylase from human liver."
Kikuta Y., Kusunose E., Kondo T., Yamamoto S., Kinoshita H., Kusunose M.
FEBS Lett. 348:70-74(1994) [PubMed: 8026587] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"The human liver CYP4F2 cDNA sequence and expression in baculovirus-infected insect cells."
Chen L., Hardwick J.P.
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"Expression and molecular cloning of human liver leukotriene B4 omega-hydroxylase (CYP4F2) gene."
Kikuta Y., Miyauchi Y., Kusunose E., Kusunose M.
DNA Cell Biol. 18:723-730(1999) [PubMed: 10492403] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]SeattleSNPs program for genomic applications
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS TYR-7; GLY-12; VAL-185; MET-433 AND MET-519.
[5]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed: 15057824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLY-12 AND MET-433.
[7]"Promoter activity and regulation of the CYP4F2 leukotriene B4 omega hydroxylase gene by peroxisomal proliferators and retinoic acid in HepG2 cells."
Zhang X., Chen L., Hardwick J.P.
Arch. Biochem. Biophys. 378:364-376(2000) [PubMed: 10860554] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-175.
Tissue: Liver.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

D26480 mRNA. Translation: BAA05490.1.
U02388 mRNA. Translation: AAC50052.2.
AB015306 Genomic DNA. Translation: BAA75823.1.
AF467894 Genomic DNA. Translation: AAL67578.1.
AC005336 Genomic DNA. Translation: AAC27730.1.
BC067437 mRNA. Translation: AAH67437.1.
BC067439 mRNA. Translation: AAH67439.1.
BC067440 mRNA. Translation: AAH67440.1.
AF221943 Genomic DNA. Translation: AAF86378.1.
PIRS45702.
RefSeqNP_001073.3.
UniGeneHs.558423

3D structure databases

HSSPHSSP built from PDB template 1JME based on UniProtKB P14779.
ModBaseSearch...

Protein-protein interaction databases

IntActP78329.

PTM databases

PhosphoSiteP78329.

Genome annotation databases

EnsemblENSG00000186115. Homo sapiens. [Contig view]
GeneID8529.
KEGGhsa:8529.

Organism-specific databases

H-InvDBHIX0014854.
HIX0040119.
HGNCHGNC:2645. CYP4F2.
HPAHPA014048.
MIM604426. gene.
PharmGKBPA27121.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP78329.
HOVERGENP78329.

Gene expression databases

ArrayExpressP78329.
CleanExHS_CYP4F2.
GermOnlineENSG00000186115. Homo sapiens.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubP78329.
NextBio31940.
SOURCESearch...

Entry information

Entry nameCP4F2_HUMAN
AccessionPrimary (citable) accession number: P78329
Secondary accession number(s): Q16677 expand/collapse secondary AC list , Q6NWT4, Q6NWT6, Q9NNZ0, Q9UIU8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: November 25, 2008
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents