Reviewed, UniProtKB/Swiss-Prot P78329 (CP4F2_HUMAN)

Names and origin

Protein names

Recommended name:
    Leukotriene-B(4) omega-hydroxylase 1
    EC=1.14.13.30
Alternative name(s):
    Cytochrome P450 4F2
    CYPIVF2
    Leukotriene-B(4) 20-monooxygenase 1
    Cytochrome P450-LTB-omega

Gene names

Name:

CYP4F2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leukotriene metabolic process Ref.1 Traceable author statement. Source: ProtInc oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro leukotriene-B4 20-monooxygenase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABL1	P00519	1	EBI-1752413,EBI-375543
FYN	P06241	1	EBI-1752413,EBI-515315
GRB2	P62993	1	EBI-1752413,EBI-401755
NCK1	P16333	1	EBI-1752413,EBI-389883
SRC	P12931	1	EBI-1752413,EBI-621482

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Leukotriene-B(4) omega-hydroxylase 1		PRO_0000051850
Sites
	Metal binding	468	1	Iron (heme axial ligand) By similarity
	Binding site	328	1	Heme (covalent; via 1 link) By similarity
Natural variations
	Natural variant	7	1	S → Y: dbSNP rs3093104. Ref.5		VAR_013116
	Natural variant	12	1	W → G: dbSNP rs3093105. Ref.5 Ref.7		VAR_013117
	Natural variant	185	1	G → V: dbSNP rs3093153. Ref.5 Ref.4		VAR_013118
	Natural variant	269	1	A → D: dbSNP rs1805040.		VAR_020125
	Natural variant	433	1	V → M: dbSNP rs2108622. Ref.5 Ref.7		VAR_013119
	Natural variant	519	1	L → M: dbSNP rs3093200. Ref.5		VAR_013120
Experimental info
	Sequence conflict	12 – 13	2	WP → CR Ref.2
	Sequence conflict	12 – 13	2	WP → CR Ref.8
	Sequence conflict	25	1	V → A in AAH67437. Ref.7
	Sequence conflict	169	1	E → D in AAH67440. Ref.7
	Sequence conflict	336	1	G → V in AAC50052. Ref.2
	Sequence conflict	391	1	L → V in AAC50052. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P78329-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 1791F9E6EECB59B5	FASTA	520	59,853
	10 20 30 40 50 60 MSQLSLSWLG LWPVAASPWL LLLLVGASWL LAHVLAWTYA FYDNCRRLRC FPQPPRRNWF 70 80 90 100 110 120 WGHQGMVNPT EEGMRVLTQL VATYPQGFKV WMGPISPLLS LCHPDIIRSV INASAAIAPK 130 140 150 160 170 180 DKFFYSFLEP WLGDGLLLSA GDKWSRHRRM LTPAFHFNIL KPYMKIFNES VNIMHAKWQL 190 200 210 220 230 240 LASEGSACLD MFEHISLMTL DSLQKCVFSF DSHCQEKPSE YIAAILELSA LVSKRHHEIL 250 260 270 280 290 300 LHIDFLYYLT PDGQRFRRAC RLVHDFTDAV IQERRRTLPS QGVDDFLQAK AKSKTLDFID 310 320 330 340 350 360 VLLLSKDEDG KKLSDEDIRA EADTFMFEGH DTTASGLSWV LYHLAKHPEY QERCRQEVQE 370 380 390 400 410 420 LLKDREPKEI EWDDLAHLPF LTMCMKESLR LHPPVPVISR HVTQDIVLPD GRVIPKGIIC 430 440 450 460 470 480 LISVFGTHHN PAVWPDPEVY DPFRFDPENI KERSPLAFIP FSAGPRNCIG QTFAMAEMKV 490 500 510 520 VLALTLLRFR VLPDHTEPRR KPELVLRAEG GLWLRVEPLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a novel form of leukotriene B4 omega-hydroxylase from human liver." Kikuta Y., Kusunose E., Kondo T., Yamamoto S., Kinoshita H., Kusunose M. FEBS Lett. 348:70-74(1994) [PubMed: 8026587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The human liver CYP4F2 cDNA sequence and expression in baculovirus-infected insect cells." Chen L., Hardwick J.P. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Expression and molecular cloning of human liver leukotriene B4 omega-hydroxylase (CYP4F2) gene." Kikuta Y., Miyauchi Y., Kusunose E., Kusunose M. DNA Cell Biol. 18:723-730(1999) [PubMed: 10492403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-185. Tissue: Kidney.
[5]	SeattleSNPs variation discovery resource Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS TYR-7; GLY-12; VAL-185; MET-433 AND MET-519.
[6]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLY-12 AND MET-433.
[8]	"Promoter activity and regulation of the CYP4F2 leukotriene B4 omega hydroxylase gene by peroxisomal proliferators and retinoic acid in HepG2 cells." Zhang X., Chen L., Hardwick J.P. Arch. Biochem. Biophys. 378:364-376(2000) [PubMed: 10860554] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-175. Tissue: Liver.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	D26480 mRNA. Translation: BAA05490.1. U02388 mRNA. Translation: AAC50052.2. AB015306 Genomic DNA. Translation: BAA75823.1. AK290790 mRNA. Translation: BAF83479.1. AF467894 Genomic DNA. Translation: AAL67578.1. AC005336 Genomic DNA. Translation: AAC27730.1. BC067437 mRNA. Translation: AAH67437.1. BC067439 mRNA. Translation: AAH67439.1. BC067440 mRNA. Translation: AAH67440.1. AF221943 Genomic DNA. Translation: AAF86378.1.
IPI	IPI00019160.
PIR	S45702.
RefSeq	NP_001073.3.
UniGene	Hs.558423
3D structure databases
HSSP	HSSP built from PDB template 1JME based on UniProtKB P14779.
ModBase	Search...
Protein-protein interaction databases
IntAct	P78329. 5 interactions.
Proteomic databases
PRIDE	P78329.
Genome annotation databases
Ensembl	ENSG00000186115. Homo sapiens. [Contig view]
GeneID	8529.
KEGG	hsa:8529.
UCSC	uc002nbs.1. human.
Organism-specific databases
GeneCards	GC19M015849.
H-InvDB	HIX0014854. HIX0040119.
HGNC	HGNC:2645. CYP4F2.
HPA	HPA014048. HPA017265.
MIM	604426. gene.
PharmGKB	PA27121.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P78329.
HOVERGEN	P78329.
Enzyme and pathway databases
BRENDA	1.14.13.30. 247.
Reactome	REACT_13433. Biological oxidations.
Gene expression databases
ArrayExpress	P78329.
Bgee	P78329.
CleanEx	HS_CYP4F2.
GermOnline	ENSG00000186115. Homo sapiens.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	31940.
SOURCE	Search...

Entry information

Entry name

CP4F2_HUMAN

Accession

Primary (citable) accession number: P78329
Secondary accession number(s): A8K425 , Q16677, Q6NWT4, Q6NWT6, Q9NNZ0, Q9UIU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	July 7, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)