Disintegrin and metalloproteinase domain-containing protein 8 precursor

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P78325 (ADAM8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (7) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Disintegrin and metalloproteinase domain-containing protein 8
Short name=ADAM 8
EC=3.4.24.-

Alternative name(s):
Cell surface antigen MS2
CD_antigen=CD156a

Gene names

Name:	ADAM8
Synonyms:	MS2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible involvement in extravasation of leukocytes.
Cofactor	Binds 1 zinc ion per subunit Probable.
Subunit structure	Interacts with FST3. Ref.2
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed on neutrophils and monocytes.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	EGF-like domain Signal Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of MAPK activity involved in innate immune response Inferred from sequence or structural similarity. Source: BHF-UCL angiogenesis Inferred from sequence or structural similarity. Source: BHF-UCL cell morphogenesis Inferred from sequence or structural similarity. Source: BHF-UCL cellular response to hypoxia Inferred from direct assay. Source: BHF-UCL leukocyte migration involved in inflammatory response Inferred from sequence or structural similarity. Source: BHF-UCL lymphocyte chemotaxis Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of neuron apoptosis Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of NF-kappaB transcription factor activity Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of T cell differentiation in thymus Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of acute inflammatory response Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of bone resorption Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of cell adhesion Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of cellular extravasation Inferred from direct assay. Source: BHF-UCL positive regulation of membrane protein ectodomain proteolysis Inferred from direct assay. Source: BHF-UCL positive regulation of protein kinase B signaling cascade Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of protein processing Non-traceable author statement. Source: BHF-UCL positive regulation of protein secretion Inferred by curator. Source: BHF-UCL positive regulation of thymocyte apoptosis Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of tumor necrosis factor (ligand) superfamily member 11 production Inferred from sequence or structural similarity. Source: BHF-UCL proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell-cell adhesion Inferred from direct assay. Source: BHF-UCL
Cellular component	alpha9-beta1 integrin-ADAM8 complex Inferred from sequence or structural similarity. Source: BHF-UCL cell surface Inferred from direct assay. Source: BHF-UCL dense core granule membrane Inferred from direct assay. Source: BHF-UCL integral to plasma membrane Inferred from direct assay. Source: BHF-UCL phagolysosome Inferred from direct assay. Source: BHF-UCL podosome Inferred from direct assay. Source: BHF-UCL specific granule Inferred from direct assay. Source: BHF-UCL tertiary granule Inferred from direct assay. Source: BHF-UCL
Molecular function	calcium ion binding Inferred from sequence or structural similarity. Source: BHF-UCL cell adhesion molecule binding Inferred from physical interaction. Source: BHF-UCL metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro protein self-association Traceable author statement. Source: BHF-UCL zinc ion binding Traceable author statement. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

Potential

Chain

17 – 824

808

Disintegrin and metalloproteinase domain-containing protein 8

PRO_0000029060

Regions

Topological domain

17 – 655

639

Extracellular Potential

Transmembrane

656 – 676

Helical; Potential

Topological domain

677 – 824

148

Cytoplasmic Potential

Domain

200 – 400

201

Peptidase M12B

Domain

408 – 494

Disintegrin

Sites

Active site

335

By similarity

Metal binding

334

Zinc; catalytic Probable

Metal binding

338

Zinc; catalytic Probable

Metal binding

344

Zinc; catalytic Probable

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

436

N-linked (GlcNAc...) Potential

Glycosylation

612

N-linked (GlcNAc...) Potential

Disulfide bond

310 ↔ 395

By similarity

Disulfide bond

351 ↔ 379

By similarity

Disulfide bond

353 ↔ 362

By similarity

Disulfide bond

466 ↔ 486

By similarity

Disulfide bond

613 ↔ 623

By similarity

Disulfide bond

617 ↔ 629

By similarity

Disulfide bond

631 ↔ 640

By similarity

Natural variations

Natural variant

101

G → R.
Corresponds to variant rs11101675 [ dbSNP | Ensembl ].

VAR_059760

Natural variant

189

R → W.
Corresponds to variant rs45451297 [ dbSNP | Ensembl ].

VAR_061735

Natural variant

433

R → C.
Corresponds to variant rs12257830 [ dbSNP | Ensembl ].

VAR_061736

Natural variant

775

I → T.
Corresponds to variant rs3008319 [ dbSNP | Ensembl ].

VAR_061737

Sequences

Sequence

Length

Mass (Da)

Tools

P78325 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 5DF8E05F30DF479E
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FASTA

824

88,673

        10         20         30         40         50         60 
MRGLGLWLLG AMMLPAIAPS RPWALMEQYE VVLPRRLPGP RVRRALPSHL GLHPERVSYV 

        70         80         90        100        110        120 
LGATGHNFTL HLRKNRDLLG SGYTETYTAA NGSEVTEQPR GQDHCLYQGH VEGYPDSAAS 

       130        140        150        160        170        180 
LSTCAGLRGF FQVGSDLHLI EPLDEGGEGG RHAVYQAEHL LQTAGTCGVS DDSLGSLLGP 

       190        200        210        220        230        240 
RTAAVFRPRP GDSLPSRETR YVELYVVVDN AEFQMLGSEA AVRHRVLEVV NHVDKLYQKL 

       250        260        270        280        290        300 
NFRVVLVGLE IWNSQDRFHV SPDPSVTLEN LLTWQARQRT RRHLHDNVQL ITGVDFTGTT 

       310        320        330        340        350        360 
VGFARVSAMC SHSSGAVNQD HSKNPVGVAC TMAHEMGHNL GMDHDENVQG CRCQERFEAG 

       370        380        390        400        410        420 
RCIMAGSIGS SFPRMFSDCS QAYLESFLER PQSVCLANAP DLSHLVGGPV CGNLFVERGE 

       430        440        450        460        470        480 
QCDCGPPEDC RNRCCNSTTC QLAEGAQCAH GTCCQECKVK PAGELCRPKK DMCDLEEFCD 

       490        500        510        520        530        540 
GRHPECPEDA FQENGTPCSG GYCYNGACPT LAQQCQAFWG PGGQAAEESC FSYDILPGCK 

       550        560        570        580        590        600 
ASRYRADMCG VLQCKGGQQP LGRAICIVDV CHALTTEDGT AYEPVPEGTR CGPEKVCWKG 

       610        620        630        640        650        660 
RCQDLHVYRS SNCSAQCHNH GVCNHKQECH CHAGWAPPHC AKLLTEVHAA SGSLPVLVVV 

       670        680        690        700        710        720 
VLVLLAVVLV TLAGIIVYRK ARSRILSRNV APKTTMGRSN PLFHQAASRV PAKGGAPAPS 

       730        740        750        760        770        780 
RGPQELVPTT HPGQPARHPA SSVALKRPPP APPVTVSSPP FPVPVYTRQA PKQVIKPTFA 

       790        800        810        820 
PPVPPVKPGA GAANPGPAEG AVGPKVALKP PIQRKQGAGA PTAP

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References

[1]	"CD156 (human ADAM8): expression, primary amino acid sequence, and gene location." Yoshiyama K., Higuchi Y., Kataoka M., Matsuura K., Yamamoto S. Genomics 41:56-62(1997) [PubMed: 9126482] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Blood.
[2]	"FLRG, a new ADAM12-associated protein, modulates osteoclast differentiation." Bartholin L., Destaing O., Forissier S., Martel S., Maguer-Satta V., Jurdic P., Rimokh R. Biol. Cell 97:577-588(2005) [PubMed: 15574124] [Abstract] Cited for: INTERACTION WITH FST3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26579 mRNA. Translation: BAA05626.1.
IPI	IPI00019158.
UniGene	Hs.501574.
3D structure databases
ProteinModelPortal	P78325.
SMR	P78325. Positions 195-646.
ModBase	Search...
Protein-protein interaction databases
IntAct	P78325. 2 interactions.
STRING	P78325.
Protein family/group databases
MEROPS	M12.208.
PTM databases
PhosphoSite	P78325.
Polymorphism databases
DMDM	2499914.
Proteomic databases
PRIDE	P78325.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
GeneCards	GC10M135075.
H-InvDB	HIX0035404.
HGNC	HGNC:215. ADAM8.
MIM	602267. gene.
neXtProt	NX_P78325.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15149.
HOVERGEN	HBG006978.
InParanoid	P78325.
Gene expression databases
ArrayExpress	P78325.
CleanEx	HS_ADAM8.
Genevestigator	P78325.
GermOnline	ENSG00000151651. Homo sapiens.
Family and domain databases
InterPro	IPR006586. ADAM_Cys-rich. IPR001762. Blood-coag_inhib_Disintegrin. IPR018358. Disintegrin_CS. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR024079. MetalloPept_cat_dom. IPR001590. Peptidase_M12B. IPR002870. Peptidase_M12B_N. [Graphical view]
Gene3D	G3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit. G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
Pfam	PF08516. ADAM_CR. 1 hit. PF00200. Disintegrin. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. [Graphical view]
PRINTS	PR00289. DISINTEGRIN.
SMART	SM00608. ACR. 1 hit. SM00050. DISIN. 1 hit. SM00181. EGF. 1 hit. [Graphical view]
SUPFAM	SSF57552. Disintegrin. 1 hit.
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00427. DISINTEGRIN_1. 1 hit. PS50214. DISINTEGRIN_2. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	381.
SOURCE	Search...

Entry information

Entry name

ADAM8_HUMAN

Accession

Primary (citable) accession number: P78325

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.