Disintegrin and metalloproteinase domain-containing protein 8 precursor

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Reviewed, UniProtKB/Swiss-Prot P78325 (ADAM8_HUMAN)

Last modified November 24, 2009. Version 96. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 8
      Short name=ADAM 8
    EC=3.4.24.-
Alternative name(s):
    Cell surface antigen MS2
    CD_antigen=CD156a

Gene names

Name:	ADAM8
Synonyms:	MS2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Possible involvement in extravasation of leukocytes.
Cofactor	Binds 1 zinc ion per subunit Probable.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed on neutrophils and monocytes.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain.

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Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	EGF-like domain Signal Transmembrane
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

Potential

Chain

17 – 824

808

Disintegrin and metalloproteinase domain-containing protein 8

PRO_0000029060

Regions

Topological domain

17 – 655

639

Extracellular Potential

Transmembrane

656 – 676

Potential

Topological domain

677 – 824

148

Cytoplasmic Potential

Domain

200 – 400

201

Peptidase M12B

Domain

408 – 494

Disintegrin

Sites

Active site

335

By similarity

Metal binding

334

Zinc; catalytic Probable

Metal binding

338

Zinc; catalytic Probable

Metal binding

344

Zinc; catalytic Probable

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

436

N-linked (GlcNAc...) Potential

Glycosylation

612

N-linked (GlcNAc...) Potential

Disulfide bond

310 ↔ 395

By similarity

Disulfide bond

351 ↔ 379

By similarity

Disulfide bond

353 ↔ 362

By similarity

Disulfide bond

466 ↔ 486

By similarity

Disulfide bond

613 ↔ 623

By similarity

Disulfide bond

617 ↔ 629

By similarity

Disulfide bond

631 ↔ 640

By similarity

Natural variations

Natural variant

101

G → R: dbSNP rs11101675.

VAR_059760

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Sequences

Sequence

Length

Mass (Da)

Tools

P78325-1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 5DF8E05F30DF479E
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FASTA

824

88,673

        10         20         30         40         50         60 
MRGLGLWLLG AMMLPAIAPS RPWALMEQYE VVLPRRLPGP RVRRALPSHL GLHPERVSYV 

        70         80         90        100        110        120 
LGATGHNFTL HLRKNRDLLG SGYTETYTAA NGSEVTEQPR GQDHCLYQGH VEGYPDSAAS 

       130        140        150        160        170        180 
LSTCAGLRGF FQVGSDLHLI EPLDEGGEGG RHAVYQAEHL LQTAGTCGVS DDSLGSLLGP 

       190        200        210        220        230        240 
RTAAVFRPRP GDSLPSRETR YVELYVVVDN AEFQMLGSEA AVRHRVLEVV NHVDKLYQKL 

       250        260        270        280        290        300 
NFRVVLVGLE IWNSQDRFHV SPDPSVTLEN LLTWQARQRT RRHLHDNVQL ITGVDFTGTT 

       310        320        330        340        350        360 
VGFARVSAMC SHSSGAVNQD HSKNPVGVAC TMAHEMGHNL GMDHDENVQG CRCQERFEAG 

       370        380        390        400        410        420 
RCIMAGSIGS SFPRMFSDCS QAYLESFLER PQSVCLANAP DLSHLVGGPV CGNLFVERGE 

       430        440        450        460        470        480 
QCDCGPPEDC RNRCCNSTTC QLAEGAQCAH GTCCQECKVK PAGELCRPKK DMCDLEEFCD 

       490        500        510        520        530        540 
GRHPECPEDA FQENGTPCSG GYCYNGACPT LAQQCQAFWG PGGQAAEESC FSYDILPGCK 

       550        560        570        580        590        600 
ASRYRADMCG VLQCKGGQQP LGRAICIVDV CHALTTEDGT AYEPVPEGTR CGPEKVCWKG 

       610        620        630        640        650        660 
RCQDLHVYRS SNCSAQCHNH GVCNHKQECH CHAGWAPPHC AKLLTEVHAA SGSLPVLVVV 

       670        680        690        700        710        720 
VLVLLAVVLV TLAGIIVYRK ARSRILSRNV APKTTMGRSN PLFHQAASRV PAKGGAPAPS 

       730        740        750        760        770        780 
RGPQELVPTT HPGQPARHPA SSVALKRPPP APPVTVSSPP FPVPVYTRQA PKQVIKPTFA 

       790        800        810        820 
PPVPPVKPGA GAANPGPAEG AVGPKVALKP PIQRKQGAGA PTAP

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References

[1]	"CD156 (human ADAM8): expression, primary amino acid sequence, and gene location." Yoshiyama K., Higuchi Y., Kataoka M., Matsuura K., Yamamoto S. Genomics 41:56-62(1997) [PubMed: 9126482] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Blood.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	D26579 mRNA. Translation: BAA05626.1.
IPI	IPI00941231.
UniGene	Hs.501574
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P78325.
PTM databases
PhosphoSite	P78325.
Genome annotation databases
Ensembl	ENST00000368566; ENSP00000357554; ENSG00000151651; Homo sapiens. [Genome view]
KEGG	hsa:101.
Organism-specific databases
CTD	101.
GeneCards	GC10M134964.
H-InvDB	HIX0035404.
HGNC	HGNC:215. ADAM8.
MIM	602267. gene.
PharmGKB	PA24533.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P78325.
HOVERGEN	P78325.
Gene expression databases
ArrayExpress	P78325.
Bgee	P78325.
CleanEx	HS_ADAM8.
Genevestigator	P78325.
GermOnline	ENSG00000151651. Homo sapiens.
Family and domain databases
InterPro	IPR006586. ADAM_Cys-rich. IPR001762. Blood-coag_inhib_Disintegrin. IPR018358. Disintegrin_CS. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR013111. EGF_extracell. IPR001590. Peptidase_M12B. IPR002870. Peptidase_M12B_N. [Graphical view]
Gene3D	G3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit.
Pfam	PF08516. ADAM_CR. 1 hit. PF00200. Disintegrin. 1 hit. PF07974. EGF_2. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. [Graphical view]
PRINTS	PR00289. DISINTEGRIN.
SMART	SM00608. ACR. 1 hit. SM00050. DISIN. 1 hit. SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00427. DISINTEGRIN_1. 1 hit. PS50214. DISINTEGRIN_2. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	381.
SOURCE	Search...

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Entry information

Entry name

ADAM8_HUMAN

Accession

Primary (citable) accession number: P78325

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	November 24, 2009
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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