Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P78285

- LYSD_ECOLI

UniProt

P78285 - LYSD_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Lysozyme RrrD

Gene

rrrD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity. Exhibits lytic activity against E.coli and S.typhi cell wall substrate.1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei35 – 351Proton donorBy similarity
Active sitei44 – 441NucleophileBy similarity

GO - Molecular functioni

  1. lysozyme activity Source: EcoCyc

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. cytolysis by virus of host cell Source: EcoCyc
  3. defense response to bacterium Source: UniProtKB-KW
  4. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G6310-MONOMER.
ECOL316407:JW0544-MONOMER.
MetaCyc:G6310-MONOMER.

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysozyme RrrD (EC:3.2.1.17)
Alternative name(s):
Endolysin
Lysis protein
Muramidase
Gene namesi
Name:rrrD
Synonyms:arrD, ybcS
Ordered Locus Names:b0555, JW0544
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13635. rrrD.

Pathology & Biotechi

Disruption phenotypei

Mutants are unable to form wild-type biofilms, which could be due to altered peptidoglycan metabolism.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 165165Lysozyme RrrDPRO_0000218109Add
BLAST

Proteomic databases

PRIDEiP78285.

Expressioni

Gene expression databases

GenevestigatoriP78285.

Interactioni

Protein-protein interaction databases

IntActiP78285. 3 interactions.
STRINGi511145.b0555.

Structurei

3D structure databases

ProteinModelPortaliP78285.
SMRiP78285. Positions 1-165.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated

Phylogenomic databases

eggNOGiCOG3772.
HOGENOMiHOG000277068.
InParanoidiP78285.
KOiK01185.
OMAiGACRAIN.
OrthoDBiEOG60W7TZ.
PhylomeDBiP78285.

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
InterProiIPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamiPF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

P78285-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPSLRKAVA AAIGGGAIAI ASVLITGPSG NDGLEGVSYI PYKDIVGVWT
60 70 80 90 100
VCHGHTGKDI MLGKTYTKAE CKALLNKDLA TVARQINPYI KVDIPETTRG
110 120 130 140 150
ALYSFVYNVG AGNFRTSTLL RKINQGDIKG ACDQLRRWTY AGGKQWKGLM
160
TRREIEREVC LWGQQ
Length:165
Mass (Da):17,972
Last modified:February 1, 1997 - v1
Checksum:i370CECD5B419329B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U82598 Genomic DNA. Translation: AAB40751.1.
U00096 Genomic DNA. Translation: AAC73656.1.
AP009048 Genomic DNA. Translation: BAE76330.1.
PIRiA64788.
RefSeqiNP_415087.1. NC_000913.3.
YP_488842.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73656; AAC73656; b0555.
BAE76330; BAE76330; BAE76330.
GeneIDi12930891.
947539.
KEGGiecj:Y75_p0541.
eco:b0555.
PATRICi32116276. VBIEscCol129921_0578.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U82598 Genomic DNA. Translation: AAB40751.1 .
U00096 Genomic DNA. Translation: AAC73656.1 .
AP009048 Genomic DNA. Translation: BAE76330.1 .
PIRi A64788.
RefSeqi NP_415087.1. NC_000913.3.
YP_488842.1. NC_007779.1.

3D structure databases

ProteinModelPortali P78285.
SMRi P78285. Positions 1-165.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P78285. 3 interactions.
STRINGi 511145.b0555.

Protein family/group databases

CAZyi GH24. Glycoside Hydrolase Family 24.

Proteomic databases

PRIDEi P78285.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73656 ; AAC73656 ; b0555 .
BAE76330 ; BAE76330 ; BAE76330 .
GeneIDi 12930891.
947539.
KEGGi ecj:Y75_p0541.
eco:b0555.
PATRICi 32116276. VBIEscCol129921_0578.

Organism-specific databases

EchoBASEi EB3399.
EcoGenei EG13635. rrrD.

Phylogenomic databases

eggNOGi COG3772.
HOGENOMi HOG000277068.
InParanoidi P78285.
KOi K01185.
OMAi GACRAIN.
OrthoDBi EOG60W7TZ.
PhylomeDBi P78285.

Enzyme and pathway databases

BioCyci EcoCyc:G6310-MONOMER.
ECOL316407:JW0544-MONOMER.
MetaCyc:G6310-MONOMER.

Miscellaneous databases

PROi P78285.

Gene expression databases

Genevestigatori P78285.

Family and domain databases

Gene3Di 1.10.530.40. 1 hit.
InterProi IPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view ]
Pfami PF00959. Phage_lysozyme. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Subclassification and targeted characterization of prophage-encoded two-component cell lysis cassette."
    Srividhya K.V., Krishnaswamy S.
    J. Biosci. 32:979-990(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A LYSIN.
  5. "Role of DLP12 lysis genes in Escherichia coli biofilm formation."
    Toba F.A., Thompson M.G., Campbell B.R., Junker L.M., Rueggeberg K.G., Hay A.G.
    Microbiology 157:1640-1650(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076 / PHL626.

Entry informationi

Entry nameiLYSD_ECOLI
AccessioniPrimary (citable) accession number: P78285
Secondary accession number(s): Q2MBM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Encoded by the cryptic lambdoid prophage DLP12.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3