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P78285 (LYSD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysozyme RrrD

EC=3.2.1.17
Alternative name(s):
Endolysin
Lysis protein
Muramidase
Gene names
Name:rrrD
Synonyms:arrD, ybcS
Ordered Locus Names:b0555, JW0544
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length165 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity. Exhibits lytic activity against E.coli and S.typhi cell wall substrate. Ref.4

Catalytic activity

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Disruption phenotype

Mutants are unable to form wild-type biofilms, which could be due to altered peptidoglycan metabolism. Ref.5

Miscellaneous

Encoded by the cryptic lambdoid prophage DLP12.

Sequence similarities

Belongs to the glycosyl hydrolase 24 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 165165Lysozyme RrrD
PRO_0000218109

Sites

Active site351Proton donor By similarity
Active site441Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P78285 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 370CECD5B419329B

FASTA16517,972
        10         20         30         40         50         60 
MPPSLRKAVA AAIGGGAIAI ASVLITGPSG NDGLEGVSYI PYKDIVGVWT VCHGHTGKDI 

        70         80         90        100        110        120 
MLGKTYTKAE CKALLNKDLA TVARQINPYI KVDIPETTRG ALYSFVYNVG AGNFRTSTLL 

       130        140        150        160 
RKINQGDIKG ACDQLRRWTY AGGKQWKGLM TRREIEREVC LWGQQ 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Subclassification and targeted characterization of prophage-encoded two-component cell lysis cassette."
Srividhya K.V., Krishnaswamy S.
J. Biosci. 32:979-990(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A LYSIN.
[5]"Role of DLP12 lysis genes in Escherichia coli biofilm formation."
Toba F.A., Thompson M.G., Campbell B.R., Junker L.M., Rueggeberg K.G., Hay A.G.
Microbiology 157:1640-1650(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
Strain: K12 / MG1655 / ATCC 47076 / PHL626.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U82598 Genomic DNA. Translation: AAB40751.1.
U00096 Genomic DNA. Translation: AAC73656.1.
AP009048 Genomic DNA. Translation: BAE76330.1.
PIRA64788.
RefSeqNP_415087.1. NC_000913.3.
YP_488842.1. NC_007779.1.

3D structure databases

ProteinModelPortalP78285.
SMRP78285. Positions 1-165.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP78285. 3 interactions.
STRING511145.b0555.

Protein family/group databases

CAZyGH24. Glycoside Hydrolase Family 24.

Proteomic databases

PRIDEP78285.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73656; AAC73656; b0555.
BAE76330; BAE76330; BAE76330.
GeneID12930891.
947539.
KEGGecj:Y75_p0541.
eco:b0555.
PATRIC32116276. VBIEscCol129921_0578.

Organism-specific databases

EchoBASEEB3399.
EcoGeneEG13635. rrrD.

Phylogenomic databases

eggNOGCOG3772.
HOGENOMHOG000277068.
KOK01185.
OMAGIPTICE.
OrthoDBEOG60W7TZ.
PhylomeDBP78285.
ProtClustDBCLSK879758.

Enzyme and pathway databases

BioCycEcoCyc:G6310-MONOMER.
ECOL316407:JW0544-MONOMER.
MetaCyc:G6310-MONOMER.

Gene expression databases

GenevestigatorP78285.

Family and domain databases

Gene3D1.10.530.40. 1 hit.
InterProIPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamPF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
ProtoNetSearch...

Other

PROP78285.

Entry information

Entry nameLYSD_ECOLI
AccessionPrimary (citable) accession number: P78285
Secondary accession number(s): Q2MBM6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene