P78285 (LYSD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme RrrD
EC=3.2.1.17

Alternative name(s):
Endolysin
Lysis protein
Muramidase

Gene names

Name:	rrrD
Synonyms:	arrD, ybcS
Ordered Locus Names:	b0555, JW0544

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity. Exhibits lytic activity against E.coli and S.typhi cell wall substrate. Ref.4
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Disruption phenotype	Mutants are unable to form wild-type biofilms, which could be due to altered peptidoglycan metabolism. Ref.5
Miscellaneous	Encoded by the cryptic lambdoid prophage DLP12.
Sequence similarities	Belongs to the glycosyl hydrolase 24 family.

Ontologies

Keywords
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW lytic viral release Inferred from mutant phenotype Ref.4. Source: EcoCyc peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	lysozyme activity Inferred from direct assay Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Lysozyme RrrD		PRO_0000218109
Sites
	Active site	35	1	Proton donor By similarity
	Active site	44	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P78285 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 370CECD5B419329B	FASTA	165	17,972
	10 20 30 40 50 60 MPPSLRKAVA AAIGGGAIAI ASVLITGPSG NDGLEGVSYI PYKDIVGVWT VCHGHTGKDI 70 80 90 100 110 120 MLGKTYTKAE CKALLNKDLA TVARQINPYI KVDIPETTRG ALYSFVYNVG AGNFRTSTLL 130 140 150 160 RKINQGDIKG ACDQLRRWTY AGGKQWKGLM TRREIEREVC LWGQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Subclassification and targeted characterization of prophage-encoded two-component cell lysis cassette." Srividhya K.V., Krishnaswamy S. J. Biosci. 32:979-990(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A LYSIN.
[5]	"Role of DLP12 lysis genes in Escherichia coli biofilm formation." Toba F.A., Thompson M.G., Campbell B.R., Junker L.M., Rueggeberg K.G., Hay A.G. Microbiology 157:1640-1650(2011) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076 / PHL626.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82598 Genomic DNA. Translation: AAB40751.1. U00096 Genomic DNA. Translation: AAC73656.1. AP009048 Genomic DNA. Translation: BAE76330.1.
PIR	A64788.
RefSeq	NP_415087.1. NC_000913.2. YP_488842.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P78285.
SMR	P78285. Positions 1-165.
ModBase	Search...
Protein-protein interaction databases
IntAct	P78285. 3 interactions.
STRING	511145.b0555.
Protein family/group databases
CAZy	GH24. Glycoside Hydrolase Family 24.
Proteomic databases
PRIDE	P78285.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73656; AAC73656; b0555. BAE76330; BAE76330; BAE76330.
GeneID	12930891. 947539.
KEGG	ecj:Y75_p0541. eco:b0555.
PATRIC	32116276. VBIEscCol129921_0578.
Organism-specific databases
EchoBASE	EB3399.
EcoGene	EG13635. rrrD.
Phylogenomic databases
eggNOG	COG3772.
HOGENOM	HOG000277068.
KO	K01185.
OMA	RVGACKE.
ProtClustDB	CLSK879758.
Enzyme and pathway databases
BioCyc	EcoCyc:G6310-MONOMER. ECOL316407:JW0544-MONOMER. MetaCyc:G6310-MONOMER.
Gene expression databases
Genevestigator	P78285.
Family and domain databases
Gene3D	1.10.530.40. 1 hit.
InterPro	IPR002196. Glyco_hydro_24. IPR023346. Lysozyme-like_dom. IPR023347. Lysozyme_dom. [Graphical view]
Pfam	PF00959. Phage_lysozyme. 1 hit. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LYSD_ECOLI

Accession

Primary (citable) accession number: P78285
Secondary accession number(s): Q2MBM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program