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P78285

- LYSD_ECOLI

UniProt

P78285 - LYSD_ECOLI

Protein

Lysozyme RrrD

Gene

rrrD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity. Exhibits lytic activity against E.coli and S.typhi cell wall substrate.1 Publication

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei35 – 351Proton donorBy similarity
    Active sitei44 – 441NucleophileBy similarity

    GO - Molecular functioni

    1. lysozyme activity Source: EcoCyc

    GO - Biological processi

    1. cell wall macromolecule catabolic process Source: InterPro
    2. cytolysis by virus of host cell Source: EcoCyc
    3. defense response to bacterium Source: UniProtKB-KW
    4. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6310-MONOMER.
    ECOL316407:JW0544-MONOMER.
    MetaCyc:G6310-MONOMER.

    Protein family/group databases

    CAZyiGH24. Glycoside Hydrolase Family 24.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysozyme RrrD (EC:3.2.1.17)
    Alternative name(s):
    Endolysin
    Lysis protein
    Muramidase
    Gene namesi
    Name:rrrD
    Synonyms:arrD, ybcS
    Ordered Locus Names:b0555, JW0544
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13635. rrrD.

    Pathology & Biotechi

    Disruption phenotypei

    Mutants are unable to form wild-type biofilms, which could be due to altered peptidoglycan metabolism.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 165165Lysozyme RrrDPRO_0000218109Add
    BLAST

    Proteomic databases

    PRIDEiP78285.

    Expressioni

    Gene expression databases

    GenevestigatoriP78285.

    Interactioni

    Protein-protein interaction databases

    IntActiP78285. 3 interactions.
    STRINGi511145.b0555.

    Structurei

    3D structure databases

    ProteinModelPortaliP78285.
    SMRiP78285. Positions 1-165.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 24 family.Curated

    Phylogenomic databases

    eggNOGiCOG3772.
    HOGENOMiHOG000277068.
    KOiK01185.
    OMAiGACRAIN.
    OrthoDBiEOG60W7TZ.
    PhylomeDBiP78285.

    Family and domain databases

    Gene3Di1.10.530.40. 1 hit.
    InterProiIPR002196. Glyco_hydro_24.
    IPR023346. Lysozyme-like_dom.
    IPR023347. Lysozyme_dom.
    [Graphical view]
    PfamiPF00959. Phage_lysozyme. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P78285-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPSLRKAVA AAIGGGAIAI ASVLITGPSG NDGLEGVSYI PYKDIVGVWT    50
    VCHGHTGKDI MLGKTYTKAE CKALLNKDLA TVARQINPYI KVDIPETTRG 100
    ALYSFVYNVG AGNFRTSTLL RKINQGDIKG ACDQLRRWTY AGGKQWKGLM 150
    TRREIEREVC LWGQQ 165
    Length:165
    Mass (Da):17,972
    Last modified:February 1, 1997 - v1
    Checksum:i370CECD5B419329B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U82598 Genomic DNA. Translation: AAB40751.1.
    U00096 Genomic DNA. Translation: AAC73656.1.
    AP009048 Genomic DNA. Translation: BAE76330.1.
    PIRiA64788.
    RefSeqiNP_415087.1. NC_000913.3.
    YP_488842.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73656; AAC73656; b0555.
    BAE76330; BAE76330; BAE76330.
    GeneIDi12930891.
    947539.
    KEGGiecj:Y75_p0541.
    eco:b0555.
    PATRICi32116276. VBIEscCol129921_0578.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U82598 Genomic DNA. Translation: AAB40751.1 .
    U00096 Genomic DNA. Translation: AAC73656.1 .
    AP009048 Genomic DNA. Translation: BAE76330.1 .
    PIRi A64788.
    RefSeqi NP_415087.1. NC_000913.3.
    YP_488842.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P78285.
    SMRi P78285. Positions 1-165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P78285. 3 interactions.
    STRINGi 511145.b0555.

    Protein family/group databases

    CAZyi GH24. Glycoside Hydrolase Family 24.

    Proteomic databases

    PRIDEi P78285.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73656 ; AAC73656 ; b0555 .
    BAE76330 ; BAE76330 ; BAE76330 .
    GeneIDi 12930891.
    947539.
    KEGGi ecj:Y75_p0541.
    eco:b0555.
    PATRICi 32116276. VBIEscCol129921_0578.

    Organism-specific databases

    EchoBASEi EB3399.
    EcoGenei EG13635. rrrD.

    Phylogenomic databases

    eggNOGi COG3772.
    HOGENOMi HOG000277068.
    KOi K01185.
    OMAi GACRAIN.
    OrthoDBi EOG60W7TZ.
    PhylomeDBi P78285.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6310-MONOMER.
    ECOL316407:JW0544-MONOMER.
    MetaCyc:G6310-MONOMER.

    Miscellaneous databases

    PROi P78285.

    Gene expression databases

    Genevestigatori P78285.

    Family and domain databases

    Gene3Di 1.10.530.40. 1 hit.
    InterProi IPR002196. Glyco_hydro_24.
    IPR023346. Lysozyme-like_dom.
    IPR023347. Lysozyme_dom.
    [Graphical view ]
    Pfami PF00959. Phage_lysozyme. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Subclassification and targeted characterization of prophage-encoded two-component cell lysis cassette."
      Srividhya K.V., Krishnaswamy S.
      J. Biosci. 32:979-990(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A LYSIN.
    5. "Role of DLP12 lysis genes in Escherichia coli biofilm formation."
      Toba F.A., Thompson M.G., Campbell B.R., Junker L.M., Rueggeberg K.G., Hay A.G.
      Microbiology 157:1640-1650(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
      Strain: K12 / MG1655 / ATCC 47076 / PHL626.

    Entry informationi

    Entry nameiLYSD_ECOLI
    AccessioniPrimary (citable) accession number: P78285
    Secondary accession number(s): Q2MBM6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Encoded by the cryptic lambdoid prophage DLP12.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3