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Protein

Thiosulfate sulfurtransferase YnjE

Gene

ynjE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiosulfate + cyanide = sulfite + thiocyanate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei385Cysteine persulfide intermediatePROSITE-ProRule annotation1 Publication1
Binding sitei390SubstrateBy similarity1

GO - Molecular functioni

  • sulfurtransferase activity Source: EcoCyc
  • thiosulfate sulfurtransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G6952-MONOMER.
ECOL316407:JW5287-MONOMER.
MetaCyc:G6952-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiosulfate sulfurtransferase YnjE (EC:2.8.1.1)
Gene namesi
Name:ynjE
Ordered Locus Names:b1757, JW5287
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14007. ynjE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi385C → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23PROSITE-ProRule annotationAdd BLAST23
ChainiPRO_000003042924 – 435Thiosulfate sulfurtransferase YnjEAdd BLAST412

Proteomic databases

PaxDbiP78067.
PRIDEiP78067.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262126. 192 interactors.
DIPiDIP-12782N.
STRINGi511145.b1757.

Structurei

Secondary structure

1435
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 37Combined sources6
Beta strandi41 – 44Combined sources4
Helixi48 – 52Combined sources5
Beta strandi63 – 65Combined sources3
Helixi73 – 78Combined sources6
Helixi81 – 90Combined sources10
Beta strandi99 – 103Combined sources5
Helixi105 – 117Combined sources13
Beta strandi123 – 126Combined sources4
Turni127 – 130Combined sources4
Helixi133 – 135Combined sources3
Helixi142 – 144Combined sources3
Helixi148 – 155Combined sources8
Beta strandi168 – 177Combined sources10
Helixi180 – 183Combined sources4
Beta strandi190 – 193Combined sources4
Helixi194 – 196Combined sources3
Turni200 – 203Combined sources4
Helixi208 – 217Combined sources10
Beta strandi224 – 229Combined sources6
Helixi233 – 246Combined sources14
Beta strandi251 – 254Combined sources4
Turni255 – 257Combined sources3
Helixi258 – 263Combined sources6
Helixi290 – 292Combined sources3
Helixi296 – 300Combined sources5
Turni301 – 304Combined sources4
Beta strandi306 – 312Combined sources7
Helixi316 – 319Combined sources4
Beta strandi333 – 335Combined sources3
Helixi351 – 353Combined sources3
Beta strandi358 – 360Combined sources3
Helixi363 – 371Combined sources9
Turni372 – 374Combined sources3
Beta strandi379 – 384Combined sources6
Beta strandi386 – 388Combined sources3
Helixi389 – 400Combined sources12
Beta strandi404 – 411Combined sources8
Helixi412 – 416Combined sources5
Helixi431 – 434Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WLRX-ray1.45A24-435[»]
2WLXX-ray1.90A24-435[»]
3IPOX-ray2.40A/B20-435[»]
3IPPX-ray2.40A/B20-435[»]
ProteinModelPortaliP78067.
SMRiP78067.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78067.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 138Rhodanese 1PROSITE-ProRule annotationAdd BLAST103
Domaini164 – 270Rhodanese 2PROSITE-ProRule annotationAdd BLAST107
Domaini304 – 425Rhodanese 3PROSITE-ProRule annotationAdd BLAST122

Sequence similaritiesi

Contains 3 rhodanese domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4107RM6. Bacteria.
COG2897. LUCA.
HOGENOMiHOG000282520.
InParanoidiP78067.
KOiK01011.
OMAiYMGVKDV.
PhylomeDBiP78067.

Family and domain databases

Gene3Di3.40.250.10. 3 hits.
InterProiIPR001763. Rhodanese-like_dom.
IPR001307. Thiosulphate_STrfase_CS.
[Graphical view]
PfamiPF00581. Rhodanese. 2 hits.
[Graphical view]
SMARTiSM00450. RHOD. 3 hits.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 3 hits.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS00380. RHODANESE_1. 1 hit.
PS00683. RHODANESE_2. 1 hit.
PS50206. RHODANESE_3. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P78067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRVSQMTAL AMALGLACAS SWAAELAKPL TLDQLQQQNG KAIDTRPSAF
60 70 80 90 100
YNGWPQTLNG PSGHELAALN LSASWLDKMS TEQLNAWIKQ HNLKTDAPVA
110 120 130 140 150
LYGNDKDVDA VKTRLQKAGL THISILSDAL SEPSRLQKLP HFEQLVYPQW
160 170 180 190 200
LHDLQQGKEV TAKPAGDWKV IEAAWGAPKL YLISHIPGAD YIDTNEVESE
210 220 230 240 250
PLWNKVSDEQ LKAMLAKHGI RHDTTVILYG RDVYAAARVA QIMLYAGVKD
260 270 280 290 300
VRLLDGGWQT WSDAGLPVER GTPPKVKAEP DFGVKIPAQP QLMLDMEQAR
310 320 330 340 350
GLLHRQDASL VSIRSWPEFI GTTSGYSYIK PKGEIAGARW GHAGSDSTHM
360 370 380 390 400
EDFHNPDGTM RSADDITAMW KAWNIKPEQQ VSFYCGTGWR ASETFMYARA
410 420 430
MGWKNVSVYD GGWYEWSSDP KNPVATGERG PDSSK
Length:435
Mass (Da):48,229
Last modified:December 15, 1998 - v2
Checksum:iC66CCFB50EC464BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74827.2.
AP009048 Genomic DNA. Translation: BAA15548.2.
PIRiE64935.
RefSeqiNP_416271.4. NC_000913.3.
WP_001350515.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74827; AAC74827; b1757.
BAA15548; BAA15548; BAA15548.
GeneIDi946505.
KEGGiecj:JW5287.
eco:b1757.
PATRICi32118825. VBIEscCol129921_1830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74827.2.
AP009048 Genomic DNA. Translation: BAA15548.2.
PIRiE64935.
RefSeqiNP_416271.4. NC_000913.3.
WP_001350515.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WLRX-ray1.45A24-435[»]
2WLXX-ray1.90A24-435[»]
3IPOX-ray2.40A/B20-435[»]
3IPPX-ray2.40A/B20-435[»]
ProteinModelPortaliP78067.
SMRiP78067.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262126. 192 interactors.
DIPiDIP-12782N.
STRINGi511145.b1757.

Proteomic databases

PaxDbiP78067.
PRIDEiP78067.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74827; AAC74827; b1757.
BAA15548; BAA15548; BAA15548.
GeneIDi946505.
KEGGiecj:JW5287.
eco:b1757.
PATRICi32118825. VBIEscCol129921_1830.

Organism-specific databases

EchoBASEiEB3763.
EcoGeneiEG14007. ynjE.

Phylogenomic databases

eggNOGiENOG4107RM6. Bacteria.
COG2897. LUCA.
HOGENOMiHOG000282520.
InParanoidiP78067.
KOiK01011.
OMAiYMGVKDV.
PhylomeDBiP78067.

Enzyme and pathway databases

BioCyciEcoCyc:G6952-MONOMER.
ECOL316407:JW5287-MONOMER.
MetaCyc:G6952-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP78067.
PROiP78067.

Family and domain databases

Gene3Di3.40.250.10. 3 hits.
InterProiIPR001763. Rhodanese-like_dom.
IPR001307. Thiosulphate_STrfase_CS.
[Graphical view]
PfamiPF00581. Rhodanese. 2 hits.
[Graphical view]
SMARTiSM00450. RHOD. 3 hits.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 3 hits.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS00380. RHODANESE_1. 1 hit.
PS00683. RHODANESE_2. 1 hit.
PS50206. RHODANESE_3. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYNJE_ECOLI
AccessioniPrimary (citable) accession number: P78067
Secondary accession number(s): P78171
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In vitro, YnjE can be efficiently persulfurated by the cysteine desulfurase IscS.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.