Fructose-6-phosphate aldolase 1 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P78055 (FSAA_ECOLI)

Last modified November 24, 2009. Version 86. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Fructose-6-phosphate aldolase 1
EC=4.1.2.-

Gene names

Name:	fsaA
Synonyms:	fsa, mipB, ybiZ
Ordered Locus Names:	b0825, JW5109

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	D-fructose 6-phosphate = glycerone + D-glyceraldehyde 3-phosphate. HAMAP MF_00496
Enzyme regulation	Inhibited by glycerol, inorganic phosphate and arabinose 5-phosphate. HAMAP MF_00496
Subunit structure	Homodecamer or homododecamer. HAMAP MF_00496
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the transaldolase family. Type 3A subfamily.
Mass spectrometry	Molecular mass is 22998 Da from positions 1 - 220. Determined by ESI. Ref.5

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Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	fructose metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aldehyde-lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 220

220

Fructose-6-phosphate aldolase 1 HAMAP MF_00496

PRO_0000173643

Sites

Active site

HAMAP MF_00496

Experimental info

Mutagenesis

K → R: Almost complete loss of activity. Ref.5

Secondary structure

220

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P78055-1 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 317B9A06CD19C587
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FASTA

220

22,997

        10         20         30         40         50         60 
MELYLDTSDV VAVKALSRIF PLAGVTTNPS IIAAGKKPLD VVLPQLHEAM GGQGRLFAQV 

        70         80         90        100        110        120 
MATTAEGMVN DALKLRSIIA DIVVKVPVTA EGLAAIKMLK AEGIPTLGTA VYGAAQGLLS 

       130        140        150        160        170        180 
ALAGAEYVAP YVNRIDAQGG SGIQTVTDLH QLLKMHAPQA KVLAASFKTP RQALDCLLAG 

       190        200        210        220 
CESITLPLDV AQQMISYPAV DAAVAKFEQD WQGAFGRTSI

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Isomura M., Ogino T., Mizuno T. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Fructose-6-phosphate aldolase is a novel class I aldolase from Escherichia coli and is related to a novel group of bacterial transaldolases." Schuermann M., Sprenger G.A. J. Biol. Chem. 276:11055-11061(2001) [PubMed: 11120740] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION, MASS SPECTROMETRY, MUTAGENESIS OF LYS-85. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

D88188 Genomic DNA. Translation: BAA13552.1.
U00096 Genomic DNA. Translation: AAC73912.1. Different initiation.
AP009048 Genomic DNA. Translation: BAA35513.2.

PIR

A64820.

RefSeq

AP_001456.1.
NP_415346.4.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1L6W	X-ray	1.93	A/B/C/D/E/F/G/H/I/J	1-220	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:10218N.

STRING

P78055.

Genome annotation databases

GeneID

945449.

GenomeReviews

Gene locus JW5109 in contig AP009048_GR.
Gene locus b0825 in contig U00096_GR.

KEGG

ecj:JW5109.
eco:b0825.

Organism-specific databases

EchoBASE

EB3244.

EcoGene

EG13471. fsaA.

CMR

Search...

Phylogenomic databases

HOGENOM

P78055.

OMA

MSAANID

Enzyme and pathway databases

BioCyc

EcoCyc:G6428-MON.
ECOL168927:B0825-MON.
MetaCyc:G6428-MON.

Gene expression databases

Genevestigator

P78055.

Family and domain databases

HAMAP

MF_00496.
[Tree]

InterPro

IPR013785. Aldolase_TIM.
IPR001585. Transaldolase.
IPR018225. Transaldolase_AS.
IPR004731. Transaldolase_C.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

PANTHER

PTHR10683. Transaldolase. 1 hit.

Pfam

PF00923. Transaldolase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00875. talC. 1 hit.

PROSITE

PS01054. TRANSALDOLASE_1. 1 hit.
PS00958. TRANSALDOLASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

FSAA_ECOLI

Accession

Primary (citable) accession number: P78055
Secondary accession number(s): P77855, Q9R3X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	November 24, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families